IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ reinders-1997-1 Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Reinders A., Burckert N., Hohmann S., Thevelein JM., Boller T., Wiemken A., De Virgilio C. devir-gilioc@ubaclu.unibas.ch Mol. Microbiol. (0950-382X) 1997 Only protein-protein interactions imex curation tps1_yeast Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit GGS1 GLC6 TSS1 TPS1 BYP1 CIF1 FDP1 YBR126C YBR0922 Trehalose-6-phosphate synthase UDP-glucose-glucosephosphate glucosyltransferase Trehalose synthase complex catalytic subunit TPS1 General glucose sensor subunit 1 Glycogen metabolism control protein GLC6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTTDNAKAQLTSSSGGNIIVVSNRLPVTITKNSSTGQYEYAMSSGGLVTALEGLKKTYTFKWFGWPGLEIPDDEKDQVRKDLLEKFNAVPIFLSDEIADLHYNGFSNSILWPLFHYHPGEINFDENAWLAYNEANQTFTNEIAKTMNHNDLIWVHDYHLMLVPEMLRVKIHEKQLQNVKVGWFLHTPFPSSEIYRILPVRQEILKGVLSCDLVGFHTYDYARHFLSSVQRVLNVNTLPNGVEYQGRFVNVGAFPIGIDVDKFTDGLKKESVQKRIQQLKETFKGCKIIVGVDRLDYIKGVPQKLHAMEVFLNEHPEWRGKVVLVQVAVPSRGDVEEYQYLRSVVNELVGRINGQFGTVEFVPIHFMHKSIPFEELISLYAVSDVCLVSSTRDGMNLVSYEYIACQEEKKGSLILSEFTGAAQSLNGAIIVNPWNTDDLSDAINEALTLPDVKKEVNWEKLYKYISKYTSAFWGENFVHELYSTSSSSTSSSATKN 47325A0FA3554E3D tps2_yeast Trehalose-phosphatase TPS2 PFK3 YDR074W YD8554.07 Trehalose-6-phosphate phosphatase Trehalose synthase complex catalytic subunit TPS2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTTTAQDNSPKKRQRIINCVTQLPYKIQLGESNDDWKISATTGNSALFSSLEYLQFDSTEYEQHVVGWTGEITRTERNLFTREAKEKPQDLDDDPLYLTKEQINGLTTTLQDHMKSDKEAKTDTTQTAPVTNNVHPVWLLRKNQSRWRNYAEKVIWPTFHYILNPSNEGEQEKNWWYDYVKFNEAYAQKIGEVYRKGDIIWIHDYYLLLLPQLLRMKFNDESIIIGYFHHAPWPSNEYFRCLPRRKQILDGLVGANRICFQNESFSRHFVSSCKRLLDATAKKSKNSSNSDQYQVSVYGGDVLVDSLPIGVNTTQILKDAFTKDIDSKVLSIKQAYQNKKIIIGRDRLDSVRGVVQKLRAFETFLAMYPEWRDQVVLIQVSSPTANRNSPQTIRLEQQVNELVNSINSEYGNLNFSPVQHYYMRIPKDVYLSLLRVADLCLITSVRDGMNTTALEYVTVKSHMSNFLCYGNPLILSEFSGSSNVLKDAIVVNPWDSVAVAKSINMALKLDKEEKSNLESKLWKEVPTIQDWTNKFLSSLKEQASSNDDMERKMTPALNRPVLLENYKQAKRRLFLFDYDGTLTPIVKDPAAAIPSARLYTILQKLCADPHNQIWIISGRDQKFLNKWLGGKLPQLGLSAEHGCFMKDVSCQDWVNLTEKVDMSWQVRVNEVMEEFTTRTPGSFIERKKVALTWHYRRTVPELGEFHAKELKEKLLSFTDDFDLEVMDGKANIEVRPRFVNKGEIVKRLVWHQHGKPQDMLKGISEKLPKDEMPDFVLCLGDDFTDEDMFRQLNTIETCWKEKYPDQKNQWGNYGFYPVTVGSASKKTVAKAHLTDPQQVLETLGLLVGDVSLFQSAGTVDLDSRGHVKNSESSLKSKLASKAYVMKRSASYTGAKV E349672CCCDA9349 tps3_yeast Trehalose synthase complex regulatory subunit TPS3 TPS3 YMR261C YM8156.03C Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 115 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MTIIVASLFLPYTPQFEADVTNSDTAKLVESSMIKVDCNNQELSNNKQERSSSVTSASSHYIGLPQEAQINGEPLQRANVGSPATGVNYHNEMEMLSSEQFLEELTANATHAANSGIPPANNPVSSGSTAQRPSVEEFFSAPSARVCSPSQEASASSISASRSSAHHNDLSSSLMKNPNLSFDSHPPRVRSSSKSAVITPVSKSVPDVDPAVVDVAKVREEFQQQASLPSMKRVSGSTAGDSSIASSSSNLRYSQQFQDNFIEDTDSEDDIDSDLETDATKKYNVPKFGGYSNNAKLRASLMRNSYELFKHLPWTIVDSDKGNGSLKNAVNIAVAEKTVKEPVSWVGTMGIPTDELPHEVCHKISKKLEQDFSSFPVVTDDITFKGAYKNYAKQILWPTLHYQIPDNPNSKAFEDHSWDYYQKVNQKFSDRIVSVYKPGDTIWIHDYHLMLVPQMVREKLPKAKIGFFLHVSFPSSEVFRCLANRERILEGIIGANFVGFQTKEYKRHFLQTCNRLLAADVSNDEVKYHCNIVSVMYAPIGIDYYHLTSQLRNGSVLEWRQLIKERWRNKKLIVCRDQFDRIRGLQKKMLAYERFLIENPEYIEKVVLIQICIGKSSDPEYERQIMVVVDRINSLSSNISISQPVVFLHQDLDFAQYLALNCEADVFLVDALREGMNLTCHEFIVSSFEKNAPLLLSEFTGSSSVLKEGAILINPWDINHVAQSIKRSLEMSPEEKRRRWKKLFKSVIEHDSDNWITKCFEYINNAWESNQETSTVFNLAPEKFCADYKASKKHLFIFKISEPPTSRMLSLLSELSSNNIVYVLSSFTKNTFESLYNGVLNIGLIAENGAYVRVNGSWYNIVEELDWMKEVAKIFDEKVERLPGSYYKIADSMIRFHTENADDQDRVPTVIGEAITHINTLFDDRDIHAYVHKDIVFVQQTGLALAAAEFLMKFYNSGVSPTDNSRISLSRTSSSMSVGNNKKHFQNQVDFVCVSGSTSPIIEPLFKLVKQEVEKNNLKFGYTILYGSSRSTYAKEHINGVNELFTILHDLTAA tsl1_yeast Trehalose synthase complex regulatory subunit TSL1 TSL1 YML100W Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 123 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MALIVASLFLPYQPQFELDTSLPENSQVDSSLVNIQAMANDQQQQRALSNNISQESLVAPAPEQGVPPAISRSATRSPSAFNRASSTTNTATLDDLVSSDIFMENLTANATTSHTPTSKTMLKPRKNGSVERFFSPSSNIPTDRIASPIQHEHDSGSRIASPIQQQQQDPTTNLLKNVNKSLLVHSLLNNTSQTSLEGPNNHIVTPKSRAGNRPTSAATSLVNRTKQGSASSGSSGSSAPPSIKRITPHLTASAAKQRPLLAKQPSNLKYSELADISSSETSSQHNESDPDDLTTAPDEEYVSDLEMDDAKQDYKVPKFGGYSNKSKLKKYALLRSSQELFSRLPWSIVPSIKGNGAMKNAINTAVLENIIPHRHVKWVGTVGIPTDEIPENILANISDSLKDKYDSYPVLTDDDTFKAAYKNYCKQILWPTLHYQIPDNPNSKAFEDHSWKFYRNLNQRFADAIVKIYKKGDTIWIHDYHLMLVPQMVRDVLPFAKIGFTLHVSFPSSEVFRCLAQREKILEGLTGADFVGFQTREYARHFLQTSNRLLMADVVHDEELKYNGRVVSVRFTPVGIDAFDLQSQLKDGSVMQWRQLIRERWQGKKLIVCRDQFDRIRGIHKKLLAYEKFLVENPEYVEKSTLIQICIGSSKDVELERQIMIVVDRINSLSTNISISQPVVFLHQDLDFSQYLALSSEADLFVVSSLREGMNLTCHEFIVCSEDKNAPLLLSEFTGSASLLNDGAIIINPWDTKNFSQAILKGLEMPFDKRRPQWKKLMKDIINNDSTNWIKTSLQDIHISWQFNQEGSKIFKLNTKTLMEDYQSSKKRMFVFNIAEPPSSRMISILNDMTSKGNIVYIMNSFPKPILENLYSRVQNIGLIAENGAYVSLNGVWYNIVDQVDWRNDVAKILEDKVERLPGSYYKINESMIKFHTENAEDQDRVASVIGDAITHINTVFDHRGIHAYVYKNVVSVQQVGLSLSAAQFLFRFYNSASDPLDTSSGQITNIQTPSQQNPSDQEQQPPASPTVSMNHIDFACVSGSSSPVLEPLFKLVNDEASEGQVKAGHAIVYGDATSTYAKEHVNGLNELFTIISRIIED EA0A8A86AF178F04 tps1-tps1-1 Interaction between Tps1 and Tps1 identified by yeast two-hybrid 606771 N/A 606772 unspecified role unspecified role prey prey N/A 606772 unspecified role unspecified role bait bait physical association physical association false false false beta-galactosidase activity = 548 miller units Table 1 tps1-tps2-1 Interaction between Tps1 and Tps2 identified by yeast two-hybrid 606771 N/A 606773 unspecified role unspecified role prey prey N/A 606772 unspecified role unspecified role bait bait physical association physical association false false false beta-galactosidase activity = 208 miller units Table 1 tps1-tps3-1 Interaction between Tps1 and Tps3 identified by yeast two-hybrid 606771 N/A 606774 unspecified role unspecified role prey prey N/A 606772 unspecified role unspecified role bait bait physical association physical association false false false beta-galactosidase activity = 708 miller units Table 1 tps1-tsl1-1 Interaction between Tps1 and Tsl1 identified by yeast two-hybrid 606771 N/A 606772 unspecified role unspecified role bait bait N/A 606775 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false beta-galactosidase activity = 53 miller units Table 1 tps1-tps2-2 Interaction between Tps1 and Tps2 identified by yeast two-hybrid 606771 N/A 606772 unspecified role unspecified role prey prey N/A 606773 unspecified role unspecified role bait bait physical association physical association false false false beta-galactosidase activity = 150 miller units Table 1 tps2-tps2-1 Interaction between Tps2 and Tps2 identified by yeast two-hybrid 606771 N/A 606773 unspecified role unspecified role prey prey N/A 606773 unspecified role unspecified role bait bait physical association physical association false false false beta-galactosidase activity = 528 miller units Table 1 tps2-tps3-1 Interaction between Tps2 and Tps3 identified by yeast two-hybrid 606771 N/A 606773 unspecified role unspecified role bait bait N/A 606774 unspecified role unspecified role prey prey physical association physical association false false false beta-galactosidase activity = 239 miller units Table 1 tps2-tsl1-1 Interaction between Tps2 and Tsl1 identified by yeast two-hybrid 606771 N/A 606773 unspecified role unspecified role bait bait N/A 606775 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false beta-galactosidase activity = 346 miller units Table 1 tps1-tps3-2 Interaction between Tps1 and Tps3 identified by yeast two-hybrid 606771 N/A 606774 unspecified role unspecified role bait bait N/A 606772 unspecified role unspecified role prey prey physical association physical association false false false beta-galactosidase activity = 696 miller units Table 1 tps2-tps3-2 Interaction between Tps2 and Tps3 identified by yeast two-hybrid 606771 N/A 606774 unspecified role unspecified role bait bait N/A 606773 unspecified role unspecified role prey prey physical association physical association false false false beta-galactosidase activity = 411 miller units Table 1 tps3-tps3-1 Interaction between Tps3 and Tps3 identified by yeast two-hybrid 606771 N/A 606774 unspecified role unspecified role bait bait N/A 606774 unspecified role unspecified role prey prey physical association physical association false false false beta-galactosidase activity = 425 miller units Table 1 tps1-tsl1-2 Interaction between Tps1 and Tsl1 identified by yeast two-hybrid 606771 N/A 606775 unspecified role unspecified role bait bait N/A 606772 unspecified role unspecified role prey prey physical association physical association false false false beta-galactosidase activity = 49 miller units Table 1