UniProt UniProt http://www.uniprot.org araki-2006-1 The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast fluorescence fluorescence technology fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence Araki Y., Lau CK., Maekawa H., Jaspersen SL., Giddings TH., Schiebel E., Winey M. Mark.Winey@colorado.edu Mol. Biol. Cell (1059-1524) 2006 Only protein-protein interactions imex curation araki-2006-2 The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Araki Y., Lau CK., Maekawa H., Jaspersen SL., Giddings TH., Schiebel E., Winey M. Mark.Winey@colorado.edu Mol. Biol. Cell (1059-1524) 2006 Only protein-protein interactions imex curation araki-2006-3 The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap anti tag western anti tag western blot anti tag western Araki Y., Lau CK., Maekawa H., Jaspersen SL., Giddings TH., Schiebel E., Winey M. Mark.Winey@colorado.edu Mol. Biol. Cell (1059-1524) 2006 Only protein-protein interactions imex curation spc42_yeast Spindle pole body component SPC42 SPC42 YKL255 YKL042W protein protein yeast Saccharomyces cerevisiae Baker's yeast MNGSPTPKRYSSKSSRLYDDYYNIPYQYSNPTPMNRDYNDVGSRINADKLVPEEYKRNTEFINKAVQQNKELNFKLREKQNEIFELKKIAETLRSKLEKYVDITKKLEDQNLNLQIKISDLEKKLSDANSTFKEMRFPKVKDPMVDDDPVSENYDQINVPKHRAPDATGNPRTTNKVSNTSDQDSRLKAIERTLSVLTNYVMRSEDGNNDRMSPLPSPLNTILPINNRLNFQEPKRYNPTVKVNPSDDDIMMYESAELKRVEEEIEELKRKILVRKKHDLRKLSLNNQLQELQSMMDGDDNIKLDNVSKHNHATHRHSSQSSRDYSPSSDACLECSNDLYEKNRVKPENNMSETFATPTPNNR nbp1_yeast NAP1-binding protein NBP1 L9122.6 YLR457C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLKSVQGLWKDFFGIRDDGRKREYGSLDEVRKRSALRSRRKQMRPTGKSVLKRPRKVTDRKTEEKIRTNRRKTPKRRLTKIFQTIRDVFSNDNENMSKMQNVCGDMTRILKKRSQGRPSYMDTDTAKSRILRSDAFKRKISELKYNKQRISELRSGSSDGSSGKDRNQSLYLDREILLQRQIKKRDEKIKALESKLQSLQEALNYSNEKYRILEDLLDSSNIHPSYTKSRRTMSNLARENDEIKPLKIDLSPSPIRRTNSLFTSSPMKTYNRDGNIPEMQPLQENISPACPTPPYRSRETEKEDETLSPISVDFSSYLS ndc1_yeast Nucleoporin NDC1 YML031W NDC1 Nuclear pore protein NDC1 Nuclear division cycle protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MIQTPRELLNPRYTYHTIFSDVCKTRFNHLVTRLFFICSIIQTVVISLLALPHSPLWELALAFIPNILALNLVSLLIIVTRKNYMHVKNFGFANSLTFILGQLLSVKFLVYQGVYSMGSILLSFVLGVVFGRGGSGWKPYYKLFIWLVVPTIYNLQHHVTDADKLSFNCENFFQAPQDYVLERVKRIMEKSVILSVISMFVLPIFTTVFFSRQKSGLFDSFTNGVLAVTNLLIISCIIFITFEFINIAFDAHMSIGCLHKGKLISNLSSTPMETLLSGLSADKPFTRLTAYQELAYRATSLDPSLRAPIYHSKFRSSSGNTWSLILNECLKTIQINNEKVVQYLRSVQDLGGSATARHKKKVENLDYMYENGKLTSANERLFGNRPSMMAPLRDNGLLDESPNRLRVRTDDSVLLNRGNKKRHRSSYYDNDLDETTQTFNGSIFTHETTFMTAMRLMLKKLKNSIMSFIFPSYAERQSSDESDNYRLLPNGSNKAQISIIDIWSISKKRQAEKLVPLPICHANSVVALTGLLIRSKTEDPKGGIIASVGDILKTLERSICALGEFADWDPESMAYTAFQTQRTAQDRVQQDSEDEDSMKDTTDMISVLYQLSTSAFMEIVLEYNVALNDVYLDADVAKLANWFLEVYASGNPNAT bbp1_yeast Spindle pole component BBP1 BBP1 YPL255W P0745 BFR1-binding protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNQEDNTGGGGIFGLFKWTKDALFGTDISPSMKYKDQEERRDRSRYAQDDTNFSMKFGNDSNRRSTNLSRSNSWSGLDSTLHRKYELLPEYNENGFNSIVNGDHHSKERIRSLRSPAPIVPREPLRNEPTDTFGHRLHTKRRTINELSNSQIPFIPPQEDDPLLSKLFNKDGVNEVRRSPYKLSVKDIPGKFPSPLTKRDEIDNYYVRDEDACHKNREYKKAYFDLFAQMDLNSRDLEDLCEDVREQREQFHRNEQTYKQAYEEMRAELVNELKKSKTLFENYYSLGQKYKSLKKVLDQTISHEAELATSRERLYQEEDLKNFEIQTLKQRLSDLELKYTNLQIEKDMQRDNYESEIHDLLLQLSLRNNERKDTSAGSNIFSTGQ 3A1A827E7664E4E0 mps2_yeast Monopolar spindle protein 2 YGL075C MPS2 MMC1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNGAFDAIFEYAWGQIDKPISGDFIYGKDLPKLIEIIENIFQKAQKSGSYELRLPLFSEINKDLFRTFSNTKTFFKIHKEEFDDIFFNLVNHPLREILENAFIGVDSIPSDFIVSMNLNSPSKFLVENKNKNTEGAGISTPRKKLTESPIKLLSRNNIGKALEVQVEELKRELTAKQSLLQENERQVSELKIRLETYQEKYASIQQRFSDLQKARQVEDNQNSSRTSDPGSPLVTGIDQKAILEEFRRRLQRQTDTISFLKDQIRRERGLNCSNDKVSHSKRKHATTDGDGTFKNFISAVPSNIWVKATIRIIVCFALLAGVLPYIRKYVYAHDTPSQNSRLQLSWWENSGILSKIVWFFEDQTDLETEYRSNANVDDAYSRVFGI nbp1-spc42-1 787010 n/a 787013 unspecified role unspecified role neutral component neutral component c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 787014 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 4A nbp1-ndc1-1 787010 n/a 787015 unspecified role unspecified role neutral component neutral component c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 787014 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 4B nbp1-spc42-2 787010 n/a 787014 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 787013 unspecified role unspecified role neutral component neutral component eqfp-tag c-terminus tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 6B, 9 Co-localization requires MPS2. bbp1-spc42-1 787010 n/a 787016 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 787013 unspecified role unspecified role neutral component neutral component eqfp-tag c-terminus tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 8A mps2-spc42-1 787010 n/a 787017 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 787013 unspecified role unspecified role neutral component neutral component eqfp-tag c-terminus tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 8B ndc1-nbp1-1 787011 n/a 787015 unspecified role unspecified role bait bait n/a 787014 unspecified role unspecified role prey prey physical association physical association false false false Fig 5A nbp1-mps2-1 787011 n/a 787017 unspecified role unspecified role prey prey n/a 787014 unspecified role unspecified role bait bait physical association physical association false false false Fig 5A nbp1-bbp1-1 787012 n/a 787016 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 787014 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 5B nbp1-mps2-2 787012 n/a 787014 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 787017 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 5B