IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ golovkin-1998-1 The plant U1 small nuclear ribonucleoprotein particle 70K protein interacts with two novel serine/arginine-rich proteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Golovkin M., Reddy AS. reddy@lamar.colostate.edu cDNA library of 3 day old etiolated Arabidopsis seedlings Plant Cell (1040-4651) 1998 golovkin-1998-2 The plant U1 small nuclear ribonucleoprotein particle 70K protein interacts with two novel serine/arginine-rich proteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Golovkin M., Reddy AS. reddy@lamar.colostate.edu cDNA library of 3 day old etiolated Arabidopsis seedlings Plant Cell (1040-4651) 1998 golovkin-1998-3 The plant U1 small nuclear ribonucleoprotein particle 70K protein interacts with two novel serine/arginine-rich proteins. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Golovkin M., Reddy AS. reddy@lamar.colostate.edu cDNA library of 3 day old etiolated Arabidopsis seedlings Plant Cell (1040-4651) 1998 rsz22_arath Serine/arginine-rich splicing factor RSZ22 RSZ22 RS-containing zinc finger protein 22 RSZP22 SRZ22 F28M20.230 At4g31580 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSRVYVGNLDPRVTERELEDEFRAFGVVRSVWVARRPPGYAFLDFEDPRDARDAIRALDGKNGWRVEQSHNRGERGGGGRGGDRGGGGGGRGGRGGSDLKCYECGETGHFARECRNRGGTGRRRSKSRSRTPPRYRRSPSYGRRSYSPRARSPPPPRRRSPSPPPARGRSYSRSPPPYRAREEVPYANGNGLKERRRSRS ru17_arath U1 small nuclear ribonucleoprotein 70 kDa RNU1 T3A5.50 At3g50670 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGDSGDPFLRNPNAAVQARAKVQNRANVLQLKLMGQSHPTGLTNNLLKLFEPRPPLEYKPPPEKRKCPPYTGMAQFVSNFAEPGDPEYAPPKPEVELPSQKRERIHKLRLEKGVEKAAEDLKKYDPNNDPNATGDPYKTLFVSRLNYESSESKIKREFESYGPIKRVHLVTDQLTNKPKGYAFIEYMHTRDMKAAYKQADGQKIDGRRVLVDVERGRTVPNWRPRRLGGGLGTSRVGGGEEIVGEQQPQGRTSQSEEPSRPREEREKSREKGKERERSRELSHEQPRERSRDRPREDKHHRDRDQGGRDRDRDSRRDRDRTRDRGDRDRRDRDRGRDRTSRDHDRDRSRKKERDYEGGEYEHEGGGRSRERDAEYRGEPEETRGYYEDDQGDTDRYSHRYDKMEEDDFRYEREYKRSKRSESREYVR rsz21_arath Serine/arginine-rich splicing factor RSZ21 RSZ21 RS-containing zinc finger protein 21 RSZP21 SRZ21 T23E23.2 At1g23860 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTRVYVGNLDPRVTERELEDEFKAFGVLRNVWVARRPPGYAFLEFDDERDALDAISALDRKNGWRVELSHKDKGGRGGGGGRRGGIEDSKCYECGELGHFARECRRGRGSVRRRSPSPRRRRSPDYGYARRSISPRGRRSPPRRRSVTPPRRGRSYSRSPPYRGSRRDSPRRRDSPYGRRSPYANGV rnu1-srz_22 Interaction between RNU1 and SZR-22 723154 N/A 723157 unspecified role unspecified role prey prey N/A 723158 unspecified role unspecified role bait bait physical association physical association false false false rnu1-srz_21 Interaction between RNU1 and SZR-21 723154 N/A 723158 unspecified role unspecified role bait bait n/a 723159 unspecified role unspecified role prey prey physical association physical association false false false rnu1-srz_22-1 Interaction between RNU1 and SZR-22 723155 N/A 723158 unspecified role unspecified role bait bait N/A 723157 unspecified role unspecified role prey prey physical association physical association false false false Figure 1B rnu1-srz_21-1 Interaction between RNU1 and SZR-21 723155 N/A 723158 unspecified role unspecified role bait bait n/a 723159 unspecified role unspecified role prey prey physical association physical association false false false Figure 1B rnu1-srz_21-2 Interaction between RNU1 and SZR-21 723156 N/A 723158 unspecified role unspecified role bait bait region S-protein (104 aa, Ribonuclease A minus S-tag peptide sequence) tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) n/a 723159 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Figure 7C rnu1-srz_22-2 Interaction between RNU1 and SZR-22 723156 N/A 723157 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 723158 unspecified role unspecified role bait bait region S-protein (104 aa, Ribonuclease A minus S-tag peptide sequence) tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Figure 7C