IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ panasenko-2006-1 The yeast Ccr4-Not complex controls ubiquitination of the nascent-associated polypeptide (NAC-EGD) complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Panasenko O., Landrieux E., Feuermann M., Finka A., Paquet N., Collart MA. martine.collart@medecine.unige.ch J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation panasenko-2006-3 The yeast Ccr4-Not complex controls ubiquitination of the nascent-associated polypeptide (NAC-EGD) complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Panasenko O., Landrieux E., Feuermann M., Finka A., Paquet N., Collart MA. martine.collart@medecine.unige.ch J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation panasenko-2006-2 The yeast Ccr4-Not complex controls ubiquitination of the nascent-associated polypeptide (NAC-EGD) complex. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Panasenko O., Landrieux E., Feuermann M., Finka A., Paquet N., Collart MA. martine.collart@medecine.unige.ch J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation not1_yeast General negative regulator of transcription subunit 1 CDC39 NOT1 ROS1 YCR093W YCR93W YCR1151 Cell division cycle protein 39 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSATYRDLNTASNLETSKEKQAAQIVIAQISLLFTTLNNDNFESVEREIRHILDRSSVDIYIKVWERLLTLSSRDILQAGKFLLQENLLHRLLLEFAKDLPKKSTDLIELLKERTFNNQEFQKQTGITLSLFIDLFDKSANKDIIESLDRSSQINDFKTIKMNHTNYLRNFFLQTTPETLESNLRDLLHSLEGESLNDLLALLLSEILSPGSQNLQNDPTRSWLTPPMVLDATNRGNVIARSISSLQANQINWNRVFNLMSTKYFLSAPLMPTTASLSCLFAALHDGPVIDEFFSCDWKVIFKLDLAIQLHKWSVQNGCFDLLNAEGTRKVSETIPNTKQSLLYLLSIASLNLELFLQREELSDGPMLAYFQECFFEDFNYAPEYLILALVKEMKRFVLLIENRTVIDEILITLLIQVHNKSPSSFKDVISTITDDSKIVDAAKIIINSDDAPIANFLKSLLDTGRLDTVINKLPFNEAFKILPCARQIGWEGFDTFLKTKVSPSNVDVVLESLEVQTKMTDTNTPFRSLKTFDLFAFHSLIEVLNKCPLDVLQLQRFESLEFSLLIAFPRLINFGFGHDEAILANGDIAGINNDIEKEMQNYLQKMYSGELAIKDVIELLRRLRDSDLPRDQEVFTCITHAVIAESTFFQDYPLDALATTSVLFGSMILFQLLRGFVLDVAFRIIMRFAKEPPESKMFKFAVQAIYAFRIRLAEYPQYCKDLLRDVPALKSQAQVYQSIVEAATLANAPKERSRPVQEMIPLKFFAVDEVSCQINQEGAPKDVVEKVLFVLNNVTLANLNNKVDELKKSLTPNYFSWFSTYLVTQRAKTEPNYHDLYSKVIVAMGSGLLHQFMVNVTLRQLFVLLSTKDEQAIDKKHLKNLASWLGCITLALNKPIKHKNIAFREMLIEAYKENRLEIVVPFVTKILQRASESKIFKPPNPWTVGILKLLIELNEKANWKLSLTFEVEVLLKSFNLTTKSLKPSNFINTPEVIETLSGALGSITLEQQQTEQQRQIILMQQHQQQMLIYQQRQQQQQQRQQQQQHHISANTIADQQAAFGGEGSISHDNPFNNLLGSTIFVTHPDLKRVFQMALAKSVREILLEVVEKSSGIAVVTTTKIILKDFATEVDESKLKTAAIIMVRHLAQSLARATSIEPLKEGIRSTMQSLAPNLMSLSSSPAEELDTAINENIGIALVLIEKASMDKSTQDLADQLMQAIAIRRYHKERRADQPFITQNTNPYSLSLPEPLGLKNTGVTPQQFRVYEEFGKNIPNLDVIPFAGLPAHAPPMTQNVGLTQPQQQQAQMPTQILTSEQIRAQQQQQQLQKSRLNQPSQSAQPPGVNVPNPQGGIAAVQSDLEQNQRVLVHLMDILVSQIKENATKNNLAELGDQNQIKTIIFQILTFIAKSAQKDQLALKVSQAVVNSLFATSESPLCREVLSLLLEKLCSLSLVARKDVVWWLVYALDSRKFNVPVIRSLLEVNLIDATELDNVLVTAMKNKMENSTEFAMKLIQNTVLSDDPILMRMDFIKTLEHLASSEDENVKKFIKEFEDTKIMPVRKGTKTTRTEKLYLVFTEWVKLLQRVENNDVITTVFIKQLVEKGVISDTDNLLTFVKSSLELSVSSFKESDPTDEVFIAIDALGSLIIKLLILQGFKDDTRRDYINAIFSVIVLVFAKDHSQEGTTFNERPYFRLFSNILYEWATIRTHNFVRISDSSTRQELIEFDSVFYNTFSGYLHALQPFAFPGFSFAWVTLLSHRMLLPIMLRLPNKIGWEKLMLLIIDLFKFLDQYTSKHAVSDAVSVVYKGTLRVILGISNDMPSFLIENHYELMNNLPPTYFQLKNVILSAIPKNMTVPNPYDVDLNMEDIPACKELPEVFFDPVIDLHSLKKPVDNYLRIPSNSLLRTILSAIYKDTYDIKKGVGYDFLSVDSKLIRAIVLHVGIEAGIEYKRTSSNAVFNTKSSYYTLLFNLIQNGSIEMKYQIILSIVEQLRYPNIHTYWFSFVLMNMFKSDEWNDQKLEVQEIILRNFLKRIIVNKPHTWGVSVFFTQLINNNDINLLDLPFVQSVPEIKLILQQLVKYSKKYTTSEQDDQSATINRRQTPLQSNA 5B2AE15BCF95E009 nacb1_yeast Nascent polypeptide-associated complex subunit beta-1 EGD1 Beta-1-NAC GAL4 DNA-binding enhancer protein 1 BTF3 homolog EGD1 YPL037C protein protein yeast Saccharomyces cerevisiae Baker's yeast MPIDQEKLAKLQKLSANNKVGGTRRKLNKKAGSSAGANKDDTKLQSQLAKLHAVTIDNVAEANFFKDDGKVMHFNKVGVQVAAQHNTSVFYGLPQEKNLQDLFPGIISQLGPEAIQALSQLAAQMEKHEAKAPADAEKKDEAIPELVEGQTFDADVE 7C278A3DC79BF95B ubc4_yeast Ubiquitin-conjugating enzyme E2 4 UBC4 YBR082C YBR0745 Ubiquitin-protein ligase 4 Ubiquitin carrier protein 4 E2 ubiquitin-conjugating enzyme 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAHIYKTDRPKYEATAREWTKKYAV 8E96137D3EB20F80 ubc5_yeast Ubiquitin-conjugating enzyme E2-16 kDa UBC5 Ubiquitin-protein ligase Ubiquitin carrier protein YD9609.13C D4234 YDR059C E2 ubiquitin-conjugating enzyme 5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSKRIAKELSDLGRDPPASCSAGPVGDDLYHWQASIMGPSDSPYAGGVFFLSIHFPTDYPFKPPKVNFTTKIYHPNINSSGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAQIYKTDKAKYEATAKEWTKKYAV not2_yeast General negative regulator of transcription subunit 2 CDC36 DNA19 NOT2 YDL165W cell division cycle protein 36 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEKFGLKALVPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPWAETSRSEVEPRFFTPESFTNIPGVLQSTVTPPCFNSIQNDQQRVALFQDETLFFLFYKHPGTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQRDFLLFYNAIM 7D0B69657F791371 not3_yeast General negative regulator of transcription subunit 3 NOT3 YIL038C protein protein yeast Saccharomyces cerevisiae Baker's yeast MAHRKLQQEVDRVFKKINEGLEIFNSYYERHESCTNNPSQKDKLESDLKREVKKLQRLREQIKSWQSSPDIKDKDSLLDYRRSVEIAMEKYKAVEKASKEKAYSNISLKKSETLDPQERERRDISEYLSQMIDELERQYDSLQVEIDKLLLLNKKKKTSSTTNDEKKEQYKRFQARYRWHQQQMELALRLLANEELDPQDVKNVQDDINYFVESNQDPDFVEDETIYDGLNLQSNEAIAHEVAQYFASQNAEDNNTSDANESLQDISKLSKKEQRKLEREAKKAAKLAAKNATGAAIPVAGPSSTPSPVIPVADASKETERSPSSSPIHNATKPEEAVKTSIKSPRSSADNLLPSLQKSPSSATPETPTNVHTHIHQTPNGITGATTLKPATLPAKPAGELKWAVAASQAVEKDRKVTSASSTISNTSTKTPTTAAATTTSSNANSRIGSALNTPKLSTSSLSLQPDNTGASSSAATAAAVLAAGAAAVHQNNQAFYRNMSSSHHPLVSLATNPKSEHEVATTVNQNGPENTTKKVMEQKEEESPEERNKLQVPTFGVFDDDFESDRDSETEPEEEEQPSTPKYLSLEQREAKTNEIKKEFVSDFETLLLPSGVQEFIMSSELYNSQIESKITYKRSRDMCEISRLVEVPQGVNPPSPLDAFRSTQQWDVMRCSLRDIIIGSERLKEDSSSIYAKILENFRTLEMFSLFYNYYFAITPLEREIAYKILNERDWKVSKDGTMWFLRQGEVKFFNEICEVGDYKIFKLDDWTVIDKINFRLDYSFLQPPVDTASEVRDVSVDNNNVNDQSNVTLEQQKQEISHGKQLLKQLKQGKISV 69B76694FCC6846F not4_yeast General negative regulator of transcription subunit 4 MOT2 CCL1 NOT4 SIG1 SSF1 Modulator of transcription 2 YER068W protein protein yeast Saccharomyces cerevisiae Baker's yeast MMNPHVQENLQAIHNALSNFDTSFLSEDEEDYCPLCIEPMDITDKNFFPCPCGYQICQFCYNNIRQNPELNGRCPACRRKYDDENVRYVTLSPEELKMERAKLARKEKERKHREKERKENEYTNRKHLSGTRVIQKNLVYVVGINPPVPYEEVAPTLKSEKYFGQYGKINKIVVNRKTPHSNNTTSEHYHHHSPGYGVYITFGSKDDAARCIAQVDGTYMDGRLIKAAYGTTKYCSSYLRGLPCPNPNCMFLHEPGEEADSFNKRELHNKQQAQQQSGGTAFTRSGIHNNISTSTAGSNTNLLSENFTGTPSPAAMRAQLHHDSHTNAGTPVLTPAPVPAGSNPWGVTQSATPVTSINLSKNSSSINLPTLNDSLGHHTTPTTENTITSTTTTTNTNATSHSHGSKKKQSLAAEEYKDPYDALGNAVDFLDARLHSLSNYQKRPISIKSNIIDEETYKKYPSLFSWDKIEASKKSDNTLANKLVEILAIKPIDYTASVVQFLQSVNVGVNDNITITDNTKTPTQPIRLQTVSQQIQPPLNVSTPPPGIFGPQHKVPIQQQQMGDTSSRNSSDLLNQLINGRKIIAGN 8847084BD9BF48B7 not5_yeast General negative regulator of transcription subunit 5 NOT5 YP9499.27 YPR072W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQRKLQQDIDKLLKKVKEGIEDFDDIYEKFQSTDPSNSSHREKLESDLKREIKKLQKHRDQIKTWLSKEDVKDKQSVLMTNRRLIENGMERFKSVEKLMKTKQFSKEALTNPDIIKDPKELKKRDQVLFIHDCLDELQKQLEQYEAQENEEQTERHEFHIANLENILKKLQNNEMDPEPVEEFQDDIKYYVENNDDPDFIEYDTIYEDMGCEIQPSSSNNEAPKEGNNQTSLSSIRSSKKQERSPKKKAPQRDVSISDRATTPIAPGVESASQSISSTPTPVSTDTPLHTVKDDSIKFDNSTLGTPTTHVSMKKKESENDSEQQLNFPPDRTDEIRKTIQHDVETNAAFQNPLFNDELKYWLDSKRYLMQPLQEMSPKMVSQLESSLLNCPDSLDADSPCLYTKPLSLPHPTSIFFPNEPIRFVYPYDVPLNLTNNENDTDNKFGKDSKAKSKKDDDIYSRTSLARIFMKFDLDTLFFIFYHYQGSYEQFLAARELFKNRNWLFNKVDRCWYYKEIEKLPPGMGKSEEESWRYFDYKKSWLARRCGNDFVYNEEDFEKL 073137119B3E92BC pop2_yeast Poly(A) ribonuclease POP2 POP2 CAF1 YNR052C N3470 CCR4-associated factor 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MQSMNVQPRVLAVGGEQFFSQRQASEQHQQQNMGPQVYSPKVNRARMFPQGMPVNTINGSVNQEMNNAYLLKQKNEPLLTQQQQQQQQQQQPFNIGTPVSVASLPPGLNVLQQQQQQQQQQQQQQQGVGLNRPLASQLPKHLTNQSMPPIFLPPPNYLFVRDVWKSNLYSEFAVIRQLVSQYNHVSISTEFVGTLARPIGTFRSKVDYHYQTMRANVDFLNPIQLGLSLSDANGNKPDNGPSTWQFNFEFDPKKEIMSTESLELLRKSGINFEKHENLGIDVFEFSQLLMDSGLMMDDSVTWITYHAAYDLGFLINILMNDSMPNNKEDFEWWVHQYMPNFYDLNLVYKIIQEFKNPQLQQSSQQQQQQQYSLTTLADELGLPRFSIFTTTGGQSLLMLLSFCQLSKLSMHKFPNGTDFAKYQGVIYGIDGDQ F3CAF97106A65933 caf40_yeast Protein CAF40 YNL288W N0540 40 kDa CCR4-associated factor CAF40 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSAQKPIYGNGAGVNMGGGGPSTNNPGSMSMPGVPTSMGPGMNQQIPSGGPMLMGNTPNNNNSNENGENNGNNGNNGGNDANATRNNPNMVNNRGAVHALDDPNVYHWICQLTYGPQKEQALLELGRKREQFDDLAVVLWSSFGVMTSLLNEIISVYPMLQPQMLSNNLSNRVCNALVLLQCVASHPETKHLFLQAHIPLFLFPFLNTTSRQRTFEYLRLTSLGVIGALVKNDSQDVITFLLRTDIVPLCLRIMESSSELSKTVAIFILQKILLDDVGLQYICATLERFYAVTNVLKDMVEHLTVSTPPGRLLKHIIRCYLRLSDDLEARRLLKIVLPAKLRDNTFTEVLRDDVGSKRCLAQLLLTLNEETS F3DBABE6AD3C7F3C ccr4_yeast CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 CCR4 Carbon catabolite repressor protein 4 Cytoplasmic deadenylase Glucose-repressible alcohol dehydrogenase transcriptional effector FUN27 YAL021C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNDPSLLGYPNVGPQQQQQQQQQQHAGLLGKGTPNALQQQLHMNQLTGIPPPGLMNNSDVHTSSNNNSRQLLDQLANGNANMLNMNMDNNNNNNNNNNNNNNNGGGSGVMMNASTAAVNSIGMVPTVGTPVNINVNASNPLLHPHLDDPSLLNNPIWKLQLHLAAVSAQSLGQPNIYARQNAMKKYLATQQAQQAQQQAQQQAQQQVPGPFGPGPQAAPPALQPTDFQQSHIAEASKSLVDCTKQALMEMADTLTDSKTAKKQQPTGDSTPSGTATNSAVSTPLTPKIELFANGKDEANQALLQHKKLSQYSIDEDDDIENRMVMPKDSKYDDQLWHALDLSNLQIFNISANIFKYDFLTRLYLNGNSLTELPAEIKNLSNLRVLDLSHNRLTSLPAELGSCFQLKYFYFFDNMVTTLPWEFGNLCNLQFLGVEGNPLEKQFLKILTEKSVTGLIFYLRDNRPEIPLPHERRFIEINTDGEPQREYDSLQQSTEHLATDLAKRTFTVLSYNTLCQHYATPKMYRYTPSWALSWDYRRNKLKEQILSYDSDLLCLQEVESKTFEEYWVPLLDKHGYTGIFHAKARAKTMHSKDSKKVDGCCIFFKRDQFKLITKDAMDFSGAWMKHKKFQRTEDYLNRAMNKDNVALFLKLQHIPSGDTIWAVTTHLHWDPKFNDVKTFQVGVLLDHLETLLKEETSHNFRQDIKKFPVLICGDFNSYINSAVYELINTGRVQIHQEGNGRDFGYMSEKNFSHNLALKSSYNCIGELPFTNFTPSFTDVIDYIWFSTHALRVRGLLGEVDPEYVSKFIGFPNDKFPSDHIPLLARFEFMKTNTGSKKV 5CDB2E8FEDDBEF6F naca_yeast Nascent polypeptide-associated complex subunit alpha EGD2 Alpha-NAC GAL4 DNA-binding enhancer protein 2 YHR193C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAIPENANVTVLNKNEKKARELIGKLGLKQIPGIIRVTFRKKDNQIYAIEKPEVFRSAGGNYVVFGEAKVDNFTQKLAAAQQQAQASGIMPSNEDVATKSPEDIQADMQAAAEGSVNAAAEEDDEEGEVDAGDLNKDDIELVVQQTNVSKNQAIKALKAHNGDLVNAIMSLSK 86CE0C4E6194C286 rl25_yeast 60S ribosomal protein L25 YOL127W RPL25 YL25 RP16L YP42' Large ribosomal subunit protein uL23 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAPSAKATAAKKAVVKGTNGKKALKVRTSATFRLPKTLKLARAPKYASKAVPHYNRLDSYKVIEQPITSETAMKKVEDGNILVFQVSMKANKYQIKKAVKELYEVDVLKVNTLVRPNGTKKAYVRLTADYDALDIANRIGYI uba1_yeast Ubiquitin-activating enzyme E1 1 UBA1 YKL210W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSNNSGLSAAGEIDESLYSRQLYVLGKEAMLKMQTSNVLILGLKGLGVEIAKNVVLAGVKSMTVFDPEPVQLADLSTQFFLTEKDIGQKRGDVTRAKLAELNAYVPVNVLDSLDDVTQLSQFQVVVATDTVSLEDKVKINEFCHSSGIRFISSETRGLFGNTFVDLGDEFTVLDPTGEEPRTGMVSDIEPDGTVTMLDDNRHGLEDGNFVRFSEVEGLDKLNDGTLFKVEVLGPFAFRIGSVKEYGEYKKGGIFTEVKVPRKISFKSLKQQLSNPEFVFSDFAKFDRAAQLHLGFQALHQFAVRHNGELPRTMNDEDANELIKLVTDLSVQQPEVLGEGVDVNEDLIKELSYQARGDIPGVVAFFGGLVAQEVLKACSGKFTPLKQFMYFDSLESLPDPKNFPRNEKTTQPVNSRYDNQIAVFGLDFQKKIANSKVFLVGSGAIGCEMLKNWALLGLGSGSDGYIVVTDNDSIEKSNLNRQFLFRPKDVGKNKSEVAAEAVCAMNPDLKGKINAKIDKVGPETEEIFNDSFWESLDFVTNALDNVDARTYVDRRCVFYRKPLLESGTLGTKGNTQVIIPRLTESYSSSRDPPEKSIPLCTLRSFPNKIDHTIAWAKSLFQGYFTDSAENVNMYLTQPNFVEQTLKQSGDVKGVLESISDSLSSKPHNFEDCIKWARLEFEKKFNHDIKQLLFNFPKDAKTSNGEPFWSGAKRAPTPLEFDIYNNDHFHFVVAGASLRAYNYGIKSDDSNSKPNVDEYKSVIDHMIIPEFTPNANLKIQVNDDDPDPNANAANGSDEIDQLVSSLPDPSTLAGFKLEPVDFEKDDDTNHHIEFITACSNCRAQNYFIETADRQKTKFIAGRIIPAIATTTSLVTGLVNLELYKLIDNKTDIEQYKNGFVNLALPFFGFSEPIASPKGEYNNKKYDKIWDRFDIKGDIKLSDLIEHFEKDEGLEITMLSYGVSLLYASFFPPKKLKERLNLPITQLVKLVTKKDIPAHVSTMILEICADDKEGEDVEVPFITIHL 8910804DF9156661 ubiq_yeast Ubiquitin UBI1 RPL40A YIL148W UBI2 RPL40B YKR094C UBI3 RPS31 YLR167W L9470.14 UBI4 SCD2 YLL039C protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG obsolete protein egd1-not1 Interaction between EGD and Not1 801104 n/a 801107 unspecified role unspecified role prey prey n/a 801108 unspecified role unspecified role bait bait physical association physical association false false false Figure 1a ubc4-egd1 Interaction between Ubc4 and EGD1 801104 n/a 801109 unspecified role unspecified role bait bait n/a 801108 unspecified role unspecified role prey prey physical association physical association false false false Figure 3a ubc5-egd1 Interaction between Ubc5 and Egd1 801104 n/a 801108 unspecified role unspecified role prey prey n/a 801110 unspecified role unspecified role bait bait physical association physical association false false false Figure 3a egd1-egd1 Interaction between Egd1 and Egd1 801104 n/a 801108 unspecified role unspecified role bait bait n/a 801108 unspecified role unspecified role prey prey physical association physical association false false false figure 4c egd1-not2 Interaction between EGD and Not2 801104 n/a 801108 unspecified role unspecified role bait bait n/a 801111 unspecified role unspecified role prey prey physical association physical association false false false Figure 1a egd1-not3 Interaction between EGD and Not3 801104 n/a 801108 unspecified role unspecified role bait bait n/a 801112 unspecified role unspecified role prey prey physical association physical association false false false Figure 1a egd1-not4 Interaction between EGD and Not4 801104 n/a 801108 unspecified role unspecified role bait bait n/a 801113 unspecified role unspecified role prey prey physical association physical association false false false Figure 1a egd1-not5 Interaction between EGD and Not5 801104 n/a 801114 unspecified role unspecified role prey prey n/a 801108 unspecified role unspecified role bait bait physical association physical association false false false Figure 1a egd1-caf1 Interaction between EGD and Caf1 801104 n/a 801108 unspecified role unspecified role bait bait n/a 801115 unspecified role unspecified role prey prey physical association physical association false false false Figure 1a egd1-caf40 Interaction between EGD and Caf40 801104 n/a 801116 unspecified role unspecified role prey prey n/a 801108 unspecified role unspecified role bait bait physical association physical association false false false Figure 1a egd1-ccr4 Interaction between EGD and Ccr4 801104 n/a 801117 unspecified role unspecified role prey prey n/a 801108 unspecified role unspecified role bait bait physical association physical association false false false Figure 1a ubc4-egd2 Interaction between Ubc4 and EGD2 801104 n/a 801109 unspecified role unspecified role bait bait n/a 801118 unspecified role unspecified role prey prey physical association physical association false false false Figure 3a ubc4-not4 Interaction between Ubc4 and Not4 801104 n/a 801113 unspecified role unspecified role prey prey n/a 801109 unspecified role unspecified role bait bait physical association physical association false false false Figure 3a egd1-egd2 Interaction between Egd1 and Egd2 801104 n/a 801118 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 801108 unspecified role unspecified role bait bait physical association physical association false false false figures 1A and 4c ubc5-egd2 Interaction between Ubc5 and Egd2 801104 n/a 801118 unspecified role unspecified role prey prey n/a 801110 unspecified role unspecified role bait bait physical association physical association false false false Figure 3a ubc5-not4 Interaction between Ubc5 and Not4 801104 n/a 801110 unspecified role unspecified role bait bait n/a 801113 unspecified role unspecified role prey prey physical association physical association false false false Figure 3a egd-ccr4-not Interaction between EGD and Ccr4-Not complexes 801105 n/a 801118 unspecified role unspecified role prey prey n/a 801119 unspecified role unspecified role prey prey n/a 801108 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 801113 unspecified role unspecified role prey prey n/a 801114 unspecified role unspecified role prey prey association association false false false Figure 1b caf40-egd2 Interaction between Caf40 and Caf40, Not4, Egd2 801105 n/a 801116 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 801118 unspecified role unspecified role prey prey n/a 801113 unspecified role unspecified role prey prey association association false false false Figure 1C not4-egd Interaction between Not4 and EGD complex 801106 n/a 801113 enzyme enzyme neutral component neutral component yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 801118 enzyme target enzyme target substrate substrate neutral component neutral component yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 801108 unspecified role unspecified role ancillary ancillary yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 801120 unspecified role unspecified role ancillary ancillary n/a 801109 unspecified role unspecified role ancillary ancillary yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 801121 unspecified role unspecified role ancillary ancillary yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ubiquitination ubiquitination reaction ubiquitination false false false Figure 3b