UniProt UniProt http://www.uniprot.org yoshimura-2004-1 Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yoshimura M., Asai K., Sadaie Y., Yoshikawa H. hiyoshik@nodai.ac.jp Microbiology 150 (591-599) 2004 Only protein-protein interactions imex curation rsiv_bacsu Anti-sigma-V factor RsiV rsiV BSU27130 yrhM protein protein bacsu Bacillus subtilis MDKRLQQLREEYKNVQIPKELDIIVEKALQQEPKKKRIVMWPTSAAIAAAILFTALVNINPDAAQAMSKIPVIGKIVKAITFIEIKEEKDQSSIDVKTPALSGLSNKELENSINEKYLKESQQLYKEFIQSTSKNKKGHLSIYSDYETVTDTPDLLSIRRNIETTQASSYTQSRYITIDKKNDILLTLKSLFKDERYIKVISQNIKEQMKQQMKEDPNKIYWLTDEDAEPFKTILPDQTFYITEDHKLVISFDEYEVAPGYMGVTEFTIPTGVISNLLVGERYIR sigv_bacsu RNA polymerase sigma factor SigV sigV BSU27120 protein protein bacsu Bacillus subtilis MKKKQTTKALLVTCITDHKQDFYRLAFSYVKNQDDALDIVQESIKKALSSVETVRNPETIKSWFYKILVRTAIDFLRKQKKIRVMDDETIEFLSKGKEDHYKDTDLHEALDELPYRYKTIIILRFFEDLKLEEIAEITGENTNTVKTRLYRALKLMRIQLTKEDLS rsiw_bacsu Anti-sigma-W factor RsiW rsiW ybbM BSU01740 Regulator of SigW Sigma-W anti-sigma factor RsiW protein protein bacsu Bacillus subtilis MSCPEQIVQLMHMHLDGDILPKDEHVLNEHLETCEKCRKHFYEMEKSIALVRSTSHVEAPADFTANVMAKLPKEKKRASVKRWFRTHPVIAAAAVFIILMGGGFFNSWHNDHNFSVSKQPNLVVHNHTVTVPEGETVKGDVTVKNGKLIIKGKIDGDVTVVNGEKYMASAGQVTGQIEEINQLFDWTWYKMKSAGKSVLDAFNPNGEE sigw_bacsu ECF RNA polymerase sigma factor SigW sigW ybbL BSU01730 Alternative RNA polymerase sigma factor SigW RNA polymerase sigma-W factor protein protein bacsu Bacillus subtilis MEMMIKKRIKQVKKGDQDAFADIVDIYKDKIYQLCYRMLGNVHEAEDIAQEAFIRAYVNIDSFDINRKFSTWLYRIATNLTIDRIRKKKPDYYLDAEVAGTEGLTMYSQIVADGVLPEDAVVSLELSNTIQQKILKLPDKYRTVIVLKYIDELSLIEIGEILNIPVGTVKTRIHRGREALRKQLRDL rsix_bacsu Anti-sigma-X factor RsiX rsiX ypuN BSU23090 Sigma-X negative effector protein protein bacsu Bacillus subtilis MMKSEWNEEQIKELLSQLPAVKDHRSPQDIYKRLTMAKRKNKPAVRWIGPACAAAIAVYIAFIISPHFFDQAQPQQKEASQENAVTKTETEDSPKAASSLDQTSFVVPEKEQDNYITVAVADADTSAIIPVSIQKTNADQTIQDMLFESSELGILDHAITIPTFIDEVEIKEKPKQKELSIRVHQPATAFSIKDDTLLKKLLKESLKWSPYEKVKFLSDQNETGVRIGSYGTFTEISIPKQSKRSYYLYQNKQGQDFLVPSNHSFDTVKEAIKEMESSSQEDTTPLIQAGAVQSVTKKQKHLYIRFSKESEVDDSIAGILMIEGLLLTAKEFGFTEVTFTETRTKKIGKYDISDAIPVPAAPNPISLN sigx_bacsu ECF RNA polymerase sigma factor SigX sigX ypuM BSU23100 protein protein bacsu Bacillus subtilis MEETFQLLYDTYHQDLYQFLFYMVKDKNQTEDLLQEVYIRVLNSYHTFEGRSSEKTWLLSIARHVAIDWFRKQQTIRQRILGTFDWDTQDVRDQQLLPDELAVQHENVREIYAALDQCTIDQRAVIILRFIQGYSIQETAKALRFSESKVKTTQHRGLKVLRKHMELLREELMDDEVRMERRTDKGVVKSTSGS yxlc_bacsu Uncharacterized protein YxlC yxlC BSU38690 protein protein bacsu Bacillus subtilis MNKEKLSDHLKSEWKKIDQTANPSIPNQKELLHQLSQMKAEYRKKLLQEIILFVFCALMVVSAAILAFTQAPAVFIVLQVCVLAVLPILIAAEKKRHLGECEVKRG sigy_bacsu RNA polymerase sigma factor SigY sigY yxlB BSU38700 protein protein bacsu Bacillus subtilis MDTQEEQRLIQQAKEGNDEAFTALFHYHYSFLYKYLLKLSLHPDLSEELVQETFLKGYIHLRSFQGRSKFSTWLISIASRLYLDHQKKRKREWKRNQTVTEETIRKIKWDVSAKGAEWSETLDLFSKLDPKLRTPVLLRHYYGYTYAEIGVMLQIKEGTVKSRVHKGLQQIRKEWDDE yhdl_bacsu Probable anti-sigma-M factor YhdL yhdL BSU09510 protein protein bacsu Bacillus subtilis MMNEEFKKRFDQYKNGEMSDQEMTAFEEELEKLEVYQELIDSELEDDNDWDLSISPEKQKAILAYGKRKSYLRISVLAVISTLMILPLCTLGSYLYYGMGGKHSTGNEFMETAAVTVALTMPNVLVDTSGLKSQVKLFGMNTEFPLQKQIGTKTAAVGNERVEMFYNKVKAPAVNYYDLEVNKTGHYFTHPSNKSEQTTAKAEKTLSTLPEGTVSEVYLSYDRAYPTKDVYNKFKGYDVSFLWNAIETEKNTNKTASTEPLGYPGKDSKFLAALNTKGKSNGDQFINALKFMSKHEKWAQVISKRKDLNVDNRLDYVEKNGVNVYGSVVTGPTKEIQRMLKNKSVKSANVGEVELWNW sigm_bacsu ECF RNA polymerase sigma factor SigM sigM yhdM BSU09520 protein protein bacsu Bacillus subtilis MTIDEIYQMYMNDVYRFLLSMTKDKHLAEDLLQETFMRAYIHIHSYDHSKVKPWLFQVARNAFIDYVRKHKKEVTISDDLIGSLFQNAVQSPAHQVEIKEVLTGYMSELPDNYREALTLYYLKELNYKEASHIMNISEANFKSVLFRARQRLKALYNRGVNDE yhdk_bacsu Probable anti-sigma-M factor YhdK yhdK BSU09500 protein protein bacsu Bacillus subtilis MELVRIFKEHNVFGWISVGTAVLSLLLLNLAIISNVTFYSYQMLPFAMAAVPFGVVELFIKRGRTGPGLLGVILNLFVIICVYTIVSVDTNLQFGF sigv-yhrm 781528 n/a 781529 unspecified role unspecified role prey prey n-terminus ntphis 1'-phospho-L-histidine 1'-phospho-L-histidine histidine-N1'-phosphate (2S)-2-amino-3-(1-phosphono-1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1-phosphono-1H-imidazol-4-yl)propanoic acid ACT_SITE Tele-phosphohistidine intermediate MOD_RES Tele-phosphohistidine N(tau)-phosphohistidine N1-phosphonohistidine NE2-phosphonohistidine Ntau-phosphorylated L-histidine histidine-N(epsilon)-phosphate tele-phosphohistidine histidine-3-phosphate NtPHis Phospho Phosphorylation certain certain sequence position certain certain certain sequence position certain false n/a 781530 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4 sigw-ybbm 781528 n/a 781531 unspecified role unspecified role prey prey n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 781532 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4 sigx-ypun 781528 n/a 781533 unspecified role unspecified role prey prey n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 781534 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4 sigy-yxlc 781528 n/a 781535 unspecified role unspecified role prey prey n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 781536 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4 sigm-yhdl 781528 n/a 781537 unspecified role unspecified role prey prey n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 781538 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4 &5b yhdk-yhdl 781528 n/a 781539 unspecified role unspecified role bait bait n/a 781537 unspecified role unspecified role prey prey n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5B yhdk-yhdl-1 781528 n/a 781539 unspecified role unspecified role bait bait n/a 781537 unspecified role unspecified role prey prey c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5B yhdk-yhdl-2 781528 n/a 781537 unspecified role unspecified role prey prey n/a 781539 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5B yhdk-yhdl-3 781528 n/a 781537 unspecified role unspecified role bait bait c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 781539 unspecified role unspecified role prey prey n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5B