UniProt UniProt http://www.uniprot.org iwase-2006-1 Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu Mol. Biol. Cell (1059-1524) 2006 Only protein-protein interactions imex curation iwase-2006-2 Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu Mol. Biol. Cell (1059-1524) 2006 Only protein-protein interactions imex curation iwase-2006-3 Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu Mol. Biol. Cell (1059-1524) 2006 Only protein-protein interactions imex curation iwase-2006-4 Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu Mol. Biol. Cell (1059-1524) 2006 Only protein-protein interactions imex curation iwase-2006-5 Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast fluorescence fluorescence technology fluorescence immunostaining immunostaining Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu Mol. Biol. Cell (1059-1524) 2006 Only protein-protein interactions imex curation gic1_yeast GTPase-interacting component 1 YHR061C GIC1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTEGKRLQQMELPQMKSIWIDEDQEMEKLYGFQVRQRFMNGPSTDSDEDADEDLGIVLVDSKKLALPNKNNIKLPPLPNYMTINPNINSNHKSLTNKKKNFLGMFKKKDLLSRRHGSAKTAKQSSISTPFDFHHISHANGKREDNPLESHEEKHDVESLVKFTSLAPQPRPDSNVSSKYSNVVMNDSSRIVSSSTIATTMDSHHDGNETNNTPNGNKQLDSPTDLEMTLEDLRNYTFPSVLGDSVSEKTNPSSPSVSSFSGKFKPRELSALHTPELGNCFNVDQSLNSPGNRISVDDVLKFYYQCSETSTPRNT cdc11_yeast Cell division control protein 11 CDC11 PSL9 YJR076C J1833 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGIIDASSALRKRKHLKRGITFTVMIVGQSGSGRSTFINTLCGQQVVDTSTTILLPTDTSTEIDLQLREETVELEDDEGVKIQLNIIDTPGFGDSLDNSPSFEIISDYIRHQYDEILLEESRVRRNPRFKDGRVHCCLYLINPTGHGLKEIDVEFIRQLGSLVNIIPVISKSDSLTRDELKLNKKLIMEDIDRWNLPIYNFPFDEDEISDEDYETNMYLRTLLPFAIIGSNEVYEMGGDVGTIRGRKYPWGILDVEDSSISDFVILRNALLISHLHDLKNYTHEILYERYRTEALSGESVAAESIRPNLTKLNGSSSSSTTTRRNTNPFKQSNNINNDVLNPASDMHGQSTGENNETYMTREEQIRLEEERLKAFEERVQQELLLKRQELLQREKELREIEARLEKEAKIKQEE 5CFFD4CD1F6C57D4 cdc12_yeast Cell division control protein 12 CDC12 CLA10 PSL7 YHR107C Septin protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAATATAAPVPPPVGISNLPNQRYKIVNEEGGTFTVMLCGESGLGKTTFINTLFQTVLKRADGQQHRQEPIRKTVEIDITRALLEEKHFELRVNVIDTPGFGDNVNNNKAWQPLVDFIDDQHDSYMRQEQQPYRTKKFDLRVHAVLYFIRPTGHGLKPIDIETMKRLSTRANLIPVIAKADTLTAQELQQFKSRIRQVIEAQEIRIFTPPLDADSKEDAKSGSNPDSAAVEHARQLIEAMPFAIVGSEKKFDNGQGTQVVARKYPWGLVEIENDSHCDFRKLRALLLRTYLLDLISTTQEMHYETYRRLRLEGHENTGEGNEDFTLPAIAPARKLSHNPRYKEEENALKKYFTDQVKAEEQRFRQWEQNIVNERIRLNGDLEEIQGKVKKLEEQVKSLQVKKSHLK A72DC393A6DBF924 gic2_yeast GTPase-interacting component 2 D9740.18 YDR309C GIC2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSASITNTGNETMNLPQMRSIWLDEDEEAEKLYGLQAQQFMGSDDEENLGITFINSDKPVLSNKKNIELPPLSPNSHPSCHHRRSNSNSAKSKESSSSSSSANKTNHKKVFLKLNLLKKKLLGAQPDIRGKGISTPFDFQHISHADTRNGFQDEQLQEPSSLSTEIKDDYTSSSSKRDSKSLNKAFVTERIPANRESKLISRSHENKTSRLSVARSISVTSSNYSKNTQGNNHSINGRVVSTSTMATSIFEYSPNASPKQFKNKSHALGHRYTNSTDSSESSLDFLKNYNFPTLLEDKPILDFLPRSQRSSAYRSLLETPNSNKDSAKAFFPSRQSPLPKRRNSIATPSPQSKFSYSDSPVNHRKSFDDVLYSFNQLEPLQT D1BE002BCD48EB03 cdc3_yeast Cell division control protein 3 CDC3 L8543.7 YLR314C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLKEEQVSIKQDPEQEERQHDQFNDVQIKQESQDHDGVDSQYTNGTQNDDSERFEAAESDVKVEPGLGMGITSSQSEKGQVLPDQPEIKFIRRQINGYVGFANLPKQWHRRSIKNGFSFNLLCVGPDGIGKTTLMKTLFNNDDIEANLVKDYEEELANDQEEEEGQGEGHENQSQEQRHKVKIKSYESVIEENGVKLNLNVIDTEGFGDFLNNDQKSWDPIIKEIDSRFDQYLDAENKINRHSINDKRIHACLYFIEPTGHYLKPLDLKFMQSVYEKCNLIPVIAKSDILTDEEILSFKKTIMNQLIQSNIELFKPPIYSNDDAENSHLSERLFSSLPYAVIGSNDIVENYSGNQVRGRSYPWGVIEVDNDNHSDFNLLKNLLIKQFMEELKERTSKILYENYRSSKLAKLGIKQDNSVFKEFDPISKQQEEKTLHEAKLAKLEIEMKTVFQQKVSEKEKKLQKSETELFARHKEMKEKLTKQLKALEDKKKQLELSINSASPNVNHSPVPTKKKGFLR DCCD4C8C16AA5E25 gic1-cdc11-1 786758 n/a 786763 unspecified role unspecified role bait bait n/a 786764 unspecified role unspecified role prey prey physical association physical association false false false Fig 8A gic1-cdc12-2 786758 n/a 786763 unspecified role unspecified role bait bait n/a 786765 unspecified role unspecified role prey prey physical association physical association false false false Fig 8A gic2-cdc12-2 786758 n/a 786765 unspecified role unspecified role prey prey n/a 786766 unspecified role unspecified role bait bait physical association physical association false false false Fig 8A gic2-cdc11-1 786758 n/a 786766 unspecified role unspecified role bait bait n/a 786764 unspecified role unspecified role prey prey physical association physical association false false false Fig 8A gic1-cdc12-3 786759 n/a 786763 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) n/a 786765 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 8B gic2-cdc12-3 786759 n/a 786766 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) n/a 786765 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 8B gic1-cdc3-1 786759 n/a 786767 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 786763 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 8B gic2-cdc3-1 786759 n/a 786767 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 786766 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 8B gic1-cdc12-4 786760 n/a 786765 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 786763 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 8B gic2-cdc12-4 786760 n/a 786766 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) n/a 786765 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 8B gic1-cdc3-2 786760 n/a 786763 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) n/a 786767 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 8B gic2-cdc3-2 786760 n/a 786766 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) n/a 786767 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 8B cdc11-cdc3-1 786761 n/a 786763 unspecified role unspecified role prey prey c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 786767 unspecified role unspecified role prey prey n/a 786765 unspecified role unspecified role prey prey n/a 786764 unspecified role unspecified role bait bait physical association physical association false false false Fig 8C, 8D cdc11-gic2-1 786761 n/a 786764 unspecified role unspecified role bait bait n/a 786766 unspecified role unspecified role prey prey c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 8D gic2-cdc3-3 786762 n/a 786767 unspecified role unspecified role neutral component neutral component n/a 786766 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig S1