MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint nosaka-2008-1 Thiamin-dependent transactivation activity of PDC2 in Saccharomyces cerevisiae. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nosaka K., Onozuka M., Konno H., Akaji K. knosaka@koto.kpu-m.ac.jp FEBS Lett. (0014-5793) 2008 2008-12-02 Only protein-protein interactions imex curation nosaka-2008-2 Thiamin-dependent transactivation activity of PDC2 in Saccharomyces cerevisiae. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Nosaka K., Onozuka M., Konno H., Akaji K. knosaka@koto.kpu-m.ac.jp FEBS Lett. (0014-5793) 2008 2008-12-02 Only protein-protein interactions imex curation thi3_yeast Thiamine metabolism regulatory protein THI3 THI3 KID1 Keto isocaproate decarboxylase 1 Thiamine pyrophosphate-dependent 2-oxo-acid decarboxylase YDL080C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNSSYTQRYALPKCIAISDYLFHRLNQLNIHTIFGLSGEFSMPLLDKLYNIPNLRWAGNSNELNAAYAADGYSRLKGLGCLITTFGVGELSAINGVAGSYAEHVGILHIVGMPPTSAQTKQLLLHHTLGNGDFTVFHRIASDVACYTTLIIDSELCADEVDKCIKKAWIEQRPVYMGMPVNQVNLPIESARLNTPLDLQLHKNDPDVEKEVISRILSFIYKSQNPAIIVDACTSRQNLIEETKELCNRLKFPVFVTPMGKGTVNETDPQFGGVFTGSISAPEVREVVDFADFIIVIGCMLSEFSTSTFHFQYKTKNCALLYSTSVKLKNATYPDLSIKLLLQKILANLDESKLSYQPSEQPSMMVPRPYPAGNVLLRQEWVWNEISHWFQPGDIIITETGASAFGVNQTRFPVNTLGISQALWGSVGYTMGACLGAEFAVQEINKDKFPATKHRVILFMGDGAFQLTVQELSTIVKWGLTPYIFVMNNQGYSVDRFLHHRSDASYYDIQPWNYLGLLRVFGCTNYETKKIITVGEFRSMISDPNFATNDKIRMIEIMLPPRDVPQALLDRWVVEKEQSKQVQEENENSSAVNTPTPEFQPLLKKNQVGY 8EC37CC5B2BFA1AD thi2_yeast Thiamine biosynthesis regulatory protein THI2 PHO6 YBR240C YBR1624 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVNSKRQQRSKKVASSSKVPPTKGRTFTGCWACRFKKRRCDENRPICSLCAKHGDNCSYDIRLMWLEENIYKVRKHSLISSLQARKSKSKPLCQKISKSRFKQMTHFRQLSPPTSDCEDSVHEASKETTLPNDNTFTISVRRLKIYNNAVASVFGSMTNRDYTQKRIDKKLDELLNMVENDISVVNLNCSKHGPYSVFRANPAAVTSALTDQLPSPGHSMSSAEETTTAALSSPPEDSTSLIDIIQGKIFGILWFNCYGNMILNRQEYTTWFINKMRNSLTTEFIRFLGKIIDDPDINMASCLFKECIARWSCVDWQSIAITMLVIIHGYTCPNLTKLLRVWFLQQKLLRFSMYPLVNFIINNTQDLDVLYHCNGLLGNADLFEDPYQDELTSELHVLVTERLVNSWKDTILQQLCSCQDTTLSCSQLRYWQLQLKCNQQFYKDVYAMQD pdc2_yeast Protein PDC2 PDC2 D4451 YDR081C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSIQQRYNICLMAERHPKWTQLELAKWAYETFQLPKIPSQGTISRLLARKSTYMNCKEHEKDANRLRKPNNLLVRKILQEWISQSLWNGIPITSPIIQDTAQAVWHRIPAEHREGNGSFSYKWISNFLSKMDVNISVLDEELPKTPKVWTFEERDVLKAYFSKIPPKDLFTLDEAFLSYNLPLDYAQYEASSIQRRIEVATVMLCSNLDGSEKLKPVVVGKYDSYKSFRNYFPNEPNDPVSQSMLGTKMAKKFDISYHSNRKAWLTSNLFHNWLVRWDKRLVAVNRKIWIVLDDSCCHRIINLRLQNIKLVYTSSNSKFLPFNWGVWDEFKTRYRIQQYQALIDLQNRISKNIQNKNKSERNECIPNGKKCLISFEQSQLTMSNAFKFIKKAWDDIPVDAIKANWKSSGLLPPEMIHLNENVSMAFKKNEVLESVLNRLCDEYYCVKKWEYEMLLDLNIENKNTNFLSTEELVESAIVEPCEPDFDTAPKGNEVHDDNFDVSVFANEDDNNQNHLSMSQASHNPDYNSNHSNNAIENTNNRGSNNNNNNNGSSNNINDNDSSVKYLQQNTVDNSTKTGNPGQPNISSMESQRNSSTTDLVVDGNYDVNFNGLLNDPYNTMKQPGPLDYNVSTLIDKPNLFLSPDLDLSTVGVDMQLPSSEYFSEVFSSAIRNNEKAASDQNKSTDELPSSTAMANSNSITTALLESRNQAQPFDVPHMNGLLSDTSKSGHSVNSSNAISQNSLNNFQHNSASVAEASSPSITPSPVAINSTGAPARSIISAPIDSNSSASSPSALEHLEGAVSGMSPSSTTILSNLQTNINIAKSLSTIMKHAESNEISLTKETINELNFNYLTLLKRIKKTRKQLNSESIKINSKNAQDHLETLLSGAAAAAATSANNLDLPTGGSNLPDSNNLHLPGNTGFF thi3-thi2 773865 thi3_yeast THI3 773867 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false thi2_yeast THI2 773868 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false F2 mint thi3-pdc2 773865 thi3_yeast THI3 773867 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q07471:p.Asn488Asp mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false pdc2_yeast PDC2 773869 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false F2 mint thi3-pdc2-1 773865 pdc2_yeast PDC2 773869 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false thi3_yeast THI3 773867 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false F4 mint thi3-pdc2-2 773866 thi3_yeast THI3 773867 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified Q07471:p.Asn488Asp mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdc2_yeast PDC2 773869 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false F3 mint