UniProt UniProt http://www.uniprot.org mendoza-2007-1 MEK1 and protein phosphatase 4 coordinate Dictyostelium development and chemotaxis. dicdi Dictyostelium discoideum (Slime mold) Slime mold anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mendoza MC., Booth EO., Shaulsky G., Firtel RA. rafirtel@ucsd.edu Mol. Cell. Biol. (0270-7306) 2007 Only protein-protein interactions imex curation smek_dicdi Suppressor of Mek1 smkA DDB_G0289067 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MEPLRKRVKVYQLDNSGKWDDKGTGHVSCIYVDALCAMGLIVRSESDNSVILQTRLSAEDIYQKQQDSLIVWTEPDSQLDLALSFQDSLGCQDIWENILQYQNQRTGSCDSVDLDLPPVSINNLQTINELLEASLPMLDKDKIINSIFKEDLVRSLLDLFDEIEKSGEGGVHLFQIFNIFKNLILFNDTSILEVILSEDYLVRVMGALEYDPEISENNRIKHREFLNQQVVFKQVIKFPSKSLIGTIHQTFRIQYLKDVVLPRVLDDVTFSSLNSLIYFNNIDIVSQIQNDSDFLENLFSEIQKSEKNSEERKDLILFLQDLCNLAKGLQIQSKSTFFTVVVSLGLFKTLSAILDDENVQTRVSCTEIVLSTLLHDPEILRSYLCSPTSGNSKFLVQLINLFITDKDIGVKNQIVEIIKTLLEADSYDSSDFFRLFYDKGIDLLVSPLNEVYKGEPTIPGDPSSNLDSFVLYNIMELVIYCIKHHCYRIKHFIVEEGIAKKILRYTNPTGSGGGGGGGGNSERYLILGSIRFFRSMVNMKDDLYNQHIIQENLFEPIIEVFKSNISRYNLLNSAIIELFQYIYKENIRDLIVYLVERYRELFESVTYTDVLKQLILKYEQIKDSSFESPETSCNNNDSSSNDIDSKPIIGNNKINHNYQRTQREIDEEEEEAYFNRDDDSEDSDDEDELIPISINNNNNNNNNNKQICTNNENNMEKNDDNIEKDNENTNNGNGSSHIKIVDYEDEDDEDDEINKSVESDDIVEKHEIIDKNEKKDEIMKENNDSDNDDNDNNDNDNDNDNNSDIENKNHLNNNGNNENNENNDDVQDKSNNKNNSDKINEDEKIEKQDEMKENLEMEEIDEKVKEKQPKDIKKENQSQPDETVFNGKSNNSNNNNNNNNNNSNNQEIGDNRKTTPKRKLDYEKNESVVSKKIDKSNGPTSIDKDINGCDESPNKKLNNNNSNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNQNDENELSSASEEEEEQLENGKHIKKFKRGKKDSNNSSNNSNNSSPTPSELHV pp4c_dicdi Serine/threonine-protein phosphatase 4 catalytic subunit ppp4c pppC DDB_G0272116 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MSSDLDRQIEQLKRCEIIKESEVRALCSKAREILLEEGNVQRVDSPVTICGDIHGQFYDLKELFKVGGDCPQTNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYEECVRKYGSVTVWKYCTEIFDYLSLSALVDGKIFCVHGGLSPSINTLDQIRAIDRKQEVPHEGPMCDLMWSDPEDIPGWNGSPRGAGFLFGEDVVQKFNHDNNLEFICRAHQLVMEGFKYMFNETLVTVWSAPNYCYRCGNVAAILQLDENLKKNFAIFEAAPQESRGAPAKKPAPEYFL ppp4c-smka 771387 n/a 771388 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 771389 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false The interaction was observed in vegetative and developmental cells (treated with cAMP pulsing). 1A ppp4c-smka-1 771387 n/a 771388 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 771389 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false The interaction was observed in vegetative and developmental cells (treated with cAMP pulsing). 1B HA-SMEKdeltaEVH1 and HA-EVH1 constructs used to determine the binding site are according PMID:16107728 (same labaratory).