IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ laporte-2008-3 Molecular and structural basis of cytokine receptor pleiotropy in the interleukin-4/13 system. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined LaPorte SL., Juo ZS., Vaclavikova J., Colf LA., Qi X., Heller NM., Keegan AD., Garcia KC. The ranges of the proteins and cell type used for expression of proteins were found in the pdb file for crystal structure of the complexes. kcgarcia@stanford.edu Cell (0092-8674) 2008 Only protein-protein interactions 10 imex curation laporte-2008-1 Molecular and structural basis of cytokine receptor pleiotropy in the interleukin-4/13 system. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined LaPorte SL., Juo ZS., Vaclavikova J., Colf LA., Qi X., Heller NM., Keegan AD., Garcia KC. The ranges of the proteins and cell type used for expression of proteins were found in the pdb file for crystal structure of the complexes. kcgarcia@stanford.edu Cell (0092-8674) 2008 Only protein-protein interactions 10 imex curation laporte-2008-2 Molecular and structural basis of cytokine receptor pleiotropy in the interleukin-4/13 system. trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined LaPorte SL., Juo ZS., Vaclavikova J., Colf LA., Qi X., Heller NM., Keegan AD., Garcia KC. The ranges of the proteins and cell type used for expression of proteins were found in the pdb file for crystal structure of the complexes. kcgarcia@stanford.edu Cell (0092-8674) 2008 Only protein-protein interactions 10 imex curation il4_human Interleukin-4 IL4 B-cell stimulatory factor 1 Lymphocyte stimulatory factor 1 Binetrakin Pitrakinra protein protein human Homo sapiens Human MGLTSQLLPPLFFLLACAGNFVHGHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS il2rg_human Cytokine receptor common subunit gamma IL2RG p64 gammaC Interleukin-2 receptor subunit gamma protein protein human Homo sapiens Human MLKPSLPFTSLLFLQLPLLGVGLNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQCFVFNVEYMNCTWNSSSEPQPTNLTLHYWYKNSDNDKVQKCSHYLFSEEITSGCQLQKKEIHLYQTFVVQLQDPREPRRQATQMLKLQNLVIPWAPENLTLHKLSESQLELNWNNRFLNHCLEHLVQYRTDWDHSWTEQSVDYRHKFSLPSVDGQKRYTFRVRSRFNPLCGSAQHWSEWSHPIHWGSNTSKENPFLFALEAVVISVGSMGLIISLLCVYFWLERTMPRIPTLKNLEDLVTEYHGNFSAWSGVSKGLAESLQPDYSERLCLVSEIPPKGGALGEGPGASPCNQHSPYWAPPCYTLKPET il4ra_human Interleukin-4 receptor subunit alpha IL4R 582J2.1 IL4RA protein protein human Homo sapiens Human MGWLCSGLLFPVSCLVLLQVASSGNMKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQHLLLGVSVSCIVILAVCLLCYVSITKIKKEWWDQIPNPARSRLVAIIIQDAQGSQWEKRSRGQEPAKCPHWKNCLTKLLPCFLEHNMKRDEDPHKAAKEMPFQGSGKSAWCPVEISKTVLWPESISVVRCVELFEAPVECEEEEEVEEEKGSFCASPESSRDDFQEGREGIVARLTESLFLDLLGEENGGFCQQDMGESCLLPPSGSTSAHMPWDEFPSAGPKEAPPWGKEQPLHLEPSPPASPTQSPDNLTCTETPLVIAGNPAYRSFSNSLSQSPCPRELGPDPLLARHLEEVEPEMPCVPQLSEPTTVPQPEPETWEQILRRNVLQHGAAAAPVSAPTSGYQEFVHAVEQGGTQASAVVGLGPPGEAGYKAFSSLLASSAVSPEKCGFGASSGEEGYKPFQDLIPGCPGDPAPVPVPLFTFGLDREPPRSPQSSHLPSSSPEHLGLEPGEKVEDMPKPPLPQEQATDPLVDSLGSGIVYSALTCHLCGHLKQCHGQEDGGQTPVMASPCCGCCCGDRSSPPTTPLRAPDPSPGGVPLEASLCPASLAPSGISEKSKSSSSFHPAPGNAQSSSQTPKIVNFVSVGPTYMRVS i13r1_human Interleukin-13 receptor subunit alpha-1 Cancer/testis antigen 19 IL13RA1 IL13R IL13RA protein protein human Homo sapiens Human MEWPARLCGLWALLLCAGGGGGGGGAAPTETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIAPETRRSIEVPLNERICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAVTELQCIWHNLSYMKCSWLPGRNTSPDTNYTLYYWHRSLEKIHQCENIFREGQYFGCSFDLTKVKDSSFEQHSVQIMVKDNAGKIKPSFNIVPLTSRVKPDPPHIKNLSFHNDDLYVQWENPQNFISRCLFYEVEVNNSQTETHNVFYVQEAKCENPEFERNVENTSCFMVPGVLPDTLNTVRIRVKTNKLCYEDDKLWSNWSQEMSIGKKRNSTLYITMLLIVPVIVAGAIIVLLLYLKRLKIIIFPPIPDPGKIFKEMFGDQNDDTLHWKKYDIYEKQTKEETDSVVLIENLKKASQ il13_human Interleukin-13 IL13 NC30 protein protein human Homo sapiens Human MHPLLNPLLLALGLMALLLTTVIALTCLGGFASPGPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGRFN il4-il4r-3 ITC reveals formation of the IL-4Ralpha/gammac/IL-4 ternary signaling complex 728675 N/A 728678 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. N/A 728679 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. N/A 728680 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false 3 A, Table S3 IL-4 first binds to IL-4Ra, followed by recruitment of gammac to form a ternary complex. The Kd of IL-4 binding to IL-4Ralpha = 1.0 E-9 M; the Kd of gammac binding to IL-4/IL-4Ralpha = 5.59 E-7 M. il4-il4r-4 ITC reveals formation of the IL-4Ra/IL-13Ra1/ IL-4 ternary signaling complex 728675 N/A 728681 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. N/A 728678 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. N/A 728680 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false 3 B, Table S3 For the type II IL-4 complex, IL-4 first binds to IL-4Ra followed by recruitment of IL-13Ralpha1. Kd for binding of IL-4 to IL-4Ralpha = 1.0 E-9 M; Kd of binding of IL-13Ralpha1 to IL-4/IL-4Ralpha = 4.87 E-7 M il13-il13ra1-2 ITC reveals formation of the IL-4Ralpha/IL-13Ralpha1/IL-13 ternary signaling complex 728675 N/A 728681 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. N/A 728682 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. N/A 728680 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false 3 C, Table S3 For the IL-13 complex, IL-13 first binds IL-13Ralpha1 and this binary complex then recruits IL-4Ralpha. Kd for IL-13 binding to IL-13Ralpha1 = 3.0 E-8 M; Kd for binding of IL-4Ralpha to IL-13/IL-13Ralpha1 = 2.0 E-8 il4-il4r-5 ITC reveals formation of the IL-4Ra/ IL-4 binary complex 728675 N/A 728680 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. N/A 728678 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false 3 A, B, Table S3 Kd for binding of IL-4 to IL-4Ralpha = 1.0 E-9 M il13-il13ra1-3 ITC reveals formation of the IL-13Ralpha1/IL-13 binary complex 728675 N/A 728681 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. N/A 728682 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false 3 C, Table S3 Kd for IL-13 binding to IL-13Ralpha1 = 3.0 E-8 M il4-il4r Crystal structure of the type I IL-4Ralpha/gammac/IL-4 ternary signaling complex 728676 N/A 728679 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 728678 unspecified role unspecified role neutral component neutral component c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 728680 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false S1 and S2, Table S1 2.95 A R free = 29.7; R cryst = 22.6 il4-il4r-2 Crystal structure of the type II IL-4Ra/IL-13Ra1/ IL-4 ternary signaling complex 728676 N/A 728681 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 728680 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 728678 unspecified role unspecified role neutral component neutral component c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false S1, S2, Table S1 3.0 A R free = 30.4; R cryst = 23.8 il13-il13ra1 Crystal structure of the type II IL-4Ralpha/IL-13Ralpha1/IL-13 ternary signaling complex 728676 N/A 728681 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 728680 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 728682 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false S1, S2, Table S1 il13-il13ra1-1 Gel filtration reveals interaction of ectodomain of IL-13Ralpha1 and IL-13 728677 N/A 728681 unspecified role unspecified role neutral component neutral component region Ectodomain D1 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 728682 unspecified role unspecified role neutral component neutral component physical association physical association false false false S5 A, B The IL-13Ralpha1 D1 domain is required for IL-13 binding. il4-il13ra1-2 Gel filtration reveals interaction of ectodomain of IL-13Ralpha1 and IL-4 728677 N/A 728681 unspecified role unspecified role neutral component neutral component region Ectodomain D2D3 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 728678 unspecified role unspecified role neutral component neutral component physical association physical association false false false S5 C The IL-13Ralpha1 D1 domain is required for IL-13 binding but not IL-4 binding.