UniProt UniProt http://www.uniprot.org madsen-2008-5 Ubxd1 is a novel co-factor of the human p97 ATPase. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk HeLa cDNA Int. J. Biochem. Cell Biol. (1357-2725) 2008 Only protein-protein interactions imex curation madsen-2008-1 Ubxd1 is a novel co-factor of the human p97 ATPase. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk HeLa cDNA Int. J. Biochem. Cell Biol. (1357-2725) 2008 Only protein-protein interactions imex curation madsen-2008-2 Ubxd1 is a novel co-factor of the human p97 ATPase. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk HeLa cDNA Int. J. Biochem. Cell Biol. (1357-2725) 2008 Only protein-protein interactions imex curation madsen-2008-3 Ubxd1 is a novel co-factor of the human p97 ATPase. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk HeLa cDNA Int. J. Biochem. Cell Biol. (1357-2725) 2008 Only protein-protein interactions imex curation madsen-2008-4 Ubxd1 is a novel co-factor of the human p97 ATPase. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk HeLa cDNA Int. J. Biochem. Cell Biol. (1357-2725) 2008 Only protein-protein interactions imex curation tera_human Transitional endoplasmic reticulum ATPase VCP 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein human Homo sapiens Human MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG syvn1_human E3 ubiquitin-protein ligase synoviolin Synovial apoptosis inhibitor 1 SYVN1 HRD1 KIAA1810 RING-type E3 ubiquitin transferase synoviolin protein protein human Homo sapiens Human MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKVMGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLAEDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSILTRGASVQLVFGFEYAILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHTFPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMVTGAKRLPCNHIFHTSCLRSWFQRQQTCPTCRMDVLRASLPAQSPPPPEPADQGPPPAPHPPPLLPQPPNFPQGLLPPFPPGMFPLWPPMGPFPPVPPPPSSGEAVAPPSTSAAALSRPSGAATTTAAGTSATAASATASGPGSGSAPEAGPAPGFPFPPPWMGMPLPPPFAFPPMPVPPAGFAGLTPEELRALEGHERQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNSTEETATTVVAAASSTSIPSSEATTPTPGASPPAPEMERPPAPESVGTEEMPEDGEPDAAELRRRRLQKLESPVAH ubxn6_human UBX domain-containing protein 6 UBXN6 UBXD1 UBXDC2 UBX domain-containing protein 1 protein protein human Homo sapiens Human MKKFFQEFKADIKFKSAGPGQKLKESVGEKAHKEKPNQPAPRPPRQGPTNEAQMAAAAALARLEQKQSRAWGPTSQDTIRNQVRKELQAEATVSGSPEAPGTNVVSEPREEGSAHLAVPGVYFTCPLTGATLRKDQRDACIKEAILLHFSTDPVAASIMKIYTFNKDQDRVKLGVDTIAKYLDNIHLHPEEEKYRKIKLQNKVFQERINCLEGTHEFFEAIGFQKVLLPAQDQEDPEEFYVLSETTLAQPQSLERHKEQLLAAEPVRAKLDRQRRVFQPSPLASQFELPGDFFNLTAEEIKREQRLRSEAVERLSVLRTKAMREKEEQRGLRKYNYTLLRVRLPDGCLLQGTFYARERLGAVYGFVREALQSDWLPFELLASGGQKLSEDENLALNECGLVPSALLTFSWDMAVLEDIKAAGAEPDSILKPELLSAIEKLL herp1_human Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein Methyl methanesulfonate (MMF)-inducible fragment protein 1 HERPUD1 KIAA0025 MIF1 HERP protein protein human Homo sapiens Human MESETEPEPVTLLVKSPNQRHRDLELSGDRGWSVGHLKAHLSRVYPERPRPEDQRLIYSGKLLLDHQCLRDLLPKQEKRHVLHLVCNVKSPSKMPEINAKVAESTEEPAGSNRGQYPEDSSSDGLRQREVLRNLSSPGWENISRPEAAQQAFQGLGPGFSGYTPYGWLQLSWFQQIYARQYYMQYLAATAASGAFVPPPSAQEIPVVSAPAPAPIHNQFPAENQPANQNAAPQVVVNPGANQNLRMNAQGGPIVEEDDEINRDWLDWTYSAATFSVFLSILYFYSSLSRFLMVMGATVVMYLHHVGWFPFRPRPVQNFPNDGPPPDVVNQDPNNNLQEGTDPETEDPNHLPPDRDVLDGEQTSPSFMSTAWLVFKTFFASLLPEGPPAIAN nsf1c_rat NSFL1 cofactor p47 Nsfl1c p97 cofactor p47 XY body-associated protein XY40 protein protein rat Rattus norvegicus (Rat) Rat MAEERQDALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEEEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGERRRHSGQDVHVVLKLWKTGFSLDNGDLRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLNTSSPAQQAENEAKASSSILINEAEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFVLMTTFPNKELADENQTLKEANLLNAVIVQRLT ubxn6-syvn1 777033 n/a 777038 unspecified role unspecified role prey prey n/a 777039 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 777040 unspecified role unspecified role bait bait physical association physical association false false false 3c Q86TM6 transfection ubxn6-herpud1 777033 n/a 777041 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 777040 unspecified role unspecified role bait bait physical association physical association false false false 3c Q15011 transfection vcp-ubxn6 777034 n/a 777040 unspecified role unspecified role prey prey n/a 777038 unspecified role unspecified role bait bait physical association physical association false false false ubxn6-vcp 777035 n/a 777038 unspecified role unspecified role prey prey n/a 777040 unspecified role unspecified role bait bait pug binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2b and c VCP/p97 c-terminal synthetic peptide (C-10 peptide) abolished the interaction. Incubation with a phosphorylated C-10 peptide had no effect, suggesting that interacting VCP/p97 is not phosphorylated. P55072 purified ubxn6-vcp-nsfl1c 777036 n/a 777042 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 777040 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 777038 unspecified role unspecified role prey prey physical association physical association false false false 2d P55072; O35987 purified vcp-ubxn6-1 777037 n/a 777040 unspecified role unspecified role neutral component neutral component n/a 777038 unspecified role unspecified role neutral component neutral component physical association physical association false false false 3b