IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lin-2008a-3 Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down anti tag western anti tag western blot anti tag western Lin CH., Macgurn JA., Chu T., Stefan CJ., Emr SD. sde26@cornell.edu Cell (0092-8674) 2008 Only protein-protein interactions 10 imex curation lin-2008a-1 Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lin CH., Macgurn JA., Chu T., Stefan CJ., Emr SD. sde26@cornell.edu Cell (0092-8674) 2008 Only protein-protein interactions 10 imex curation lin-2008a-2 Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Lin CH., Macgurn JA., Chu T., Stefan CJ., Emr SD. Yeast cells were stimulated with methionine, treated with crosslinkers (DSP and DTBP), and TCA precipitated. FLAG immunoprecipitates were analyzed by SDS PAGE and immunoblot. sde26@cornell.edu Cell (0092-8674) 2008 Only protein-protein interactions 10 imex curation ldb19_yeast Protein LDB19 LDB19 Low dye-binding protein 19 O6152 YOR322C protein protein yeast Saccharomyces cerevisiae Baker's yeast MAFSRLTSTHQSNHNGYSNSNKKGQSLPLTLSIDVESPPCVLYGSAMESSGAVLSGLFTVTVVDPYSSAEDKSLKNTESNVSTTSKSLKRKSTFGSALSSRLSSLSASTSNISPSTSSTSISHSPTPANLRIMAGYTKITITSVTLSLVQKIHFHKPFVPNISSMQTCMNCKTKITNMKSWEIQSNTQDLSVGSHSYPFSYLIPGSVPCSSSLGATAETQVKYELIAVVTYIDPHRNSFSSGHSTPRKEGSSSKKRLLQLAMPIAVTRSIPRGPDKNSLRVFPPTELTAAAVLPNVVYPKSTFPLEMKLDGVSSGDRRWRMRKLSWRIEETTRVKAHACPVHKHELRQLEEQVKIKESEKSKKPRSHIKRYGELGPQIRVAVNSLENMPSQRLPGEPGREQAPNSSGPASTGNVGLDDENPVNEDEEDQPGSEFIHPSDDALRQELLMQQQRARQQQLQQELKNNSSLFTEEVRIISKGEMKSGWKTDFDNNGKIELVTEIDCMGLNSGVSNPVMHASTLQTPSTGNKKPSINVACDIQDPNLGLYVSHILAVEIVVAEETLQYANGQPIRKPNSKNKKETNNNTMNVHNPDQRLAELSPIFANRNTPKVRRMGPEDITPVNSNKSNHSTNKEKASNGASNSNIVSVPTGAARVLRMQFRLTVTERSGLGISWDEEVPPIYQDVELLSPPCYELSINNGIKNKLYSTMSTPVRSEDDFVGGSDEDIGNYESQGLEPGPNVQEVTITQNKLTIPPTAHHYQPASSSQRSLTTVQSPPLESVVSVQGSVPFRGHVLTPHSTRDIRIQNFSDFLDSNRITQ rsp5_yeast E3 ubiquitin-protein ligase RSP5 RSP5 MDP1 NPI1 YER125W SYGP-ORF41 Reverses SPT-phenotype protein 5 HECT-type E3 ubiquitin transferase RSP5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPSSISVKLVAAESLYKRDVFRSPDPFAVLTIDGYQTKSTSAAKKTLNPYWNETFKFDDINENSILTIQVFDQKKFKKKDQGFLGVVNVRVGDVLGHLDEDTATSSGRPREETITRDLKKSNDGMAVSGRLIVVLSKLPSSSPHSQAPSGHTASSSTNTSSTTRTNGHSTSSTRNHSTSHPSRGTAQAVESTLQSGTTAATNTATTSHRSTNSTSSATRQYSSFEDQYGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDQTEAERGNQLNANTELERRQHRGRTLPGGSSDNSSVTVQVGGGSNIPPVNGAAAAAFAATGGTTSGLGELPSGWEQRFTPEGRAYFVDHNTRTTTWVDPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLENSIDGVLDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWKCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEVQQLPKSHTCFNRVDLPQYVDYDSMKQKLTLAVEETIGFGQE 6F1836384479E70F ecm21_yeast Protein ECM21 YBL101C YBL0814 ECM21 Extracellular mutant protein 21 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPFITSRPVAKNSSHSLSETDLNQSKGQPFQPSPTKKLGSMQQRRRSSTIRHALSSLLGGANVHSPAVLNNTTKGGNNNGNIRSSNTDAQLLGKKQNKQPPPNARRHSTTAIQGSISDSATTTPRSSTSDTNRRTSGRLSVDQEPRISGGRYSQIEEDSTVLDFDDDHNSSAVVSSDLSSTSLTRLANSKKFNEQFLIEYLTARGLLGPKTVLSNEYLKISISTSGESVFLPTISSNDDEYLSRLNGLNDGTDDAEADFFMDGIDQQEGNTPSLATTAAATESGGSINENRDTLLRENNSGDHPGSGSELNTRSVEIDSSMVSYSIAVIVSVKKPTRFTDMQLELCSRVKVFWNTGVPPTKTFNEEFYNAASMKWNLNDENFDLFVPLSISPDDQMIENNSNDRQMRLFKNIPTEERLYLDKTKTKASLLNAIDVNKTHLYQPGDYVFLVPVVFSNHIPETIYLPSARVSYRLRLATKAINRKGFYRQDSNSPQPIVSPDSSSSLSSTTSSLKLTETESAQAHRRISNTLFSKVKNHLHMSSHQLKNEESGEEDIFAEYPIKVIRTPPPVAVSTANKPIYINRVWTDSLSYEISFAQKYVSLNSEVPIKIKLAPICKNVCVKRIHVSITERVTFVSKGYEYEYDQTDPVAKDPYNPYYLDFASKRRKERSVSLFEIRTKEKGTRALREEIVENSFNDNLLSYSPFDDDSDSKGNPKERLGITEPIIIETKLKFPKYEDLDKRTAKIIPPYGIDAYTSIPNPEHAVANGPSHRRPSVIGFLSGHKGSKSHEENEKPVYDPKFHQTIIKSNSGLPVKTHTRLNTPKRGLYLDSLHFSNVYCRHKLEIMLRISKPDPECPSKLRHYEVLIDTPIFLVSEQCNSGNMELPTYDMATMEGKGNQVPLSMNSDFFGNTCPPPPTFEEAISVPASPIVSPMGSPNIMASYDPDLLSIQQLNLSRTTSVSGPSGYSDDAGVPNVNRNSISNANAMNGSISNSAFVSGNSGQGVARARATSVNDRSRFNNLDKLLSTPSPVNRSHNSSPTNGLSQANGTVRIPNATTENSKDKQNEFFKKGYTLANVKDDEEQEGIVSSSSADSLLSHGNEPPRYDEIVPLMSDEE aly2_yeast Arrestin-related trafficking adapter 3 ART3 J0934 YJL084C ALY2 Arrestin-like protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPMDQSISSPLFPMEKDIDIPLDATPLAQSSSLQLFIHLAEPVVFLQGFDPQKTEYPSVVLRGCLVVRILKPTKLKSISLSFKGYSRTEWPEGIPPKRQEFVEIKDIVDHTWALYPPTEQKSKKKMDASAPNESNNAANNFLTKESGASLYRTLSDNETITSRKNSISGLSSLNLSPLGAPGNSSVNVKDRESRQRSRSSSVTSSNGPSRNLSPINLLKRATSPSVSHHNYKPTTTSIFSDLLNNTFTHNDAASHHGHHIPTSSNHLAMTSNNFTSGSGGEFFVFQPGDYIYAFEELIPQAYPESIKADFGFVEYFLFASIERPGAFKSNISARQVVNIVRTQAHNSVEESEPIIISRDWENQLYYDIVIASKDIILDAFLPITFKFAPLDKVTLHRIRIYVTETMEYYCREKKVHRMEPTKKFLLTEQKGPKLPNLPNDANLSKAKNMGNLLQDPKNGDLVNKEYEYQIFIPSRFNNHQQLHPDTSYENIKANHWIKICLRLSRVVDNKRKHYEISIDSPIHVLHRLCSHANTLLPSYDGHPASFPKETDSSISSILESSDDNINLYHNSNIFFPKEVLSSPVLSPNVQPLDILIPHLPSTSLTRNSRQFNRNSKSHPSDNTIFNSAKLKSNIYQPESLQRELASPQAIPLSPITSPMSNMEVPPPDFDFSSDFISDAASGTTTTEVSSSESSILPRDPPSYKDTVLHDNNQKRRPNSKHPTPPSLKASHPNKNSDKNSSETLNKKESMSKIEENKHKRETTPKKRENRDVKSLSTPQREESKDSTSTGNQSNEKNRKRVLSLSSSLHSSPNNSGFAHSALGNLSNESLRSLNRRESVQDNLPSTIRHDNPFFTDLNQVLIEDELKNHDKNELNRHSTNTSSTPASARSSFDYSGINISKDKLNMEPLLSKTETLTNKVNEDSFLRPNDSYVDLLEPSVDTTIDITAPYARNSSAWHPLQNDNDNNQFSPLLGSNENFLNAANAQNSAESDHNNDIFTQGSGLTESSKNSDSEERFISRLSSPEKVLINTLDNESGLQSINESTL rod1_yeast Protein ROD1 ROD1 YOR018W OR26.08 Resistance to o-dinitrobenzene protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSSSSRPSKEPLLFDIRLRNLDNDVLLIKGPPDEASSVLLSGTIVLSITEPIQIKSLALRLFGRLRLNIPTVLQTVHGPHKRYSKFERNIYSHFWDDFNIKSYFQNLYDNHNNGKITISSKSSTNLAALPKRKRALSTASLISSNGQTSASKNYHTLVKGNYEFPFSAIIPGSLVESVEGLPNAAVTYALEATIERPKQPDLICKKHLRVIRTLAIDAVELSETVSVDNSWPEKVDYTISIPTKAIAIGSSTMINILIVPILKGLKLGPVRISLVENSQYCGSYGGVINQERMVAKLKLKDPLKHVAQIKKKRSLNEAADEGVDTDTGEFQDKWEVRALLNIPASLTKCSQDCRILSNIKVRHKIKFTISLLNPDGHISELRAALPVQLFISPFVPVNVKTSDVIERTLKTFGPSYQVTSQHDNSFSSKNFVDDSEEDVIFQRSASALQLSSMPTIVSGSTLNINSTDAEATAVADTTMVTSLMVPPNYGNHVYDRVYGEVTNEDETSASASSSAVESQAIHNIQNLYISDSNNSNNPILAPNPQIKIEDDSLNNCDSRGDSVNNSNLNLVNSNLTISENWNNNSPSANRYNNIINAGLNSPSLTPSFAHLSRRNSYSRQTSSTSLKNDLELTDLSRVPSYDKAMKSDMIGEDLPPAYPEEELGVQENKKIELERPQILHHKSTSSLLPLPGSSKSSNNLKRSSSRTHLSHSPLPRNNSGSSVSLQQLARNNTDSSFNLNLSFTSAKSSTGSRHFPFNMTTSFTSNSSSKNNSHFDKTDSTSDANKPREEENYTSATHNRRSRSSSVRSNNSNSPLRQGTGSFANLMEMFTKRDRS FD9A64174B1DC443 art5_yeast Arrestin-related trafficking adapter 5 YGR068C ART5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSLSSLSSSGGHSEQKERERISYFDIRINSPYKDIILIQGSPLELSSIPLSGNLVISVKNEIVVKKISLRLVGRFKLEFLQVGRYKKNSSSLASLVKEKRKIFECYWDNLLVSSKGDVLVGGENAENQHNSSSGRSTSNQDMDTSGNAIFLSKRSLSSPVFNKIIRRKTHSSHRKILELPENGVTGTPFEGLRENARSRSSSSNTLNNNSHSYSNRDGSGSSYLFLMKRGNYELPFNTMLPPEVCETIEGLQSGSILYSFEAIIDGRQLWDTDLSVHTSPHGPIGSTSTSGNGMRTKNKIIIKKFKYLRILRTLSMDNLAMQEEISVGNTWRDKLQYETSIPSRAVPIGSTTPVKIKIFPFEKNIRLDRIEMALIQYYAMKDSSAQIYDDEIAVMKITHLADFGPLTDKLDVDCPFTIPDNLKQITQDCCLQDNLIRVMHKLQVRILLQRQVDGEYKNLEIKAQLPMLLFISPHLPMKGRLVLFDKHDGKIHFRPGELVPLFLTTYPAQGLTPGVELNSTTTAHLALPQPPPNYHESTNDHLMPALQPLGADSVVLTVPSYEQAQAQASASSYVTGSVPAYCDDD aly1_yeast Arrestin-related trafficking adapter 6 ART6 YKR021W ALY1 Arrestin-like protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLQFNTENDTVAPVFPMEQDINAAPDAVPLVQTTTLQVFVKLAEPIVFLKGFETNGLSEIAPSILRGSLIVRVLKPNKLKSISITFKGISRTEWPEGIPPKREEFSDVETVVNHTWPFYQADDGMNSFTLEHHSSNNSSNRPSMSDEDYLLEKSGASVYIPPTAEPPKDNSNLSLDAYERNSLSSDNLSNKPVSSDVSHDDSKLLAIQKTPLPSSSRRGSVPANFHGNSLSPHTFISDLFTKTFSNSGATPSPEQEDNYLTPSKDSKEVFIFRPGDYIYTFEQPISQSYPESIKANFGSVEYKLSIDIERFGAFKSTIHTQLPIKVVRLPSDGSVEETEAIAISKDWKDLLHYDVVIFSKEIVLNAFLPIDFHFAPLDKVTLHRIRIYLTESMEYTCNSNGNHEKARRLEPTKKFLLAEHNGPKLPHIPAGSNPLKAKNRGNILLDEKSGDLVNKDFQFEVFVPSKFTNSIRLHPDTNYDKIKAHHWIKICLRLSKKYGDNRKHFEISIDSPIHILNQLCSHANTLLPSYESHFQYCDEDGNFAPAADQQNYASHHDSNIFFPKEVLSSPVLSPNVQKMNIRIPSDLPVVRNRAESVKKSKSDNTSKKNDQSSNVFASKQLVANIYKPNQIPRELTSPQALPLSPITSPILNYQPLSNSPPPDFDFDLAKRGAADSHAIPVDPPSYFDVLKADGIELPYYDTSSSKIPELKLNKSRETLASIEEDSFNGWSQIDDLSDEDDNDGDIASGFNFKLSTSAPSENVNSHTPILQSLNMSLDGRKKNRASLHATSVLPSTIRQNNQHFNDINQMLGSSDEDAFPKSQSLNFNKKLPILKINDNVIQSNSNSNNRVDNPEDTVDSSVDITAFYDPRMSSDSKFDWEVSKNHVDPAAYSVNVASENRVLDDFKKAFREKRK rog3_yeast Protein ROG3 YFR022W ROG3 Revertant of glycogen synthase kinase mutation protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGFSSGKSTKKKPLLFDIRLKNVDNDVILLKGPPNEAPSVLLSGCIVLSINEPMQIKSISLRLYGKIQIDVPLERPQDASSSSLSSSPPKIRKYNKVFYNYAWDNVNLKEYLSGLRGQSGLAGSSSSSNILGTRQRAQSTSSLKSLKGSSSPSSCTLDKGNYDFPFSAILPGSLPESVESLPNCFVTYSMESVIERSKNYSDLICRKNIRVLRTISPAAVELSETVCVDNSWPDKVDYSISVPNKAVAIGSATPINISIVPLSKGLKLGSIKVVLFENYQYCDPFPPVISENRQVTELNLEDPLNESSGEFNGNGCFVNNPFFQPDHSFQDKWEIDTILQIPNSLSNCVQDCDVRSNIKVRHKLKFFIILINPDGHKSELRASLPIQLFISPFVALSIKPLSSSNLYSLFSTTNQKDENSSQEEEEEYLFSRSASVTGLELLADMRSGGSVPTISDLMTPPNYEMHVYDRLYSGSFTRTAVETSGTCTPLGSECSTVEDQQQDLEDLRIRLTKIRNQRDNLGLPPSASSAAASRSLSPLLNVPAPEDGTERILPQSALGPNSGSVPGVHSNVSPVLLSRSPAPSVSAHEVLPVPSGLNYPETQNLNKVPSYGKAMKYDIIGEDLPPSYPCAIQNVQPRKPSRVHSRNSSTTLSSSIPTSFHSSSFMSSTASPISIINGSRSSSSGVSLNTLNELTSKTSNNPSSNSMKRSPTRRRATSLAGFMGGFLSKGNKR csr2_yeast Transcription factor CSR2 CHS5 SPA2 rescue protein 2 CSR2 MRG19 YP9367.10 YPR030W protein protein yeast Saccharomyces cerevisiae Baker's yeast MQSTVPIAIASNGNKRDVVQNVSAGDEGDILQRLARNREMISTSLSPQKSSGFSGRRRSSSVRDALSSFFGTGNSPTSSMDDYSNLMNRNYSTASTAMCRGNSFPSDVGTKAYNITGSYQPDRHRNSVPYTTIDQLHTRQDTGLRRESDPVAAKQISSNNDIVRSFITHHASNSTMFINRVLSDYLADRGFIKQTPLYNKKSVLEISIATSAESVFLPTTKSDETEYLSLIHGSLNQARTQPVGSTNTAESDFLPSCPTMDTLNENNDLSLFPLHTQRTSPSNTARTGNAMDTSNSDRASPASNNNTTDADSFVASGNNNPMNNNNSPARNRHPNSHSRSLPNAWNSQMPSFSFALIFSLNKSTTLSDIKVELTSNVRVVWFNGLPPTKNVNEECYNIGSLDWTLNADNFNLFIPQGAKSPLDIVENHSNNRKLKVLQKLSMRKRRSFSNKAVLRENILNNLNASNSTNKLNAGVYVFTIPIVLASRIPESLYYPSARVSYSLRLATKLKDEHTQLVASRPRSSSISSPQKLRSYSCSDSYEYSQIDDTIEGETYNNDKNSTGKIAFPSSWLKSAKGRLKRNNSNGRSDNNGASSSGLAMQHDSEDTINLQYPLNLVRTPPEISVTTANKPLYINKVWENCLSYEISFAQKYVPLNGEIPITIKVAPLVKSLSVKRIRVSCREKISYRSKDYQYDFDQLDPLASDPCNPYHMRYLVRKKKDRSLPLFEVASKCTSGPSIREEVVTNTVDDNLLAYTSSKENNKDIPFSESFTVKTKLKFPKYCEVDATKAASLPPYGIDLFDPIKDPTQSENTSNNGNVLGFLVGRPNRASKTVHKIPQDKNHNEVNDTNGNSNTSLQTSSNVPIQHYTRLNKPRRGLYLDSMHFKNIQCSHKLEIVLRVSKTDSGSSKIIRHYEVIVDTPIYLISDLCNTSNIDLPTYDMATTESSKVLPPTFEEATSVSASPRSSVSYYPDDISMQQLNLSRSTSLANGYLSTLHPKTTAVSDSSNGAPIRDQQEQQARPLRTEDYALQMGNENNAYSNMDGLLSQDIFEQETAATLFKRDIVTMNFNNNIFTPRYSPRTFTNTDYNYNDNDNNDNDTEGPGPIIHPGPEPPRYDEISS 31F958BB8DB0B7E8 mup1_yeast High-affinity methionine permease MUP1 YGR055W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEGRTFLSQLNVFNKENYQFSSSTTKKEVSNSTVDADNGASDFEAGQQFATELDQGEKQLGILSCIGLICNRMLGTGVFAVSSTIYTLCGSVGLALIMWAVGAIIAISGLYVYMEFGTAIPKNGGEKNYLEAIFRKPKFFITCMYAAYIFFLGWAAGNSINTAIMFLTAADTEVTKWNQRGIGVAVVFFAFLINSLNVKIGLYLQNILGIFKIGIVLFISITGWVALGGGLKDGYQSHNFRNAFEGTETATAYGIVNALYSVIWSFVGYSNVNYALGEVKNPVRTLKIAGPTSMVFLAIIYIFVNIAYFAVVPKDKLISSKLILAADFFDIVFGGQAKRAAAALVGLSALGNVLSVIFSQGRIIQQLGREGVLPFSNFFASSKPFNSPMVGLFQHFIVCTVTILAPPPGDAYLLVQNLISYPMNIINFAISAGLLWIYWQRRQGKIEWNPPIKAGVFVTGFFTLSNLYLIIAPYVPPSNGESVYSSMPYWIHCVIAWGIFFFGGVYYVVWAQLLPRWGHYKLVSKDVLGEDGFWRVKIAKVYDDTIGDVDTQEDGVIETNIIEHYKSEQEKSL rsp5-ldb19-2 Interaction between rsp5 and ldb19 identified by pulldown 778444 n/a 778447 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 778448 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false ecoli Escherichia coli (strain K12) 778463 778459 physical association physical association false false false Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-ecm21 Interaction between rsp5 and ecm21(art2) identified by pulldown 778444 n/a 778448 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false ecoli Escherichia coli (strain K12) n/a 778449 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry A c-terminal fragment of ecm21 was required to bind but the coordinates were not given in the entry rsp5-yjl084c-1 Interaction between rsp5 and YJL084C identified by pulldown 778444 n/a 778450 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 778448 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-rod1-1 Interaction between rsp5 and rod1 (art4) identified by pulldown 778444 n/a 778451 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 778448 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-ygr068c Interaction between rsp5 and ygr068c (art5) identified by pulldown 778444 n/a 778452 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 778448 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-ygr068c-1 Interaction between rsp5 and ykr021w (art6) identified by pulldown 778444 n/a 778453 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 778448 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-rog3-1 Interaction between rsp5 and rog3 (art7) identified by pulldown 778444 n/a 778448 unspecified role unspecified role bait bait ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 778454 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-rog3-2 Interaction between rsp5 and csr2 (art8) identified by pulldown 778444 n/a 778448 unspecified role unspecified role bait bait ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 778455 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-ldb19-1 Interaction between rsp5 and ldb19 identified by pulldown 778445 n/a 778447 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q12502:p.[Pro679Ala;Tyr681Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q12502:p.[Pro690Ala;Tyr692Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 778448 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 2E Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry mup1-swi4-1 Crosslinking of mup1 and ldb19 (art1) 778446 n/a 778447 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 778456 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4E