UniProt UniProt http://www.uniprot.org metcalf-2007-1 Role of SP65 in assembly of the Dictyostelium discoideum spore coat. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Metcalf T., van der Wel H., Escalante R., Sastre L., West CM. Cwest2@ouhsc.edu Eukaryotic Cell (1535-9778) 2007 sp65_dicdi Spore coat protein SP65 cotE SP65 DDB_G0277903 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MKVLLLLVCLVFAYVNASYDACYNVVCPSNYQCRAEGDQAYCVPEHHEYGCDRHSCGRGYECVERWDSFCCKPIHHRPHPHPRPPHPHPRPTNCDYTSCPREFDCHVINRNVTACLPDNHVCRDFQCPVGTHCFNGERGPHCVSDTHYPNLCRVTKCSYDFTCKMVRGNPTCLRNHDGISTHSPTHTPTHSPTTSPTHCPSCTELAPFCHSAGLVCRTILNPSCVSAVRGIINTRSCCTYIAVCASNSTSTTGATTGATTPTSTTGAATTAAATTTTATSTTGAATTAPATTTTTSTTGAATTAPATTTTSTTGAATTAPATTTTTATTIVGVTTANSVGGLTTTRATTVAGVTTANSVGGLTTAGITTVAGATTGNSVGGLTTARATTVADVTTANSVGGLTTAGVTTVAGATTGNSVGGLTTARATTVADVTTANSVGGLTTSRATTIAGATTANSIGGLTN sp85_dicdi Spore coat protein SP85 pspB PsB SP85 Cellulose-binding protein SP85 Prespore protein B Prespore protein 14E6 DDB_G0276939 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MRLLSVLLIGFLCLAGTYAQKYQLSPAYNDPYLTDDKTGTHDFWVQNASLPVFYGFHDWNFQDNSGIMEINGDEMHITGKIYPTVNMGDCHRYNVDLVFKKDKSGNVMPKKELRESAYVPHGPIDPATWKYYTFVQGKWTGFGCDPQNVVFSGAEGGMPLQLGYGANGKNGDNGISVWLIYGYTIVDINCNIRPIITQSPTQPPTQPPTYPPTQPPTQPPTQPPTYPPTYPPTYPPTYPPTYPPTHPPTYPPTYPPTQPPTQPPVQDCSTLECPEGFHCEIVNNRRTCVCDTTVPPTHPPTQSPTYPPTQPPTQPPTYPPTYPPTYPPTQPPRASCDNVRCPRGYHCECNHWENVARCVRNEEPTHRPKPPHHGCRPDSCARGEKCICVRGKIYCIPKTTCDQVRCPRKHHCECNRKGQVFCVPDCPKLTCKQVGCPENHECVSRRGELHCVYVRPPTGRWGDDLSDLGYNQASVEAAIDAYEAQRGQRPHNNIPSNNLHQENNDNLGDGFYDGVEIGFADGGFDVNDLNGF cote-pspb-1 764575 N/A 764576 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain greater-than greater-than false N/A 764577 unspecified role unspecified role prey prey physical association physical association false false false Fig 10 Fragmentation of cotE assumed by authors from mol. wt.