IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nam-2004-3 The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Nam KH., Li J. jian@umich.edu Arabidopsis cDNA library. Plant Cell (1040-4651) 2004 nam-2004-1 The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nam KH., Li J. jian@umich.edu Arabidopsis cDNA library. Plant Cell (1040-4651) 2004 nam-2004-2 The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nam KH., Li J. jian@umich.edu Arabidopsis cDNA library. Plant Cell (1040-4651) 2004 bri1_arath Protein BRASSINOSTEROID INSENSITIVE 1 Brassinosteroid LRR receptor kinase BRI1 F23K16.30 At4g39400 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKTFSSFFLSVTTLFFFSFFSLSFQASPSQSLYREIHQLISFKDVLPDKNLLPDWSSNKNPCTFDGVTCRDDKVTSIDLSSKPLNVGFSAVSSSLLSLTGLESLFLSNSHINGSVSGFKCSASLTSLDLSRNSLSGPVTTLTSLGSCSGLKFLNVSSNTLDFPGKVSGGLKLNSLEVLDLSANSISGANVVGWVLSDGCGELKHLAISGNKISGDVDVSRCVNLEFLDVSSNNFSTGIPFLGDCSALQHLDISGNKLSGDFSRAISTCTELKLLNISSNQFVGPIPPLPLKSLQYLSLAENKFTGEIPDFLSGACDTLTGLDLSGNHFYGAVPPFFGSCSLLESLALSSNNFSGELPMDTLLKMRGLKVLDLSFNEFSGELPESLTNLSASLLTLDLSSNNFSGPILPNLCQNPKNTLQELYLQNNGFTGKIPPTLSNCSELVSLHLSFNYLSGTIPSSLGSLSKLRDLKLWLNMLEGEIPQELMYVKTLETLILDFNDLTGEIPSGLSNCTNLNWISLSNNRLTGEIPKWIGRLENLAILKLSNNSFSGNIPAELGDCRSLIWLDLNTNLFNGTIPAAMFKQSGKIAANFIAGKRYVYIKNDGMKKECHGAGNLLEFQGIRSEQLNRLSTRNPCNITSRVYGGHTSPTFDNNGSMMFLDMSYNMLSGYIPKEIGSMPYLFILNLGHNDISGSIPDEVGDLRGLNILDLSSNKLDGRIPQAMSALTMLTEIDLSNNNLSGPIPEMGQFETFPPAKFLNNPGLCGYPLPRCDPSNADGYAHHQRSHGRRPASLAGSVAMGLLFSFVCIFGLILVGREMRKRRRKKEAELEMYAEGHGNSGDRTANNTNWKLTGVKEALSINLAAFEKPLRKLTFADLLQATNGFHNDSLIGSGGFGDVYKAILKDGSAVAIKKLIHVSGQGDREFMAEMETIGKIKHRNLVPLLGYCKVGDERLLVYEFMKYGSLEDVLHDPKKAGVKLNWSTRRKIAIGSARGLAFLHHNCSPHIIHRDMKSSNVLLDENLEARVSDFGMARLMSAMDTHLSVSTLAGTPGYVPPEYYQSFRCSTKGDVYSYGVVLLELLTGKRPTDSPDFGDNNLVGWVKQHAKLRISDVFDPELMKEDPALEIELLQHLKVAVACLDDRAWRRPTMVQVMAMFKEIQAGSGIDSQSTIRSIEDGGFSTIEMVDMSIKEVPEGKL tthl_arath Uric acid degradation bifunctional protein TTL TTL Transthyretin-like protein MCK7.9 At5g58220 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAMEIGEDEWKVCCGSSEFAKQMSTSGPLTSQEAIYTARDIWFNQVNVTDWLEAFSAHPQIGNTPSPSINSDFARRSVSEQSTAFATTSASALQELAEWNVLYKKKFGFIFIICASGRTHAEMLHALKERYENRPIVELEIAAMEQMKITELRMAKLFSDKAKVISETDSSSSPVSTKPQDRLRIIGGHLNVAAEAKAPKRSRPPITTHVLDVSRGAPAAGVEVHLEVWSGTTGPSFVHGGGGVWSSVGTSATDRDGRSGPLMDLVDALNPGTYRISFDTAKYSPGCFFPYVSIVFQVTESQKWEHFHVPLLLAPFSFSTYRGS brl1-4 BRL1 protein autophosphorylation 761842 N/A 761845 putative self putative self putative self putative self O22476:p.Gly1048Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind range range range range false O22476:p.Glu1078Lys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false O22476:p.Ala1031Thr mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false true false The range of the kinase domain used in the yeast two hybrid assay was not described in the text. The range is taken from Li and Chroy (1997) , PMID PMID: 9298904. Because the range is not directly taken from the source text, it is presented as a fuzzy range to indicate that the range was not explicitly stated by the authors of the paper under curation. Figure 2C autophosphorylation of BRL1 protein brl1-tll BRL1 protein phosphorylation of TLL protein 761842 N/A 761846 enzyme target enzyme target substrate substrate neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 761845 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O22476:p.Glu1078Lys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false O22476:p.Gly1048Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false O22476:p.Ala1031Thr mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind range range range range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false The range of the kinase domain used in the yeast two hybrid assay was not described in the text. The range is taken from Li and Chroy (1997) , PMID PMID: 9298904. Because the range is not directly taken from the source text, it is presented as a fuzzy range to indicate that the range was not explicitly stated by the authors of the paper under curation. Figure 2C phosphorylation of TLL protein autophosphorylation of BRL1 protein brl1-ttl Interaction of BRL1 protein and the TTL protein 761843 N/A 761845 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 761846 unspecified role unspecified role prey prey physical association physical association false false false The range of the kinase domain used in the yeast two hybrid assay was not described in the text. The range is taken from Li and Chroy (1997) , PMID PMID: 9298904. Because the range is not directly taken from the source text, it is presented as a fuzzy range to indicate that the range was not explicitly stated by the authors of the paper under curation. brl1-ttl-1 Interaction of BRL1 protein and the TTL protein 761844 N/A 761846 unspecified role unspecified role prey prey n-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 761845 unspecified role unspecified role bait bait regio sufficient to bind sufficient binding region sufficient to bind range range range range false O22476:p.Gly1048Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false O22476:p.Glu1078Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The range of the kinase domain used in the yeast two hybrid assay was not described in the text. The range is taken from Li and Chroy (1997) , PMID PMID: 9298904. Because the range is not directly taken from the source text, it is presented as a fuzzy range to indicate that the range was not explicitly stated by the authors of the paper under curation. Table 1 Figure 2B It should be noted that when the n-terminal 29 amino acid region of the TTL protein, demonstrated to be critical for interaction with BRL1, was fused to a protein which does not usually interact with BRL1; no interaction occurred. The authors concluded that other regions, besides the n-termianl region of the TTL protein, facilitated interaction with BRL1.