IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chen-2006f-1 The PII signal transduction protein of Arabidopsis thaliana forms an arginine-regulated complex with plastid N-acetyl glutamate kinase. in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Chen YM., Ferrar TS., Lohmeier-Vogel EM., Lohmeir-Vogel E., Morrice N., Mizuno Y., Berenger B., Ng KK., Muench DG., Moorhead GB. moorhead@ucalgary.ca J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation glnb_arath Nitrogen regulatory protein P-II homolog GLB1 GLNB1 At4g01900 Protein PII-like T7B11.16 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAASMTKPISITSLGFYSDRKNIAFSDCISICSGFRHSRPSCLDLVTKSPSNNSRVLPVVSAQISSDYIPDSKFYKVEAIVRPWRIQQVSSALLKIGIRGVTVSDVRGFGAQGGSTERHGGSEFSEDKFVAKVKMEIVVKKDQVESVINTIIEGARTGEIGDGKIFVLPVSDVIRVRTGERGEKAEKMTGDMLSPS nagk_arath Acetylglutamate kinase, chloroplastic NAGK N-acetyl-L-glutamate 5-phosphotransferase NAG kinase T8H10.160 At3g57560 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATVTSNASPKSFSFTVSNPFKTLIPNKSPSLCYPTRNKNHHRLGFSIKATVSTPPSIATGNAPSPDYRVEILSESLPFIQKFRGKTIVVKYGGAAMTSPELKSSVVSDLVLLACVGLRPILVHGGGPDINRYLKQLNIPAEFRDGLRVTDATTMEIVSMVLVGKVNKNLVSLINAAGATAVGLSGHDGRLLTARPVPNSAQLGFVGEVARVDPSVLRPLVDYGYIPVIASVAADDSGQAYNINADTVAGELAAALGAEKLILLTDVAGILENKEDPSSLIKEIDIKGVKKMIEDGKVAGGMIPKVKCCIRSLAQGVKTASIIDGRRQHSLLHEIMSDEGAGTMITG copa1_arath Coatomer subunit alpha-1 Alpha-coat protein 1 F8K4.21 At1g62020 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLTKFETKSNRVKGLSFHPKRPWILASLHSGVIQLWDYRMGTLIDRFDEHEGPVRGVHFHNSQPLFVSGGDDYKIKVWNYKNHRCLFTLLGHLDYIRTVQFHHEYPWIVSASDDQTIRIWNWQSRTCVSVLTGHNHYVMCASFHPKEDLVVSASLDQTVRVWDIGALRKKTVSPADDIMRLTQMNSDLFGGVDAIVKYVLEGHDRGVNWAAFHPTLPLIVSGADDRQVKLWRMNETKAWEVDTLRGHMNNVSSVMFHAKQDIIVSNSEDKSIRVWDATKRTGLQTFRREHDRFWILAVHPEMNLLAAGHDSGMIVFKLERERPAFALSGDSLFYAKDRFLRYYEYSTQRDSQVIPIRRPGTPSLNQSPRTLSYSPTENAVLICSDLDGGSYELYIIPKDSVGRSDVVQDAKRGTGGSAVFIARNRFAVLEKSTSQVLVKNLKNEVVKKSPLPIPTDAIFYAGTGNLLCRSEDKVVIFDLQQRLVLGELQTPFVRYVVWSSDMESVALLSKHTIIIASKKLVLQCTLHETIRVKSGAWDDNGVFIYTTLNHIKYCLPNGDSGIIRTLDVPIYITKVSGNTIFCLDRDGKNKAITINATEYIFKLSLLRKRYDHVMSMIKNSQLCGQAMIAYLQQKGFPEVALHFVEDERIRFNLALESGNISVAVASATQINEKDHWYRLGVEALRQGNSGIVEFAYQQTKNFERLSFLYLITGNLDKLSKLMKIAEVKNNVMGQFHNALYLGDVKERVKILENAGHLPLAYITASVHGLNDIAERLATELGDNVPSLPEGKTPSLLMPPTPIMCGGDWPLLRVMKGIFEGGLESADRGGTVDEEDVEGDWGEELDINVDGMENRDIEDILAAAEAGEEENDEEGGWGLEDLVLPPELDTPKASANARSSVFVTPPQGMPVSQSWSQKSSLAAEQAAAGSFDTAMRLLHRQLGIKNFTPLKSMFLDLFNGSHSYLRAFSSCPVVPLAIERGWSESSSPNVRSPPALVYDFSQLDEKLKSGYKATTTGKFTEALRLFLSILHTIPLVVVETRREVDEVKELIVIVKEYVLGLQMELKRREMKDDPVRQQELAAYFTHCNLQTPHLRLALLSAMGVCYKAKNLATASNFARRLLETSPVDSQAKMARQVVQAAERNMTDETKLNYDFRNPFVVCGSTYVPIYRGQKDVSCPYCTARFVPNQEGNICTVCDLAVIGADASGLLCSPSQVR copb2_arath Coatomer subunit beta-2 Beta-coat protein 2 F3L17.60 At4g31490 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDKSSTMLVHYDKGTPAVANEIKEALEGNDVEAKVDAMKKAIMLLLNGETIPQLFITIIRYVLPSEDHTIQKLLLLYLELIEKTDSKGKVLPEMILICQNLRNNLQHPNEYIRGVTLRFLCRMKETEIVEPLTPSVLQNLEHRHPFVRRNAILAIMSIYKLPHGDQLFVDAPEMIEKVLSTEQDPSAKRNAFLMLFTCAEERAVNYLLSNVDKVSDWNESLQMVVLELIRSVCKTKPAEKGKYIKIIISLLSATSSAVIYECAGTLVSLSSAPTAIRAAANTYCQLLLSQSDNNVKLILLDRLYELKTLHRDIMVELIIDVLRALSSPNLDIRRKTLDIALDLITHHNINEVVQMLKKEVVKTQSGELEKNGEYRQMLIQAIHACAVKFPEVASTVVHLLMDFLGDSNVASALDVVVFVREIIETNPKLRVSIITRLLDTFYQIRAGKVCPCALWIIGEYCLSLSEVESGISTITQCLGELPFYSVSEESEPTETSKKIQPTSSAMVSSRKPVILADGTYATQSAASETTFSSPTVVQGSLTSGNLRALLLTGDFFLGAVVACTLTKLVLRLEEVQSSKTEVNKTVSQALLIMVSILQLGQSPVSPHPIDNDSYERIMLCIKLLCHRNVEMKKIWLESCRQSFVKMISEKQLREMEELKAKTQTTHAQPDDLIDFFHLKSRKGMSQLELEDQVQDDLKRATGEFTKDENDANKLNRILQLTGFSDPVYAEAYVTVHHYDIALEVTVINRTKETLQNLCLELATMGDLKLVERPQNYSLAPERSMQIKANIKVSSTETGVIFGNIVYETSNVMERNVVVLNDIHIDIMDYISPAVCSEVAFRTMWAEFEWENKVAVNTTIQNEREFLDHIIKSTNMKCLTAPSAIAGECGFLAANLYAKSVFGEDALVNLSIEKQTDGTLSGYIRIRSKTQGIALSLGDKITLKQKGGS copg_arath Coatomer subunit gamma Gamma-coat protein T4L20.30 At4g34450 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAQPLVKKDDDHDDELEYSPFMGIEKGAVLQEARVFNDPQVDPRRCSQVITKLLYLLNQGESFTKVEATEVFFSVTKLFQSKDTGLRRMVYLIIKELSPSSDEVIIVTSSLMKDMNSKIDMYRANAIRVLCRIIDGTLLTQIERYLKQAIVDKNPVVSSAALVSGLHLLKTNPEIVKRWSNEVQEGIQSRSALVQFHALALLHQIRQNDRLAVSKLVGSLTRGSVRSPLAQCLLIRYTSQVIRDMANHGQSGERPFYEFLESCLRHKAEMVILEAARAITELDGVTSRELTPAITVLQLFLSSPRPVLRFAAVRTLNKVAMTHPMAVTNCNIDMESLISDQNRSIATLAITTLLKTGNESSVERLMKQITNFMSDIADEFKIVVVDAIRSLCVKFPLKYRSLMTFLSNILREEGGFEYKRAIVDSIVTIIRDIPDAKESGLLHLCEFIEDCEFTYLSTQILHFLGIEGPNTSDPSKYIRYIYNRVHLENATVRAAAVSTLAKFGFMVESLKPRITVLLKRCIYDSDDEVRDRATLYLSVLGGDGTVDTDKESKDFLFGSLEVPLVNMETSLKNYEPSEEAFDINSVPKEVKSQPLAEKKAQGKKPTGLGAPPAAPASGFDGYERLLSSIPEFAAFGKLFKSSLPVELTEAETEYAVNVVKHIFDSHVVFQYNCTNTIPEQLLERVNVIVDASEAEEFSEVTSKALNSLPYDSPGQAFVVFEKPAGVPAVGKFSNTLTFVVKEVDPSTGEAEDDGVEDEYQLEDLEVVAGDYMVKVGVSNFRNAWESMDEEDERVDEYGLGQRESLGEAVKAVMDLLGMQTCEGTETIPLNARSHTCLLSGVYIGNVKVLVRAQFGMDSSKDIAMKLTVRAEDVSVAEAIHEIVASG glb1-at3g57560-2 857938 n/a 857939 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 857940 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false Figure 1 Table 1 glb1-copa1 857938 n/a 857939 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 857941 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false Table 1 glb1-copb2 857938 n/a 857942 unspecified role unspecified role prey prey n/a 857939 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Table 1 glb1-copq 857938 n/a 857943 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress n/a 857939 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Table 1