IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gable-2000-1 Tsc3p is an 80-amino acid protein associated with serine palmitoyltransferase and required for optimal enzyme activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gable K., Slife H., Bacikova D., Monaghan E., Dunn TM. Coimmunoprecipitations performed in microsomes solubilized with 2 mM sucrose monolaurate Tdunn@usuhs.mil J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation gable-2000-2 Tsc3p is an 80-amino acid protein associated with serine palmitoyltransferase and required for optimal enzyme activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gable K., Slife H., Bacikova D., Monaghan E., Dunn TM. Although Lcb1 and Lcb2 were separately shown to bind to Tsc3, the author has shown that the two proteins are unstable in the absence of their partner, so infers that all 3 proteins must be present in each coimmunoprecipitation. The data has been annotated to reflect this. Coimmunoprecipitations performed in microsomes solubilized with 2 mM sucrose monolaurate Tdunn@usuhs.mil J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation gable-2000-3 Tsc3p is an 80-amino acid protein associated with serine palmitoyltransferase and required for optimal enzyme activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gable K., Slife H., Bacikova D., Monaghan E., Dunn TM. Coimmunoprecipitations performed in microsomes solubilized with 2 mM sucrose monolaurate Tdunn@usuhs.mil J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation lcb2_yeast Serine palmitoyltransferase 2 LCB2 SCS1 YDR062W YD9609.16 D4246 TSC1 Long chain base biosynthesis protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTPANYTRVPLCEPEELPDDIQKENEYGTLDSPGHLYQVKSRHGKPLPEPVVDTPPYYISLLTYLNYLILIILGHVHDFLGMTFQKNKHLDLLEHDGLAPWFSNFESFYVRRIKMRIDDCFSRPTTGVPGRFIRCIDRISHNINEYFTYSGAVYPCMNLSSYNYLGFAQSKGQCTDAALESVDKYSIQSGGPRAQIGTTDLHIKAEKLVARFIGKEDALVFSMGYGTNANLFNAFLDKKCLVISDELNHTSIRTGVRLSGAAVRTFKHGDMVGLEKLIREQIVLGQPKTNRPWKKILICAEGLFSMEGTLCNLPKLVELKKKYKCYLFIDEAHSIGAMGPTGRGVCEIFGVDPKDVDILMGTFTKSFGAAGGYIAADQWIIDRLRLDLTTVSYSESMPAPVLAQTISSLQTISGEICPGQGTERLQRIAFNSRYLRLALQRLGFIVYGVADSPVIPLLLYCPSKMPAFSRMMLQRRIAVVVVAYPATPLIESRVRFCMSASLTKEDIDYLLRHVSEVGDKLNLKSNSGKSSYDGKRQRWDIEEVIRRTPEDCKDDKYFVN 06DF4A709CC9F29B lcb1_yeast Serine palmitoyltransferase 1 YMR296C LCB1 END8 TSC2 Long chain base biosynthesis protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAHIPEVLPKSIPIPAFIVTTSSYLWYYFNLVLTQIPGGQFIVSYIKKSHHDDPYRTTVEIGLILYGIIYYLSKPQQKKSLQAQKPNLSPQEIDALIEDWEPEPLVDPSATDEQSWRVAKTPVTMEMPIQNHITITRNNLQEKYTNVFNLASNNFLQLSATEPVKEVVKTTIKNYGVGACGPAGFYGNQDVHYTLEYDLAQFFGTQGSVLYGQDFCAAPSVLPAFTKRGDVIVADDQVSLPVQNALQLSRSTVYYFNHNDMNSLECLLNELTEQEKLEKLPAIPRKFIVTEGIFHNSGDLAPLPELTKLKNKYKFRLFVDETFSIGVLGATGRGLSEHFNMDRATAIDITVGSMATALGSTGGFVLGDSVMCLHQRIGSNAYCFSACLPAYTVTSVSKVLKLMDSNNDAVQTLQKLSKSLHDSFASDDSLRSYVIVTSSPVSAVLHLQLTPAYRSRKFGYTCEQLFETMSALQKKSQTNKFIEPYEEEEKFLQSIVDHALINYNVLITRNTIVLKQETLPIVPSLKICCNAAMSPEELKNACESVKQSILACCQESNK 9F7F93E4B2C70FDB tsc3_yeast Serine palmitoyltransferase-regulating protein TSC3 TSC3 Temperature-sensitive CSG2-mutant suppressor protein 3 YBR058C-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MTQHKSSMVYIPTTKEAKRRNGKSEGILNTIEEVVEKLYWTYYIHLPFYLMASFDSFFLHVFFLTIFSLSFFGILKYCFL F23EE19603FEC87E lcb1-lcb2-1 Interaction between lcb1 and lcb2 identified by coimmunoprecipitation 789500 n/a 789503 unspecified role unspecified role prey prey n/a 789504 unspecified role unspecified role bait bait physical association physical association false false false Figs. 5A and 5B Interaction does not require the presence of Tsc3 lcb1-lcb2-2 Interaction between lcb1 and lcb2 identified by coimmunoprecipitation 789500 n/a 789503 unspecified role unspecified role bait bait n/a 789504 unspecified role unspecified role prey prey physical association physical association false false false Figs. 5A and 5B Interaction does not require the presence of Tsc3 lcb1-lcb2-3 Interaction between lcb1 and lcb2 identified by coimmunoprecipitation 789500 n/a 789504 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 789503 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5A Tagged protein was expressed in cells lacking lcb1 lcb1-lcb2-4 Interaction between lcb1 and lcb2 identified by coimmunoprecipitation 789500 n/a 789503 unspecified role unspecified role prey prey n/a 789504 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5A Tagged protein was expressed in cells lacking lcb1 lcb1-lcb2-5 Interaction between lcb1, lcb2 and Tsc3 identified by coimmunoprecipitation 789501 n/a 789503 unspecified role unspecified role bait bait n/a 789505 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 789504 unspecified role unspecified role prey prey association association false false false Fig. 6C Tsc3 is lost from this protein complex at high salt concentrations lcb1-lcb2-6 Interaction between lcb1, lcb2 and Tsc3 identified by coimmunoprecipitation 789501 n/a 789504 unspecified role unspecified role bait bait n/a 789505 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 789503 unspecified role unspecified role prey prey association association false false false Fig. 6C Tsc3 is lost from this protein complex at high salt concentrations lcb1-lcb2-7 Interaction between lcb1, lcb2 and Tsc3 identified by coimmunoprecipitation 789502 n/a 789505 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 789503 unspecified role unspecified role prey prey n/a 789504 unspecified role unspecified role prey prey association association false false false Fig. 6D