UniProt UniProt http://www.uniprot.org vanacova-2005-3 A new yeast poly(A) polymerase complex involved in RNA quality control. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap western blot western blot Immuno blot Immuno blot Vanacova S., Wolf J., Martin G., Blank D., Dettwiler S., Friedlein A., Langen H., Keith G., Keller W. walter.keller@unibas.ch PLoS Biol. (1544-9173) 2005 Only protein-protein interactions imex curation vanacova-2005-2 A new yeast poly(A) polymerase complex involved in RNA quality control. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Vanacova S., Wolf J., Martin G., Blank D., Dettwiler S., Friedlein A., Langen H., Keith G., Keller W. walter.keller@unibas.ch PLoS Biol. (1544-9173) 2005 Only protein-protein interactions imex curation vanacova-2005-1 A new yeast poly(A) polymerase complex involved in RNA quality control. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down ms participant Identification by mass spectrometry ms participant Vanacova S., Wolf J., Martin G., Blank D., Dettwiler S., Friedlein A., Langen H., Keith G., Keller W. walter.keller@unibas.ch PLoS Biol. (1544-9173) 2005 Only protein-protein interactions imex curation air1_yeast Protein AIR1 Arginine methyltransferase-interacting RING finger protein 1 AIR1 YIL079C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTLLSEVESIDTLPYVKDTTPTGSDSSSFNKLLAPSIEDVDANPEELRTLRGQGRYFGITDYDSNGAIMEAEPKCNNCSQRGHLKRNCPHVICTYCGFMDDHYSQHCPKAIICTNCNANGHYKSQCPHKWKKVFCTLCNSKRHSRERCPSIWRSYLLKTKDANQGDFDFQTVFCYNCGNAGHFGDDCAERRSSRVPNTDGSAFCGDNLATKFKQHYFNQLKDYKREASQRQHFDNEHEFNLLDYEYNDDAYDLPGSRTYRDKMKWKGKVQSTRNKNSSNNRYESSNNRKKKSPFSAQNYKVTKNKRVQTHPLDFPRSSQNNRTNDYSSQFSYNRDDFPKGPKNKRGRSSSNKSQRNGRY 3CA920FA1204EC9C mtr4_yeast ATP-dependent RNA helicase DOB1 MTR4 DOB1 YJL050W J1158 mRNA transport regulator MTR4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSTDLFDVFEETPVELPTDSNGEKNADTNVGDTPDHTQDKKHGLEEEKEEHEENNSENKKIKSNKSKTEDKNKKVVVPVLADSFEQEASREVDASKGLTNSETLQVEQDGKVRLSHQVRHQVALPPNYDYTPIAEHKRVNEARTYPFTLDPFQDTAISCIDRGESVLVSAHTSAGKTVVAEYAIAQSLKNKQRVIYTSPIKALSNQKYRELLAEFGDVGLMTGDITINPDAGCLVMTTEILRSMLYRGSEVMREVAWVIFDEVHYMRDKERGVVWEETIILLPDKVRYVFLSATIPNAMEFAEWICKIHSQPCHIVYTNFRPTPLQHYLFPAHGDGIYLVVDEKSTFREENFQKAMASISNQIGDDPNSTDSRGKKGQTYKGGSAKGDAKGDIYKIVKMIWKKKYNPVIVFSFSKRDCEELALKMSKLDFNSDDEKEALTKIFNNAIALLPETDRELPQIKHILPLLRRGIGIHHSGLLPILKEVIEILFQEGFLKVLFATETFSIGLNMPAKTVVFTSVRKWDGQQFRWVSGGEYIQMSGRAGRRGLDDRGIVIMMIDEKMEPQVAKGMVKGQADRLDSAFHLGYNMILNLMRVEGISPEFMLEHSFFQFQNVISVPVMEKKLAELKKDFDGIEVEDEENVKEYHEIEQAIKGYREDVRQVVTHPANALSFLQPGRLVEISVNGKDNYGWGAVVDFAKRINKRNPSAVYTDHESYIVNVVVNTMYIDSPVNLLKPFNPTLPEGIRPAEEGEKSICAVIPITLDSIKSIGNLRLYMPKDIRASGQKETVGKSLREVNRRFPDGIPVLDPVKNMKIEDEDFLKLMKKIDVLNTKLSSNPLTNSMRLEELYGKYSRKHDLHEDMKQLKRKISESQAVIQLDDLRRRKRVLRRLGFCTPNDIIELKGRVACEISSGDELLLTELIFNGNFNELKPEQAAALLSCFAFQERCKEAPRLKPELAEPLKAMREIAAKIAKIMKDSKIEVVEKDYVESFRHELMEVVYEWCRGATFTQICKMTDVYEGSLIRMFKRLEELVKELVDVANTIGNSSLKEKMEAVLKLIHRDIVSAGSLYL 1FA9EAADC546F669 pap2_yeast Poly(A) RNA polymerase protein 2 TRF4 HRC584 O0716 YOL115W PAP2 DNA polymerase sigma DNA polymerase kappa Topoisomerase 1-related protein TRF4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGAKSVTASSSKKIKNRHNGKVKKSKKIKKVRKPQKSISLNDENEVEILPSRNEQETNKLPKDHVTADGILVLEHKSDDDEGFDVYDGHFDNPTDIPSTTEESKTPSLAVHGDEKDLANNDDFISLSASSEDEQAEQEEEREKQELEIKKEKQKEILNTDYPWILNHDHSKQKEISDWLTFEIKDFVAYISPSREEIEIRNQTISTIREAVKQLWPDADLHVFGSYSTDLYLPGSDIDCVVTSELGGKESRNNLYSLASHLKKKNLATEVEVVAKARVPIIKFVEPHSGIHIDVSFERTNGIEAAKLIREWLDDTPGLRELVLIVKQFLHARRLNNVHTGGLGGFSIICLVFSFLHMHPRIITNEIDPKDNLGVLLIEFFELYGKNFGYDDVALGSSDGYPVYFPKSTWSAIQPIKNPFSLAIQDPGDESNNISRGSFNIRDIKKAFAGAFDLLTNRCFELHSATFKDRLGKSILGNVIKYRGKARDFKDERGLVLNKAIIENENYHKKRSRIIHDEDFAEDTVTSTATATTTDDDYEITNPPAKKAKIEEKPESEPAKRNSGETYITVSSEDDDEDGYNPYTL 8A58B29E4BFDC022 air2_yeast Protein AIR2 Arginine methyltransferase-interacting RING finger protein 2 AIR2 YDL175C protein protein yeast Saccharomyces cerevisiae Baker's yeast MEKNTAPFVVDTAPTTPPDKLVAPSIEEVNSNPNELRALRGQGRYFGVSDDDKDAIKEAAPKCNNCSQRGHLKKDCPHIICSYCGATDDHYSRHCPKAIQCSKCDEVGHYRSQCPHKWKKVQCTLCKSKKHSKERCPSIWRAYILVDDNEKAKPKVLPFHTIYCYNCGGKGHFGDDCKEKRSSRVPNEDGSAFTGSNLSVELKQEYYRHMNRNSDENEDYQFSESIYDEDPLPRPSHKRHSQNDHSHSGRNKRRASNFHPPPYQKSNVIQPTIRGETLSLNNNISKNSRYQNTKVNVSSISENMYGSRYNPSTYVDNNSISNSSNYRNYNSYQPYRSGTLGKRR 5915C97FA39D6AFC trf5_yeast Poly(A) RNA polymerase protein 1 TRF5 YNL299W N0440 Topoisomerase 1-related protein TRF5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTRLKAKYSPTKGKRKEDKHTKRMRKSSFTRTQKMLEVFNDNRSHFNKYESLAIDVEDDDTFGNLVLMENDKSDVDIPVIEEVTSSEDEQRAESSKRNNSLEDNQDFIAFSDSSEDETEQIKEDDDERSSFLLTDEHEVSKLTSQQSLNTESACNVEYPWIRNHCHSKQRRIADWLTSEIKDFVHYISPSKNEIKCRNRTIDKLRRAVKELWSDADLHVFGSFATDLYLPGSDIDCVVNSRNRDKEDRNYIYELARHLKNKGLAIRMEVIVKTRVPIIKFIEPQSQLHIDVSFERTNGLEAAKLIREWLRDSPGLRELVLIIKQFLHSRRLNNVHTGGLGGFTVICLVYSFLNMHPRIKSNDIDVLDNLGVLLIDFFELYGKNFGYDDVAISISDGYASYIPKSCWRTLEPSRSKFSLAIQDPGDPNNNISRGSFNMKDIKKAFAGAFELLVNKCWELNSATFKDRVGKSILGNVIKYRGQKRDFNDERDLVQNKAIIENERYHKRRTRIVQEDLFINDTEDLPVEEIYKLDEPAKKKQKAKKDKREGEIKKSAIPSPPPDFGVSRSKLKRKVKKTDQGSLLHQNNLSIDDLMGLSENDQESDQDQKGRDTPSGQDEKSPLETKTVDAQTRRDYWLSKGQAL rrp6_yeast Exosome complex exonuclease RRP6 RRP6 UNC733 Ribosomal RNA-processing protein 6 YOR001W protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSENPDVLLSRVINVVRAASSLASQDVDFYKNLDRGFSKDLKSKADKLADMANEIILSIDEHHESFELKEEDISDLWNNFGNIMDNLLEMSDHSLDKLNCAINSKSRGSDLQYLGEFSGKNFSPTKRVEKPQLKFKSPIDNSESHPFIPLLKEKPNALKPLSESLRLVDDDENNPSHYPHPYEYEIDHQEYSPEILQIREEIPSKSWDDSVPIWVDTSTELESMLEDLKNTKEIAVDLEHHDYRSYYGIVCLMQISTRERDYLVDTLKLRENLHILNEVFTNPSIVKVFHGAFMDIIWLQRDLGLYVVGLFDTYHASKAIGLPRHSLAYLLENFANFKTSKKYQLADWRIRPLSKPMTAYARADTHFLLNIYDQLRNKLIESNKLAGVLYESRNVAKRRFEYSKYRPLTPSSEVYSPIEKESPWKILMYQYNIPPEREVLVRELYQWRDLIARRDDESPRFVMPNQLLAALVAYTPTDVIGVVSLTNGVTEHVRQNAKLLANLIRDALRNIKNTNEEATPIPSSETKADGILLETISVPQIRDVMERFSVLCNSNISKSRAKPVTNSSILLGKILPREEHDIAYSKDGLPNKVKTEDIRIRAQNFKSALANLEDIIFEIEKPLVVPVKLEEIKTVDPASAPNHSPEIDNLDDLVVLKKKNIQKKQPAKEKGVTEKDAVDYSKIPNILSNKPGQNNRQQKKRRFDPSSSDSNGPRAAKKRRPAAKGKNLSFKR B19A0B2ED74C6DA7 prp16_yeast Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 PRP16 YKR406 YKR086W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGHSGREERIKDIFKELTSKELTPGLLLTLQKLAQKPNTNLEQFIASCKALTKLSSNNPIIFNELLELLKNKSEEDSTGPKKIAPSINKRKKFKIQLDLDDNEDELDSPVQKKPAPTRTLFKRIDKLKAKQLRQYSPTVKDPSPNSEQQTQNGHAETKDYEPTRSEVVEEDREWYDNDDDYGNLVPEPLSELPEEAKLLPVIRNIDNDDALRNTVQLYPIPLKQRMEWIPPFLSKFALENKVPTSIIIGSISETSSQVSALSMVNPFRNPDSEFSANAKRGSKLVALRRINMEHIQQSRDNTTVLNTAMGEVLGLENNNKAKDKSNQKICDDTALFTPSKDDIKHTKEQLPVFRCRSQLLSLIRENQVVVIIGETGSGKTTQLAQYLYEEGYANDRGKSIVVTQPRRVAAISVAKRVAMEMQVPLGKEVGYSIRFEDVTDSECTKLKFVTDGILLRETLLDDTLDKYSCVIIDEAHERSLNTDILLGFFKILLARRRDLKLIITSATMNAKKFSAFFGNAPQFTIPGRTFPVQTIYTSNPVQDYVEAAVSQAVKIHLANDCSSGDILIFMTGQEDIETTFDTLQEKFLQVYSKKFGTANFEEINDIEILPIYSALPADLQFKIFQDLHGTKRKIIIATNIAETSLTIKGIRYVIDCGYSKLKVYNPKIGLDSLVITPISKANADQRSGRAGRTAPGTAYRLYTEDTFKEDMYLQTIPEIQRTNLSNTLLLLKSLDVTDELSKFPFIDKPPLQTFLSSLYELWFIGAIDTSGQLTPLGLQMAKFPLQPSLSKILLIAVRNGCSDEMLTIVSMLSVPQVFYRPKERQKEADIARNKFFIAKSDHLTLLNVFEQWRANNFSSHWCNKHFVQYKSLVRARDIRDQLLTILKSQKIPVISSGKDWDIIKKCICSGFAHQAAKITGLRNYVHLKTGVSVQLHPTSALHGLGDLPPYVVYHELLMTSKEYICCVTSVDPFWLMEYGGLLYDIKRIKNDQEATTTGLFGEHYEHTLDKVEDDIDINIRRCKDMRDSVIQELKMTDNSNKEDKKQKTKKQNILNGKENSMKPFKRRKPFF hul4_yeast Probable E3 ubiquitin-protein ligase HUL4 HECT ubiquitin ligase 4 HUL4 HECT-type E3 ubiquitin transferase HUL4 J1608 YJR036C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSLFDKLNAKKDGRDGSVSKELLSHSVAHTKNRLPKSGRRTSERSLAASVKDGSCSNSKSNKRNSSASVSGEEDKSCLISLNCLCCGVPLRFPASITKFRCSACQVTVIVKEPEINSNLESSTHISCTLEGLQMVVRRCHDDLQRLKKTGILDKERKGLIFQPVITYLLDRFHDVSILNRSFLVHDGGKNIKMLNYEVLQRFYSILSNLPTRKPYYSMLCCCNDLLKRITINKGENLQILQYRWLLIILNIPTIRTCLIRDRKSKNVFETQQIRAVSYELAKRCIGYLSNLSTKTSQQLIQSLRRTPTDNFSYQVEILNLYINFQFSRLLSNELSNRTAKNNVKPEDEMRSRLRRHHTTGHEFLSTRPISAQSNDKQGSGFTHPVNNKMKFKFFQYEEDWHIHSAAKLTFIYYVANTRRNGRGALSIQSFYNITLDFIDYKQDFDHWRGVAQKTKMNQLIEEWGNSTTKKCFSFCKYPFILSLGIKISIMEYEIRRIMEHEAEQAFLISLDKGKSVDVYFKIKVRRDVISHDSLRCIKEHQGDLLKSLRIEFVNEPGIDAGGLRKEWFFLLTKSLFNPMNGLFIYIKESSRSWFAIDPPNFDKSKGKNSQLELYYLFGVVMGLAIFNSTILDLQFPKALYKKLCSEPLSFEDYSELFPETSRNLIKMLNYTEDNFEDVFSLTFETTYRNNNWILNDSKSSKEYVTVELCENGRNVPITQSNKHEFVMKWVEFYLEKSIEPQYNKFVSGFKRVFAECNSIKLFNSEELERLVCGDEEQTKFDFKSLRSVTKYVGGFSDDSRAVCWFWEIIESWDYPLQKKLLQFVTASDRIPATGISTIPFKISLLGSHDSDDLPLAHTCFNEICLWNYSSKKKLELKLLWAINESEGYGFR air1-trf4-1 790329 n/a 790332 unspecified role unspecified role bait bait n-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 790333 unspecified role unspecified role prey prey n/a 790334 unspecified role unspecified role prey prey physical association physical association false false false Fig 3D air2-trf4-1 790329 n/a 790334 unspecified role unspecified role prey prey n/a 790335 unspecified role unspecified role bait bait n-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 790333 unspecified role unspecified role prey prey physical association physical association false false false Fig 3D mtr4-trf4-1 790329 n/a 790333 unspecified role unspecified role bait bait n-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 790334 unspecified role unspecified role prey prey physical association physical association false false false Fig 3D, S2B trf4-mtr4-1 790329 n/a 790333 unspecified role unspecified role prey prey n/a 790334 unspecified role unspecified role bait bait n-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 3D, S4 trf5-mtr4-2 790329 n/a 790336 unspecified role unspecified role bait bait n-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 790333 unspecified role unspecified role prey prey physical association physical association false false false Fig 3D rrp6-mtr4-1 790329 n/a 790333 unspecified role unspecified role prey prey n/a 790337 unspecified role unspecified role bait bait n-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig S2B pap2-prp16-1 790330 n/a 790338 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 790334 unspecified role unspecified role bait bait physical association physical association false false false Table S1 pap2-air1-2 790330 n/a 790334 unspecified role unspecified role bait bait n/a 790332 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S1 pap2-air2-1 790330 n/a 790335 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 790334 unspecified role unspecified role bait bait physical association physical association false false false Table S1 pap2-air1-1 790331 n/a 790339 unspecified role unspecified role prey prey n/a 790333 unspecified role unspecified role prey prey n/a 790335 unspecified role unspecified role prey prey n/a 790332 unspecified role unspecified role prey prey n/a 790334 unspecified role unspecified role bait bait n-terminus protein a tag protein a tag protein A protein A n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 3A