IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hori-2008-3 CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore. chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm ATCC Number:CRL-2111 chicken bursa lymphoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hori T., Amano M., Suzuki A., Backer CB., Welburn JP., Dong Y., McEwen BF., Shang WH., Suzuki E., Okawa K., Cheeseman IM., Fukagawa T. tfukagaw@lab.nig.ac.jp Cell (0092-8674) 2008 Only protein-protein interactions 9 imex curation hori-2008-4 CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore. chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm ATCC Number:CRL-2111 chicken bursa lymphoma fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Hori T., Amano M., Suzuki A., Backer CB., Welburn JP., Dong Y., McEwen BF., Shang WH., Suzuki E., Okawa K., Cheeseman IM., Fukagawa T. tfukagaw@lab.nig.ac.jp Cell (0092-8674) 2008 Only protein-protein interactions 9 imex curation hori-2008-2 CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore. chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm ATCC Number:CRL-2111 chicken bursa lymphoma anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Hori T., Amano M., Suzuki A., Backer CB., Welburn JP., Dong Y., McEwen BF., Shang WH., Suzuki E., Okawa K., Cheeseman IM., Fukagawa T. Source of bait proteins supplied by the author tfukagaw@lab.nig.ac.jp Cell (0092-8674) 2008 Only protein-protein interactions 9 imex curation hori-2008-1 CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore. chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm ATCC Number:CRL-2111 chicken bursa lymphoma molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Hori T., Amano M., Suzuki A., Backer CB., Welburn JP., Dong Y., McEwen BF., Shang WH., Suzuki E., Okawa K., Cheeseman IM., Fukagawa T. tfukagaw@lab.nig.ac.jp Cell (0092-8674) 2008 Only protein-protein interactions 9 imex curation cenpt_chick Centromere protein T CENPT protein protein chick Gallus gallus (Chicken) Chicken MDGRVGLRASRRAAPTPRVAVRSSPRHRARVREQEPVVSAMSGSGLGKENKAGSSTPLRHRPNAFSELDNATPRVMLRKIIQNQPQVSPLALQTVQLEETEDARPEPPSQRTSSTVELQLPDLVPEDASVTTFRMTRKRKKLSISEFERAADKRLPQNQAHSTLDSTLVRSLRMSVGSVMAPDTVEKRGLLRRPQNHKAIDIAAFEGGVEQNMLQIKAQDYLVDLQTSSMTGTTTIRTDAEVVLNNTELFVEPQLGEQNLLAVEPQLSDSKTSAQRSNTSYPAHEKARLEGLVSRVSTDERRTLRFSEKDLITDHEHVDGITQKTPAKQGEEEQDHSQQNDPMEQFSESEEMAGTTEHHADAEYSEHSEKKLSRKAVSQLTAAQDAGVEMEMTPSEGGVAEGTEHQDSPKAELQMAGSPGGHSPASYSLEKPGAKPLKEAVEQTGEIERGTITGVLDAAEEEATDDESDKEDHESEEISMKTPMFVHAAAYRPQPVLSPPHPVKSASPELPPQPVRAKPVPKSSGAAQRKTREPEIASSLIKQIFSHYVKTPVTRDAYKIVEKCSERYFKQISSDLEAYSQHAGRKTVEMADVELLMRRQGLVTDKMPLHVLVERHLPLEYRKLLIPIAVSGNKVIPCK cenpw_chick Centromere protein W CENPW protein protein chick Gallus gallus (Chicken) Chicken MRRTVPRGTLRKIIKKHKPHLRLAANTDLLVHLSFLLFLHRLAEEARTNAFENKSKIIKPEHTIAAAKVILKKSRG cenpa_chick Histone H3-like centromeric protein A CENPA Centromere protein A protein protein chick Gallus gallus (Chicken) Chicken MPRPKPRSPRRRGRPPPAAPPPPPARPRARRYRPGQRALREIRRYQSSTALLLRRQPFARVVREICLLFTRGVDYRWQAMALLALQEAAEAFLVHLLEDAYLCSLHARRVTLYPKDLQLARRLRGLQGEGF o57392_chick centoromere protein C CENP-C protein protein chick Gallus gallus (Chicken) Chicken MAERLDHLKKYYRARFCGQGGKKINIQPGQNVLKYIQDCFECDDTDVTINSPNATSSSTPLVRNQERTSTQSPRPDAGLTIVVGNRTCHSTVSLPATPAETVRSKGEPCVFCPEPGRNSRIADSIKTDSPVHRGETQVLTFKDVEGLCESPAKLSDPEDDPGVVGSPLLVEEAKTPVQTRTVSIAPSSPSPVKDHNVEIESDCEFLIDESGCLSFKSWFSIPRKNRKSQKDGSAAPVLKSQSSDKEKTADKKGKNMNVQAEVHSEQKTKNVRVQRDLKRTSESHPVSSDSEGKVLKSKMQSSTHAGKSKKDALKGSSKHRKKMSYESEAEQLMLSGLDSETSDGEQKIRRVMPREDLAVPSAEHQQEQMLSSKKNLTPCKYQQSASKAYQRSVHKKQTKQTLANDTVLKSQRKKLKKSGKRNGNKNPEPRRWKSSDSEPGEEGLEREPLESNEMFTSPPHQELQTSVLQKLAKSEKAEKVLHALESVTDVNNKTPLKAVELLQHLVDSMKNSEKKRSSVTSSGKMLKKVHHRAHKRACSITRDSEPQNTTDSDSSSAPEVARKKRRQSAVRASKKPKSNRHQRLQHSPAAEKAMGPESGLVLEHSEKYTSPSKHCEENNESSDNSEDLHCQIKNLLSDEIGRQKIVLPSNTPNVRRTKRIRLKPLEYWRGERVTYTLKPSGRLLISGIAGAEAEPHMKNKLKSRHKKKRAKTRSEVVKCLDIPLADASKPTSIVDPVTNQEVLLECINSGSSHSCFFEDESIKVYKSLNTSDFAAGKLILKPLKEKGHQFVHMDTIAFYVIRGQIITTLHKTSYYLTSGDYFYVPAGNGYNIRNLLNEESVLHFTQLKNDRAPVGAELCSVTNENWSLEGKS cenph_chick Centromere protein H CENPH protein protein chick Gallus gallus (Chicken) Chicken MAGRLSESVGSGPGAEAETAADPDAKRDVLEHLCARTHLKQLVMEFDTACPPDEGCNSGAEVNFIESAKETLEEVGKVKSAFESKALVIKRIQLMDALRKRVKENDGCARLIVETMRDIIKLNWEIIQAHQQARVIRENLNDIRRKRYFLKQAEGEKALRIFTTVRKKKEVVRMKIAEKLKFIHRNVQYERKVTTLVQNILQNIIVGCQINWAKDPSLRAIILQLEKDISIQNLL cenpn_chick Centromere protein N CENPN protein protein chick Gallus gallus (Chicken) Chicken MDEVIVEYIRRTVLKIPRDEIMAVLQKWGFLSEAQLQTINFRQTKEGISHSVAQLCEESSADLKQAALLDIIYNHIYPNKRVWSVYHMNKTGEETDFFDFRDFKKKFRRQIQSALINVTINFREYEDNAIWIRIAWGTPYTKPNQYKTSYVVYHSQTPYVFISASVLRSNLPLLCQAMVVASNYHDIHEMELRSHCLNSLKDIVFKRYSQNFQTNYPLQERNVLTENVDLRINDENRSEKERIYRLNQESFGNGPQPKLDFAQYKLETMFKSDPKWDVLEKKEPFRCLVKFSSPHLLESLKSLAPAGLADAPLSPLLTCIPQKARNYFKIREKKSLHPGSFVSP cenpk_chick Centromere protein K CENPK protein protein chick Gallus gallus (Chicken) Chicken MSDSVCPIDAKEELLRECENIWKEMEKCQSKLTLLAAEPVPESDAKVSLLLTRMQALRAEYHQWQKRNPELISTNPEVLLLLGEEELQKVKKELEMVLSAVQLKNEKLKEDLEREQQWHDEQVQILNAFKEIEEEMKKEVVTDSEKRVFQELKNQMLELKEYKKKLMNALGEFLEEHFPLPEKNGNAKKKRYSEEPPEQVITIHEILEILLNQLMSTPHEPYVTVDDSFWPPYLELLLRSGIVLRHPEDPNRIRLEAFHE cenpi_chick Centromere protein I CENPI protein protein chick Gallus gallus (Chicken) Chicken MQRRQSSKHSKRPLQVHHSNQTDLSAWRKGGTVDTEKSAQNRQSLSDQKNDNEQDSLEQALSYFEKIQDRVSLKKSEVLQKHLSTMESIALKRGLPPEGFDVLLDVALSGKLADTVNTRLLKSLIPASAIPESSIVSSVSWFCVSKCSSNIQLLFLRWLITMFDFIDHKEQVHALYGIFFSFLNDEKLCPYICHVLYLLTRKENVKPFRVRRLLDLQSKMGMQPHLQALLSLYKLFCPELVSITLPQKMKTYFKNADGPWKAAINAVRQRNQANSTVPQPLLLGTAQPHSRKRKWNTQLIVPASSANAQNLVVGGKMSRADSYSANESFPVEQLRTFPQLLQNIHRLEFPSQMGSVLTNPLLLHYMNCSKDESVYLRLYYWMGQTLQEECTWCVVDNNQYEEEFRGFLETVYKAECFLQEGFPSCEEFLYRSLPLWDGVSCRSQILQLVSWIPLSTFSEMKSQLCDPLAQLFFTSSLYFKCSVLESLKELLQNWLNWHVVQLDSESDSQFSSLNTTLSGLVNGVAELINFVGRISTAALHLEKSHTFLLYFILDFYETVCDIYLKYKLPLLIMPPAGVFYPALLSMDSVNLNQLCYIMYRYRTNLIAAKENEMSKKKIQQFKFSSQTYQEYNQYIIAMVGCLWTSSAFQKDNHPEGIRLDDELLKKTGVREYKNSFNIVYHPALMCYAVDFLQQAWPDDTTFNFNLIKGKKWNWYLRYLYGQGLEGLKLFIESSINRVSKASQSKAEDEDEKV cenpl_chick Centromere protein L CENPL RCJMB04_9b13 protein protein chick Gallus gallus (Chicken) Chicken MAGGRPAGSAIEMEGAMRTLPSSGRPSGTGWQSRGPLFGRNYGLSPGSRLLPPRCQENADPQKMAFLLRKQWTLYSVSPLYKFSSADLKDYARMLGVFIAAEKQKGLAVDVDGELGIKVAMSGLPELRGSEQDPAAVLVQLSLRSSVSPKNSEEKLIWSGWFCCVAGDDLLENLPENFTCLPLFLVNGAESYTAIVGSWFQKTFDCCFRRLAISPLNLTWMAAMWTGCKVDKNTAATELLFSVPHLPQPLDISYAIHPEDAKALWDTVQKTPGEITQEEVDVFMECLYSHFHRHFKIHLSATKLVKVSTGIASAHCDGIIKFLQSQYLIGVLMLLTELAISQIQ cenpm_chick Centromere protein M CENPM protein protein chick Gallus gallus (Chicken) Chicken MAVLRPFDKLPELNSATILLVGAEGGPQQQLAEAMLREKKDFNINIHLASSLPLPSERDHLRPRIDLIAFVIDIKSKYSLKNVQASLAYVDVRFFLGKVCFLVTGVGRANNCSVEMNAIWKLGEDYCSPVIFCELELEGIRVATAQRLLRMLQICAGRVPGVSALYFSLLMRNSAGD cenpt-cug2-2 Interaction between CUG2 and CENPT identified by coimmunoprecipitation 786706 n/a 786710 unspecified role unspecified role prey prey n/a 786711 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1C cenpt-cenpa-2 Interaction between CENPA and CENPT identified by coimmunoprecipitation 786706 n/a 786710 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 786712 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3G Interaction only observed when cellular chromatin is partially digested cenpc-cenpa-2 Interaction between CENPA and CENPC identified by coimmunoprecipitation 786706 n/a 786713 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 786712 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3G Interaction only observed when cellular chromatin is partially digested cenpc-cug2-3 Colocaization of CUG2 and CENPC 786707 n/a 786713 unspecified role unspecified role neutral component neutral component n/a 786711 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 1D cenpc-cenpa-1 Colocaization of CENPA and CENPC 786707 n/a 786713 unspecified role unspecified role neutral component neutral component n/a 786712 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 5A this interaction occurred in CENP-A-deficient cells. cenpt-cenpa-1 Colocaization of CENPA and CENPT 786707 n/a 786712 unspecified role unspecified role neutral component neutral component n/a 786710 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 5A cenpt-cug2-1 Interactors of CENPT 786708 n/a 786711 unspecified role unspecified role prey prey n/a 786710 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 786710 unspecified role unspecified role prey prey association association false false false Histones H3, H2A/H2B and H4 were also present but could not be unambiguously assigned as a single gene product Fig. 1B cenph-cenpt-2 Interactors of CENP-H 786708 n/a 786714 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 786715 unspecified role unspecified role prey prey n/a 786716 unspecified role unspecified role prey prey n/a 786717 unspecified role unspecified role prey prey n/a 786718 unspecified role unspecified role prey prey n/a 786719 unspecified role unspecified role prey prey association association false false false Profiles did not fully overlap, suggesting that CENP-H and CENP-T may belong to separate sub-complexes Fig. 1B cenph-cenpt-1 Cofractionation of CENP-T and CENP-H 786709 n/a 786714 unspecified role unspecified role neutral component neutral component n/a 786710 unspecified role unspecified role neutral component neutral component physical association physical association false false false Profiles did not fully overlap, suggesting that CENP-H and CENP-T may belong to separate sub-complexes Fig. 1A