UniProt UniProt http://www.uniprot.org gregan-2007-1 The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Gregan J., Riedel CG., Pidoux AL., Katou Y., Rumpf C., Schleiffer A., Kearsey SE., Shirahige K., Allshire RC., Nasmyth K. juraj.gregan@univie.ac.at Curr. Biol. (0960-9822) 2007 Only protein-protein interactions imex curation gregan-2007-2 The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Gregan J., Riedel CG., Pidoux AL., Katou Y., Rumpf C., Schleiffer A., Kearsey SE., Shirahige K., Allshire RC., Nasmyth K. juraj.gregan@univie.ac.at Curr. Biol. (0960-9822) 2007 Only protein-protein interactions imex curation mde4_schpo Monopolin complex subunit mde4 mde4 Mei4-dependent protein 4 SPBC6B1.04 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSTISTSTDSKLDNLGLSVTSRRNQILFYLSKALNLAHLLRSDSLQKSFLDALKQSATDSELLHKNLDEIKFLQNEKLNNEKLLEQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPEKHEDAVSQNRLRGSLDTVSSPSKTHKANKDEKATRLHLIIANLKKALKEKDAEVLNLQSHVSSKESELDRFKIKLETEESNWKVRLQVLESKLATQDRKLRMQKKSTERKSLLVSPRVSSPKLFSPSKQAIMGTRQPNATSGSPLSVTPFLQKTSTSIGLSSSPPQSSPSAQSSQPFSRDKYPHSMTVSPSNARYLKKHLDDTIPSNVSDINHNDHLKIPQSPSSLSPSKIPIRKKRKLKDTVSNCEFTEEDSESSFLLETIQPTKSTLRRSISPLKKRNDEINELKKGFTMKK 9D26F6A1BAAC5601 pcs1_schpo Monopolin complex subunit pcs1 pcs1 Chromosome segregation protein 1 SPAC11E3.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MRKNNMQTSKDSELKEQAKGKSSKLIHKLPKQRTRISQGQMHSTQDFVNNEDQDAYSVRENENELHINNSGMSELNKKLQLPNVELSTLSHTQEQEFNELNKLIRKINELQEFYLLEDLAKPVTNAGADADDTIVKDLKKELENEKKANHSLKNELLKTREQIKNYSKINILIKELFGLEVADCIEDEDGYRFNCKNTGRRGTLEYQLLLDDQNFTFTPRLNVQTDEELMKHLPDYLLEEIIFTKEQGKLFSARLMKALQD 788A079D2920CE35 flp1_schpo Tyrosine-protein phosphatase CDC14 homolog flp1 CDC fourteen-like phosphatase 1 clp1 SPAC1782.09c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDYQDDGLGEMIEFLEDKLYYTSLSQPPKAELYPHMHFFTIDDELIYNPFYHDFGPLNVSHLIRFAVIVHGIMGKHRQAKKSKAIVLYSSTDTRLRANAACLLACYMVLVQNWPPHLALAPLAQAEPPFLGFRDAGYAVSDYYITIQDCVYGLWRARESSILNIRNIDVHDYETYERVENGDFNWISPKFIAFASPIQAGWNHASTRPKKLPQPFAIVLDYFVANKVKLIVRLNGPLYDKKTFENVGIRHKEMYFEDGTVPELSLVKEFIDLTEEVEEDGVIAVHCKAGLGRTGCLIGAYLIYKHCFTANEVIAYMRIMRPGMVVGPQQHWLHINQVHFRAYFYEKAMGRAIQQATAAEPLATPPRHPLNATNGTSQSNISTPLPEPTPGQPRKVSGHNPPSARRLPSASSVKFNEKLKNASKQSIQNENKASYSSYEDSEIQNDDETRTVGTPTETISVVRLRRSSSQSNIEPNGVRSPTSSPTGSPIRRTSGNRWSSGSSHSKKSAQRSVSMSSLNNTSNGRVAKPKPSKSRLIS nuf2_schpo Kinetochore protein nuf2 NMS complex subunit nuf2 nuf2 SPAC27F1.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MARKHTFPSLKRAEILECIDGLGIPFTAKELDQPTSKAVIPLYEEFLDLFMGLTRQNLEEKVNSLQDSVENFEIIHESLRFTVFYQILSQFMQNICFHDFTIQDLLKPDRNRLQLILSAVINFAKLREERLQQFDDDIQKRESLLETYTLLDAQRKDLEEKVLLSQDRKLESEAIIKQNEERNEEMFQSLIEDKRLCSQVRTEYDRIRMEASELKIRYHNVDSLMASTLEEIEKLQSSIVHSPEKLKGKIADTSLRIQNDRSQQVELDKKSKILHTKLNSLQLIEGDLNACLKVLEECLVELDKLEHATVLLSTNQELCDQIEINKKKLEFRKEQLLKQLSNAQEKLEHEQHSRNQKLEAAKQRMDNIREEYKVITQERNKKIQETEKKNAMIEMTEQKIAGMREELESQISSITMEFEKLKSHVELYIAELLRNLRSSNS D716FE83223BD16E cenpa_schpo Histone H3-like centromeric protein cnp1 cnp1 sim2 SPBC1105.17 CENP-A homolog Silencing in the middle of the centromere protein 2 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAKKSLMAEPGDPIPRPRKKRYRPGTTALREIRKYQRSTDLLIQRLPFSRIVREISSEFVANFSTDVGLRWQSTALQCLQEAAEAFLVHLFEDTNLCAIHAKRVTIMQRDMQLARRIRGA mis12_schpo Centromere protein mis12 NMS complex subunit mis12 mis12 SPBC409.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLVELLEFTPLSFIDDVINITNQLLYKGVNGVDKAFSQTRFAKKAPQEIEEGLHKFEVLFESVVDRYYDGFEVYTLRNIFSYPPELKGYMRTFGKDVDYSITTEQDAAMDQAIQEAAEKLVVKMQLRRDLRMRLSRKREKKTEIEKHLERISFLNKVPENWQVTLPETTDFLLDQLGNLQHAVKRVVEASPTVHSREVDERITYLEKGYERLSNPIDQQKDFWSHHLSKLESTANTETANNIHKLLLSSEKDVGHTDEP pcs1-mde4-1 793143 n/a 793145 unspecified role unspecified role prey prey n/a 793146 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 1A, 1B Interaction occurs in mitosis and meiosis. pcs1-clp1-1 793143 n/a 793146 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 793147 unspecified role unspecified role prey prey physical association physical association false false false Fig 1B Interaction detected in meiosis. cnp1-nuf2-1 793144 n/a 793148 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 793149 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 4K, 4L, 4M cnp1-mis12-1 793144 n/a 793150 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 793149 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 4N, 4O