UniProt UniProt http://www.uniprot.org wu-2007a-1 PAR-3 and PAR-1 inhibit LET-99 localization to generate a cortical band important for spindle positioning in Caenorhabditis elegans embryos. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wu JC., Rose LS. lsrose@ucdavis.edu Mol. Biol. Cell (1059-1524) 2007 Only protein-protein interactions imex curation wu-2007a-2 PAR-3 and PAR-1 inhibit LET-99 localization to generate a cortical band important for spindle positioning in Caenorhabditis elegans embryos. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wu JC., Rose LS. lsrose@ucdavis.edu Mol. Biol. Cell (1059-1524) 2007 Only protein-protein interactions imex curation par1_caeel Serine/threonine-protein kinase par-1 par-1 H39E23.1 protein protein caeel Caenorhabditis elegans MSSASVGKKPEHVNTPPGDNQQKSSKENQKSNKDKQSSNQPPGQIPHSTNSGTRKSSGSGLKTANLKHPARPSTESSTSSSHHRRPAQEMNGTSTATATGGGGTSGATTTASSGAPAASSGGSSARYSSSGRSHPTSGSSSSHARSTGQSGMSSRSAARRNDQDVHVGKYKLLKTIGKGNFAKVKLAKHVITGHEVAIKIIDKTALNPSSLQKLFREVKIMKQLDHPNIVKLYQVMETEQTLYLVLEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYLHSKNIIHRDLKAENLLLDQDMNIKIADFGFSNTFSLGNKLDTFCGSPPYAAPELFSGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLVINPQRRSSLDNIMKDRWMNVGYEDDELKPFIEPPKDQIDEQRIEKLIQIFQLGFNKAAILESVEKEKFEDIHATYLLLGERKSDMDASEITMAQSLLSHSSINVSSSLGQHPAGVITREHVTSSSASGSSASPSRYSRSSATATGASITAGSALASAANAQKHQQSSAAPSSGSSSSRRSSQNDAAATAAGGTVVMSGTRHGGVQMRAQPTSRQATISLLQPPSYKPSSNTTQIAQIPPLFNRNSTATSSAAQPSTGITGTRKIADPKGRIPLNSTAVQGHRTATGAVAANNGGIPSHRDHAQQQQYMNQLTSSTMMSKLINKTPAAGGTAATSSSSSSSATSTAPLQKSGSQISHAPTEPVIREDDDENNSENQNGNVPLIGGVGPQTSPAVQVPTEDATSSSDKEQQQQKASSETPKESKPSMIHQSPSMPPSQMMTAMESLKLSESGQTGGPTVATGGPPQRATSQQMSRSATTNSANMGASSGGAAAAASATNQLSGAPSSTGASSQQYHPKAPSSSSSSSTNPPHQHQLTHNASFSVTPSSYQIPTSTAVNVTSTGMPTSSSSSAFPRNTRNRQTFHGKTEKDKGGDDSSDEIGETPGNVSIGATGPSANNAEATIWSKLSKLTRRDHNRESMTQPVSGRAGTIGASQGQQTAAALAAIREQSGPIAPGAGQVAPSLPIHEGDVKPRSLRFTWSMKTTSSLAPDDMMREIRKVLDANGCDYEQRERYMILCVHGDPNTDSLVQWEMEVCKLPRLSLNGVRFKRISGTSIGFKNIASKIAQELNL let99_caeel Protein let-99 let-99 Lethal protein 99 K08E7.3 protein protein caeel Caenorhabditis elegans MSADYSSEKFKATHLFDEILWHFRSNLSLKTNRRGLATAENSFSGKEAVDFLMIEMPRIIPNNVPERDKMQKFLEFMMDMNVISEAFPKKVKQRRPFSESRIYLFMKTLDELKHPKPRSRRSASFSGARKSAKVAQPASPAATMHRPPKARLPRRLSRSNGNIDKAGIDNSSGGVENHGFDDHKDDEIPKKQRTPKILNRSLESICTEEYTEKREVSEMKEKVYDWLPFFKSRRNHTKVNQPTRRSASLDRNHCVLEQEKAEAALRSQKVTTPPIREAPKDVVFMHPQGPLPAVPSRYQNHRTSIAGSNPALLSRGRMYESIMRRSSVVPVADSVQANNECSFWKTELLGRLEQIYDRTLPCEWASKVDGYDIQWNMIEIDHADGIVKSRCQGLQPDYPQTVIQFMDYLVRYPFVTHKKMDTGLEYNVNRIFITLVNRLEDLNAPLQFDECSLIVNLLCKIDSFAAMLDNGPARRWSKVMISSSASSIEEAGLMVDGFSRDLPACGIRASKYRRRALSPFDNRVNLEIQDEKSYEIREQWLIEAIQLVLLSLPTSRRRKLHKFVTFIQSIETNAVFDLADPSNGSSNNREAAIIGLWTGVCSKCRKQQGMLITAVLLANFQSLFAVPVEFIEQVKRLECEEKDRYSGPRYAKITRSRNDWQPPVPDKPQASPAVFKKPLREVACEKTKKGLFTRLLRK let99-par1-1 765525 N/A 765527 unspecified role unspecified role prey prey N/A 765528 unspecified role unspecified role bait bait physical association physical association false false false Fig 6D let99-par1-2 765526 N/A-x 765528 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A-x 765527 unspecified role unspecified role prey prey caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. physical association physical association false false false Fig 6E