IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mortensen-2002-3 Cell cycle-dependent assembly of a Gin4-septin complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mortensen EM., McDonald H., Yates J., Kellogg DR. kellogg@darwin.ucsc.edu Mol. Biol. Cell (1059-1524) 2002 Only protein-protein interactions imex curation mortensen-2002-1 Cell cycle-dependent assembly of a Gin4-septin complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Mortensen EM., McDonald H., Yates J., Kellogg DR. kellogg@darwin.ucsc.edu Mol. Biol. Cell (1059-1524) 2002 Only protein-protein interactions imex curation mortensen-2002-2 Cell cycle-dependent assembly of a Gin4-septin complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Mortensen EM., McDonald H., Yates J., Kellogg DR. kellogg@darwin.ucsc.edu Mol. Biol. Cell (1059-1524) 2002 Only protein-protein interactions imex curation mortensen-2002-4 Cell cycle-dependent assembly of a Gin4-septin complex. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mortensen EM., McDonald H., Yates J., Kellogg DR. Purified septin complex phosphorylated by Gin4.Component proteins then separated by SDS PAGE and identity of phosphorylated protein confirmed by western blot. kellogg@darwin.ucsc.edu Mol. Biol. Cell (1059-1524) 2002 Only protein-protein interactions imex curation nap1_yeast Nucleosome assembly protein NAP1 YKR048C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDPIRTKPKSSMQIDNAPTPHNTPASVLNPSYLKNGNPVRAQAQEQDDKIGTINEEDILANQPLLLQSIQDRLGSLVGQDSGYVGGLPKNVKEKLLSLKTLQSELFEVEKEFQVEMFELENKFLQKYKPIWEQRSRIISGQEQPKPEQIAKGQEIVESLNETELLVDEEEKAQNDSEEEQVKGIPSFWLTALENLPIVCDTITDRDAEVLEYLQDIGLEYLTDGRPGFKLLFRFDSSANPFFTNDILCKTYFYQKELGYSGDFIYDHAEGCEISWKDNAHNVTVDLEMRKQRNKTTKQVRTIEKITPIESFFNFFDPPKIQNEDQDEELEEDLEERLALDYSIGEQLKDKLIPRAVDWFTGAALEFEFEEDEEEADEDEDEEEDDDHGLEDDDGESAEEQDDFAGRPEQAPECKQS C0F97FBA1B9EEA83 cdc11_yeast Cell division control protein 11 CDC11 PSL9 YJR076C J1833 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGIIDASSALRKRKHLKRGITFTVMIVGQSGSGRSTFINTLCGQQVVDTSTTILLPTDTSTEIDLQLREETVELEDDEGVKIQLNIIDTPGFGDSLDNSPSFEIISDYIRHQYDEILLEESRVRRNPRFKDGRVHCCLYLINPTGHGLKEIDVEFIRQLGSLVNIIPVISKSDSLTRDELKLNKKLIMEDIDRWNLPIYNFPFDEDEISDEDYETNMYLRTLLPFAIIGSNEVYEMGGDVGTIRGRKYPWGILDVEDSSISDFVILRNALLISHLHDLKNYTHEILYERYRTEALSGESVAAESIRPNLTKLNGSSSSSTTTRRNTNPFKQSNNINNDVLNPASDMHGQSTGENNETYMTREEQIRLEEERLKAFEERVQQELLLKRQELLQREKELREIEARLEKEAKIKQEE 5CFFD4CD1F6C57D4 gin4_yeast Serine/threonine-protein kinase GIN4 GIN4 YDR507C D9719.13 Growth inhibitory protein 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAINGNSIPAIKDNTIGPWKLGETLGLGSTGKVQLARNGSTGQEAAVKVISKAVFNTGNVSGTSIVGSTTPDALPYGIEREIIIMKLLNHPNVLRLYDVWETNTDLYLVLEYAEKGELFNLLVERGPLPEHEAIRFFRQIIIGVSYCHALGIVHRDLKPENLLLDHKYNIKIADFGMAALETEGKLLETSCGSPHYAAPEIVSGIPYQGFASDVWSCGVILFALLTGRLPFDEEDGNIRTLLLKVQKGEFEMPSDDEISREAQDLIRKILTVDPERRIKTRDILKHPLLQKYPSIRDSKSIRGLPREDTYLTPLSESNSSIDATILQNLVILWHGRDPEGIKEKLREPGANAEKTLYALLYRFKCDTQKELIKQQQVKKRQSISSVSVSPSKKVSTTPQRRRNRESLISVTSSRKKPISFNKFTASSASSSNLTTPGSSKRLSKNFSSKKKLSTIVNQSSPTPASRNKRASVINVEKNQKRASIFSTTKKNKRSSRSIKRMSLIPSMKRESVTTKLMSTYAKLAEDDDWEYIEKETKRTSSNFATLIDEIFEYEKYEQIRKEKEELERKVREAKAREELERRRRKQEEKERARKLLEKEDLKRKQEELKKQIEIDISDLEQELSKHKEEKLDGNIRSISAPMENEEKNINHLEVDIDNILRRRNFSLQTRPVSRLDPGIMFSSPTEEVSPVEPKRTENERLTTEKKILETIRRSKFLGSSFNIDKELKLSKMEYPSIIAPQRLSEERVVSDSNDGYESLILPKDGNGVSQLKDSTATTAPVSDGRLRKISEIRVPQFTRKSRHFSESNKRLSVLSMYSTKESFTNLVDILKNGNLDVNNQQSQRIPTPRSADDSEFLFETVNEEAEYTGNSSNDERLYDVGDSTIKDKSALKLNFADRFNGSNEAKQTDNLHLPILPPLNGDNELRKQNSQEGDQAHPKIKSMIPESGSSSHTEKEEENEEKEEKKPEQHKQEEDQEKREKVVDDMEPPLNKSVQKIREKNAGSQAKDHSKDHLKEHKQDKNTAIGNGSFFRKFSKSSDKTMELYAKISAKQLFNGLEKLLRGWTQYGLKNIKSHPNNLTLTGKLSSDNIFSLRSTLFEVNIYPRGKMSVVQFKKVSGSFKAVKKLVNEVENVLNKEGVLQK EC16FF4BB49DD811 shs1_yeast Seventh homolog of septin 1 YDL225W Septation protein 7 SHS1 SEP7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTASTPPINLFRRKKEHKRGITYTMLLCGPAGTGKTAFANNLLETKIFPHKYQYGKSNASISSNPEVKVIAPTKVVSFNSKNGIPSYVSEFDPMRANLEPGITITSTSLELGGNKDQGKPEMNEDDTVFFNLIMTHGIGENLDDSLCSEEVMSYLEQQFDIVLAEETRIKRNPRFEDTRVHVALYFIEPTGHGLREVDVELMKSISKYTNVLPIITRADSFTKEELTQFRKNIMFDVERYNVPIYKFEVDPEDDDLESMEENQALASLQPFAIITSDTRDSEGRYVREYPWGIISIDDDKISDLKVLKNVLFGSHLQEFKDTTQNLLYENYRSEKLSSVANAEEIGPNSTKRQSNAPSLSNFASLISTGQFNSSQTLANNLRADTPRNQVSGNFKENEYEDNGEHDSAENEQEMSPVRQLGREIKQENENLIRSIKTESSPKFLNSPDLPERTKLRNISETVPYVLRHERILARQQKLEELEAQSAKELQKRIQELERKAHELKLREKLINQNKLNGSSSSINSLQQSTRSQIKKNDTYTDLASIASGRD BDA2631061A278F9 cdc12_yeast Cell division control protein 12 CDC12 CLA10 PSL7 YHR107C Septin protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAATATAAPVPPPVGISNLPNQRYKIVNEEGGTFTVMLCGESGLGKTTFINTLFQTVLKRADGQQHRQEPIRKTVEIDITRALLEEKHFELRVNVIDTPGFGDNVNNNKAWQPLVDFIDDQHDSYMRQEQQPYRTKKFDLRVHAVLYFIRPTGHGLKPIDIETMKRLSTRANLIPVIAKADTLTAQELQQFKSRIRQVIEAQEIRIFTPPLDADSKEDAKSGSNPDSAAVEHARQLIEAMPFAIVGSEKKFDNGQGTQVVARKYPWGLVEIENDSHCDFRKLRALLLRTYLLDLISTTQEMHYETYRRLRLEGHENTGEGNEDFTLPAIAPARKLSHNPRYKEEENALKKYFTDQVKAEEQRFRQWEQNIVNERIRLNGDLEEIQGKVKKLEEQVKSLQVKKSHLK A72DC393A6DBF924 cdc10_yeast Cell division control protein 10 CDC10 YCR2C YCR002C YCR022 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDPLSSVQPASYVGFDTITNQIEHRLLKKGFQFNIMVVGQSGLGKSTLINTLFASHLIDSATGDDISALPVTKTTEMKISTHTLVEDRVRLNINVIDTPGFGDFIDNSKAWEPIVKYIKEQHSQYLRKELTAQRERFITDTRVHAILYFLQPNGKELSRLDVEALKRLTEIANVIPVIGKSDTLTLDERTEFRELIQNEFEKYNFKIYPYDSEELTDEELELNRSVRSIIPFAVVGSENEIEINGETFRGRKTRWSAINVEDINQCDFVYLREFLIRTHLQDLIETTSYIHYEGFRARQLIALKENANSRSSAHMSSNAIQR 672467301D4D2697 bni5_yeast Bud neck protein 5 BNI5 N1706 YNL166C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGLDQDKIKKRLSQIEIDINQMNQMIDENLQLVEPAEDEAVEDNVKDTGVVDAVKVAETALFSGNDGADSNPGDSAQVEEHKTAQVHIPTENEANKSTDDPSQLSVTQPFIAKEQITHTAIAIGDSYNSFVANSAGNEKAKDSCTENKEDGTVNIDQNRGEADVEIIENNDDEWEDEKSDVEEGRVDKGTEENSEIESFKSPMPQNNTLGGENKLDAELVLDKFSSANKDLDIQPQTIVVGGDNEYNHESSRLADQTPHDDNSENCPNRSGGSTPLDSQTKIFIPKKNSKEDGTNINHFNSDGDGQKKMANFETRRPTNPFRVISVSSNSNSRNGSRKSSLNKYDSPVSSPITSASELGSIAKLEKRHDYLSMKCIKLQKEIDYLNKMNAQGSLSMEDGKRLHRAVVKLQEYLDKKTKEKYEVGVLLSRHLRKQIDRGENGQFWIGTK cdc3_yeast Cell division control protein 3 CDC3 L8543.7 YLR314C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLKEEQVSIKQDPEQEERQHDQFNDVQIKQESQDHDGVDSQYTNGTQNDDSERFEAAESDVKVEPGLGMGITSSQSEKGQVLPDQPEIKFIRRQINGYVGFANLPKQWHRRSIKNGFSFNLLCVGPDGIGKTTLMKTLFNNDDIEANLVKDYEEELANDQEEEEGQGEGHENQSQEQRHKVKIKSYESVIEENGVKLNLNVIDTEGFGDFLNNDQKSWDPIIKEIDSRFDQYLDAENKINRHSINDKRIHACLYFIEPTGHYLKPLDLKFMQSVYEKCNLIPVIAKSDILTDEEILSFKKTIMNQLIQSNIELFKPPIYSNDDAENSHLSERLFSSLPYAVIGSNDIVENYSGNQVRGRSYPWGVIEVDNDNHSDFNLLKNLLIKQFMEELKERTSKILYENYRSSKLAKLGIKQDNSVFKEFDPISKQQEEKTLHEAKLAKLEIEMKTVFQQKVSEKEKKLQKSETELFARHKEMKEKLTKQLKALEDKKKQLELSINSASPNVNHSPVPTKKKGFLR DCCD4C8C16AA5E25 gin4-nap1-2 Interaction between Gin4, Nap1 and Cdc11 demonstrated by coimmunoprecipitation 853860 n/a 853864 unspecified role unspecified role prey prey n/a 853865 unspecified role unspecified role prey prey n/a 853866 unspecified role unspecified role bait bait association association false false false Figs. 4A, 6A Cells were synchronised in G1 by treatment with alpha factor, temperature shift and the addition of dextrose-containing media with no effect on results. Loss of interaction was not caused by activation of an Swe1-dependent checkpoint. gin4-nap1-3 Interaction between Gin4 and Nap1 demonstrated by coimmunoprecipitation 853860 n/a 853864 unspecified role unspecified role prey prey n/a 853866 unspecified role unspecified role bait bait association association false false false Fig. 4A, 4C, 5B, 6A Interaction is not cell-cycle regulated and does not require Gin4 hyperphosphorylation. gin4-nap1-4 Interaction between Gin4, Nap1, Shs1 and Cdc11 demonstrated by coimmunoprecipitation 853860 n/a 853866 unspecified role unspecified role bait bait region (required) phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 853865 unspecified role unspecified role prey prey n/a 853864 unspecified role unspecified role prey prey n/a 853867 unspecified role unspecified role prey prey association association false false false Fig. 5B 1NM-PP1 - an inhibitor of CDC28 gin4-shs1-1 Interaction between Gin4 and Shs1 demonstrated by coimmunoprecipitation 853860 n/a 853866 unspecified role unspecified role bait bait Q12263:p.Lys48Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false n/a 853867 unspecified role unspecified role prey prey physical association physical association false false false Fig. 6C gin4-cdc3-1 Interactors of Gin4 853861 n/a 853866 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 853867 unspecified role unspecified role prey prey n/a 853868 unspecified role unspecified role prey prey n/a 853864 unspecified role unspecified role prey prey n/a 853865 unspecified role unspecified role prey prey n/a 853869 unspecified role unspecified role prey prey n/a 853870 unspecified role unspecified role prey prey n/a 853871 unspecified role unspecified role prey prey association association false false false Fig. 3A, Table 1 Single peptides for Ctf7 (P43605), YJR083C (P47129), and YLL054C (Q12244) were identified my mass spec but could not be observed by Coomassie Blue staining, other proteins were present in both te control and pulldown column. shs1-cdc3-1 Interactors of Shs1 853861 n/a 853871 unspecified role unspecified role prey prey n/a 853868 unspecified role unspecified role prey prey n/a 853865 unspecified role unspecified role prey prey n/a 853869 unspecified role unspecified role prey prey n/a 853867 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 8A Column washed with 1M KCl gin4-nap1-1 Interactors of Gin4 853862 n/a 853866 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 853867 unspecified role unspecified role prey prey n/a 853864 unspecified role unspecified role prey prey n/a 853865 unspecified role unspecified role prey prey association association false false false Fig. 3B gin4-shs1-2 Phosphorylation of Shs1 by Gin4 853863 n/a 853866 enzyme enzyme neutral component neutral component Q12263:p.Lys48Ala Alters kinetics of interaction mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 853867 enzyme target enzyme target substrate substrate neutral component neutral component yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast phosphorylation phosphorylation reaction phosphorylation false false false Figs. 8B and 8C