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    <entry>
        <source releaseDate="2013-09-02+01:00">
            <names>
                <shortLabel>MINT</shortLabel>
                <fullName>MINT, Dpt of Biology, University of Rome Tor Vergata</fullName>
                <alias typeAc="MI:1041" type="synonym">MINT</alias>
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            <attributeList>
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                <attribute name="url" nameAc="MI:0614">url&amp;gt;http://mint.bio.uniroma2.it/mint/</attribute>
                <attribute name="url" nameAc="MI:0614">https://mint.bio.uniroma2.it</attribute>
                <attribute name="id-validation-regexp" nameAc="MI:0628">MINT-[0-9]+</attribute>
                <attribute name="search-url" nameAc="MI:0615">https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac}</attribute>
                <attribute name="definition">The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules.</attribute>
                <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it</attribute>
                <attribute name="url">http://mint.bio.uniroma2.it/mint</attribute>
            </attributeList>
        </source>
        <experimentList>
            <experimentDescription id="793673">
                <names>
                    <shortLabel>skiba-2000-1</shortLabel>
                    <fullName>The effector enzyme regulates the duration of G protein signaling in vertebrate photoreceptors by increasing the affinity between transducin and RGS protein.</fullName>
                </names>
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                <hostOrganismList>
                    <hostOrganism ncbiTaxId="-1">
                        <names>
                            <shortLabel>in vitro</shortLabel>
                            <fullName>In vitro</fullName>
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                    <names>
                        <shortLabel>pull down</shortLabel>
                        <fullName>pull down</fullName>
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                </interactionDetectionMethod>
                <participantIdentificationMethod>
                    <names>
                        <shortLabel>predetermined</shortLabel>
                        <fullName>predetermined participant</fullName>
                        <alias typeAc="MI:1041" type="synonym">predetermined</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
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                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
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                </participantIdentificationMethod>
                <attributeList>
                    <attribute name="author-list" nameAc="MI:0636">Skiba NP., Hopp JA., Arshavsky VY.</attribute>
                    <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
                    <attribute name="publication year" nameAc="MI:0886">2000</attribute>
                </attributeList>
            </experimentDescription>
        </experimentList>
        <interactorList>
            <interactor id="793674">
                <names>
                    <shortLabel>rgs9_bovin</shortLabel>
                    <fullName>Regulator of G-protein signaling 9</fullName>
                    <alias typeAc="MI:0301" type="gene name">RGS9</alias>
                </names>
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                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
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                </interactorType>
                <organism ncbiTaxId="9913">
                    <names>
                        <shortLabel>bovin</shortLabel>
                        <fullName>Bos taurus (Bovine)</fullName>
                        <alias typeAc="MI:1041" type="synonym">Bovine</alias>
                    </names>
                </organism>
                <sequence>MTIRHQGQQYRPRMAFLRKIEALVKDMQDPDTGVRVQNQKVKVVSIPHAMTGSDVLQWISQRLWISGLEAQNLGNFIVKYGYIYPLQDPRNLTLKPDSSLYRFQTPYFWPTQQWPAEDVDYAIYLAKRNIKKKGILEEYEKENYNFLNKKINYKWDFVIMQAREQYRAGKERNKVDRCALDCQEKAYWLVHRCPPGANNVLDYGLDRVTNPNEDQKQTVVSVRKEIMYYRQALMRSTVKSSVSLGGIVKYSEQFSSNDAIMSGCLPSNPWITDDTQFWDLNAKLVDIPTKMRVERWAFNFSELIRDPKGRQSFQHFLRKEFSGENLGFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGKTMDITVKGLKHPHRYVLDAAQTHIYMLMKKDSYARYLKSPIYKEMLAKAIEPQGTTRKSSSLPFMRRHLRSSPSPVILRQLEEEAKAREAATTVDITQVMSKLDRRSQLRKEPPPK</sequence>
                <attributeList>
                    <attribute name="function" nameAc="MI:0613">Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction</attribute>
                    <attribute name="comment" nameAc="MI:0612">mint</attribute>
                </attributeList>
            </interactor>
            <interactor id="793675">
                <names>
                    <shortLabel>gnat1_bovin</shortLabel>
                    <fullName>Guanine nucleotide-binding protein G(t) subunit alpha-1</fullName>
                    <alias typeAc="MI:0301" type="gene name">GNAT1</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Transducin alpha-1 chain</alias>
                </names>
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                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0001917" secondary="photoreceptor inner segment" version="SP_197"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
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                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
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                    </xref>
                </interactorType>
                <organism ncbiTaxId="9913">
                    <names>
                        <shortLabel>bovin</shortLabel>
                        <fullName>Bos taurus (Bovine)</fullName>
                        <alias typeAc="MI:1041" type="synonym">Bovine</alias>
                    </names>
                </organism>
                <sequence>MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFDRASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFSEKIKKAHLSICFPDYNGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF</sequence>
                <attributeList>
                    <attribute name="function" nameAc="MI:0613">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase</attribute>
                    <attribute name="comment" nameAc="MI:0612">mint</attribute>
                </attributeList>
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        </interactorList>
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                    <shortLabel>gnat1-rgs9</shortLabel>
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                </xref>
                <experimentList>
                    <experimentRef>793673</experimentRef>
                </experimentList>
                <participantList>
                    <participant id="793677">
                        <names>
                            <shortLabel>rgs9_bovin</shortLabel>
                            <alias typeAc="MI:0345" type="author assigned name">RGS9</alias>
                        </names>
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                            <secondaryRef db="uniprotkb" dbAc="MI:0486" id="O46469" secondary="O46469" refType="source reference" refTypeAc="MI:0685"/>
                            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7381621" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>793674</interactorRef>
                        <participantIdentificationMethodList>
                            <participantIdentificationMethod>
                                <names>
                                    <shortLabel>western blot</shortLabel>
                                    <fullName>western blot</fullName>
                                    <alias typeAc="MI:0303" type="go synonym">Immuno blot</alias>
                                    <alias typeAc="MI:1041" type="synonym">Immuno blot</alias>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-973" refType="identity" refTypeAc="MI:0356"/>
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                                </xref>
                            </participantIdentificationMethod>
                        </participantIdentificationMethodList>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>unspecified role</shortLabel>
                                    <fullName>unspecified role</fullName>
                                </names>
                                <xref>
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                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                        <experimentalPreparationList>
                            <experimentalPreparation>
                                <names>
                                    <shortLabel>cell lysate</shortLabel>
                                    <fullName>cell lysate</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0344" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537757" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalPreparation>
                            <experimentalPreparation>
                                <names>
                                    <shortLabel>physiological level</shortLabel>
                                    <fullName>physiological level</fullName>
                                    <alias typeAc="MI:0303" type="go synonym">endogenous level</alias>
                                    <alias typeAc="MI:0303" type="go synonym">endogenous</alias>
                                    <alias typeAc="MI:1041" type="synonym">endogenous</alias>
                                    <alias typeAc="MI:1041" type="synonym">endogenous level</alias>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0222" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537793" refType="identity" refTypeAc="MI:0356"/>
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                                </xref>
                            </experimentalPreparation>
                        </experimentalPreparationList>
                    </participant>
                    <participant id="793678">
                        <names>
                            <shortLabel>gnat1_bovin</shortLabel>
                            <alias typeAc="MI:0345" type="author assigned name">transducin</alias>
                        </names>
                        <xref>
                            <primaryRef db="mint" dbAc="MI:0471" id="MINT-6825847" refType="identity" refTypeAc="MI:0356"/>
                            <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P04695" secondary="P04695" refType="source reference" refTypeAc="MI:0685"/>
                            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7381617" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>793675</interactorRef>
                        <participantIdentificationMethodList>
                            <participantIdentificationMethod>
                                <names>
                                    <shortLabel>predetermined</shortLabel>
                                    <fullName>predetermined participant</fullName>
                                    <alias typeAc="MI:1041" type="synonym">predetermined</alias>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </participantIdentificationMethod>
                        </participantIdentificationMethodList>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>unspecified role</shortLabel>
                                    <fullName>unspecified role</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                        <experimentalPreparationList>
                            <experimentalPreparation>
                                <names>
                                    <shortLabel>purified</shortLabel>
                                    <fullName>purified</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalPreparation>
                        </experimentalPreparationList>
                        <featureList>
                            <feature id="793679">
                                <names>
                                    <shortLabel>his tagged</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="mint" dbAc="MI:0471" id="MINT-6825850" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7381625" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>his tag</shortLabel>
                                        <fullName>his tag</fullName>
                                        <alias typeAc="MI:0303" type="go synonym">Hexa-His-tag</alias>
                                        <alias typeAc="MI:0303" type="go synonym">6-His-tag</alias>
                                        <alias typeAc="MI:0303" type="go synonym">Histidine-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">Hexa-His-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">Histidine-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">6-His-tag</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </startStatus>
                                        <endStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </endStatus>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                        </featureList>
                    </participant>
                </participantList>
                <interactionType>
                    <names>
                        <shortLabel>physical association</shortLabel>
                        <fullName>physical association</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactionType>
                <modelled>false</modelled>
                <intraMolecular>false</intraMolecular>
                <negative>false</negative>
                <attributeList>
                    <attribute name="figure legend" nameAc="MI:0599">F4</attribute>
                    <attribute name="comment" nameAc="MI:0612">mint</attribute>
                </attributeList>
            </interaction>
        </interactionList>
    </entry>
</entrySet>
