IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kieffer-2006-2 Analysis of the transcription factor WUSCHEL and its functional homologue in Antirrhinum reveals a potential mechanism for their roles in meristem maintenance. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kieffer M., Stern Y., Cook H., Clerici E., Maulbetsch C., Laux T., Davies B. b.h.davies@leeds.ac.uk Arabidopsis cDNA library constructed from Arabidopsis shoot apices Plant Cell (1040-4651) 2006 Only protein-protein interactions imex curation kieffer-2006-1 Analysis of the transcription factor WUSCHEL and its functional homologue in Antirrhinum reveals a potential mechanism for their roles in meristem maintenance. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kieffer M., Stern Y., Cook H., Clerici E., Maulbetsch C., Laux T., Davies B. b.h.davies@leeds.ac.uk Arabidopsis cDNA library constructed from Arabidopsis shoot apices Plant Cell (1040-4651) 2006 Only protein-protein interactions imex curation kieffer-2006-3 Analysis of the transcription factor WUSCHEL and its functional homologue in Antirrhinum reveals a potential mechanism for their roles in meristem maintenance. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kieffer M., Stern Y., Cook H., Clerici E., Maulbetsch C., Laux T., Davies B. b.h.davies@leeds.ac.uk Arabidopsis cDNA library constructed from Arabidopsis shoot apices Plant Cell (1040-4651) 2006 Only protein-protein interactions imex curation wus_arath Protein WUSCHEL WUS PGA6 Plant growth activator 6 T27K22.18 At2g17950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEPPQHQHHHHQADQESGNNNNNKSGSGGYTCRQTSTRWTPTTEQIKILKELYYNNAIRSPTADQIQKITARLRQFGKIEGKNVFYWFQNHKARERQKKRFNGTNMTTPSSSPNSVMMAANDHYHPLLHHHHGVPMQRPANSVNVKLNQDHHLYHHNKPYPSFNNGNLNHASSGTECGVVNASNGYMSSHVYGSMEQDCSMNYNNVGGGWANMDHHYSSAPYNFFDRAKPLFGLEGHQEEEECGGDAYLEHRRTLPLFPMHGEDHINGGSGAIWKYGQSEVRPCASLELRLN tpl_arath Protein TOPLESS TPL WSIP1 WUS-interacting protein 1 F7H2.9 At1g15750 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSLSRELVFLILQFLDEEKFKETVHKLEQESGFFFNMKYFEDEVHNGNWDEVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDKHDRPKAVDILVKDLKVFSTFNEELFKEITQLLTLENFRENEQLSKYGDTKSARAIMLVELKKLIEANPLFRDKLQFPTLRNSRLRTLINQSLNWQHQLCKNPRPNPDIKTLFVDHSCGPPNGARAPSPVNNPLLGGIPKAGGFPPLGAHGPFQPTASPVPTPLAGWMSSPSSVPHPAVSAGAIALGGPSIPAALKHPRTPPTNASLDYPSADSEHVSKRTRPMGISDEVNLGVNMLPMSFSGQAHGHSPAFKAPDDLPKTVARTLSQGSSPMSMDFHPIKQTLLLVGTNVGDIGLWEVGSRERLVQKTFKVWDLSKCSMPLQAALVKEPVVSVNRVIWSPDGSLFGVAYSRHIVQLYSYHGGEDMRQHLEIDAHVGGVNDISFSTPNKQLCVITCGDDKTIKVWDAATGVKRHTFEGHEAPVYSVCPHYKENIQFIFSTALDGKIKAWLYDNMGSRVDYDAPGRWCTTMAYSADGTRLFSCGTSKDGESFIVEWNESEGAVKRTYQGFHKRSLGVVQFDTTKNRYLAAGDDFSIKFWDMDAVQLLTAIDGDGGLQASPRIRFNKEGSLLAVSGNENVIKIMANSDGLRLLHTFENISSESSKPAINSIAAAAAAAATSAGHADRSANVVSIQGMNGDSRNMVDVKPVITEESNDKSKIWKLTEVSEPSQCRSLRLPENLRVAKISRLIFTNSGNAILALASNAIHLLWKWQRNERNATGKATASLPPQQWQPASGILMTNDVAETNPEEAVPCFALSKNDSYVMSASGGKISLFNMMTFKTMATFMPPPPAATFLAFHPQDNNIIAIGMDDSTIQIYNVRVDEVKSKLKGHSKRITGLAFSNVLNVLVSSGADAQLCVWNTDGWEKQRSKVLPLPQGRPNSAPSDTRVQFHQDQAHFLVVHETQLAIYETTKLECMKQWAVRESLAPITHATFSCDSQLVYASFMDATVCVFSSANLRLRCRVNPSAYLPASLSNSNVHPLVIAAHPQEPNMFAVGLSDGGVHIFEPLESEGKWGVAPPAENGSASGAPTAPSVGASASDQPQR tpr4_arath Topless-related protein 4 TPR4 WSIP2 WUS-interacting protein 2 MSJ11.27 At3g15880 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSLSRELVFLILQFLDEEKFKDTVHRLEKESGFFFNMRYFEDSVTAGEWDDVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDKKDHAKAVDILVKELKVFSTFNEELFKEITMLLTLTNFRENEQLSKYGDTKSARGIMLGELKKLIEANPLFRDKLQFPSLKNSRLRTLINQSLNWQHQLCKNPRPNPDIKTLFVDHTCGHPNGAHTPSPTTNHLMGSVPKVGGFPPLGAHGPFQPTPAPLTTSLAGWMPNPSVQHPTVSAGPIGLGAPNSAVSMLKRERPRSPPTNSLSMDYQTADSESVLKRPRPFGISDGVNNLPVNVLPVTYPGQSHAHATYSTDDLPKNVSRILSQGSAIKSMDFHPVQQTMLLVGTNLGDIAIWEVGSREKLVSRSFKVWDLATCTVNLQASLASEYTAAVNRVVWSPDGGLLGVAYSKHIVHIYSYHGGEDLRNHLEIDAHAGNVNDLAFSQPNQQLCVVTCGEDKTIKVWDAVTGNKLHTFEGHEAPVYSVCPHQKENIQFIFSTAVDGKIKAWLYDNMGSRVDYDAPGRSCTSMAYCADGTRLFSCGTSKEGESFIVEWNESEGAVKRTYLGLGKRSVGVVQFDTMKNKFLVAGDEFQVKFWDMDSVDLLSSTAAEGGLPSSPCLRINKEGTLLAVSTTDNGIKILANAEGSRILHSMANRGLDSSRAPPGSVAKGPIVGTFGTPNSSTGMSLSMGERSGPVASVTGLNGDNRSLPDVKPRIADDAEKSKTWKLTEISERSQLRTLRLPDTLLPARVVKLIYTNSGGAILALAENAAHKLWKWQKSERNLLGKANSNVPPQLWQPSSGVLMTNDTREGNKEDVVPCFALSKNDSYVMSASGGKISLFNMMTFKTMTTFMAPPPAATSLAFHPQDNNIIAIGMDDSSIQIYNVRVDEVKSKLKGHQKRVTGLAFSNVLNVLVSSGADSQLCVWSMDGWEKQASKQIQIPSGHSPNPLAHTRVQFHQDQIHVLVVHASQLAIYEAPKLENMKQWIPKESSGSVTDAVYSCDSQSIYAAFDDGSVSILTATTLQLKCRIGPNSYLPSNPSSRVYPATVAAHPSEPNQFAVGLTDGGVHVIEPPGPEGKWGISAPPENGAGPSVSSAPGSDQQPR wus_pethy Protein WUSCHEL WUS TER PhWUS Protein TERMINATOR protein protein pethy Petunia hybrida (Petunia) METAQHQQNNQQHYLHQHLSIGQGTNIEDGSNKNNSSNFMCRQNSTRWTPTTDQIRILKDLYYNNGVRSPTAEQIQRISAKLRQYGKIEGKNVFYWFQNHKARERQKKRLIAAATTDNTNLPMQMQFQRGVWRSSADDPIHHKYTNPGVHCPSASSHGVLAVGQNGNHGYGALAMEKSFRDCSISPGSSMSHHHHQNFAWAGVDPYSSTTTYPFLEKTKHFENETLEADEEQQEEDQENYYYQRTTSAIETLPLFPMHEENISSFCNLKHQESSGGFYTEWYRADDNLAAARASLELSLNSFIGNSS wus-wisp1-1 780464 n/a 780467 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind range range range range false n/a 780468 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Figure 6 Note the authors state that the prey protein was truncated but fail to provide amino-acid coordinates ; "in both cases, the cDNAs isolated in the two-hybrid screen were found to be truncated". The data presented in Figure 6A indicates that the interacting region is truncated before the WD repeat region of the protein. The region annotated represents this region , the coordinates were taken from the UniProt record and are presented as fuzzy coordinates to indicate that the coordinates are not explictly stated in the paper (see the IntAct Annotation manual available at the IntAct website for further details). Likewise the region of WUS interacting with WISP1 was annotated in the same way, in this case Figure 6A indicates WUS was truncated after the homeodomain region of the bait protein. wus-wisp2-1 780464 n/a 780469 unspecified role unspecified role prey prey n/a 780467 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind range range range range false physical association physical association false false false Figure 6 Note the authors state that the prey protein was truncated but fail to provide amino-acid coordinates ; "in both cases, the cDNAs isolated in the two-hybrid screen were found to be truncated". Details in Figure 6A indicate that the WUS protein was truncated after the homeodomain region, the coordinates for the truncated protein were taken from the UniProt record and presented as fuzzy coordinates to indicate the coordinates were not explicitly stated in the text of the paper (for further details see the IntAct Annotation manual available at the IntAct website). ros-wisp1 780464 n/a 780470 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind range range range range false n/a 780468 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Figure 6 Note the authors state that the prey protein was truncated but fail to provide amino-acid coordinates ; "in both cases, the cDNAs isolated in the two-hybrid screen were found to be truncated". The data presented in Figure 6A indicates that the interacting region is truncated before the WD repeat region of the protein. The region annotated represents this region , the coordinates were taken from the UniProt record and are presented as fuzzy coordinates to indicate that the coordinates are not explictly stated in the paper (see the IntAct Annotation manual available at the IntAct website for further details). Likewise the region of WUS interacting with WISP1 was annotated in the same way, in this case Figure 6A indicates ROS was truncated after the homeodomain region of the bait protein. wus-wisp2 780465 n/a 780467 unspecified role unspecified role bait bait n/a 780469 unspecified role unspecified role prey prey physical association physical association false false false Figure 7C Note the authors state that the prey protein was truncated but fail to provide amino-acid coordinates ; "in both cases, the cDNAs isolated in the two-hybrid screen were found to be truncated". wus-wisp1 780465 n/a 780468 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 780467 unspecified role unspecified role bait bait physical association physical association false false false Figure 7C Note the authors state that the prey protein was truncated but fail to provide amino-acid coordinates ; "in both cases, the cDNAs isolated in the two-hybrid screen were found to be truncated". The data presented in Figure 6A indicates that the interacting region is truncated before the WD repeat region of the protein. The region annotated represents this region , the coordinates were taken from the UniProt record and are presented as fuzzy coordinates to indicate that the coordinates are not explictly stated in the paper (see the IntAct Annotation manual available at the IntAct website for further details). wus-wisp1-2 780466 n/a 780467 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 780468 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 6 Note the authors concluded the "results demonstrate that the interaction between WSIP1 and WUS requires the C-terminal conserved domain of WUS and the N-terminal LisH domain of WSIP1". As in previous entries the the cooordinates have been taken fronm the UniProt entries and presented as fuzzy coordinates as they are not explicitly stated in the text of the paper. The authors concluded the "results demonstrate that the interaction between WSIP1 and WUS requires the C-terminal conserved domain of WUS and the N-terminal LisH domain of WSIP1".