IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ albrecht-2001-1 The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Albrecht V., Ritz O., Linder S., Harter K., Kudla J. joerg.kudla@biologie.uni-ulm.de EMBO J. (0261-4189) 2001 Only protein-protein interactions imex curation albrecht-2001-2 The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Albrecht V., Ritz O., Linder S., Harter K., Kudla J. joerg.kudla@biologie.uni-ulm.de EMBO J. (0261-4189) 2001 Only protein-protein interactions imex curation albrecht-2001-3 The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Albrecht V., Ritz O., Linder S., Harter K., Kudla J. joerg.kudla@biologie.uni-ulm.de EMBO J. (0261-4189) 2001 Only protein-protein interactions imex curation cipk1_arath CBL-interacting serine/threonine-protein kinase 1 CIPK1 PKS13 SnRK3.16 MKP6.6 At3g17510 SOS2-like protein kinase PKS13 SNF1-related kinase 3.16 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVRRQEEEKKAEKGMRLGKYELGRTLGEGNFGKVKFAKDTVSGHSFAVKIIDKSRIADLNFSLQIKREIRTLKMLKHPHIVRLHEVLASKTKINMVMELVTGGELFDRIVSNGKLTETDGRKMFQQLIDGISYCHSKGVFHRDLKLENVLLDAKGHIKITDFGLSALPQHFRDDGLLHTTCGSPNYVAPEVLANRGYDGAASDIWSCGVILYVILTGCLPFDDRNLAVLYQKICKGDPPIPRWLSPGARTMIKRMLDPNPVTRITVVGIKASEWFKLEYIPSIPDDDDEEEVDTDDDAFSIQELGSEEGKGSDSPTIINAFQLIGMSSFLDLSGFFEQENVSERRIRFTSNSSAKDLLEKIETAVTEMGFSVQKKHAKLRVKQEERNQKGQVGLSVTAEVFEIKPSLNVVELRKSYGDSCLYRQLYERLLKDVGTSSPEQEIVT cnbl2_arath Calcineurin B-like protein 2 CBL2 SCABP1 At5g55990 MDA7.3 SOS3-like calcium-binding protein 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQCVDGIKHLCTSVLGCFDLDLYKQSGGLGDPELLARDTVFSVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIDDKIHFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLKDTVIEDIIDKTFEEADTKHDGKIDKEEWRSLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSQVEDT cnbl1_arath Calcineurin B-like protein 1 SOS3-like calcium-binding protein 5 CBL1 SCABP5 At4g17615 dl4845w FCAALL.122 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCFHSKAAKEFRGHEDPVKLASETAFSVSEVEALFELFKSISSSVVDDGLINKEEFQLALFKSRKRENIFANRIFDMFDVKRKGVIDFGDFVRSLNVFHPNASLEDKIDFTFRLYDMDCTGYIERQEVKQMLIALLCESEMKLADETIEIILDKTFEDADVNQDGKIDKLEWSDFVNKNPSLLKIMTLPYLRDITTTFPSFVFHSEVDEIAT cnbl3_arath Calcineurin B-like protein 3 SOS3-like calcium-binding protein 6 CBL3 SCABP6 At4g26570 T15N24.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQCIDGFKHVCSSFFRCFDIDIYKQSGGLGDPELLARETVFSVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIEDKIDFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLSDEIIESIIDKTFEEADTKHDGRIDKEEWRTLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSQVEDT cnbl4_arath Calcineurin B-like protein 4 Protein SALT OVERLY SENSITIVE 3 CBL4 SOS3 At5g24270 MOP9.8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCSVSKKKKKNAMRPPGYEDPELLASVTPFTVEEVEALYELFKKLSSSIIDDGLIHKEEFQLALFRNRNRRNLFADRIFDVFDVKRNGVIEFGEFVRSLGVFHPSAPVHEKVKFAFKLYDLRQTGFIEREELKEMVVALLHESELVLSEDMIEVMVDKAFVQADRKNDGKIDIDEWKDFVSLNPSLIKNMTLPYLKDINRTFPSFVSSCEEEEMELQNVSS cnbl5_arath Calcineurin B-like protein 5 SOS3-like calcium-binding protein 4 CBL5 SCABP4 At4g01420 F3D13.2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCVCSKQLEGRRQEDISLLASQTFFSEAEVEVLHGLFIKLTSCLSNDNLLTKEKFQFILIKNTKKRSLSAERIFGLFDMRNDGAIDFGEFVHTLNIFHPNSSPRDKAIFAFRLYDTRETGFIEPEEVKEMIIDVLEESELMLSESIIDSIVSKTFEEADWKKDGIIDLEEWENFVATYPLTLKNMTIPFLKDIPRIFPTFLR cnbl6_arath Calcineurin B-like protein 6 CBL6 SCABP2 dl4205c FCAALL.89 At4g16350 SOS3-like calcium-binding protein 2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMMQCLDGLKHLALILLTCCDADPPKVRQNPKDVARGTVFTVNEIEALYELFKSISKNGLIDKEQFQLVLFKMNTTRSLFADRVFDLFDTKNTGILDFEAFARSLSVFHPNAKFEDKIEFSFKLYDLNQQGYIKRQEVKQMVVRTLAESGMNLSDHVIESIIDKTFEEADTKLDGKIDKEEWRSLVLRHPSLLQNMSLQHLKDVTKTFPNFVFHTIVTDTPSELDG o82051_sorbi Non-specific serine/threonine protein kinase SNFL3 protein protein sorbi Sorghum bicolor (Sorghum) Sorghum Sorghum vulgare MDERRTILMDRYEIGRQLGQGNFAKVYFARNLTDGQSVAIKMIDKDKITRVGLIVQIKREISIMRLVKHPNVLQLFEVMASKSKIYFVLEYAKGGELFNKISKGKFSEDVARRYFHQLISAVDYCHSRGVYHRDLKPENLLLDENENLKVSDFGLSALAESKRHDGLLHTTCGTPAYVAPEVLSRRGYDGTKADIWSCGVILFVLVAGYLPFHDPNLIEMYRKISKAEYRCPRSFSGELKDLLFRMLDPDPSTRISISRIKRSTWYRKPIEVNSAKIKHDTTRDKVCNGEATTSNSIECSNSEETRGSSSLPNLNAFDIISLSTGFDLSNLFEEKYGRREERFTTRQPAGTVFAKLNELAERLKLKIKKKENGVLKLASPKEGIKGFLELDAEIFELAPSFLLVELKKTNGDTIEYQRLVKEEIRPALKDMVWAWQSDRHQQRNESVQGEQQLSSPLPPQQ cipk2_arath CBL-interacting serine/threonine-protein kinase 2 CIPK2 At5g07070 PKS16 SnRK3.2 SOS2-like protein kinase PKS16 SNF1-related kinase 3.2 T28J14.10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MENKPSVLTERYEVGRLLGQGTFAKVYFGRSNHTNESVAIKMIDKDKVMRVGLSQQIKREISVMRIAKHPNVVELYEVMATKSRIYFVIEYCKGGELFNKVAKGKLKEDVAWKYFYQLISAVDFCHSRGVYHRDIKPENLLLDDNDNLKVSDFGLSALADCKRQDGLLHTTCGTPAYVAPEVINRKGYEGTKADIWSCGVVLFVLLAGYLPFHDTNLMEMYRKIGKADFKCPSWFAPEVKRLLCKMLDPNHETRITIAKIKESSWFRKGLHLKQKKMEKMEKQQVREATNPMEAGGSGQNENGENHEPPRLATLNAFDIIALSTGFGLAGLFGDVYDKRESRFASQKPASEIISKLVEVAKCLKLKIRKQGAGLFKLERVKEGKNGILTMDAEIFQVTPTFHLVEVKKCNGDTMEYQKLVEEDLRPALADIVWVWQGEKEKEEQLLQDEQGEQEPS cipk7_arath CBL-interacting serine/threonine-protein kinase 7 CIPK7 PKS7 At3g23000 SnRK3.10 SR2 SRPK1 MXC7.3 SOS2-like protein kinase PKS7 SNF1-related kinase 3.10 Serine/threonine-protein kinase SR2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MESLPQPQNQSSPATTPAKILLGKYELGRRLGSGSFAKVHLARSIESDELVAVKIIEKKKTIESGMEPRIIREIDAMRRLRHHPNILKIHEVMATKSKIYLVMELASGGELFSKVLRRGRLPESTARRYFQQLASALRFSHQDGVAHRDVKPQNLLLDEQGNLKVSDFGLSALPEHLQNGLLHTACGTPAYTAPEVISRRGYDGAKADAWSCGVILFVLLVGDVPFDDSNIAAMYRKIHRRDYRFPSWISKQAKSIIYQMLDPNPVTRMSIETVMKTNWFKKSLETSEFHRNVFDSEVEMKSSVNSITAFDLISLSSGLDLSGLFEAKKKKERRFTAKVSGVEVEEKAKMIGEKLGYVVKKKMMKKEGEVKVVGLGRGRTVIVVEAVELTVDVVVVEVKVVEGEEDDSRWSDLITELEDIVLSWHNDIM cipk9_arath CBL-interacting serine/threonine-protein kinase 9 CIPK9 At1g01140 PKS6 SnRK3.12 F6F3.28 T25K16.13 SOS2-like protein kinase PKS6 SNF1-related kinase 3.12 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSGSRRKATPASRTRVGNYEMGRTLGEGSFAKVKYAKNTVTGDQAAIKILDREKVFRHKMVEQLKREISTMKLIKHPNVVEIIEVMASKTKIYIVLELVNGGELFDKIAQQGRLKEDEARRYFQQLINAVDYCHSRGVYHRDLKPENLILDANGVLKVSDFGLSAFSRQVREDGLLHTACGTPNYVAPEVLSDKGYDGAAADVWSCGVILFVLMAGYLPFDEPNLMTLYKRICKAEFSCPPWFSQGAKRVIKRILEPNPITRISIAELLEDEWFKKGYKPPSFDQDDEDITIDDVDAAFSNSKECLVTEKKEKPVSMNAFELISSSSEFSLENLFEKQAQLVKKETRFTSQRSASEIMSKMEETAKPLGFNVRKDNYKIKMKGDKSGRKGQLSVATEVFEVAPSLHVVELRKTGGDTLEFHKFYKNFSSGLKDVVWNTDAAAEEQKQ cipkb_arath CBL-interacting serine/threonine-protein kinase 11 SOS2-like protein kinase PKS5 SNF1-related kinase 3.22 SOS3-interacting protein 4 CIPK11 PKS5 SIP4 SnRK3.22 At2g30360 T9D9.17 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPEIEIAAGSGDNNDALFGKYELGKLLGCGAFAKVFHARDRRTGQSVAVKILNKKKLLTNPALANNIKREISIMRRLSHPNIVKLHEVMATKSKIFFAMEFVKGGELFNKISKHGRLSEDLSRRYFQQLISAVGYCHARGVYHRDLKPENLLIDENGNLKVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILSKKGYEGAKVDVWSCGIVLFVLVAGYLPFNDPNVMNMYKKIYKGEYRFPRWMSPDLKRFVSRLLDINPETRITIDEILKDPWFVRGGFKQIKFHDDEIEDQKVESSLEAVKSLNAFDLISYSSGLDLSGLFAGCSNSSGESERFLSEKSPEMLAEEVEGFAREENLRMKKKKEEEYGFEMEGQNGKFGIGICISRLNDLLVVVEARRRGGDGDCYKEMWNGKLRVQLIRVCDQTSSTNAAI cipke_arath CBL-interacting serine/threonine-protein kinase 14 CIPK14 At5g01820 PKS24 SnRK3.15 SR1 SOS2-like protein kinase PKS24 SNF1-related kinase 3.15 Serine/threonine-protein kinase SR1 T20L15.90 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVDSDPVEFPPENRRGQLFGKYEVGKLVGCGAFAKVYHGRSTATGQSVAIKVVSKQRLQKGGLNGNIQREIAIMHRLRHPSIVRLFEVLATKSKIFFVMEFAKGGELFAKVSKGRFCEDLSRRYFQQLISAVGYCHSRGIFHRDLKPENLLLDEKLDLKISDFGLSALTDQIRPDGLLHTLCGTPAYVAPEVLAKKGYDGAKIDIWSCGIILFVLNAGYLPFNDHNLMVMYRKIYKGEFRIPKWTSPDLRRLLTRLLDTNPQTRITIEEIIHDPWFKQGYDDRMSKFHLEDSDMKLPADETDSEMGARRMNAFDIISGSPGFNLSGLFGDARKYDRVERFVSAWTAERVVERLEEIVSAENLTVAKKETWGMKIEGQKGNFAMVVEINQLTDELVMIEVRKRQRAAASGRDLWTDTLRPFFVELVHESDQTDPEPTQVHTTS cipk4_arath CBL-interacting serine/threonine-protein kinase 4 CIPK4 PKS9 SnRK3.3 At4g14580 SOS2-like protein kinase PKS9 SNF1-related kinase 3.3 dl3330c FCAALL.259 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MESPYPKSPEKITGTVLLGKYELGRRLGSGSFAKVHVARSISTGELVAIKIIDKQKTIDSGMEPRIIREIEAMRRLHNHPNVLKIHEVMATKSKIYLVVEYAAGGELFTKLIRFGRLNESAARRYFQQLASALSFCHRDGIAHRDVKPQNLLLDKQGNLKVSDFGLSALPEHRSNNGLLHTACGTPAYTAPEVIAQRGYDGAKADAWSCGVFLFVLLAGYVPFDDANIVAMYRKIHKRDYRFPSWISKPARSIIYKLLDPNPETRMSIEAVMGTVWFQKSLEISEFQSSVFELDRFLEKEAKSSNAITAFDLISLSSGLDLSGLFERRKRKEKRFTARVSAERVVEKAGMIGEKLGFRVEKKEETKVVGLGKGRTAVVVEVVEFAEGLVVADVKVVVEGEEEEEEVESHWSELIVELEEIVLSWHN cipkc_arath CBL-interacting serine/threonine-protein kinase 12 SOS2-like protein kinase PKS8 SNF1-related kinase 3.9 F28A21.110 CIPK12 PKS8 At4g18700 SnRK3.9 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAEKITRETSLPKERSSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKEKVLKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEEVARKYFQQLISAVTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYRGEFRCPRWFSTELTRLLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVVDDDELESDSVESDRDSAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFDDDGEGSRFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFELTPSLVVVEVKKKGGDKTEYEDFCNNELKPKLQNLTADDVVAEPVAVSAVDETAIPNSPTISFLPSDTE cipkd_arath CBL-interacting serine/threonine-protein kinase 13 PKS10 SnRK3.7 SOS2-like protein kinase PKS10 SNF1-related kinase 3.7 F13P17.2 CIPK13 At2g34180 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAQVLSTPLAIPGPTPIQFMAGLLARIVTKNTNKETSTPESPRSPRTPQGSILMDKYEIGKLLGHGSFAKVYLARNIHSGEDVAIKVIDKEKIVKSGLAGHIKREISILRRVRHPYIVHLLEVMATKTKIYIVMEYVRGGELYNTVARGRLREGTARRYFQQLISSVAFCHSRGVYHRDLKLENLLLDDKGNVKVSDFGLSVVSEQLKQEGICQTFCGTPAYLAPEVLTRKGYEGAKADIWSCGVILFVLMAGYLPFDDKNILVMYTKIYKGQFKCPKWFSPELARLVTRMLDTNPDTRITIPEIMKHRWFKKGFKHVKFYIENDKLCREDDDNDDDDSSSLSSGRSSTASEGDAEFDIKRVDSMPRPASLNAFDILSFSDLSGLFEEGGQGARFVSAAPMTKIISKLEEIAKEVKFMVRKKDWSVRLEGCREGAKGPLTIRVEIFELTPSLVVVEVKKKGGNIEEYEEFCNKELRPQLEKLMHYQADEVEEVMCLPPEIEQ cipk6_arath CBL-interacting serine/threonine-protein kinase 6 SOS2-like protein kinase PKS4 SNF1-related kinase 3.14 SOS3-interacting protein 3 CIPK6 PKS4 At4g30960 SIP3 SnRK3.14 F6I18.130 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVGAKPVENGSDGGSSTGLLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRMVKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYFQQLISAVDFCHSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILKKGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTKLLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTEEDVDFLVHKSKEETETLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNKFDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALRPALKDIFWTSTPA cipk1-cbl2 CIPK1 interacts with CBL2 732729 N/A 732732 unspecified role unspecified role prey prey Q8RWC9:p.Ala320Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q8RWC9:p.Phe335Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q8RWC9:p.Ile324Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8RWC9:p.Ser327Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8RWC9:p.Leu332Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false Q8RWC9:p.Pro315Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false N/A 732733 unspecified role unspecified role bait bait physical association physical association false false false Figure 1A, Figure 1B, Figure 2A, Figure 2B There is a discrepancy between the text and figure legends in this paper with regard to the Q8RWC9 protein amino acid residues which are mutated. The text states that the mutation Ile322Asp effected binding between the proteins but in the Figure 2A the Ile residue in question is at position 323. Also the text describes the mutation at position 334 as Leu to a Asp conversion , in Figure 2A this is a Phe to Pro conversion. However in the UniProt record all residues which are mutated are +1 amino acid residue to the position identified in the text and the coordinates from the UniProt record are taken as the correct ones and presented here cipk1-cbl1-1 CIPK1 interacts with CBL1 732729 N/A 732734 unspecified role unspecified role bait bait N/A 732732 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 3, Figure 7A cipk1-cbl3 CIPK1 interacts with CBL3 732729 N/A 732735 unspecified role unspecified role bait bait N/A 732732 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 3 cipk1-cbl4 CIPK1 interacts with CBL4 732729 N/A 732736 unspecified role unspecified role bait bait N/A 732732 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 3 cipk1-cbl5 CIPK1 interacts with CBL5 732729 N/A 732737 unspecified role unspecified role bait bait N/A 732732 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 3 cipk1-cbl6 CIPK1 interacts with CBL6 732729 N/A 732732 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 732738 unspecified role unspecified role bait bait physical association physical association false false false Figure 3 snfl3-cbl2-3 Sorghum SNFL3 interacts with CBL2 732729 N/A 732739 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 732733 unspecified role unspecified role bait bait physical association physical association false false false Figure 5 cipk2-cbl1 CIPK2 interacts with CBL1 732729 n/a 732740 unspecified role unspecified role prey prey N/A 732734 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A atsrpk1-cbl1 AtSRPK1 (CIPK7) interacts with CBL1 732729 N/A 732734 unspecified role unspecified role bait bait N/A 732741 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A cipk9-cbl1-1 CIPK9 interacts with CBL1 732729 N/A 732734 unspecified role unspecified role bait bait N/A 732742 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A cipk11-cbl1 CIPK11 interacts with CBL1 732729 N/A 732743 unspecified role unspecified role prey prey N/A 732734 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A at5g01820-cbl1 At5g01820 (CIPK14) interacts with CBL1 732729 N/A 732734 unspecified role unspecified role bait bait N/A 732744 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A cipk2-cbl2 CIPK2 interacts with CBL2 732729 N/A 732733 unspecified role unspecified role bait bait n/a 732740 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A at4g14580-cbl2-1 At4g14580 (CIPK4) interacts with CBL2 732729 N/A 732745 unspecified role unspecified role prey prey N/A 732733 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A atsrpk1-cbl2-2 AtSRPK1 (CIPK7) interacts with CBL2 732729 N/A 732733 unspecified role unspecified role bait bait N/A 732741 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A cipk9-cbl2 CIPK9 interacts with CBL2 732729 N/A 732742 unspecified role unspecified role prey prey N/A 732733 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cipk11-cbl2-1 CIPK11 interacts with CBL2 732729 N/A 732743 unspecified role unspecified role prey prey N/A 732733 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A at4g18700-cbl2-2 At4g18700 (CIPK12) interacts with CBL2 732729 N/A 732746 unspecified role unspecified role prey prey N/A 732733 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cipk13-cbl2-3 CIPK13 interacts with CBL2 732729 N/A 732747 unspecified role unspecified role prey prey N/A 732733 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A at5g01820-cbl2 At5g01820 (CIPK14) interacts with CBL2 732729 N/A 732733 unspecified role unspecified role bait bait N/A 732744 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A at4g30960-cbl2-3 At4g30960 (CIPK6) interacts with CBL2 732729 N/A 732748 unspecified role unspecified role prey prey N/A 732733 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cipk2-cbl3 CIPK2 interacts with CBL3 732729 n/a 732740 unspecified role unspecified role prey prey N/A 732735 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A at4g14580-cbl3 At4g14580 (CIPK4) interacts with CBL3 732729 N/A 732745 unspecified role unspecified role prey prey N/A 732735 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A at4g30960-cbl3-1 At4g30960 (CIPK6) interacts with CBL3 732729 N/A 732748 unspecified role unspecified role prey prey N/A 732735 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A atsrpk1-cbl3-2 AtSRPK1 (CIPK7) interacts with CBL3 732729 N/A 732741 unspecified role unspecified role prey prey N/A 732735 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cipk9-cbl3-3 CIPK9 interacts with CBL3 732729 N/A 732735 unspecified role unspecified role bait bait N/A 732742 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A cipk11-cbl3 CIPK11 interacts with CBL3 732729 N/A 732735 unspecified role unspecified role bait bait N/A 732743 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A at4g18700-cbl3-1 At4g18700 (CIPK12) interacts with CBL3 732729 N/A 732746 unspecified role unspecified role prey prey N/A 732735 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cipk13-cbl3-2 CIPK13 interacts with CBL3 732729 N/A 732747 unspecified role unspecified role prey prey N/A 732735 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A at5g01820-cbl3-3 At5g01820 (CIPK14) interacts with CBL3 732729 N/A 732744 unspecified role unspecified role prey prey N/A 732735 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cipk2-cbl4 CIPK2 interacts with CBL4 732729 N/A 732736 unspecified role unspecified role bait bait n/a 732740 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A atsrpk1-cbl4-1 AtSRPK1 (CIPK7) interacts with CBL4 732729 N/A 732741 unspecified role unspecified role prey prey N/A 732736 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cip9-cbl4 CIPK9 interacts with CBL4 732729 N/A 732742 unspecified role unspecified role prey prey N/A 732736 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A at5g01820-cbl4-1 At5g01820 (CIPK14) interacts with CBL4 732729 N/A 732744 unspecified role unspecified role prey prey N/A 732736 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cipk2-cbl5-1 CIPK2 interacts with CBL5 732729 N/A 732737 unspecified role unspecified role bait bait n/a 732740 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A atsrpk1-cbl5-2 AtSRPK1 (CIPK7) interacts with CBL5 732729 N/A 732741 unspecified role unspecified role prey prey N/A 732737 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cipk11-cbl5-3 CIPK11 interacts with CBL5 732729 N/A 732743 unspecified role unspecified role prey prey N/A 732737 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A at5g01820-cbl5-4 At5g01820 (CIPK14) interacts with CBL5 732729 N/A 732744 unspecified role unspecified role prey prey N/A 732737 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A atsrpk1-cbl6-1 AtSRPK1 (CIPK7) interacts with CBL6 732729 N/A 732741 unspecified role unspecified role prey prey N/A 732738 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cipk11-cbl6-2 CIPK11 interacts with CBL6 732729 N/A 732738 unspecified role unspecified role bait bait N/A 732743 unspecified role unspecified role prey prey physical association physical association false false false Figure 7A at5g01820-cbl6-3 At5g01820 (CIPK14) interacts with CBL6 732729 N/A 732744 unspecified role unspecified role prey prey N/A 732738 unspecified role unspecified role bait bait physical association physical association false false false Figure 7A cipk1-cbl2-1 CIPK1 interacts with CBL2 732730 N/A 732733 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 732732 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Figure 1C, Figure 7B cipk13-cbl2 CIPK13 interacts with CBL2 732730 N/A 732747 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 732733 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Figure 7B at5g01820-cbl2-1 At5g01820 (CIPK14) interacts with CBL2 732730 N/A 732744 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 732733 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Figure 7B cipk1-cbl2-2 CIPK1 interacts with CBL2 732731 N/A 732732 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false Q8RWC9:p.Phe335Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false N/A 732733 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Figure 2C In the UniProt record all residues for protein Q8RWC9 which are mutated are +1 amino acid residue to the position identified in the text and the coordinates from the UniProt record are taken as the correct ones and presented here