IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ datta-2008-1 LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-Box Protein Involved in Light-Dependent Development and Gene Expression, Undergoes COP1-Mediated Ubiquitination. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Datta S., Johansson H., Hettiarachchi C., Irigoyen ML., Desai M., Rubio V., Holm M. magnus.holm@gu.se Plant Cell (1040-4651) 2008 Only protein-protein interactions imex curation datta-2008-2 LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-Box Protein Involved in Light-Dependent Development and Gene Expression, Undergoes COP1-Mediated Ubiquitination. allce Allium cepa (Onion) Onion fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Datta S., Johansson H., Hettiarachchi C., Irigoyen ML., Desai M., Rubio V., Holm M. The tagged proteins were transformed into onion tissue by partical bombardment. magnus.holm@gu.se Plant Cell (1040-4651) 2008 Only protein-protein interactions imex curation datta-2008-3 LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-Box Protein Involved in Light-Dependent Development and Gene Expression, Undergoes COP1-Mediated Ubiquitination. allce Allium cepa (Onion) Onion confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Datta S., Johansson H., Hettiarachchi C., Irigoyen ML., Desai M., Rubio V., Holm M. The tagged proteins were transformed into onion tissue by partical bombardment. magnus.holm@gu.se Plant Cell (1040-4651) 2008 Only protein-protein interactions imex curation datta-2008-4 LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-Box Protein Involved in Light-Dependent Development and Gene Expression, Undergoes COP1-Mediated Ubiquitination. in vitro In vitro enzymatic study enzymatic study anti tag western anti tag western blot anti tag western Datta S., Johansson H., Hettiarachchi C., Irigoyen ML., Desai M., Rubio V., Holm M. magnus.holm@gu.se Plant Cell (1040-4651) 2008 Only protein-protein interactions imex curation hy5_arath Transcription factor HY5 HY5 BZIP56 At5g11260 F2I11_150 Protein LONG HYPOCOTYL 5 bZIP transcription factor 56 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQEQATSSLAASSLPSSSERSSSSAPHLEIKEGIESDEEIRRVPEFGGEAVGKETSGRESGSATGQERTQATVGESQRKRGRTPAEKENKRLKRLLRNRVSAQQARERKKAYLSELENRVKDLENKNSELEERLSTLQNENQMLRHILKNTTGNKRGGGGGSNADASL bbx22_arath B-box zinc finger protein 22 BBX22 DBB3 LZF1 Protein DOUBLE B-BOX 3 Protein LIGHT-REGULATED ZINC FINGER PROTEIN 1 Protein SALT TOLERANCE HOMOLOG 3 STH3 T30F21.7 At1g78600 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKIQCNVCEAAEATVLCCADEAALCWACDEKIHAANKLAGKHQRVPLSASASSIPKCDICQEASGFFFCLQDRALLCRKCDVAIHTVNPHVSAHQRFLLTGIKVGLESIDTGPSTKSSPTNDDKTMETKPFVQSIPEPQKMAFDHHHHQQQQEQQEGVIPGTKVNDQTSTKLPLVSSGSTTGSIPQWQIEEIFGLTDFDQSYEYMENNGSSKADTSRRGDSDSSSMMRSAEEDGEDNNNCLGGETSWAVPQIQSPPTASGLNWPKHFHHHSVFVPDITSSTPYTGSSPNQRVGKRRRRF hyh_arath Transcription factor HY5-like HY5 homolog HYH BZIP64 bZIP transcription factor 64 MKP6.27 At3g17609 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSLQRPNGNSSSSSSHKKHKTEESDEELLMVPDMEAAGSTCVLSSSADDGVNNPELDQTQNGVSTAKRRRGRNPVDKEYRSLKRLLRNRVSAQQARERKKVYVSDLESRANELQNNNDQLEEKISTLTNENTMLRKMLINTRPKTDDNH cop1_arath E3 ubiquitin-protein ligase COP1 COP1 Constitutive photomorphogenesis protein 1 RING-type E3 ubiquitin transferase COP1 T21L14.11 At2g32950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEISTDPVVPAVKPDPRTSSVGEGANRHENDDGGSGGSEIGAPDLDKDLLCPICMQIIKDAFLTACGHSFCYMCIITHLRNKSDCPCCSQHLTNNQLYPNFLLDKLLKKTSARHVSKTASPLDQFREALQRGCDVSIKEVDNLLTLLAERKRKMEQEEAERNMQILLDFLHCLRKQKVDELNEVQTDLQYIKEDINAVERHRIDLYRARDRYSVKLRMLGDDPSTRNAWPHEKNQIGFNSNSLSIRGGNFVGNYQNKKVEGKAQGSSHGLPKKDALSGSDSQSLNQSTVSMARKKRIHAQFNDLQECYLQKRRQLADQPNSKQENDKSVVRREGYSNGLADFQSVLTTFTRYSRLRVIAEIRHGDIFHSANIVSSIEFDRDDELFATAGVSRCIKVFDFSSVVNEPADMQCPIVEMSTRSKLSCLSWNKHEKNHIASSDYEGIVTVWDVTTRQSLMEYEEHEKRAWSVDFSRTEPSMLVSGSDDCKVKVWCTRQEASVINIDMKANICCVKYNPGSSNYIAVGSADHHIHYYDLRNISQPLHVFSGHKKAVSYVKFLSNNELASASTDSTLRLWDVKDNLPVRTFRGHTNEKNFVGLTVNSEYLACGSETNEVYVYHKEITRPVTSHRFGSPDMDDAEEEAGSYFISAVCWKSDSPTMLTANSQGTIKVLVLAA q8sbc1_orysj OsRad6 OSNPB_030791800 LOC_Os03g57790 Os03g0791800 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MSTPARKRLMRDFKRLQQDPPAGISGAPHDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPVVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRKVREIVEQSWTAD uba3_yeast NEDD8-activating enzyme E1 catalytic subunit Ubiquitin-activating enzyme E1 3 RUB1-activating enzyme E1 Ubiquitin-like protein-activating enzyme UBA3 YPR066W YP9499.21 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDCKILVLGAGGLGCEILKNLTMLSFVKQVHIVDIDTIELTNLNRQFLFCDKDIGKPKAQVAAQYVNTRFPQLEVVAHVQDLTTLPPSFYKDFQFIISGLDAIEPRRFINETLVKLTLESNYEICIPFIDGGTEGLKGHVKTIIPGITACWECSIDTLPSQQDTVPMCTIANNPRCIEHVVEYVSTIQYPDLNIESTADMEFLLEKCCERAAQFSISTEKLSTSFILGIIKSIIPSVSTTNAMVAATCCTQMVKIYNDLIDLENGNNFTLINCSEGCFMYSFKFERLPDCTVCSNSNSN at1g78600-hy5 At1g78600 (STH3) interacts with HY5 767823 n/a 767827 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 767828 unspecified role unspecified role prey prey physical association physical association false false false Figure 1 at1g78600-hyh At1g78600 (STH3) interacts with HYH 767823 n/a 767828 unspecified role unspecified role prey prey n/a 767829 unspecified role unspecified role bait bait physical association physical association false false false Figure 1 at1g78600-hy5-3 At1g78600 (STH3) interacts with HY5 767823 n/a 767828 unspecified role unspecified role prey prey Q9SYM2:p.Asp72Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q9SYM2:p.Asp81Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false n/a 767827 unspecified role unspecified role bait bait physical association physical association false false false Figure 1 at1g78600-hy5-2 At1g78600 (STH3) interacts with HY5 767824 n/a 767828 unspecified role unspecified role fluorescence donor fluorescence donor region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 767827 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 2 at1g78600-cop1 At1g78600 (STH3) interacts with COP1 767825 n/a 767830 unspecified role unspecified role neutral component neutral component n/a 767828 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Figure 2 cop1-at1g78600 COP1 ubiquitinates At1g78600 767826 n/a 767831 unspecified role unspecified role ancillary ancillary ecoli Escherichia coli (strain K12) n/a 767832 unspecified role unspecified role ancillary ancillary ecoli Escherichia coli (strain K12) n/a 767828 enzyme target enzyme target substrate substrate neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 767830 enzyme enzyme neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) ubiquitination ubiquitination reaction ubiquitination false false false Figure 6 Ubiquitination of Q9SYM2