IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ xing-2008-1 Arabidopsis PCFS4, a homologue of yeast polyadenylation factor Pcf11p, regulates FCA alternative processing and promotes flowering time. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Xing D., Zhao H., Xu R., Li QQ. liq@muohio.edu Plant J. (0960-7412) 2008 xing-2008-2 Arabidopsis PCFS4, a homologue of yeast polyadenylation factor Pcf11p, regulates FCA alternative processing and promotes flowering time. arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. tap tandem affinity purification TAP TAP tap western blot western blot Immuno blot Immuno blot Xing D., Zhao H., Xu R., Li QQ. liq@muohio.edu Plant J. (0960-7412) 2008 clp1_arath Protein CLP1 homolog CLPS3 CLP-like protein 3 Protein CLP-SIMILAR PROTEIN 3 F7O18.15 At3g04680 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAYGGPSMNPPALSGAVPGSANLKQVKLERESELRIEVSEEPLRLRVVNGTAEIFGSELPPEIWRTFPPRMKFAVFTWYGATIEMDGVTETDYTADETPMVSYINVHAILDARRRFAKASTSNDPESSQGPRVIVVGPTDSGKSTLTKMLLSWAAKQGWRPTFVDLDVGQGSITIPGSIAAAPIEMPLDPVEGFPLDMALVYYYGHASPNMNVELYKALVKELAQVLEKQFVGNPESRAAGMVINTMGWIEGIGYELLLHAIDTFNASVVLVLGQEKLFSRLKDVLRSKSNVDVVKLHKSGGVVARVKEVRKRSRNFKIQEYFYGLSKELSPYANTSSFSDLQVFRIGGGPQAPKSALPAGSTSVSNPLRVTPVNIDDRDLLHSVLAVSYAEEPDQIISSNVSGFVYVTEVNVQKKKITYLAPSPGTLPSKLLVAGSLAWLESV pcfs4_arath Polyadenylation and cleavage factor homolog 4 PCFS4 T4B21.1 At4g04885 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDSEKILNPRLVSINSTSRKGMSVELPQKPPPPPSLLDRFKALLNQREDEFGGGEEVLPPSMDEIVQLYEVVLGELTFNSKPIITDLTIIAGEQREHGEGIANAICTRILEAPVEQKLPSLYLLDSIVKNIGRDYGRYFSSRLPEVFCLAYRQAHPSLHPSMRHLFGTWSSVFPPPVLRKIDMQLQLSSAANQSSVGASEPSQPTRGIHVNPKYLRRLEPSAAENNLRGINSSARVYGQNSLGGYNDFEDQLESPSSLSSTPDGFTRRSNDGANPSNQAFNYGMGRATSRDDEHMEWRRKENLGQGNDHERPRALIDAYGVDTSKHVTINKPIRDMNGMHSKMVTPWQNTEEEEFDWEDMSPTLDRSRAGEFLRSSVPALGSVRARPRVGNTSDFHLDSDIKNGVSHQLRENWSLSQNYPHTSNRVDTRAGKDLKVLASSVGLVSSNSEFGAPPFDSIQDVNSRFGRALPDGTWPHLSARGPNSLPVPSAHLHHLANPGNAMSNRLQGKPLYRPENQVSQSHLNDMTQQNQMLVNYLPSSSAMAPRPMQSLLTHVSHGYPPHGSTIRPSLSIQGGEAMHPLSSGVLSQIGASNQPPGGAFSGLIGSLMAQGLISLNNQPAGQGPLGLEFDADMLKIRNESAISALYGDLPRQCTTCGLRFKCQEEHSKHMDWHVTKNRMSKNHKQNPSRKWFVSASMWLSGAEALGAEAVPGFLPTEPTTEKKDDEDMAVPADEDQTSCALCGEPFEDFYSDETEEWMYKGAVYMNAPEESTTDMDKSQLGPIVHAKCRPESNGGDMEEGSQRKKMRS fy_arath Flowering time control protein FY FY T6I14.10 At5g13480 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MYAGGDMHRGSQMPQPPMMRQSSASSTNINPDYHHPSGPFDPNVDSFGAKRMRKHTQRRAVDYTSTVVRYIQARTWQRDSRDRTTLQPTPAAAVDMLPTVAYSDNPSTSFAAKFVHASLNKNRCSINRVLWTPSGRRLITGSQSGEFTLWNGQSFNFEMILQAHDQPIRSMVWSHNENYMVSGDDGGTLKYWQNNMNNVKANKTAHKESIRDLSFCKTDLKFCSCSDDTTVKVWDFTKCVDESSLTGHGWDVKSVDWHPTKSLLVSGGKDQLVKLWDTRSGRELCSLHGHKNIVLSVKWNQNGNWLLTASKDQIIKLYDIRTMKELQSFRGHTKDVTSLAWHPCHEEYFVSGSSDGSICHWIVGHENPQIEIPNAHDNSVWDLAWHPIGYLLCSGSNDHTTKFWCRNRPADNPRDVLMQNQGYNEQGFGRQPDNFQPSEASPIPGAFVPGLTRNEGTIPGIGIAMPFDASSQGDHKQPLPGSMALGAPPLPPGPHPSLLGSGQQQGYQQQQQHQGHPQQMLPMPNMPHHQLPPSSHMPLHPHHLPRPMQMPPHGHMPPPSMPMSHQMPGSMGMQGGMNPQMSQSHFMGAPSGVFQGQPNSGGPQMYPQGRGGFNRPQMIPGYNNPFQQQQQPPLPPGPPPNNNQQHQ at4g04885-at3g04680 At4g04885 (PCFS4) interacts with At3g04680 (CLPS3) 745347 N/A 745349 unspecified role unspecified role prey prey N/A 745350 unspecified role unspecified role bait bait physical association physical association false false false Figure 5a at4g04885-at3g04680- At4g04885 (PCFS4) interacts with At3g04680 (CLPS3) and At5g13480 (FY) 745348 N/A 745351 unspecified role unspecified role prey prey N/A 745350 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 745349 unspecified role unspecified role prey prey association association false false false Figure 5b