IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ de_veylder-1997-3 The Arabidopsis Cks1At protein binds the cyclin-dependent kinases Cdc2aAt and Cdc2bAt. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot De Veylder L., Segers G., Glab N., Casteels P., Van Montagu M., Inze D. Purified Cks1At was coupled to CNBr-activated Sepharose 4B (Pharmacia, Uppsala, Sweden) at a concentration of 5 mg/ml of gel. Extracts from 2 day old Arabidopsis (ecotype Columbia) cell suspension cultures were used in this study. mamon@gengenp.rug.ac.be A cDNA fusion library of 3-week-old vegetative Arabidopsis plants FEBS Lett. (0014-5793) 1997 Only protein-protein interactions imex curation de_veylder-1997-2 The Arabidopsis Cks1At protein binds the cyclin-dependent kinases Cdc2aAt and Cdc2bAt. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined De Veylder L., Segers G., Glab N., Casteels P., Van Montagu M., Inze D. mamon@gengenp.rug.ac.be A cDNA fusion library of 3-week-old vegetative Arabidopsis plants FEBS Lett. (0014-5793) 1997 Only protein-protein interactions imex curation de_veylder-1997-1 The Arabidopsis Cks1At protein binds the cyclin-dependent kinases Cdc2aAt and Cdc2bAt. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence De Veylder L., Segers G., Glab N., Casteels P., Van Montagu M., Inze D. mamon@gengenp.rug.ac.be A cDNA fusion library of 3-week-old vegetative Arabidopsis plants FEBS Lett. (0014-5793) 1997 Only protein-protein interactions imex curation cks1_arath Cyclin-dependent kinases regulatory subunit 1 CKS1 At2g27960 T1E2.12 CKS1-At protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGQIQYSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLSENEWRAIGVQQSRGWVHYAVHRPEPHIMLFRRPLNYQQQQENQAQNMLVK cdka1_arath Cyclin-dependent kinase A-1 CDKA-1 Cell division control protein 2 homolog A CDC2 At3g48750 CDC2A T21J18.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDQYEKVEKIGEGTYGVVYKARDKVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHSNIVKLQDVVHSEKRLYLVFEYLDLDLKKHMDSTPDFSKDLHMIKTYLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSHHYSTPVDIWSVGCIFAEMISQKPLFPGDSEIDQLFKIFRIMGTPYEDTWRGVTSLPDYKSAFPKWKPTDLETFVPNLDPDGVDLLSKMLLMDPTKRINARAALEHEYFKDLGGMP ckb11_arath Cyclin-dependent kinase B1-1 CDKB1-1 At3g54180 F24B22.140 CDC2B Cell division control protein 2 homolog B protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEKYEKLEKVGEGTYGKVYKAMEKGTGKLVALKKTRLEMDEEGIPPTALREISLLQMLSTSIYVVRLLCVEHVHQPSTKSQSTKSNLYLVFEYLDTDLKKFIDSYRKGPNPKPLEPFLIQKLMFQLCKGVAHCHSHGVLHRDLKPQNLLLVKDKELLKIADLGLGRAFTVPLKSYTHEIVTLWYRAPEVLLGSTHYSTGVDMWSVGCIFAEMVRRQALFPGDSEFQQLLHIFRLLGTPTEQQWPGVSTLRDWHVYPKWEPQDLTLAVPSLSPQGVDLLTKMLKYNPAERISAKTALDHPYFDSLDKSQF cks1_schpo Cyclin-dependent kinases regulatory subunit suc1 SPBC1734.14c P13 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSKSGVPRLLTASERERLEPFIDQIHYSPRYADDEYEYRHVMLPKAMLKAIPTDYFNPETGTLRILQEEEWRGLGITQSLGWEMYEVHVPEPHILLFKREKDYQMKFSQQRGG cks1_human Cyclin-dependent kinases regulatory subunit 1 PNAS-16 PNAS-143 CKS1B CKS1 protein protein human Homo sapiens Human MSHKQIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTHLMSESEWRNLGVQQSQGWVHYMIHEPEPHILLFRRPLPKKPKK csk1-cdka1_1 Interaction between CSK1 and CDKA1-1 724965 N/A 724968 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 724969 unspecified role unspecified role prey prey arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. physical association physical association false false false Figure 6A The authors incorrectly state Figure 5A presents the in vitro interaction between CSK1 and CDKA1-1 result. csk1-cdkb1_1-1 Interaction between CSK1 and CDKB1-1 724965 N/A 724968 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 724970 unspecified role unspecified role prey prey arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. physical association physical association false false false Figure 6A The authors incorrectly state Figure 5A presents the in vitro interaction between CSK1 and CDKB1-1 result. suc1-cdka1_1-1 Interaction between S.Pombe Suc1 and CDKA1-1 724965 N/A 724971 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 724969 unspecified role unspecified role prey prey arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. physical association physical association false false false Figure 6C suc1-cdkb1_1-2 Interaction between S.Pombe Suc1 and CDKB1-1 724965 N/A 724970 unspecified role unspecified role prey prey arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. N/A 724971 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 6C The authors describe the interaction as 'weak' csk1-cdka1_1-1 Interaction between Human CSK1 and CDKA1-1 724965 N/A 724969 unspecified role unspecified role prey prey arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. N/A 724972 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 6C cdka1_1-csk1-1 Interaction between CDKA1-1 and CSK1 724966 N/A 724968 unspecified role unspecified role prey prey N/A 724969 unspecified role unspecified role bait bait physical association physical association false false false Figure 1 cdkb1_1-csk1-2 Interaction between CDKB1-1 and CSK1 724966 N/A 724970 unspecified role unspecified role bait bait N/A 724968 unspecified role unspecified role prey prey physical association physical association false false false Figure 1 cdka_1-csk1 Interaction between CDKA-1 and CSK1 724967 N/A 724969 unspecified role unspecified role bait bait N/A 724968 unspecified role unspecified role prey prey physical association physical association false false false