IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ thierse-2004-1 Metal-protein complex-mediated transport and delivery of Ni2+ to TCR/MHC contact sites in nickel-specific human T cell activation. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Thierse HJ., Moulon C., Allespach Y., Zimmermann B., Doetze A., Kuppig S., Wild D., Herberg F., Weltzien HU. SPR signal measured in relative units was taken as a measure of author confidence in the interaction thierse@immunbio.mpg.de 2009-03-30: Hermann-Josef Thierse, Dept. of Dermatoloy, Univ. Heidelberg, Germany. J. Immunol. (0022-1767) 2004 Only protein-protein interactions imex curation albu_human Albumin GIG42 ALB PRO0903 PRO1708 PRO2044 PRO2619 PRO2675 UNQ696/PRO1341 GIG20 protein protein human Homo sapiens Human MKWVTFISLLFLFSSAYSRGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL nickel(2+) Nickel(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) albu_bovin Albumin ALB BSA protein protein bovin Bos taurus (Bovine) Bovine MKWVTFISLLLLFSSAYSRGVFRRDTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGACLLPKIETMREKVLASSARQRLRCASIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVVSTQTALA albu_chick Albumin ALB Alpha-livetin protein protein chick Gallus gallus (Chicken) Chicken MKWVTLISFIFLFSSATSRNLQRFARDAEHKSEIAHRYNDLKEETFKAVAMITFAQYLQRCSYEGLSKLVKDVVDLAQKCVANEDAPECSKPLPSIILDEICQVEKLRDSYGAMADCCSKADPERNECFLSFKVSQPDFVQPYQRPASDVICQEYQDNRVSFLGHFIYSVARRHPFLYAPAILSFAVDFEHALQSCCKESDVGACLDTKEIVMREKAKGVSVKQQYFCGILKQFGDRVFQARQLIYLSQKYPKAPFSEVSKFVHDSIGVHKECCEGDMVECMDDMARMMSNLCSQQDVFSGKIKDCCEKPIVERSQCIMEAEFDEKPADLPSLVEKYIEDKEVCKSFEAGHDAFMAEFVYEYSRRHPEFSIQLIMRIAKGYESLLEKCCKTDNPAECYANAQEQLNQHIKETQDVVKTNCDLLHDHGEADFLKSILIRYTKKMPQVPTDLLLETGKKMTTIGTKCCQLGEDRRMACSEGYLSIVIHDTCRKQETTPINDNVSQCCSQLYANRRPCFTAMGVDTKYVPPPFNPDMFSFDEKLCSAPAEEREVGQMKLLINLIKRKPQMTEEQIKTIADGFTAMVDKCCKQSDINTCFGEEGANLIVQSRATLGIGA albu_pig Albumin ALB protein protein pig Sus scrofa (Pig) Pig MKWVTFISLLFLFSSAYSRGVFRRDTYKSEIAHRFKDLGEQYFKGLVLIAFSQHLQQCPYEEHVKLVREVTEFAKTCVADESAENCDKSIHTLFGDKLCAIPSLREHYGDLADCCEKEEPERNECFLQHKNDNPDIPKLKPDPVALCADFQEDEQKFWGKYLYEIARRHPYFYAPELLYYAIIYKDVFSECCQAADKAACLLPKIEHLREKVLTSAAKQRLKCASIQKFGERAFKAWSLARLSQRFPKADFTEISKIVTDLAKVHKECCHGDLLECADDRADLAKYICENQDTISTKLKECCDKPLLEKSHCIAEAKRDELPADLNPLEHDFVEDKEVCKNYKEAKHVFLGTFLYEYSRRHPDYSVSLLLRIAKIYEATLEDCCAKEDPPACYATVFDKFQPLVDEPKNLIKQNCELFEKLGEYGFQNALIVRYTKKVPQVSTPTLVEVARKLGLVGSRCCKRPEEERLSCAEDYLSLVLNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYKPKEFVEGTFTFHADLCTLPEDEKQIKKQTALVELLKHKPHATEEQLRTVLGNFAAFVQKCCAAPDHEACFAVEGPKFVIEIRGILA albu_mouse Albumin Alb Alb1 Alb-1 protein protein mouse Mus musculus Mouse MKWVTFLLLLFVSGSAFSRGVFRREAHKSEIAHRYNDLGEQHFKGLVLIAFSQYLQKCSYDEHAKLVQEVTDFAKTCVADESAANCDKSLHTLFGDKLCAIPNLRENYGELADCCTKQEPERNECFLQHKDDNPSLPPFERPEAEAMCTSFKENPTTFMGHYLHEVARRHPYFYAPELLYYAEQYNEILTQCCAEADKESCLTPKLDGVKEKALVSSVRQRMKCSSMQKFGERAFKAWAVARLSQTFPNADFAEITKLATDLTKVNKECCHGDLLECADDRAELAKYMCENQATISSKLQTCCDKPLLKKAHCLSEVEHDTMPADLPAIAADFVEDQEVCKNYAEAKDVFLGTFLYEYSRRHPDYSVSLLLRLAKKYEATLEKCCAEANPPACYGTVLAEFQPLVEEPKNLVKTNCDLYEKLGEYGFQNAILVRYTQKAPQVSTPTLVEAARNLGRVGTKCCTLPEDQRLPCVEDYLSAILNRVCLLHEKTPVSEHVTKCCSGSLVERRPCFSALTVDETYVPKEFKAETFTFHSDICTLPEKEKQIKKQTALAELVKHKPKATAEQLKTVMDDFAQFLDTCCKAADKDTCFSTEGPNLVTRCKDALA his-his Histidine dipepetide protein protein in vitro In vitro HH An amino acid sequence which is not represented in UniProt ni2plus-albu-1 Binding of Ni2+ to human serum albumin 803617 n/a 803618 unspecified role unspecified role prey prey human-serum Homo sapiens serum Serum n/a 803619 unspecified role unspecified role bait bait direct interaction direct interaction false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 1425 RU Figs. 3A, 3B 1425 RU ni2plus-albu-2 Binding of Ni2+ to bovine serum albumin 803617 n/a 803620 unspecified role unspecified role prey prey bovin-serum Bovine serum serum Serum n/a 803619 unspecified role unspecified role bait bait direct interaction direct interaction false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 326 RU Fig. 3A 326 RU ni2plus-albu-3 Binding of Ni2+ to chick serum albumin 803617 n/a 803619 unspecified role unspecified role bait bait n/a 803621 unspecified role unspecified role prey prey chick-serum Chick serum serum Serum direct interaction direct interaction false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 167 RU Fig. 3A 167 RU ni2plus-albu-4 Binding of Ni2+ to pig serum albumin 803617 n/a 803619 unspecified role unspecified role bait bait n/a 803622 unspecified role unspecified role prey prey pig-serum Pig serum serum Serum direct interaction direct interaction false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 220 RU Fig. 3A 220 RU ni2plus-albu-5 Binding of Ni2+ to mouse serum albumin 803617 n/a 803623 unspecified role unspecified role prey prey mouse-serum Mouse serum serum Serum n/a 803619 unspecified role unspecified role bait bait direct interaction direct interaction false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 205 RU Fig. 3A 205 RU ni2plus-albu-6 Binding of Ni2+ to human serum albumin 803617 n/a 803619 unspecified role unspecified role bait bait n/a 803618 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Figs. 3C Temp.=20C, pH=7.4 ni2plus-albu-7 Binding of Ni2+ to human serum albumin - competition by a synthetic HSA peptide 803617 n/a 803618 unspecified role unspecified role prey prey human-serum Homo sapiens serum Serum n/a 803618 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 803619 unspecified role unspecified role bait bait physical association physical association false false false Figs. 3D The synthetic HSA tetrapeptide competed with full length HSA for nickel binding. ni2plus-albu-8 Binding of Ni2+ to human serum albumin - competition by a synthetic histidine peptide 803617 n/a 803619 unspecified role unspecified role bait bait n/a 803624 unspecified role unspecified role prey prey in vitro In vitro n/a 803618 unspecified role unspecified role prey prey in vitro In vitro physical association physical association false false false Figs. 3D The synthetic HSA tetrapeptide competed with full length HSA for nickel binding.