UniProt UniProt http://www.uniprot.org fischer-2004-9 Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Fischer T., Rodriguez-Navarro S., Pereira G., Racz A., Schiebel E., Hurt E. Following pull-down on IgG sepharose and TEV elution, the eluted proteins were subsequently further purified by GST pull down. cg5@ix.urz.uni-hiedelberg.de Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation fischer-2004-1 Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Fischer T., Rodriguez-Navarro S., Pereira G., Racz A., Schiebel E., Hurt E. Following pull-down on IgG sepharose and TEV elution, the eluted proteins were subsequently further purified by GST pull down. cg5@ix.urz.uni-hiedelberg.de Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation fischer-2004-2 Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap western blot western blot Immuno blot Immuno blot Fischer T., Rodriguez-Navarro S., Pereira G., Racz A., Schiebel E., Hurt E. Following pull-down on IgG sepharose and TEV elution, the eluted proteins were subsequently further purified by GST pull down. cg5@ix.urz.uni-hiedelberg.de Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation fischer-2004-3 Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Fischer T., Rodriguez-Navarro S., Pereira G., Racz A., Schiebel E., Hurt E. Following pull-down on IgG sepharose and TEV elution, the eluted proteins were subsequently further purified by GST pull down. cg5@ix.urz.uni-hiedelberg.de Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation fischer-2004-4 Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight silver stain molecular weight estimation by silver staining weight silver stain Fischer T., Rodriguez-Navarro S., Pereira G., Racz A., Schiebel E., Hurt E. Following pull-down on IgG sepharose and TEV elution, the eluted proteins were subsequently further purified by GST pull down. cg5@ix.urz.uni-hiedelberg.de Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation fischer-2004-5 Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down ms participant Identification by mass spectrometry ms participant Fischer T., Rodriguez-Navarro S., Pereira G., Racz A., Schiebel E., Hurt E. Following pull-down on IgG sepharose and TEV elution, the eluted proteins were subsequently further purified by GST pull down. cg5@ix.urz.uni-hiedelberg.de Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation fischer-2004-6 Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Fischer T., Rodriguez-Navarro S., Pereira G., Racz A., Schiebel E., Hurt E. Following pull-down on IgG sepharose and TEV elution, the eluted proteins were subsequently further purified by GST pull down. cg5@ix.urz.uni-hiedelberg.de Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation fischer-2004-7 Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down ms participant Identification by mass spectrometry ms participant Fischer T., Rodriguez-Navarro S., Pereira G., Racz A., Schiebel E., Hurt E. Following pull-down on IgG sepharose and TEV elution, the eluted proteins were subsequently further purified by GST pull down. cg5@ix.urz.uni-hiedelberg.de Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation fischer-2004-8 Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Fischer T., Rodriguez-Navarro S., Pereira G., Racz A., Schiebel E., Hurt E. Following pull-down on IgG sepharose and TEV elution, the eluted proteins were subsequently further purified by GST pull down. cg5@ix.urz.uni-hiedelberg.de Nat. Cell Biol. (1465-7392) 2004 Only protein-protein interactions imex curation cdc31_yeast Cell division control protein 31 YOR257W CDC31 DSK1 Nucleoporin CDC31 Nuclear pore protein CDC31 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKNRSSLQSGPLNSELLEEQKQEIYEAFSLFDMNNDGFLDYHELKVAMKALGFELPKREILDLIDEYDSEGRHLMKYDDFYIVMGEKILKRDPLDEIKRAFQLFDDDHTGKISIKNLRRVAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAICTDS 25A546E76E1EF520 thp1_yeast Nuclear mRNA export protein THP1 Bud site selection protein 29 THP1 BUD29 O1140 YOL072W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTRFKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFIINLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVNKLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAFVQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWSVLYKHVRYGNIQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQNKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQLQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW 7026F1F6FCC73FFA sus1_yeast Transcription and mRNA export factor SUS1 SUS1 YBR111W-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MTMDTAQLKSQIQQYLVESGNYELISNELKARLLQEGWVDKVKDLTKSEMNINESTNFTQILSTVEPKALEMVSDSTRETVLKQIREFLEEIVDTQ DB0DA0C9DC13FB21 sac3_yeast Nuclear mRNA export protein SAC3 SAC3 LEP1 YDR159W YD8358.13 Leucine permease transcriptional regulator protein protein yeast Saccharomyces cerevisiae Baker's yeast MNTSFGSVVPSTNFNFFKGHGNNDNTSANSTVNNSNFFLNSNETKPSKNVFMVHSTSQKKSQQPLQNLSHSPSYTENKPDKKKKYMINDAKTIQLVGPLISSPDNLGFQKRSHKARELPRFLINQEPQLEKRAFVQDPWDKANQEKMISLEESIDDLNELYETLKKMRNTERSIMEEKGLVDKADSAKDLYDAIVFQGTCLDMCPTFERSRRNVEYTVYSYEKNQPNDKKASRTKALKVFARPAAAAAPPLPSDVRPPHILVKTLDYIVDNLLTTLPESEGFLWDRMRSIRQDFTYQNYSGPEAVDCNERIVRIHLLILHIMVKSNVEFSLQQELEQLHKSLITLSEIYDDVRSSGGTCPNEAEFRAYALLSKIRDPQYDENIQRLPKHIFQDKLVQMALCFRRVISNSAYTERGFVKTENCLNFYARFFQLMQSPSLPLLMGFFLQMHLTDIRFYALRALSHTLNKKHKPIPFIYLENMLLFNNRQEIIEFCNYYSIEIINGDAADLKTLQHYSHKLSETQPLKKTYLTCLERRLQKTTYKGLINGGEDNLASSVYVKDPKKDRIPSIADQSFLMENFQNNYNEKLNQNSSVKPQINTSPKRVATRPNHFPFSQESKQLPQISQSHTLSTNPLLTPQVHGDLSEQKQQQIKTVTDGGSPFVFDQSAQNSTVEASKAHMISTTSNGAYDEKLSSEQEEMRKKEEQRIEEEKTQLKKKQENADKQVITEQIANDLVKEVVNSSVISIVKREFSEANYRKDFIDTMTRELYDAFLHERLYLIYMDSRAELKRNSTLKKKFFEKWQASYSQAKKNRILEEKKREEIKLVSHQLGVPGFKKSTCLFRTPYKGNVNSSFMLSSSDKNLIFSPVNDEFNKFATHLTKISKLWRPLEMQSIYYDNLTKKFPSNSLTPANLFIYAKDWTSLSNRWILSKFNLQTAQDSKKFSNNIISSRIICIDDEYEPSDFSDLQLLIFNTGVTNPDIFDLEMKLKDDGEELIKLITGISLNTNICFSLLIIYWESAENTLSESTIKHLLKLNRISKNYSSVIERIDLMNLTEESPHKCLEDKLSEISHSYVYKLTERGKYDKTLRQKRSLAGIHSRSTQLQTTKDIDQKMKKMLEKEKNKYQQQIGERNTYAHLESHIDASPRSKKRKLPILLSTSHSSQFKTPLASRLNTSGSSTSPPLPSHLAMKFRKNSRVTSLHTVLPVSTPSHSNNIPAASFSGNNTTDIQSQQLIENQKSTSVYLNNVSERILGNQEICQTPINPVTPVLDGADQGKEDIPDSILELKILIDSVKKKVNND 0679DB1673DDACEB sfi1_yeast Protein SFI1 SFI1 Suppressor of fermentation induced loss of stress resistance protein 1 L1373 YLL003W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKFGTTNKSTENLLRDKFVPETSPTNIPTDVLIKQGQITDSTESLIHGGAERYIVNALKPIELNKTEGFFEDPPFHLPSPPVDSTNLEYEDVTDLPKNGLRYDLNDISVEVIEDLYRQIEAFLVHFKLSRSFLQIFKNYVNILIQEGINPLRDEYFTILEDELKGFFTFNSVIEEILEIFLIHPRNKFIALSLAEYTYAKNKIRRHFNHWKTVCELNEEANRFANQAKLRVQEAVFYIWSDKTLKYSQMANDEAESFRNTWLLFRSFQQWITLTQTLKEQSRLADQAFLNKMFRKILKAQEHWKHLETVNTDNIKKIFLRTTFHIWKLRHKEINYHGLERRIFERIKQKVINYEYNKSIAEKVRSFSLQRKYLNKWEKKNIENEDKLGALYELENKFIKQKFFRKLNRSFQHSQQEAIAKSKLNQTLLRCVFEKMWLKRFEDHLHLYSIVSLKEANLVKRIFHSWKKLLYIDLKASDYSRTNLLKSSLRSWKLEVKLKIFEQKCKKSIQASAYRTWRKRIQYGKISSEHVKTAFCAKYLGVWKRRMLQMNSMNDEASKFYEEGLVNECLAIWKERLIKTKELEDRYNFLCKTHAILTVKRTLMHIDNVHLLYTKLAPSMDRVKLSKAFLKWRKATRFKVRHKLNDILHVYEKSKERELQSQLFNAWRNRFCFYTEECNIQAISKRNYQLEKMVLKKFRERLLEIVKSEELADEVREEFVLVKTFYIWKTHLDEIFYMSTLLEQSEANKQFIITSKFLKMWSLRFLKIKRNDETVEVFRHRWDRATVRGLLLLWKNRSDSSPKRRKDFNLKHELKTPIRSDSQNASTIPGSERIKQHRMEAMKSHYSRARRAIPSPVKSSSVLDSTAKKQINLESTTGLNGSPTRGKPLRYSPRRTTRNMPSKVDHIDFGRIPAVPFSLSANSPKIDQDMDYIREHDKSPLSRKRQ spt7_yeast Transcriptional activator SPT7 YBR0739 YBR081C SPT7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTERIPIKNYQRTNAKALLKLTEKLFNKNFFDLYLTSQQLVVLEYLLSISSEEDKLKAWDYFLKGNIALNVEKSFPLTQEEEHHGAVSPAVDTRSDDVSSQTIKDNNNTNTNTSISNENHVENEIEDKGDNAIANEDNFVNNDESDNVEEDLFKLDLEDLKQQISGTRFIGNLSLKIRYVLWQCAIDYIYCDRNEFGDENDTEYTLLDVEEKEEEEIGKNEKPQNKEGISKFAEDEDYDDEDENYDEDSTDVKNVDDPPKNLDSISSSNIEIDDERRLVLNISISKETLSKLKTNNVEEIMGNWNKIYHSFEYDKETMIKRLKLEESDKMIEKGKKKRSRSDLEAATDEQDRENTNDEPDTNQKLPTPEGSTFSDTGNKRPKQSNLDLTVNLGIENLSLKHLLSSIQQKKSQLGISDYELKHLIMDVRKNRSKWTSDERIGQEELYEACEKVVLELRNYTEHSTPFLNKVSKREAPNYHQIIKKSMDLNTVLKKLKSFQYDSKQEFVDDIMLIWKNCLTYNSDPSHFLRGHAIAMQKKSLQLIRMIPNITIRNRADLEKEIEDMEKDKDYELDEEEEVAGSGRKGLNMGAHMLAKENGKVSEKDSSKTVKDEAPTNDDKLTSVIPEGEKEKDKTASSTVTVHENVNKNEIKENGKNEEQDMVEESSKTEDSSKDADAAKKDTEDGLQDKTAENKEAGENNEEEEDDDDEDEDEDMVDSQSYLLEKDDDRDDLEISVWKTVTAKVRAEICLKRTEYFKNGKLNSDSEAFLKNPQRMKRFDQLFLEYKEQKALESYRQKIEQNSIMKNGFGTVLKQEDDDQLQFHNDHSLNGNEAFEKQPNDIELDDTRFLQEYDISNAIPDIVYEGVNTKTLDKMEDASVDRMLQNGINKQSRFLANKDLGLTPKMNQNITLIQQIRHICHKISLIRMLQSPLSAQNSRSNPNAFLNNHIYNYTIIDDSLDIDPVSQLPTHDYKNNRELIWKFMHKNISKVAMANGFETAHPSAINMLTEIAGDYLSNLIKTLKLHHETNSLNRGTNVEMLQTTLLENGINRPDDLFSYVESEFGKKTKKLQDIKQKLESFLRALLRPTLQELSERNFEDESQSFFTGDFASELTGEDFFGFRELGLEKEFGVLSSSVPLQLLTTQFQTVDGETKVQAKKIQPEESDSIVYKKITKGMLDAGSFWNTLLPLLQKDYERSKAYIAKQSKSSANDKTSMTSTEDNSFALLEEDQFVSKKTATKARLPPTGKISTTYKKKPIASAFILPEEDLENDVKADPTTTVNAKVGAENDGDSSLFLRTPQPLDPLDMDDAFDDTNMGSNSSFSLSLPRLNQ 083B63624669244F tra1_yeast Transcription-associated protein 1 TRA1 p400 kDa component of SAGA YHR099W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLTEQIEQFASRFRDDDATLQSRYSTLSELYDIMELLNSPEDYHFFLQAVIPLLLNQLKEVPISYDAHSPEQKLRNSMLDIFNRCLMNQTFQPYAMEVLEFLLSVLPKENEENGILCMKVLTTLFKSFKSILQDKLDSFIRIIIQIYKNTPNLINQTFYEAGKAEQGDLDSPKEPQADELLDEFSKNDEEKDFPSKQSSTEPRFENSTSSNGLRSSMFSFKILSECPITMVTLYSSYKQLTSTSLPEFTPLIMNLLNIQIKQQQEAREQAESRGEHFTSISTEIINRPAYCDFILAQIKATSFLAYVFIRGYAPEFLQDYVNFVPDLIIRLLQDCPSELSSARKELLHATRHILSTNYKKLFLPKLDYLFDERILIGNGFTMHETLRPLAYSTVADFIHNIRSELQLSEIEKTIKIYTGYLLDESLALTVQIMSAKLLLNLVERILKLGKENPQEAPRAKKLLMIIIDSYMNRFKTLNRQYDTIMKYYGRYETHKKEKAEKLKNSIQDNDKESEEFMRKVLEPSDDDHLMPQPKKEDINDSPDVEMTESDKVVKNDVEMFDIKNYAPILLLPTPTNDPIKDAFYLYRTLMSFLKTIIHDLKVFNPPPNEYTVANPKLWASVSRVFSYEEVIVFKDLFHECIIGLKFFKDHNEKLSPETTKKHFDISMPSLPVSATKDARELMDYLAFMFMQMDNATFNEIIEQELPFVYERMLEDSGLLHVAQSFLTSEITSPNFAGILLRFLKGKLKDLGNVDFNTSNVLIRLFKLSFMSVNLFPNINEVVLLPHLNDLILNSLKYSTTAEEPLVYFYLIRTLFRSIGGGRFENLYRSIKPILQVLLQSLNQMILTARLPHERELYVELCITVPVRLSVLAPYLPFLMKPLVFALQQYPDLVSQGLRTLELCIDNLTAEYFDPIIEPVIDDVSKALFNLLQPQPFNHAISHNVVRILGKLGGRNRQFLKPPTDLTEKTELDIDAIADFKINGMPEDVPLSVTPGIQSALNILQSYKSDIHYRKSAYKYLTCVLLLMTKSSAEFPTNYTELLKTAVNSIKLERIGIEKNFDLEPTVNKRDYSNQENLFLRLLESVFYATSIKELKDDAMDLLNNLLDHFCLLQVNTTLLNKRNYNGTFNIDLKNPNFMLDSSLILDAIPFALSYYIPEVREVGVLAYKRIYEKSCLIYGEELALSHSFIPELAKQFIHLCYDETYYNKRGGVLGIKVLIDNVKSSSVFLKKYQYNLANGLLFVLKDTQSEAPSAITDSAEKLLIDLLSITFADVKEEDLGNKVLENTLTDIVCELSNANPKVRNACQKSLHTISNLTGIPIVKLMDHSKQFLLSPIFAKPLRALPFTMQIGNVDAITFCLSLPNTFLTFNEELFRLLQESIVLADAEDESLSTNIQKTTEYSTSEQLVQLRIACIKLLAIALKNEEFATAQQGNIRIRILAVFFKTMLKTSPEIINTTYEALKGSLAENSKLPKELLQNGLKPLLMNLSDHQKLTVPGLDALSKLLELLIAYFKVEIGRKLLDHLTAWCRVEVLDTLFGQDLAEQMPTKIIVSIINIFHLLPPQADMFLNDLLLKVMLLERKLRLQLDSPFRTPLARYLNRFHNPVTEYFKKNMTLRQLVLFMCNIVQRPEAKELAEDFEKELDNFYDFYISNIPKNQVRVVSFFTNMVDLFNTMVITNGDEWLKKKGNMILKLKDMLNLTLKTIKENSFYIDHLQLNQSIAKFQALYLRFTELSERDQNPLLLDFIDFSFSNGIKASYSLKKFIFHNIIASSNKEKQNNFINDATLFVLSDKCLDARIFVLKNVINSTLIYEVATSGSLKSYLVEDKKPKWLELLHNKIWKNSNAILAYDVLDHHDLFRFELLQLSAIFIKADPEIIAEIKKDIIKFCWNFIKLEDTLIKQSAYLVTSYFISKFDFPIKVVTQVFVALLRSSHVEARYLVKQSLDVLTPVLHERMNAAGTPDTWINWVKRVMVENSSSQNNILYQFLISHPDLFFNSRDLFISNIIHHMNKITFMSNSNSDSHTLAIDLASLILYWENKTLEITNVNNTKTDSDGDVVMSDSKSDINPVEADTTAIIVDANNNSPISLHLREACTAFLIRYVCASNHRAIETELGLRAINILSELISDKHWTNVNVKLVYFEKFLIFQDLDSENILYYCMNALDVLYVFFKNKTKEWIMENLPTIQNLLEKCIKSDHHDVQEALQKVLQVIMKAIKAQGVSVIIEEESPGKTFIQMLTSVITQDLQETSSVTAGVTLAWVLFMNFPDNIVPLLTPLMKTFSKLCKDHLSISQPKDAMALEEARITTKLLEKVLYILSLKVSLLGDSRRPFLSTVALLIDHSMDQNFLRKIVNMSRSWIFNTEIFPTVKEKAAILTKMLAFEIRGEPSLSKLFYEIVLKLFDQEHFNNTEITVRMEQPFLVGTRVEDIGIRKRFMTILDNSLERDIKERLYYVIRDQNWEFIADYPWLNQALQLLYGSFNREKELSLKNIYCLSPPSILQEYLPENAEMVTEVNDLELSNFVKGHIASMQGLCRIISSDFIDSLIEIFYQDPKAIHRAWVTLFPQVYKSIPKNEKYGFVRSIITLLSKPYHTRQISSRTNVINMLLDSISKIESLELPPHLVKYLAISYNAWYQSINILESIQSNTSIDNTKIIEANEDALLELYVNLQEEDMFYGLWRRRAKYTETNIGLSYEQIGLWDKAQQLYEVAQVKARSGALPYSQSEYALWEDNWIQCAEKLQHWDVLTELAKHEGFTDLLLECGWRVADWNSDRDALEQSVKSVMDVPTPRRQMFKTFLALQNFAESRKGDQEVRKLCDEGIQLSLIKWVSLPIRYTPAHKWLLHGFQQYMEFLEATQIYANLHTTTVQNLDSKAQEIKRILQAWRDRLPNTWDDVNMWNDLVTWRQHAFQVINNAYLPLIPALQQSNSNSNINTHAYRGYHEIAWVINRFAHVARKHNMPDVCISQLARIYTLPNIEIQEAFLKLREQAKCHYQNMNELTTGLDVISNTNLVYFGTVQKAEFFTLKGMFLSKLRAYEEANQAFATAVQIDLNLAKAWAQWGFFNDRRLSEEPNNISFASNAISCYLQAAGLYKNSKIRELLCRILWLISIDDASGMLTNAFDSFRGEIPVWYWITFIPQLLTSLSHKEANMVRHILIRIAKSYPQALHFQLRTTKEDFAVIQRQTMAVMGDKPDTNDRNGRRQPWEYLQELNNILKTAYPLLALSLESLVAQINDRFKSTTDEDLFRLINVLLIDGTLNYNRLPFPRKNPKLPENTEKNLVKFSTTLLAPYIRPKFNADFIDNKPDYETYIKRLRYWRRRLENKLDRASKKENLEVLCPHLSNFHHQKFEDIEIPGQYLLNKDNNVHFIKIARFLPTVDFVRGTHSSYRRLMIRGHDGSVHSFAVQYPAVRHSRREERMFQLYRLFNKSLSKNVETRRRSIQFNLPIAIPLSPQVRIMNDSVSFTTLHEIHNEFCKKKGFDPDDIQDFMADKLNAAHDDALPAPDMTILKVEIFNSIQTMFVPSNVLKDHFTSLFTQFEDFWLFRKQFASQYSSFVFMSYMMMINNRTPHKIHVDKTSGNVFTLEMLPSRFPYERVKPLLKNHDLSLPPDSPIFHNNEPVPFRLTPNIQSLIGDSALEGIFAVNLFTISRALIEPDNELNTYLALFIRDEIISWFSNLHRPIIENPQLREMVQTNVDLIIRKVAQLGHLNSTPTVTTQFILDCIGSAVSPRNLARTDVNFMPWF AE3588676F5D5777 cdc31-cdc31-1 794260 n/a 794269 unspecified role unspecified role neutral component neutral component n/a 794269 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig 7B cdc31-thp1-1 794260 n/a 794270 unspecified role unspecified role neutral component neutral component n/a 794269 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig 7B cdc31-sus1-1 794260 n/a 794269 unspecified role unspecified role neutral component neutral component n/a 794271 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig 7B sac3-cdc31-1 794261 n/a 794272 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 794269 unspecified role unspecified role prey prey n/a 794271 unspecified role unspecified role prey prey physical association physical association false false false Fig 2A cdc31-sac3-1 794261 n/a 794273 unspecified role unspecified role prey prey n/a 794270 unspecified role unspecified role prey prey n/a 794269 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794269 unspecified role unspecified role prey prey n/a 794272 unspecified role unspecified role prey prey physical association physical association false false false Fig 4C sac3-cdc31-2 794262 n/a 794272 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794269 unspecified role unspecified role prey prey physical association physical association false false false Fig 2B, 2C sac3-sus1-1 794262 n/a 794272 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794271 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 2B sus1-cdc31-1 794263 n/a 794274 unspecified role unspecified role prey prey n/a 794269 unspecified role unspecified role prey prey n/a 794275 unspecified role unspecified role prey prey n/a 794272 unspecified role unspecified role prey prey n/a 794271 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794270 unspecified role unspecified role prey prey c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 4A sus1-cdc31-2 794264 n/a 794270 unspecified role unspecified role neutral component neutral component c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794271 unspecified role unspecified role neutral component neutral component c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794269 unspecified role unspecified role neutral component neutral component n/a 794272 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig 4B sac3-cdc31-3 794265 n/a 794269 unspecified role unspecified role prey prey n/a 794270 unspecified role unspecified role prey prey c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794271 unspecified role unspecified role prey prey c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794272 unspecified role unspecified role bait bait n-terminus prota tev tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 7A The CID domain of SAC3 (734-850) is required for interaction between SAC3 and CDC31 and SUS1. sac3-cdc31-4 794266 n/a 794269 unspecified role unspecified role prey prey n/a 794272 unspecified role unspecified role bait bait n-terminus prota tev tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 794271 unspecified role unspecified role prey prey c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794270 unspecified role unspecified role prey prey c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 7A The CID domain of SAC3 (734-850) is required for interaction between SAC3 and CDC31 and SUS1. sac3-cdc31-5 794267 n/a 794271 unspecified role unspecified role prey prey c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794269 unspecified role unspecified role prey prey n/a 794272 unspecified role unspecified role bait bait n-terminus prota tev tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 794270 unspecified role unspecified role prey prey c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794275 unspecified role unspecified role prey prey n/a 794274 unspecified role unspecified role prey prey physical association physical association false false false Fig 7A The CID domain of SAC3 (734-850) is required for interaction between SAC3 and CDC31 and SUS1. sac3-cdc31-6 794268 n/a 794270 unspecified role unspecified role prey prey c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 794272 unspecified role unspecified role bait bait n-terminus prota tev tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 794269 unspecified role unspecified role prey prey n/a 794271 unspecified role unspecified role prey prey c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 7A The CID domain of SAC3 (734-850) is required for interaction between SAC3 and CDC31 and SUS1.