IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ koch-1993-2 A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa partial id prot seq partial identification of protein sequence partial id prot seq Koch C., Moll T., Neuberg M., Ahorn H., Nasmyth K. Column purified on a heparin agarose column and eluted with an ammonium sulfate gradient. Fractions containing the peak of MBF were further purified by DNA affinity chromatography with MCB elements from the TMPl promoter. Swi6 identified by comigration on a gel with a previously purified protein sample, Mbp1 was identified by peptide mapping. Science (0036-8075) 1993 koch-1993-1 A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom partial id prot seq partial identification of protein sequence partial id prot seq Koch C., Moll T., Neuberg M., Ahorn H., Nasmyth K. Column purified on a heparin agarose column and eluted with an ammonium sulfate gradient. Fractions containing the peak of MBF were further purified by DNA affinity chromatography with MCB elements from the TMPl promoter. Swi6 identified by comigration on a gel with a previously purified protein sample, Mbp1 was identified by peptide mapping. Science (0036-8075) 1993 mbp1_yeast Transcription factor MBP1 YDL056W MBP1 MBF subunit p120 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNQIYSARYSGVDVYEFIHSTGSIMKRKKDDWVNATHILKAANFAKAKRTRILEKEVLKETHEKVQGGFGKYQGTWVPLNIAKQLAEKFSVYDQLKPLFDFTQTDGSASPPPAPKHHHASKVDRKKAIRSASTSAIMETKRNNKKAEENQFQSSKILGNPTAAPRKRGRPVGSTRGSRRKLGVNLQRSQSDMGFPRPAIPNSSISTTQLPSIRSTMGPQSPTLGILEEERHDSRQQQPQQNNSAQFKEIDLEDGLSSDVEPSQQLQQVFNQNTGFVPQQQSSLIQTQQTESMATSVSSSPSLPTSPGDFADSNPFEERFPGGGTSPIISMIPRYPVTSRPQTSDINDKVNKYLSKLVDYFISNEMKSNKSLPQVLLHPPPHSAPYIDAPIDPELHTAFHWACSMGNLPIAEALYEAGTSIRSTNSQGQTPLMRSSLFHNSYTRRTFPRIFQLLHETVFDIDSQSQTVIHHIVKRKSTTPSAVYYLDVVLSKIKDFSPQYRIELLLNTQDKNGDTALHIASKNGDVVFFNTLVKMGALTTISNKEGLTANEIMNQQYEQMMIQNGTNQHVNSSNTDLNIHVNTNNIETKNDVNSMVIMSPVSPSDYITYPSQIATNISRNIPNVVNSMKQMASIYNDLHEQHDNEIKSLQKTLKSISKTKIQVSLKTLEVLKESSKDENGEAQTNDDFEILSRLQEQNTKKLRKRLIRYKRLIKQKLEYRQTVLLNKLIEDETQATTNNTVEKDNNTLERLELAQELTMLQLQRKNKLSSLVKKFEDNAKIHKYRRIIREGTEMNIEEVDSSLDVILQTLIANNNKNKGAEQIITISNANSHA BB7C35E29802BBD5 swi6_yeast Regulatory protein SWI6 SWI6 YLR182W L9470.8 Cell-cycle box factor subunit SWI6 Trans-acting activator of HO endonuclease gene MBF subunit P90 protein protein yeast Saccharomyces cerevisiae Baker's yeast MALEEVVRYLGPHNEIPLTLTRDSETGHFLLKHFLPILQQYHDTGNINETNPDSFPTDEERNKLLAHYGIAVNTDDRGELWIELEKCLQLLNMLNLFGLFQDAFEFEEPETDQDEEDPSHSKLPENKTKSENSKDNISSKRINNLQDMSLDSDAHRELGSPLKKLKIDTSVIDAESDSTPNTARGKPNDDINKGPSGDNENNGTDDNDRTAGPIITFTHDLTSDFLSSPLKIMKALPSPVVNDNEQKMKLEAFLQRLLFPEIQEMPTSLNNDSSNRNSEGGSSNQQQQHVSFDSLLQEVNDAFPNTQLNLNIPVDEHGNTPLHWLTSIANLELVKHLVKHGSNRLYGDNMGESCLVKAVKSVNNYDSGTFEALLDYLYPCLILEDSMNRTILHHIIITSGMTGCSAAAKYYLDILMGWIVKKQNRPIQSGTNEKESKPNDKNGERKDSILENLDLKWIIANMLNAQDSNGDTCLNIAARLGNISIVDALLDYGADPFIANKSGLRPVDFGAGTSKLQNTNGGDENSKMVSKGDYDGQKNGKAKKIRSQLLKNPPETTSLINDVQNLLNSISKDYENETVQYNEKLEKLHKELNEQREELANSREQLANVKQLKDEYSLMQEQLTNLKAGIEEEEESFREESKKLGIIADESSGIDWDSSEYDADEPFKVEFLSDFLEDKLQKNYEGDISKLLEAESKEQIMEQIRNQLPAEKIQSMLPPTVLLKARINAYKRNDKHLTNVLDTISTKQSELENKFRRVLSLCLKIDENKVDNMLDGLLQAISSEDPQDIDTDEMQDFLKKHAS 9B317FCACEEC493C mbp-probe MBP containing probe - TMP1 gene dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) MBP probe consisting of the TMPl promoter carrying two MCB elements swi6-mbp1-2 Components of the MBP transcription complex identified by EMSA 653049 N/A 653051 unspecified role unspecified role neutral component neutral component N/A 653052 unspecified role unspecified role neutral component neutral component N/A 653053 unspecified role unspecified role neutral component neutral component association association false false false Figure 4 mbp1-mbp-dna-1 Binding of Mbp1 to MCP elements in the TIMP1 gene transcription complex identified by EMSA 653049 N/A 653053 unspecified role unspecified role neutral component neutral component N/A 653051 unspecified role unspecified role neutral component neutral component n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 4B swi6-mbp1-1 Components of the MBP transcription complex identified by affinity chromatography 653050 N/A 653051 unspecified role unspecified role neutral component neutral component N/A 653052 unspecified role unspecified role neutral component neutral component physical association physical association false false false Figure 1