IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ woitach-1998-2 A retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. rat-rle_phi13 Rattus norvegicus RLE (rat liver epithelial) sensitive to TGF-beta phi13 cell line rle_phi13 RLE (rat epithelial liver) phi 13 sensitive to TGF-beta non-transformed cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Woitach JT., Zhang M., Niu CH., Thorgeirsson SS. Cancer - Interactions investigated in the context of cancer The species of the pRb construct used was ascertained as the plasmid pASRb2 encodes human pRb (Huntley et al., 1998 Plant Mol Biol 37, 155-169). snorri_thorgeirsson@nih.gov Nat. Genet. (1061-4036) 1998 Only protein-protein interactions imex curation woitach-1998-1 A retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Woitach JT., Zhang M., Niu CH., Thorgeirsson SS. Cancer - Interactions investigated in the context of cancer The species of the pRb construct used was ascertained as the plasmid pASRb2 encodes human pRb (Huntley et al., 1998 Plant Mol Biol 37, 155-169). snorri_thorgeirsson@nih.gov Nat. Genet. (1061-4036) 1998 Only protein-protein interactions imex curation woitach-1998-3 A retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. rat-b5t Rattus norvegicus RLE (rat liver epithelial) (not sensitive to TGF-beta) B5T cell line b5t RLE (rat epithelial liver) B5T (not sensitive to TGF-beta) cell line These cells have a higher level of BOG than the RLE phi13 cell line. B5T cells have higher levels of BOG than RLE phi13 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Woitach JT., Zhang M., Niu CH., Thorgeirsson SS. Cancer - Interactions investigated in the context of cancer The species of the pRb construct used was ascertained as the plasmid pASRb2 encodes human pRb (Huntley et al., 1998 Plant Mol Biol 37, 155-169). snorri_thorgeirsson@nih.gov Nat. Genet. (1061-4036) 1998 Only protein-protein interactions imex curation rbbp9_rat Serine hydrolase RBBP9 Rbbp9 Bog B5T-overexpressed gene protein Retinoblastoma-binding protein 9 protein protein rat Rattus norvegicus (Rat) Rat MASPTKAVIVPGNGGGDVATHGWYGWVRKGLEQIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHQVYALILVSAYTSDLGDENERASGYFSRPWQWEKIKANCPHIIQFGSTDDPFLPWKEQQEVADRLDAKLYKFTDRGHFQNTEFHELIRVVKSMLTPAL rb_rat Retinoblastoma-associated protein pRb pp105 Rb1 Rb-1 protein protein rat Rattus norvegicus (Rat) Rat MPPKAPRRTAAAEPPPPPPPPPEDDPAQDSDPEELPLIRLEFEKIEEPEFIALCQKLKVPDHVRERAWLTWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTELQKSIETSVYKFFDLLKEIDTSTKVDNAVSRLLKKYNVLCALYSKLERTCGLIYLTQPSSGLSTEINSMLVLKVSWITFLLAKGEVVQMEDDLVISFQLMLCVLDYFIKLSPPALLREPYKTAATPINGSPRTPRRGQNRSARIAKQLESDTRTIEVLCKEHECNVDEVKNVYFKNFIPFISSLGIVSSNGLPELESLSKRYEEVYLKSKDLDARLFLDHDKTLQTDTIDSFETERTPRKSNPDEEANMVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFSNCTVNPKENILKRVKDVGHIFKEKFASAVGQGCIDIGAQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSMLQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKVEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGPDHLESACSLSLPLQSNHTAADMYLSPIRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLSLFYKKVYRLAYLRLNTLCARLLSDHPELEHIIWTLFQHTLENEYELMKDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGSDEADGSKHLPAESKFQQKLAEMTSTRTRMQKQKLNDSMEISNKEEK e2f1_rat Transcription factor E2F1 E2f1 protein protein rat Rattus norvegicus (Rat) Rat MAVAPAGGQHAPALEALLGAGALRLLDSSQIVIISTAPDVGAPQVPTGPAAPPAGPRDPDVLLFATPQAPRPAPSAPRPALGRPPVKRRLDLETDHQYLAGSSGPFRGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSRTMVGIGQRLEGLTQDLQQLQESEQQLDHLMHICTTQLQLLSEDSDIQRLAYVTCQDLRSIADPAEQMVIVIKAPPETQLQAVDSAETFQISLKSKQGPIDVFLCPEESAEGISPGRTSYQETSGEDRNADSGTAGPPPSPPSTSPTLDPSQSLLGLEQEAVLPRIGNLRAPMEEDRLSPLVAADSLLEHVKEDFSGLLPGEFISLSPPHEAVDYHFGLEEGEGIRDLFDCDFGDLTPLDF rbl2_rat Retinoblastoma-like protein 2 Rbl2 Retinoblastoma-related protein 2 130 kDa retinoblastoma-associated protein pRb2 PPAR-alpha-interacting complex protein 128 protein protein rat Rattus norvegicus (Rat) Rat MASGGNQSSPPPPAAAASSEEEEEDGDTADRAQPAGSPSHQIQQRFEELCSRLNMDEAARAEAWSSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTAEGNYVSLTRILRCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTTSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGVSEDGHPRDSHPSSDPPCVIEKLCSLHDGLVLEAKGIKQHFWKPYIRKLFEKKLLRGKEENLTGFLEPGNFAESFKAVNKAYEEYVLATGSLDERIFLGEDAEEEVGTFSRCVSAASGTESAERTQMRDILQQHLDKSKTLRVCNPLTGVRYVQENSPCVTPVSTATHSLNRLHTMLAGLRNAPSEKLEQILRSCSRDPTRAIADRLREMYEIYSQHFQPDENVSNCAKEMANKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGVLEQDAFHKSLLACCLEVVAFSYKPPGNFPFIAEIFDVPHYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKTKSPLWDRIRDNENRVPTCEEVTPPHNLERTDEIYIAGSPLTPRRVGEVRTDAGGLGRSVTSPTTLYDRYSSPTVSTTRRRLFESDSPSEGSTAGRIPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGPVAIQQISPGGQQQNQGQPLTSSSIRPRKTSSLSLFFRKVYYLAGVRLRDLCTKLDISDELRKKIWTCFEFSIVQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRRNSGSCENRSHQNSPTELNTDRASRDSSPVMRSNSTLPVPQPSSAPPTPTRLTGANSDIEEEERGDLIQFYNNIYRKQIQTFAMKYSQANSQMDTPPLSPYPFVRTGSPRRVQLSQSHPIYISPHKNEAMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLDDGSESPAKRICPENHSALLRRLQDVANDRGSH d3zs62_rat Rbl1 protein protein rat Rattus norvegicus (Rat) Rat MFEDEPHAEGAAAVAAAREALQALCQELNLDEGSAAEALDDFTAIRGNYSLEGEVIHWLACSLYVACRKSIIPTVGKGVMEGNCVSLTRILRSAKLSLIQFFSKMKKWMDMSNLPQEFRERIERLERNFEVSTVIFKKFEPIFLDIFQNPYEELPKLPRSRKQRRIPCSVKDLFNFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLIFANAIMCPNRRDLLNPSFKGLPSDFHAVNFKAAEEPPCIIAVLCDLHDGLLVEAKGIKEHYFKPYISKLFDKKILKGECLLDLSSFTDNSKAVNKEYEEYVLTVGDFDERIFLGADAEEEIGTPRKFAADTQFGKLTSQASVDCNLQQHFEKKRSFAPSTPLTGRRYLQEKEAVTTPVASATQSVSRLQSIVAGLKSAPSEQLLTIFESCMRNPMGNIVKIVKGIGETFCQHYTQSTDKQPGSHIDFAVNRLKLAEILYYKILETIMVQETRRLHGMDMSVLLEQDIFHRSLLACCLEIVLFAYSSPRTFPWIIDVLGLQPFYFYKVIEVVIRSEEGLSRDMVKHLNSIEEQILESLAWTNNSALWEALRASANKVPSCEEVIFPNNFEIGNGGSVQGHLPMMPMSPIIHPRVKEVRTDSGSLRKDMQPLSPISVHERYSSPAAGSAKRRLFGDDPPKETLMEKIMAEGTQLKIAPSSVTAESLSISPGQALLTMATTTVTGTTGRKVTVPLHGIANDAGEITLVPISMNTTQDSTAESLVSLTAQSLIGASPKQTHLTKTQDAPLTGISKPKRTGSLALFYRKVYHLASVRLRDLCLKLDVSNELRRKIWTCFEFTLVHCPDLMKDRHLDQLLLCAFYIMAKVTKEERTFQEIMKSYRNQPQANSHVYRSVLLKSIPGEVVAYNGDYEMTDGDIEDATKTPNCSSEPVKEERGDLIKFYNAIYVGRVKSFALKYDLSNQDHIMDAPPLSPFPHIKQQPGSPRRISQQHSIYVSPHKNASGLTPRSALLYKFNGSPSKSRKVLRNMARQGSRKAKKRVIAISGDAESPAKRLCQENDDVLLKRLQDVVSERANH The sequence has been withdrawn from uniprot. rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET rb1-rbbp9-1 pRb coimmunoprecipitates BOG in RLE phi13 cell extracts 596436 N/A 596439 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O88350:p.Leu63Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false N/A 596440 unspecified role unspecified role bait bait physical association physical association false false false 2 C The disruption of the interaction by the mutation leu63glu in Bog indicates that Bog interacts with Rb through the LXCXE sequence. rbbp9-rb1 pRb coimmunoprecipitates with BOG in RLE phi13 cell extracts 596436 N/A 596440 unspecified role unspecified role prey prey N/A 596439 unspecified role unspecified role bait bait physical association physical association false false false 2 B, D rb1-e2f1-1 E2F1 coimmunoprecipitates with pRb in RLE phi13 cell extracts 596436 N/A 596441 unspecified role unspecified role prey prey N/A 596440 unspecified role unspecified role bait bait physical association physical association false false false 2 B, C The authors state that in the B5T cells the amount of E2F1 in complex with pRb is dramatically reduced as compared to phi13 cells due to higher levels of BOG, which displaces E2F1 from complex with pRb. Incubation with Bog-MBP disrupts this interaction, but if Bog is mutated in the Rb-binding site it does not. e2f1-rb1 E2F1 coimmunoprecipitates pRb in RLE phi13 cell extracts 596436 N/A 596441 unspecified role unspecified role bait bait N/A 596440 unspecified role unspecified role prey prey physical association physical association false false false 2 B, C The authors state that in the B5T cells the amount of E2F1 in complex with pRb is dramatically reduced as compared to phi13 cells due to higher levels of BOG, which displaces E2F1 from complex with pRb. Incubation with Bog-MBP disrupts this interaction, but if Bog is mutated in the Rb-binding site it does not. rbbp9-rbl2 p130 coimmunoprecipitates with BOG in RLE phi13 cell extracts 596436 N/A 596442 unspecified role unspecified role prey prey N/A 596439 unspecified role unspecified role bait bait physical association physical association false false false 2 D rbl1_rat_prot-rbbp9 Bog coimmunoprecipitates p107 in RLE phi13 cell extracts 596436 N/A 596443 unspecified role unspecified role prey prey N/A 596439 unspecified role unspecified role bait bait physical association physical association false false false 2 D rb1-rbbp9 Human pRb interacts with rat BOG in a yeast two-hybrid assay 596437 N/A 596444 unspecified role unspecified role bait bait N/A 596439 unspecified role unspecified role prey prey physical association physical association false false false 2 A rb1-tala Human pRb interacts with SV40 large T antigen in a yeast two-hybrid assay 596437 N/A 596445 unspecified role unspecified role prey prey N/A 596444 unspecified role unspecified role bait bait physical association physical association false false false 2 A rb1-rbbp9-2 pRb coimmunoprecipitates BOG in B5T cell extracts 596438 N/A 596439 unspecified role unspecified role prey prey N/A 596440 unspecified role unspecified role bait bait physical association physical association false false false 2 B rbbp9-rb1-1 pRb coimmunoprecipitates with BOG in B5T cell extracts 596438 N/A 596439 unspecified role unspecified role bait bait N/A 596440 unspecified role unspecified role prey prey physical association physical association false false false 2 B