UniProt UniProt http://www.uniprot.org moreno_borc-2001-2 Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Moreno-Borchart AC., Strasser K., Finkbeiner MG., Shevchenko A., Shevchenko A., Knop M. michael.knop@embl-hiedelberg.de EMBO J. (0261-4189) 2001 Only protein-protein interactions imex curation moreno_borc-2001-3 Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Moreno-Borchart AC., Strasser K., Finkbeiner MG., Shevchenko A., Shevchenko A., Knop M. michael.knop@embl-hiedelberg.de EMBO J. (0261-4189) 2001 Only protein-protein interactions imex curation moreno_borc-2001-1 Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down ms participant Identification by mass spectrometry ms participant Moreno-Borchart AC., Strasser K., Finkbeiner MG., Shevchenko A., Shevchenko A., Knop M. michael.knop@embl-hiedelberg.de EMBO J. (0261-4189) 2001 Only protein-protein interactions imex curation mpc54_yeast Meiotic plaque component protein 54 MPC54 YOR177C protein protein yeast Saccharomyces cerevisiae Baker's yeast MPEDTSYSNSFEDYYNNSHAISPYKDSFYKEMTPSKPNVRFGDDDVNIFDQRKKVNEINKNNTVKRSIPSSISTTITPNKSSLKSPRGKRASKNSFDNETKLESKNETLKEVNDAVNRCYALCNIPTKHVSINSISDLAQTFETLAVGITHETNRKAECERSKNAIDSLYYHEQLEKKELNEKSLQMAIDHLLKVTKQNLRQADDGNKLKETEALKSFIEEIEEVDDNKISINSLQQQLLEEKTANNILRRDYYKLQERGRRLCHEFQELQDDYSKQMKQKEYEVQKLKNEIKVLLNMNDNLKAEKAHYSQKEKQYFQKYTYIEKYMNHVKEEYNRKEDECKKLNFIIDKSMKKIEHLERSLQTQFTAQNSFSTAMIQEEGPKDAHLKDRYHKVKEFMEQKLQTSKINDPSCSEAEALDNVLCLIESSMKTLDKNSKCYPIATKKCIKYVTDSPRLKENEHVTN 74C614223B385CAC ady3_yeast Accumulates dyads protein 3 YDL239C ADY3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNHWLAFLNKPESLKEQNSDCDQQGEMRHVTDGTLTKSPESKPFRERRSQTWIDSEVPTSTEKSNVQESISSDIISKLSNRRSRRNRSESWAGSEASSPSGNISTLENATEKNTLKSPNKFLQRGGLPTVGIGSQALSPAGKPSTLGNVSPGKFTTYKVHNSIEVNRFSSTPTKLLTNPHKVAAISNDEHYVVSNESLEENIEVAHLENVFRSSKTPDEEQSEYMKLGEIRLSSSSYGGSISKENSLPKVLDELQSQNEEIKALRQKLEEKDDRIQELEELNSMNDAKLQRIEDLQKEFHNERKAASKRLNIVQDRFRKEIKKIREEKITDFQNKNASKKEKNEVTSAKTKCKAFSQRNILVSELYRKQKQILNLQQENDKFLKDINESNNSIVKLRSEVEILKSNLQLSQDENKKLHDNGSFYEKRLNDVYSYMQNLSLFEKDLGKFILEEMKCGHSPSMFQNGFAKLYPDFQDIKNLENMEQYKQLKGKIELLEKNDRIRLEKIISVFKLINERLHFMQQQHSHKIKYLQKEALTKEQQFRLEKRRWHDILNLKEENFQKLKSELKEKLILSEKIQKNAEDKLNDYMNEHQEIVEKLQNQALIASRWSTQIQESENTHKKITDELAGKQSEILKLEETILSLKEDVFQEKLNLKKLYGDPSTELNFETVGKSFPHITKEKYDSLGLDILTDLTYVQSQNLIKNLLIVLDIPLKTFLKIVPTIVIQLRCELTLLTKFANDLNLKVFGKQLDFKSRRKVAMNEFLNNHDIAEVKHPLEYDLQALFKYFFS 46F17474F30FD3D9 mpc70_yeast Sporulation-specific protein 21 Meiotic plaque component protein 70 SPO21 MPC70 YOL091W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNILKASNMEGTSTMTVTSRSSEDSSCISNHEQDTDTHKDGDTSGLENSKISKRKWMKEFFKLSKSPASKSSRSIGSMKSNQSLVSMKSSDDGNSYKNDYSSICGNSLPSAGLSRSNSVKELKLDSTGSQRSKNNVAMLARSSTTSQTTCSSSSSSSSYNSIKGNENDILLQNNNHFRHNKEIPQSKGSSNINTASIMSQYNVDTQATAIMSDMQKQYDSQQMTSPFVNEDLHFDPNGEVSHVIKAIFKEIGYKYDDFSDIPVFQLMQEMYQLVKKNSSARRTKITDYASKLKEKEAQLKSQNDKILKLETTNKAYKTKYKEVSLENKKIKEAFKELDNESYNHDEELLKKYKYTRETLDRVNREQQLIIDQNEFLKKSVNELQNEVNATNFKFSLFKEKYAKLADSITELNTSTKKREALGENLTFECNELKEICLKYKKNIENISNTNKNLQNSFKNERKKVLDLRNERNLLKKEILLIECHGSYSLLLVSNILTCYRFLLPSDTIIETESLIKELLNMNNSLSNHVSSSDEPPAEYSKRLELKCVEFEEKLLYFYQELVTKKIIDVIYKCFINYYKKSRQTDQKSNQNSSTPYKQSQRQVPHSIK E216B0C9B865B412 nud1_yeast Protein NUD1 YOR373W NUD1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDMDTQEAELSSQLENLTINSPRKLRSNAHSNSGKVFKEYESNHDFQDSNFTSQVVEPAISDSVKKPPTMTVLNNYSTVHQKVPSGFSGTTATSHQEAQWKQYFPGIGSGGGTNFGGAVGTANKVPESDLIVSDLVKDLSGVLETNTFKRHLDMKNKTTTMQTHENHDTISISHSKDFFNAEKVSSSFSDDSDSGPAAEAHDVFDGILQKQKSNYLVGSYPSNSNNKNNNNNNNNNNNNSININNKDNARTKEEDEEDTSNSFEFSSSSSMSSSQTQSGRKSKVLKKPPLNTISPGQLGYQFNHTHGAWDPPLNQGLDVSSSHSLDNTSSNQSQFATMVPTGDNHTNGKAPSILDKKAYELTSTKPGDVGYRQKKIQEEENLANSDDTPLDTPKFNDLFTKNGTRAKVKGQMRTSRSISNSNLLEAHKKLKTFPAERVEDITSISEVNTSFNETEKQLISILTSKLSGSPSYDSDWEKILKVDLSRGKLKNMFGMQRLLPNVLVLNLSDNEMNTLEGIPSNVVQLFCSNNKITSAHCSLAGFHDLECLDLSYNLLNTSLKFLSLCHHLQEVNLSYNSIQSLEGIGSSRMKKLNLSNNEINGIIDFEQLILTNNSVVGGWLTVEVLDLSNNNIIGVRNINCLPRLKVLNLNGNPLVSIVESSKMENGTLRALSIKNTGGALSKLQNYKLDDQFTFPYQNLKILKLDGFAQLSKWQKWPATLQILEINGGLASSLPRFSSLKSTNLYSLTIANVRDFTHLPVDLSKELPFLQELHLPGNNLQNAHKLTKTLPRQSVKFLDLRNNPITTPRHDRASTSLHYRQLLQLAGLCQQQCPALATLWLDDTPAPTATNL cnm67_yeast Chaotic nuclear migration protein 67 CNM67 N1264 YNL225C protein protein yeast Saccharomyces cerevisiae Baker's yeast MTDFDLMNFPFHERLDSPVSENGEIKDGEPIPQNWLNENHVGKSILPLFVNPEDVINCNFSNARDSYEENKSPSMDQMNYARNTSYQESPGLQERPKNEKDKSPIGTDVHKKDVPNFIHSTPRENSSKHFTRANEQASAQPTDEHTSPDISIEDCNGAKIFLQNSLSKEDFRMLENVILGYQKKVIELGRDNLRQEERANSLQKELEAATKSNDKTLDNKKKIEEQTVLIENLTKDLSLNKEMLEKANDTIQTKHTALLSLTDSLRKAELFEIPIGILFFDLYDSEENSSKLDHILQEKYPNIKGFLCASQQEELSRISQRFKNAKAEAEDLRNELENKKIEIQTMREKNNTLIGTNKTLSKQNKILCDKFDKLTIDEKEILKGCNEEIKIKLERLNERLGSWEKSKEKYETSLKDKEKMLADAEKKTNTLSKELDNLRSRFGNLEGNTSERITIKNILQSRPDISAEECNFLMVEQIDSANLTTLQNTVKEIVLAVGIPYPKLRRKIPLLAIKLKYENIMLSNFAQRLHRQVYSQEMNLKKFTDQAYYDFMSTRRMDSIDHHLERCLDHLYDHILEKMVK C4DE7631A70E695A ssp1_yeast Sporulation-specific protein 1 SSP1 YHR184W protein protein yeast Saccharomyces cerevisiae Baker's yeast MRSSGTYENDPSGEITSTSPKQSKQKKPTKFRERMRRWLQNGKNNNHQGEEDVPEIFNKNFYPQTGMTAFNNNDNGEVQDVTNNFFLPSEDESGPVQSSVKTFLTGNNDEDSNFQQNQNPKQKSELPKSPYRQKPTQEIALLKDLFVTNKYDDPYLNSSTRFGNITSTFPSSLSLRTVTLQTIKKRIDCISAKKKEVWKTEEKFLKDILMWLQSSNFEDPDTISLIHEIEKIFEEDIHFEQNVSDCLKEISNNFEYICMRETQLINEGNILKNDLKKYAKAREHKGEKHEDTEVLREKVISSQKSFDVTKRHYKHAISITTRQLFMNLAFEYYENCSDMKDISRKYLQESLSTLQTIDTLSFSEELEKIRKRRFDKFWAKTNPDPTNNIQKFVNMRTGVAGFNDSLMNHLYGKLSFGVAPVEEELQNSQPEHTDVPENVWNEVLSDYNSMDGNPITSNKFLSAKELEPDQLVELLAQEKEEKEAKNISSSTAEVPSISQPEIKKENLESNDSLILRSTKRNVNVNAASLRNLSIKKTQVKPESASEESKVLAAALNDAKQNLDENVWRTPI A199EB4159771A03 don1_yeast Donuts protein 1 DON1 D9954.4 YDR273W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKKNRKGKENNAAKTSFLKVENIKNTNSGLELPSQDYTNVEEKESSPKTDFPLITKEHVNTKTDSNILDYPTIGDLVSSVEKLCVLKELKIAFPEVDDTLIKAILIASQGVLEPAFNSLLYYSSPEENTDFALPMKPISVEDYSKINVSEILQREIFDDIEDEFSGQGINGSMVISKIESELSSLAEHIGNISTPGSNREVAESTRNVAVAEGHNTILSNEDSILKGKEKGKEEEKEKGEEKGVNSLKGAAVKVVAKSLKNNRIPVTVKRNEPSNNLFDVLNCDESEEEEEQDVETNTSNQERKNQGGNTEVPEAQRDSADRLPAKDDGGYKSAFGTDSCGLFAADAKDEKKQVHPSRQELSFT 6E13542050862709 sso1_yeast Protein SSO1 SSO1 P1405 YPL232W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSYNNPYQLETPFEESYELDEGSSAIGAEGHDFVGFMNKISQINRDLDKYDHTINQVDSLHKRLLTEVNEEQASHLRHSLDNFVAQATDLQFKLKNEIKSAQRDGIHDTNKQAQAENSRQRFLKLIQDYRIVDSNYKEENKEQAKRQYMIIQPEATEDEVEAAISDVGGQQIFSQALLNANRRGEAKTALAEVQARHQELLKLEKSMAELTQLFNDMEELVIEQQENVDVIDKNVEDAQLDVEQGVGHTDKAVKSARKARKNKIRCWLIVFAIIVVVVVVVVVPAVVKTR 6E4C6D35C3CD9D94 sso2_yeast Protein SSO2 SSO2 YM8010.13C YMR183C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNANPYENNNPYAENYEMQEDLNNAPTGHSDGSDDFVAFMNKINSINANLSRYENIINQIDAQHKDLLTQVSEEQEMELRRSLDDYISQATDLQYQLKADIKDAQRDGLHDSNKQAQAENCRQKFLKLIQDYRIIDSNYKEESKEQAKRQYTIIQPEATDEEVEAAINDVNGQQIFSQALLNANRRGEAKTALAEVQARHQELLKLEKTMAELTQLFNDMEELVIEQQENVDVIDKNVEDAQQDVEQGVGHTNKAVKSARKARKNKIRCLIICFIIFAIVVVVVVVPSVVETRK mpc54-ady3-2 787289 n/a 787292 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 787293 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1B ady3-mpc54-1 787289 n/a 787292 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 787293 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1B mpc70-ady3-1 787289 n/a 787294 unspecified role unspecified role bait bait n/a 787293 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1B ady3-mpc70-1 787289 n/a 787294 unspecified role unspecified role prey prey n/a 787293 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1B ady3-nud1-1 787289 n/a 787295 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 787293 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1B ady3-cnm67-1 787289 n/a 787296 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 787293 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1B ady3-ssp1-1 787289 n/a 787293 unspecified role unspecified role bait bait n/a 787297 unspecified role unspecified role prey prey physical association physical association false false false Fig 1B ady3-ady3-1 787289 n/a 787293 unspecified role unspecified role bait bait n/a 787293 unspecified role unspecified role prey prey physical association physical association false false false Fig 1C don1-don1-1 787289 n/a 787298 unspecified role unspecified role prey prey n/a 787298 unspecified role unspecified role bait bait physical association physical association false false false Fig 1C ssp1-ssp1-1 787289 n/a 787297 unspecified role unspecified role bait bait n/a 787297 unspecified role unspecified role prey prey physical association physical association false false false Fig 1C ady3-don1-2 787290 n/a 787293 unspecified role unspecified role neutral component neutral component n/a 787298 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 3A ssp1-don1-1 787290 n/a 787298 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 787297 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 3C, 3D sso1-don1-1 787290 n/a 787299 unspecified role unspecified role neutral component neutral component n/a 787300 unspecified role unspecified role neutral component neutral component n/a 787298 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 6 The antibody used recognizes both SSO1 and SSO2. The interaction requires SSP1. mpc54-ady3-1 787291 n/a 787293 unspecified role unspecified role prey prey n/a 787292 unspecified role unspecified role bait bait c-terminus protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 1A