IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ bouquin-2003-1 The Arabidopsis lue1 mutant defines a katanin p60 ortholog involved in hormonal control of microtubule orientation during cell growth. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Bouquin T., Mattsson O., Naested H., Foster R., Mundy J. mundy@biobase.dk Arabidopsis mature leaf cDNA library J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation bouquin-2003-2 The Arabidopsis lue1 mutant defines a katanin p60 ortholog involved in hormonal control of microtubule orientation during cell growth. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bouquin T., Mattsson O., Naested H., Foster R., Mundy J. mundy@biobase.dk Arabidopsis mature leaf cDNA library J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation bouquin-2003-3 The Arabidopsis lue1 mutant defines a katanin p60 ortholog involved in hormonal control of microtubule orientation during cell growth. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bouquin T., Mattsson O., Naested H., Foster R., Mundy J. mundy@biobase.dk Arabidopsis mature leaf cDNA library J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation ktna1_arath Katanin p60 ATPase-containing subunit A1 AAA1 BOT1 ERH3 FRA2 FTR KSS KTN1 LUE1 p60 katanin Katanin-1 CAD ATPase Protein BOTERO 1 Protein ECTOPIC ROOT HAIR 3 Protein FAT ROOT Protein FRAGILE FIBER 2 F5I6.10 At1g80350 Katanin-like microtubule-severing protein protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVGSSNSLAGLQDHLKLAREYALEGSYDTSVIFFDGAIAQINKHLNTLDDPLARTKWMNVKKAIMEETEVVKQLDAERRAFKEAPTGRRAASPPINTKSSFVFQPLDEYPTSSGGGPMDDPDVWRPPTRDVTSRRPARAGQTGTRKSPQDGAWARGPTTRTGPASRGGRGGATSKSTAGARSSTAGKKGAASKSNKAESMNGDAEDGKSKRGLYEGPDEDLAAMLERDVLDSTPGVRWDDVAGLSEAKRLLEEAVVLPLWMPEYFQGIRRPWKGVLMFGPPGTGKTLLAKAVATECGTTFFNVSSATLASKWRGESERMVRCLFDLARAYAPSTIFIDEIDSLCNSRGGSGEHESSRRVKSELLVQVDGVSNTATNEDGSRKIVMVLAATNFPWDIDEALRRRLEKRIYIPLPDFESRKALININLRTVEVASDVNIEDVARRTEGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKDDISNDPVAMCDFEEAIRKVQPSVSSSDIEKHEKWLSEFGSA ktn82_arath Katanin p80 WD40 repeat-containing subunit B1 homolog KTN80.2 KTN80.2 DDB1 binding WD40 hypersensitive to ABA 3 DWA3 F11P17.7 At1g61210 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAKRGYKLQEFLAHSANVNCLSIGKKTSRLFITGGDDYKVNLWAIGKPTSLMSLCGHTSAVDSVAFDSAEVLVLAGASSGVIKLWDVEEAKMVRAFTGHRSNCSAVEFHPFGEFLASGSSDANLKIWDIRKKGCIQTYKGHSRGISTIRFTPDGRWVVSGGLDNVVKVWDLTAGKLLHEFKFHEGPIRSLDFHPLEFLLATGSADRTVKFWDLETFELIGSTRPEATGVRSIKFHPDGRTLFCGLDDSLKVYSWEPVVCHDGVDMGWSTLGDLCISEGKLLGCSYYQNSVGIWVSDISQIEPYGIGSADKKECVEKILSALDDQSFDRIKSTPRRSSSPDYETKEIKNIYIDSTGGNSAVAHKSGSLSTPATSTGQAGDNKSLVVHSVVPRDSDIGKDSSDSGKESITFSKTKPGMLLRPAYVRKTPTKFDETKKQSVAVGYLKKSGLDGEKKLDTETAFDSEMSGRNPYDADDSIIKSITNKFEQALLPESPTDEAKCMLLKPPRVQRSPSTKYNEARWATSTDSGALDSKKNGLESSRDMDLPTGLRDDRGSNPCEEDIENKSISSRSERVLSPEKAGDELKSLESPGGSKESKSVKVVRGVKVVSGRTRSLVERFERGEKITHSEDKAASATVVHSSNSVEEEPLTASVQTVSMMPTQVMPVKLDQATNSTTVDVPVLSTRRTKSTPVRVMPVVLGRDTSMATDTPPVTSTRPDRTSATNLTSDVSGVTSKRQTRTSPAPVMPMILNQTTKMKSDEPSITSTWPDRTSATDLTSDVSGVISSRQTRTSPAPVMPMKLNQKTKIKSDEPPITSTRPDRPSATNLTSDESPVTSTRQAKTSPAPVTPVILNQRQTTNMKSDEPPVISTRPLRTSSARVMPVILNQASTTYDERPLSSSRSARTSPARIMPMKLNQADNMPSYEPPVALTRSARNSPARVIPVKLNQATNVTADASHIRSRQRFSPTQTLATPAVFDQVTDMTLDETTKTQQSSDILTQKEEPQISGREDDGDIWEILMRTHSEVLNTLQSRLTKLQIVRHFWERSDIKGAIAALRKLSDHSVQADVINILTDKTEILTLDLFSQLAPVLTGLLGSKTERPVNVSLEMLLKLVAVFGTVIQSTVSARRVVGVDLHAEERLQICQSCSAELQKVQKILPLLTRRGGLIARKAQELNLVLQTP kn14a_arath Kinesin-like protein KIN-14A KIN14A GRIMP Geminivirus Rep-interacting motor protein Geminivirus replication protein-interacting protein KAC1 KCA1 KSN1 Kinesin CDKA-1-associated protein 1 Kinesin-like protein KCA1 Kinesin-like protein for actin-based chloroplast movement 1 TH65 F12B17.180 At5g10470 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADQRSKTNRWNWEVSGFEPRKSSSNASFAESTGHRTTGPLLRRNSISTPSLPPKQAIASKVNGLKEKVKLAKEDYLELRQEATDLQEYSNAKLDRVTRYLGVLAEKSRKLDQFVLETEARISPLINEKKRLFNDLLTAKGNIKVFCRARPLFEDEGPSVIEFPGDCTICVNTSDDTLSNPKKDFEFDRVYGPHVGQAALFSDVQPFVQSALDGSNVSILSYGQTNAGKTYTMEGSNHDRGLYARCFEELFDLANSDSTSTSRFSFSLSVFEIYNEQIRDLLSETQSNLPNINMDLHESVIELGQEKVDNPLEFLGVLKSAFLNRGNYKSKFNVTHLIVSIHIYYSNTITGENIYSKLSLVDLAGSEGLIMENDSGDHVTDLLHVMNSISALGDVLSSLTSGKDSIPYDNSILTRVLADSLGGSSKTLMIVNICPSVQTLSETISCLNYAARARNTVPSLGNRDTIKKWRDVASDARKELLEKERENQNLKQEVVGLKKALKDANDQCVLLYSEVQRAWKVSFTLQSDLKSENIMLVDKHRLEKEQNSQLRNQIAQFLQLDQEQKLQMQQQDSAIQNLQAKITDLESQVSEAVRSDTTRTGDALQSQDIFSPIPKAVEGTTDSSSVTKKLEEELKKRDALIERLHEENEKLFDRLTERSMAVSTQVLSPSLRASPNIQPANVNRGEGYSAEAVALPSTPNKNNGAITLVKSGTDLVKTTPAGEYLTAALNDFDPEEYEGLAAIADGANKLLMLVLAAVIKAGASREHEILAEIRDSVFSFIRKMEPRRVMDTMLVSRVRILYIRSLLARSPELQTIRVSPVECFLEKPNTGRSKSTSRGSSPGRSPVRYLDTQIHGFKVNIKAERRNKLASVVSRMRGLEQDAGRQQVTGVKLREMQDEAKSFAIGNKALAALFVHTPAGELQRQIRLWLAENFEFLSVTSDDVSGGNGGQLELLSTAIMDGWMAGLGAAVPPHTDALGQLLSEYAKRVYTSQMQHMKDIAGTLAAEEAEDAGQVSKLRSALESVDHKRRKILQQMKSDAALLNLEEGSSPIPNPSTAAEDSRLASLISLDGILKQVKEITRQASVHVLSKSKKKALLESLDELTERMPSLLDIDHPCAQREIATAHQLVETIPEQEDTNILEQSHDRRPSLESISSGETDVSQWNVLQFNTGSSAPFIIKCGGNNNSELVIKADARVQEPKGGEIVRVVPRPSVLVNMSLEEMKQMFVQLPEALSLLALARTADGTRARYSRLYKTLAMKVPSLKDLVSELE aaa1-at1g61210 772376 n/a 772379 unspecified role unspecified role bait bait n/a 772380 unspecified role unspecified role prey prey physical association physical association false false false Figure 6C Figure 6D aaa1-grimp 772376 n/a 772379 unspecified role unspecified role bait bait n/a 772381 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6C Figure 6D aaa1-grimp-1 772377 n/a 772381 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 772379 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Figure 6E aaa1-at1g61210-1 772377 n/a 772379 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 772380 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Figure 6E aaa1-grimp-2 772378 n/a 772379 unspecified role unspecified role bait bait n/a 772381 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6B aaa1-at1g61210-2 772378 n/a 772379 unspecified role unspecified role bait bait n/a 772380 unspecified role unspecified role prey prey physical association physical association false false false Figure 6B