IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nakamura-2001a-1 Physical interactions between ABA response loci of Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nakamura S., Lynch TJ., Finkelstein RR. All protein identifiers were derived from references provided in the text. finkelst@lifesci.ucsb.edu Plant J. (0960-7412) 2001 Only protein-protein interactions imex curation nakamura-2001a-2 Physical interactions between ABA response loci of Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 1 hybrid one hybrid yeast one-hybrid yeast one hybrid one-hybrid 1 hybrid yeast one-hybrid one-hybrid yeast one hybrid predetermined predetermined participant predetermined Nakamura S., Lynch TJ., Finkelstein RR. All protein identifiers were derived from references provided in the text. finkelst@lifesci.ucsb.edu Plant J. (0960-7412) 2001 Only protein-protein interactions imex curation abi5_arath Protein ABSCISIC ACID-INSENSITIVE 5 At2g36270 ABI5 BZIP39 DPBF1 GIA1 NEM1 Dc3 promoter-binding factor 1 Protein GROWTH-INSENSITIVITY TO ABA 1 bZIP transcription factor 39 F2H17.12 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVTRETKLTSEREVESSMAQARHNGGGGGENHPFTSLGRQSSIYSLTLDEFQHALCENGKNFGSMNMDEFLVSIWNAEENNNNQQQAAAAAGSHSVPANHNGFNNNNNNGGEGGVGVFSGGSRGNEDANNKRGIANESSLPRQGSLTLPAPLCRKTVDEVWSEIHRGGGSGNGGDSNGRSSSSNGQNNAQNGGETAARQPTFGEMTLEDFLVKAGVVREHPTNPKPNPNPNQNQNPSSVIPAAAQQQLYGVFQGTGDPSFPGQAMGVGDPSGYAKRTGGGGYQQAPPVQAGVCYGGGVGFGAGGQQMGMVGPLSPVSSDGLGHGQVDNIGGQYGVDMGGLRGRKRVVDGPVEKVVERRQRRMIKNRESAARSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLKSRAQPKLPKSNGRLRTLMRNPSCPL abi3_arath B3 domain-containing transcription factor ABI3 ABI3 MSD24.2 At3g24650 Protein ABSCISIC ACID-INSENSITIVE 3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKSLHVAANAGDLAEDCGILGGDADDTVLMDGIDEVGREIWLDDHGGDNNHVHGHQDDDLIVHHDPSIFYGDLPTLPDFPCMSSSSSSSTSPAPVNAIVSSASSSSAASSSTSSAASWAILRSDGEDPTPNQNQYASGNCDDSSGALQSTASMEIPLDSSQGFGCGEGGGDCIDMMETFGYMDLLDSNEFFDTSAIFSQDDDTQNPNLMDQTLERQEDQVVVPMMENNSGGDMQMMNSSLEQDDDLAAVFLEWLKNNKETVSAEDLRKVKIKKATIESAARRLGGGKEAMKQLLKLILEWVQTNHLQRRRTTTTTTNLSYQQSFQQDPFQNPNPNNNNLIPPSDQTCFSPSTWVPPPPQQQAFVSDPGFGYMPAPNYPPQPEFLPLLESPPSWPPPPQSGPMPHQQFPMPPTSQYNQFGDPTGFNGYNMNPYQYPYVPAGQMRDQRLLRLCSSATKEARKKRMARQRRFLSHHHRHNNNNNNNNNNQQNQTQIGETCAAVAPQLNPVATTATGGTWMYWPNVPAVPPQLPPVMETQLPTMDRAGSASAMPRQQVVPDRRQGWKPEKNLRFLLQKVLKQSDVGNLGRIVLPKKEAETHLPELEARDGISLAMEDIGTSRVWNMRYRFWPNNKSRMYLLENTGDFVKTNGLQEGDFIVIYSDVKCGKYLIRGVKVRQPSGQKPEAPPSSAATKRQNKSQRNINNNSPSANVVVASPTSQTVK em6-arath-dna Genomic DNA Em6 (Embryogenesis abundant gene Em6) dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress abi5-abi5 ABI5 Homodimer 739811 N/A 739813 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 739813 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 1b Figure 5 The authors commented "a weak ABI5 dimerization interaction was observed with an even shorter BD-ABI5 construct (including residues 262+/-442), the lack of dimerization by the aa 123+/-442 construct might reflect inaccessibility of the bZIP domain". abi5-abi3 ABI5 interacts with ABI3 739811 N/A 739814 unspecified role unspecified role prey prey N/A 739813 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 1b Figure 4 Figure 5 The authors carried out deletion analysis of the ABI3 protein and state " strong interactions were observed only when the B1 domain was present, although the B1 domain alone was not suffcient for strong interactions ". But the coordinates of this region are not provided. It was not possible to determine the coordinates of this region and this data was not curated. abi5-dna ABI5 interacts with Em6 gene promoter region 739812 N/A 739813 unspecified role unspecified role prey prey N/A 739815 unspecified role unspecified role bait bait physical association physical association false false false Figure 6