IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ vandenbroer-2003-6 The c-Cbl-associated protein and c-Cbl are two new partners of the SH2-containing inositol polyphosphate 5-phosphatase SHIP2. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vandenbroere I., Paternotte N., Dumont JE., Erneux C., Pirson I. ilpirson@ulb.ac.be Human brain cDNA library Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation vandenbroer-2003-1 The c-Cbl-associated protein and c-Cbl are two new partners of the SH2-containing inositol polyphosphate 5-phosphatase SHIP2. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Vandenbroere I., Paternotte N., Dumont JE., Erneux C., Pirson I. ilpirson@ulb.ac.be Human brain cDNA library Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation vandenbroer-2003-2 The c-Cbl-associated protein and c-Cbl are two new partners of the SH2-containing inositol polyphosphate 5-phosphatase SHIP2. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Vandenbroere I., Paternotte N., Dumont JE., Erneux C., Pirson I. ilpirson@ulb.ac.be Human brain cDNA library Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation vandenbroer-2003-3 The c-Cbl-associated protein and c-Cbl are two new partners of the SH2-containing inositol polyphosphate 5-phosphatase SHIP2. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Vandenbroere I., Paternotte N., Dumont JE., Erneux C., Pirson I. ilpirson@ulb.ac.be Human brain cDNA library Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation vandenbroer-2003-4 The c-Cbl-associated protein and c-Cbl are two new partners of the SH2-containing inositol polyphosphate 5-phosphatase SHIP2. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Vandenbroere I., Paternotte N., Dumont JE., Erneux C., Pirson I. ilpirson@ulb.ac.be Human brain cDNA library Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation vandenbroer-2003-5 The c-Cbl-associated protein and c-Cbl are two new partners of the SH2-containing inositol polyphosphate 5-phosphatase SHIP2. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Vandenbroere I., Paternotte N., Dumont JE., Erneux C., Pirson I. ilpirson@ulb.ac.be Human brain cDNA library Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation ship2_human Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 INPPL1 SHIP2 Inositol polyphosphate phosphatase-like protein 1 Protein 51C SH2 domain-containing inositol 5'-phosphatase 2 protein protein human Homo sapiens Human MASACGAPGPGGALGSQAPSWYHRDLSRAAAEELLARAGRDGSFLVRDSESVAGAFALCVLYQKHVHTYRILPDGEDFLAVQTSQGVPVRRFQTLGELIGLYAQPNQGLVCALLLPVEGEREPDPPDDRDASDGEDEKPPLPPRSGSTSISAPTGPSSPLPAPETPTAPAAESAPNGLSTVSHDYLKGSYGLDLEAVRGGASHLPHLTRTLATSCRRLHSEVDKVLSGLEILSKVFDQQSSPMVTRLLQQQNLPQTGEQELESLVLKLSVLKDFLSGIQKKALKALQDMSSTAPPAPQPSTRKAKTIPVQAFEVKLDVTLGDLTKIGKSQKFTLSVDVEGGRLVLLRRQRDSQEDWTTFTHDRIRQLIKSQRVQNKLGVVFEKEKDRTQRKDFIFVSARKREAFCQLLQLMKNKHSKQDEPDMISVFIGTWNMGSVPPPKNVTSWFTSKGLGKTLDEVTVTIPHDIYVFGTQENSVGDREWLDLLRGGLKELTDLDYRPIAMQSLWNIKVAVLVKPEHENRISHVSTSSVKTGIANTLGNKGAVGVSFMFNGTSFGFVNCHLTSGNEKTARRNQNYLDILRLLSLGDRQLNAFDISLRFTHLFWFGDLNYRLDMDIQEILNYISRKEFEPLLRVDQLNLEREKHKVFLRFSEEEISFPPTYRYERGSRDTYAWHKQKPTGVRTNVPSWCDRILWKSYPETHIICNSYGCTDDIVTSDHSPVFGTFEVGVTSQFISKKGLSKTSDQAYIEFESIEAIVKTASRTKFFIEFYSTCLEEYKKSFENDAQSSDNINFLKVQWSSRQLPTLKPILADIEYLQDQHLLLTVKSMDGYESYGECVVALKSMIGSTAQQFLTFLSHRGEETGNIRGSMKVRVPTERLGTRERLYEWISIDKDEAGAKSKAPSVSRGSQEPRSGSRKPAFTEASCPLSRLFEEPEKPPPTGRPPAPPRAAPREEPLTPRLKPEGAPEPEGVAAPPPKNSFNNPAYYVLEGVPHQLLPPEPPSPARAPVPSATKNKVAITVPAPQLGHHRHPRVGEGSSSDEESGGTLPPPDFPPPPLPDSAIFLPPSLDPLPGPVVRGRGGAEARGPPPPKAHPRPPLPPGPSPASTFLGEVASGDDRSCSVLQMAKTLSEVDYAPAGPARSALLPGPLELQPPRGLPSDYGRPLSFPPPRIRESIQEDLAEEAPCLQGGRASGLGEAGMSAWLRAIGLERYEEGLVHNGWDDLEFLSDITEEDLEEAGVQDPAHKRLLLDTLQLSK a0a061i5t4_crigr E3 ubiquitin-protein ligase CBL H671_4g13114 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster LQLNSLGPEACRDLRDLLLHNQCQITTLRKFKFNPRLGIDNPVLSLAEDQDQSDPWNLRRPRFCLLSKEEEKSFGFHLQQQLGKADHVVCRVDPGSSAQRHGLREGDRILAVNNKNVEHEDYAMVVRCIRASGPRVLLTVLAQHAHDVVRAQQGSDVFCCPALGPEVRPRLCHIVKDEGGFGFSITHGNRGPFWLVLSSGGAAERAGVPPGARLLEVNGVCVEKFTSNQLSRKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTVLSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYLFPDGRNQNPDLTGLCEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFCRCEIKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDSLFMMKELAGAKAASGSLHKDKPLPIPPTLRDLPPPPPPDRPYSVGTETRPQRRPLPCTPGDCPSRDKLPPVPSSRPGDSWLPRPIPKVPIATPSPNDPWNGRELTNRHSLPFSLPSQMEPRVDVPRLGSTFSLDTSMNMNSSPIAGPENEHPKIKPSSSANAIYSLAARPLPVPKLPPGEQGESEEDAEYMTPTSRPVGAQKPEPKRPLEATQSSRACDCDQQIDSCTYEAMYNIQSQALSVTENSTFGEGNLATAPTSTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNFNEGSQVPERPPKPFPRRINSERKASSCQQGGATANPSATAPSPQLSSEIERLMSQGYSYQDIQKALVIAHNNIEMAKNILREFVSISSPAHVAT srbs1_human Sorbin and SH3 domain-containing protein 1 KIAA0894 Ponsin c-Cbl-associated protein SH3 domain protein 5 SH3P12 SORBS1 KIAA1296 SH3D5 protein protein human Homo sapiens Human MSSECDGGSKAVMNGLAPGSNGQDKATADPLRARSISAVKIIPVKTVKNASGLVLPTDMDLTKICTGKGAVTLRASSSYRETPSSSPASPQETRQHESKPGLEPEPSSADEWRLSSSADANGNAQPSSLAAKGYRSVHPNLPSDKSQDATSSSAAQPEVIVVPLYLVNTDRGQEGTARPPTPLGPLGCVPTIPATASAASPLTFPTLDDFIPPHLQRWPHHSQPARASGSFAPISQTPPSFSPPPPLVPPAPEDLRRVSEPDLTGAVSSTDSSPLLNEVSSSLIGTDSQAFPSVSKPSSAYPSTTIVNPTIVLLQHNREQQKRLSSLSDPVSERRVGEQDSAPTQEKPTSPGKAIEKRAKDDSRRVVKSTQDLSDVSMDEVGIPLRNTERSKDWYKTMFKQIHKLNRDTPEENPYFPTYKFPELPEIQQTSEEDNPYTPTYQFPASTPSPKSEDDDSDLYSPRYSFSEDTKSPLSVPRSKSEMSYIDGEKVVKRSATLPLPARSSSLKSSSERNDWEPPDKKVDTRKYRAEPKSIYEYQPGKSSVLTNEKMSRDISPEEIDLKNEPWYKFFSELEFGKPPPKKIWDYTPGDCSILPREDRKTNLDKDLSLCQTELEADLEKMETLNKAPSANVPQSSAISPTPEISSETPGYIYSSNFHAVKRESDGAPGDLTSLENERQIYKSVLEGGDIPLQGLSGLKRPSSSASTKDSESPRHFIPADYLESTEEFIRRRHDDKEKLLADQRRLKREQEEADIAARRHTGVIPTHHQFITNERFGDLLNIDDTAKRKSGSEMRPARAKFDFKAQTLKELPLQKGDIVYIYKQIDQNWYEGEHHGRVGIFPRTYIELLPPAEKAQPKKLTPVQVLEYGEAIAKFNFNGDTQVEMSFRKGERITLLRQVDENWYEGRIPGTSRQGIFPITYVDVIKRPLVKNPVDYMDLPFSSSPSRSATASPQFSSHSKLITPAPSSLPHSRRALSPEMHAVTSEWISLTVGVPGRRSLALTPPLPPLPEASIYNTDHLALSPRASPSLSLSLPHLSWSDRPTPRSVASPLALPSPHKTYSLAPTSQASLHMNGDGGVHTPSSGIHQDSFLQLPLGSSDSVISQLSDAFSSQSKRQPWREESGQYERKAERGAGERGPGGPKISKKSCLKPSDVVRCLSTEQRLSDLNTPEESRPGKPLGSAFPGSEAEQTERHRGGEQAGRKAARRGGSQQPQAQQRRVTPDRSQTSQDLFSYQALYSYIPQNDDELELRDGDIVDVMEKCDDGWFVGTSRRTKQFGTFPGNYVKPLYL a0a3l7ig96_crigr Tyrosine-protein kinase receptor CgPICR_013487 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MHLPNVPTVCPSLDIRSEVTELRRLENCSVVEGHLQILLMFAATGEDFRGLSFPRLTQVTDYLLLFRVYGLESLRDLFPNLAVIRGARLFLGYALIIFEMPHLRDVGLPSLGAVLRGAVRVEKNQELCHLSTIDWGLLQPVPGANHIVGNKLGEECADVCPGVLGAAGEPCARTTFSGHTDYRCWTSSHCQRVCPCPHGLACTPRGECCHSECLGGCSQPEDSRACVACRHLYFQGACLRACPPGTYQYESWRCVTAEVCAHLREVPGRTAAFGIYQGSCLAQCPPGFTRNGSSIFCHKCEGLCPKECKVGTKTIDSVQATQDLVGCTHVEGSLILNLRQGYNLEPELQRNLGLVETITGFLKIKHSFALVSLGFFKNLKLIRGDSMVDGNYTLYVLDNQNLQQLGSWVAAGLTIPVGKMYFAFNPRLCLEHIYQLEDVTGTRGRQSKAETDARQEHGQTRTLRFVFNLTEADRILLRWERYEPLEARDLLSFIVYYKESPFQNATEHVGPDACGTQSWNLLDVELPLSRTQEPGVTLAPLKPWTQYAVFVRAITLTTAEDSPHQGAQSPIVYLRTLPAAPTVPQDVISTSNSSSHLLVRWKPPVQRNGNVTYYLVLWQRLAEDGDLYINDYCHRGSLGVPQARGLRLPTSSHDTRFDREDPALEAEPEQGCCPCQHSPPGQALPTLETQEVTFQKKFENFLHHAITIPKSPWKVTSINKRPQRDSERHRREAGPLRQENNSSDFEIQEDKVPRERAVLSGLRHFTEYRIDIHACNHAAHTVGCSAATFVFARTMPHREADDIPGKVAWKAAGRSSVVLHWLEPPDPNGLILKYEIKYRRLGEEATVLCVSRLRYAKVGGVQLALLPPGNYSARVRATSLAGNGSWTDGVAFYITGPEEEDAGRLHILLSVTPVGFMLLIMLAALAFFYSKKRNSTLYTSVNPEYFSASHVYVPDEWEVPREQIAIIRELGQGSFGMVYEGLAQGLEAGEESTPVALKTVNELASARERIEFLKEASVMKAFKCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSHLRSLRPEAENNPGLPQPALSDMIQMAGEIADGMAYLAANKFVHRDLAARNCMVSQDFTVKIGDFGMTRDVYETDYYRKGGKGLLPVRWMAPESLKDGIFTTQSDVWSFGVVLWEIVTLAEQPYQGLSNEQVLKFVMDGGALEELEDCPLQLQELMRLCWQHSPRLRPTFVHILGRIQDELRPSFRLCSFYHSAECQRGQASSLSTETEPDSPPASNGAPDYRPPNGNPGH Similar to P15208 cbl-ship2 865542 n/a 865548 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 865549 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4 cbl-sorbs1-1 865542 n/a 865550 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 865549 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4 cbl-sorbs1-2 865542 n/a 865550 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 865549 unspecified role unspecified role bait bait n/a 865548 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4 insr_ship2-1 865542 n/a 865548 unspecified role unspecified role prey prey n/a 865551 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5 inppl1-sorbs1-1 865543 n/a 865550 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false n/a 865548 unspecified role unspecified role bait bait proline rich region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 865573 865571 physical association physical association false false false inppl1-sorbs1-2 865544 n/a 865548 unspecified role unspecified role bait bait proline rich region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 865550 unspecified role unspecified role prey prey physical association physical association false false false Figs. 1A, 1B inppl1-sorbs1-3 865545 n/a 865548 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 865550 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sh3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Figs. 1C inppl1-sorbs1-4 865546 n/a 865550 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 865548 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2 inppl1-sorbs1-5 865547 n/a 865548 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 865550 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3