UniProt UniProt http://www.uniprot.org schirenbeck-2005-2 The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Schirenbeck A., Bretschneider T., Arasada R., Schleicher M., Faix J. faix@bpc.mh-hannover.de Nat. Cell Biol. (1465-7392) 2005 schirenbeck-2005-1 The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Schirenbeck A., Bretschneider T., Arasada R., Schleicher M., Faix J. faix@bpc.mh-hannover.de Nat. Cell Biol. (1465-7392) 2005 schirenbeck-2005-3 The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia. dicdi Dictyostelium discoideum (Slime mold) Slime mold anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Schirenbeck A., Bretschneider T., Arasada R., Schleicher M., Faix J. faix@bpc.mh-hannover.de Nat. Cell Biol. (1465-7392) 2005 rac1a_dicdi Rho-related protein rac1A rac1A DDB_G0277869 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSIISPSSFENVNGKWHPEICHHAPNVPIILVGTKLDMREDKETQDRLKEKKLYPISYEQGLAKMKEINAVKYLECSALTQKGLKTVFDEAIRAVINPPLSKKKKSSGGCNIL forh_dicdi Formin-H forH dia2 drf2 DDB_G0285589 Diaphanous-related formin dia2 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MSFDLESNSSGGSTIGRNSSIRLSSGLAPSESTVSLNEIIDLDREFELLLDKLAIEDPIKRKQMQSLPDISKRTLLEQNKADIYRTVKHKGPIESFADVKSVISSINTKHVPIDIIKTLRIHLNTADRDWIQSFLDNDGVQPILNILKRLERNKNRKRKEHSILQWECTRCIAALMKIKIGMEYIASFPQTTNLMVLCLDTPLIKAKTLVLELLAAIAVTDRGHGAVLTSMIYHKEVKKEITRYFNLVQSLKIEKNAEYLTTCMSFINCIISSPSDLPSRIEIRKAFLNLKILKYIENLRADYNEDKNLLTQLDVFEEELSTDEQLNSQQGTQIGIEDLFSQISSRVTGTPSQQELITLMTHFQRMSSSNLGLGVWTLYNALANQLEDELKIHPDLDVTLVSLLFPEVKKSSSGLFGFGSKSKSPSSSPALSSMAKTELKKDNEEKQKTIEHLLKQLNKFSGGQNTERWMIEREEKNKLIAQLMAQTKNGGGGGGGGVGGDSSLSNDEALKRENQLLRMEIENIKNNPSVLLNSGNSINGDVPNLFISSPGSTLSPSPSGEPPIPSTDFGITSSSIHTSTDKLTNSTEPILGSPPPPPPPPMSGGGGPPPPPPPPGGKSNKPAKPIIKPSVKMRNFNWITIPALKVQGTFWDKLDETSFIQSLDKVELESLFSAKAPTVKVESKQLTRKVVVTVIDMKKANNCAIMLQHFKIPNEQLKKMQIMLDEKHFSQENAIYLLQFAPTKEDIEAIKEYQGDQMQLGAAEQYMLTVMDIPKLDSRLKAFIFKQKFEGLVEDLVPDIKAIKAASLELKKSKRLSDILKFILAIGNYVNGSTTRGGAFGFKVLETLPKMRDARSNDNKLSLLHFLAKTLQDRIPEIWNIGAELPHIEHASEVSLNNIISDSSEIKRSIDLIERDFVPMINDPLFAHDKHWIHKITEFQKIAKVQYQRIEKEIDEMNKAFEEITSYFGEPKSTQPDVFFSTINNFLEDLEKAYGEYQAMIRKAELENSKMEDPEKGGLQDLSSQIRSGQLFKDRRVGDSVIAQMQNVDSLRKNLKSTSTTTPNTPPTIKIELPSQSILKPSGQLKK rgaa_dicdi Ras GTPase-activating-like protein rgaA rgaA DDB_G0287585 DG1029 rasGAP1 Developmental gene 1029 protein protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MNKEEYSDISDSESEEVHETNNHNEHEHEEEDDTPEIVVPERKFLKEDEDYSVPFPVMRECLVLLLQSRRILRDMMYYRFKMDRFLSGNLSVFEIQNLLHSQREDKESDWIAEIQELKRNLVSEVRRNHTLERDLNRLDKRIALLIKNRGNIQDVLADKAGLKAPKHKGDQKKPELINDPKKLEAYQNLFYLLQTEPKYLAGLVYLIQPEQMESFLGTVILTLFGDAFTPREEFLLLSLYRLSIQKEMANIATVGDFLKADTVVPKMIITYNKRKQGTDYLKAVIGPILSNVIKQELNLELKPNLVYAAIISEQEIRTGEKSTLDRNVSHEKALEVPEVTKTIKARVDQLISICEQFLDGIISSLNRLPYGIRWICKQIYQIAEKNFTKSTQDEILKVIGYFIYYRFIQVAMVSPEEYDLVGREIHPTARKNLINVSKVLQALFNFAQFGSSEKHFIPLNGWITSHMGDIKNYLQEIIEVGEPEDYLQVDKYMELTQKTKPVIIISLPEICNTHQLISKNLDSLVAKGEKDDPMRIIMKELDEFGPPPDIAADDDREVQLTLSNKFQKTIEEELSPGESLLSQTKEMVISLLRALPTLPEQKDQSDEPPNLVDVLNKARQADPSLEPEIKKILDNLKKLEEYNLTTSADNYSSFLKAVALEVVNRAEIREQQKKEKQRLTTSLNNLRKHQKYLNEQIAQYNQYLQDCRLKHYQNKSKKKKKGDGAKVGPFKFSFSELHKKGVIVDSEVPQITRKKIKFVISSDTVGVFDVSAKMAGIDVQTMRLELDDLLELNSIGTTTLELDQITLDVNMTIHLLNKLFLY prof2_dicdi Profilin-2 proB Profilin II DDB_G0286187 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MTWQAYVDNNLLGAGFASAALLGAADGSVWAHSAGFNVAEGKAITALFQKDGAAFATGIHVAGKKYMAIKSDTRSAYGKLGAGGVVCVKTLTCIIVAVYDDKLQPGAAANIAEKLADYLIDNNC rac1a-forh-1 764531 N/A 764534 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 764535 unspecified role unspecified role prey prey n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false dicdi Dictyostelium discoideum (Slime mold) Slime mold physical association physical association false false false Fig 1d Interaction between rac1A and forH is stimulated by GTP-gamma-S. rac1a-rgaa-1 764531 N/A 764536 unspecified role unspecified role prey prey dicdi Dictyostelium discoideum (Slime mold) Slime mold N/A 764534 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 1d Interaction between rac1A and rgaA is stimulated by GTP-gamma-S. prob-forh-1 764532 N/A 764537 unspecified role unspecified role bait bait N/A 764535 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1c forh-rac1a-1 764533 N/A-x 764534 unspecified role unspecified role prey prey n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P34144:p.Thr17Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P34144:p.Gly12Val mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A-x 764535 unspecified role unspecified role bait bait dicdi Dictyostelium discoideum (Slime mold) Slime mold physical association physical association false false false Fig 1e