IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yeoh-2007-3 Subcellular discharge of a serine protease mediates release of invasive malaria parasites from host erythrocytes. in vitro In vitro protease assay protease assay fingerprinting peptide massfingerprinting fingerprinting Yeoh S., O'Donnell RA., Koussis K., Dluzewski AR., Ansell KH., Osborne SA., Hackett F., Withers-Martinez C., Mitchell GH., Bannister LH., Bryans JS., Kettleborough CA., Blackman MJ. Substrates tracked by fluorescently labelling. mblackm@nimr.mrc.ac.uk Cell (0092-8674) 2007 Only protein-protein interactions 8 imex curation yeoh-2007-1 Subcellular discharge of a serine protease mediates release of invasive malaria parasites from host erythrocytes. in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Yeoh S., O'Donnell RA., Koussis K., Dluzewski AR., Ansell KH., Osborne SA., Hackett F., Withers-Martinez C., Mitchell GH., Bannister LH., Bryans JS., Kettleborough CA., Blackman MJ. mblackm@nimr.mrc.ac.uk Cell (0092-8674) 2007 Only protein-protein interactions 8 imex curation yeoh-2007-2 Subcellular discharge of a serine protease mediates release of invasive malaria parasites from host erythrocytes. in vitro In vitro protease assay protease assay protein sequence protein sequence identification protein sequence Yeoh S., O'Donnell RA., Koussis K., Dluzewski AR., Ansell KH., Osborne SA., Hackett F., Withers-Martinez C., Mitchell GH., Bannister LH., Bryans JS., Kettleborough CA., Blackman MJ. mblackm@nimr.mrc.ac.uk Cell (0092-8674) 2007 Only protein-protein interactions 8 imex curation sera5_plaf7 Serine-repeat antigen protein 5 SERA5 111 kDa antigen Serine protease SERA5 p126 PFB0340c protein protein plaf7 Plasmodium falciparum (isolate 3D7) MKSYISLFFILCVIFNKNVIKCTGESQTGNTGGGQAGNTGGDQAGSTGGSPQGSTGASPQGSTGASPQGSTGASQPGSSEPSNPVSSGHSVSTVSVSQTSTSSEKQDTIQVKSALLKDYMGLKVTGPCNENFIMFLVPHIYIDVDTEDTNIELRTTLKKTNNAISFESNSGSLEKKKYVKLPSNGTTGEQGSSTGTVRGDTEPISDSSSSSSSSSSSSSSSSSSSSSSSSSSSESLPANGPDSPTVKPPRNLQNICETGKNFKLVVYIKENTLILKWKVYGETKDTTENNKVDVRKYLINEKETPFTNILIHAYKEHNGTNLIESKNYAIGSDIPEKCDTLASNCFLSGNFNIEKCFQCALLVEKENKNDVCYKYLSEDIVSKFKEIKAETEDDDEDDYTEYKLTESIDNILVKMFKTNENNDKSELIKLEEVDDSLKLELMNYCSLLKDVDTTGTLDNYGMGNEMDIFNNLKRLLIYHSEENINTLKNKFRNAAVCLKNVDDWIVNKRGLVLPELNYDLEYFNEHLYNDKNSPEDKDNKGKGVVHVDTTLEKEDTLSYDNSDNMFCNKEYCNRLKDENNCISNLQVEDQGNCDTSWIFASKYHLETIRCMKGYEPTKISALYVANCYKGEHKDRCDEGSSPMEFLQIIEDYGFLPAESNYPYNYVKVGEQCPKVEDHWMNLWDNGKILHNKNEPNSLDGKGYTAYESERFHDNMDAFVKIIKTEVMNKGSVIAYIKAENVMGYEFSGKKVQNLCGDDTADHAVNIVGYGNYVNSEGEKKSYWIVRNSWGPYWGDEGYFKVDMYGPTHCHFNFIHSVVIFNVDLPMNNKTTKKESKIYDYYLKASPEFYHNLYFKNFNVGKKNLFSEKEDNENNKKLGNNYIIFGQDTAGSGQSGKESNTALESAGTSNEVSERVHVYHILKHIKDGKIRMGMRKYIDTQDVNKKHSCTRSYAFNPENYEKCVNLCNVNWKTCEEKTSPGLCLSKLDTNNECYFCYV sub1_plaf7 Subtilisin-like protease 1 SUB1 PfSUB1 PF3D7_0507500 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MMLNKKVVALCTLTLHLFCIFLCLGKEVRSEENGKIQDDAKKIVSELRFLEKVEDVIEKSNIGGNEVDADENSFNPDTEVPIEEIEEIKMRELKDVKEEKNKNDNHNNNNNNISSSSSSSSNTFGEEKEEVSKKKKKLRLIVSENHATTPSFFQESLLEPDVLSFLESKGNLSNLKNINSMIIELKEDTTDDELISYIKILEEKGALIESDKLVSADNIDISGIKDAIRRGEENIDVNDYKSMLEVENDAEDYDKMFGMFNESHAATSKRKRHSTNERGYDTFSSPSYKTYSKSDYLYDDDNNNNNYYYSHSSNGHNSSSRNSSSSRSRPGKYHFNDEFRNLQWGLDLSRLDETQELINEHQVMSTRICVIDSGIDYNHPDLKDNIELNLKELHGRKGFDDDNNGIVDDIYGANFVNNSGNPMDDNYHGTHVSGIISAIGNNNIGVVGVDVNSKLIICKALDEHKLGRLGDMFKCLDYCISRNAHMINGSFSFDEYSGIFNSSVEYLQRKGILFFVSASNCSHPKSSTPDIRKCDLSINAKYPPILSTVYDNVISVANLKKNDNNNHYSLSINSFYSNKYCQLAAPGTNIYSTAPHNSYRKLNGTSMAAPHVAAIASLIFSINPDLSYKKVIQILKDSIVYLPSLKNMVAWAGYADINKAVNLAIKSKKTYINSNISNKWKKKSRYLH sera6_plaf7 Serine-repeat antigen protein 6 SERA6 Cysteine protease SERA6 PF3D7_0207500 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MICPIFFLYIINVLFTQYFIKCEGNKVTVISHNNGHNDNLDVNKNGVISQENVFDTSESLNLPSNKKVGSDDLNTTTISFTVPDNLENEVKVVSSSESGKGATVSHTKVTSEGLSDTQPNVTQSVSSSTHTPGSLDSTMSTEQHSSVSQSSLPTESSSETLNKATVPEIPIQINSGLLKNYNGVKVTGSCGSYFRVYLVPHILIYALTKYSVIQLESLFNDNARIDVEHKGELQNKCSEGYHFKLVVYITHNVLNLKWKTYKPNEESKSEDSDVRKYRIPKLERPFTSIQVYTANSKAGVIETKNYNIRTDIPDTCDAIATDCFLNGNVNIEKCFQCTLLVQKKDKSHECFKYVSSEMKKKMNEIKVKAQDDFNPNEYKLIESIDNILSKIYKKANKPFEISKDLINLEDLDYQFKNELLEYCKLLKKVDTSGTLEEYELGNAEDIYNNLTRLLKSHSDENIVTLQGKLRNTAICIKNVDEWILNKRGLTLPSESPSESSSKSDSYLNTFNDKDKNEDKDDMSKNSKEEFKNDDKENSDDQNNNDSNKKDDENNINNGDTNYVYDFDDDDYDNNSYEKDMYESPIKENKNGVIDLEKYGNQIKLKSPYFKNSKYCNYEYCNRWRDKTSCISQIEVEEQGNCGLCWIFASKLHFETIRCMRGYGHFRSSALYVANCSKRKPIDRCEEGSNPLEFLRILDEKKFLPLESNYPYSYTSAGNSCPKLPNSWTNLWGDTKLLFNKKVHRYIGNKGFISHETSYFKNNMDLFIDMVKREVQNKGSVIIYIKTQDVIGYDFNGKGVHSMCGDRTPDHAANIIGYGNYINKKGEKRSYWLIRNSWSYYWGDEGNFRVDMLGPKNCLYNFIHTVVFFKLDLGTIHVPKKKSWKKNVYFLRHNPDFMYSLYYNNYEPETSQDFESENDYDNAFVHGQSNESDETNKEGKNVHNSVEKKIQILHILKHIKDSQIKRGLVKYDNINETKDEHTCSRVNSQDAEKYEECKKFCLTKWNECKDHYSPGYCLTDLYKGEDCNFCYV o96164_plaf7 PF3D7_0207700 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MKIHIFLIATIYVLFSEKLIKWTTASTTQGGDTDTHPGTPPGEGSDVSQGAGQDASQGAGQDANPDPTLPKPPSPPADDTKDTGSQGDADSSSSKIEIPPLVKPENHKTIVSAMLKNYKGVKVTGTCGADFGLFLVPHIYVHVKSEDTEIELSSELAPPEMQTKFDKTQLKKFCVKDDTKKFDFIAYIYKDILVFKWKVYEEGLSKEQDVDEMKYLLPNLKQPITSIQVHSWTGTKESYILESKDYVLGEGMPEKCDAIATDCFLSGFTDIGKCFQCKLLMQEKNINDSCFKYVSSNQKELIKKQLKITAQDDEESSEYHLSESIKNLLKNIYKKNNDDNKKKELLHFENVNSALKSELLNYCNLLKEVNMNGVLKDHQLGNVQDVFNNLTKLLEEHKEENDNVLYHKMKNEALCLKNVNDWMKNKTGLLLPQLSYDLTYKNNNFTEFTQNKSYTSQNIVDKLYCNHEYCNRLKDHNNCISKINVEDQKNCALSWAFASKYHLETIKCMKGYEPLNASVLYVTNCLKNKNKDVCTEGSNPLVFLETIEEKGFLPTESNYPYDQSKVGDICPQLQNDWDNVFENTKVLDYNNGPFSVGTKGYIAYESEAFQKDMHSFVKLVKDEIMNKGSVIAYVKAENVLGYELNGKKVQNLCGDKTPDHVVNIVGYGNYINNKGEKKSYWIVRNSWGKYWGDDGYFKVDMYGPSTCEDNFIHTVVVFNVQVPINEKFDKKEHDIYNYYLKTSPEFYHNLYYKTFNSNKEEKSMNKNSYVYGQDTTPVENEAPRSGVQKPTELSSTESQTVSPPNESQTESLLSGGSQVTNPTLTQSTSSSSGQQETGPLSTQGLSPATGDPKGKEQEASPAEGLSGVLNPTKEVTSEEKIQIIHLLKHIKNSKIRRGLVKYNHEFEVGDNSCSRSTSKNAEMHDECVNICEKYWPECRGTAVPGYCLSTHDDKNECDFCYV sub1-sera-3 Cleavage of Sera by Sub1 684053 N/A 684056 enzyme target enzyme target substrate substrate neutral component neutral component region fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9TY95:p.Glu386Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false Q9TY95:p.Asp395Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9TY95:p.Ile882Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false Q9TY95:p.Ser891Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 684057 enzyme enzyme neutral component neutral component protein cleavage protein cleavage false false false Fig 5D and Supp. Fig. 5A sub1 Autocleavage of Sub1 684053 N/A 684057 enzyme enzyme neutral component neutral component N/A 684057 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8I0V0:p.Lys212Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain false region fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Fig 5D and Supp. Fig. 5A sub1-sera6-2 Cleavage of Sera6 by Sub1 684053 N/A 684058 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 684057 enzyme enzyme neutral component neutral component protein cleavage protein cleavage false false false Supp. Fig. 7 Cleavage of Q9TY96 sub1-sera4-2 Cleavage of Sera4 by Sub1 684053 N/A 684057 enzyme enzyme neutral component neutral component N/A 684059 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false plaf7 Plasmodium falciparum (isolate 3D7) protein cleavage protein cleavage false false false Supp. Fig. 7 Cleavage of O96164 sub1-sera-1 Cleavage of Sera by Sub1 684054 N/A 684057 enzyme enzyme neutral component neutral component N/A 684056 enzyme target enzyme target substrate substrate neutral component neutral component plaf7 Plasmodium falciparum (isolate 3D7) protein cleavage protein cleavage false false false Fig. 5A and 5B Cleavage of Sera to a P56 form via a P73 intermediate Inhibited by MRT12113, a compound originally purified natural products from temperate plants. sub1-sera4-1 Cleavage of Sera4 by Sub1 684054 N/A 684057 enzyme enzyme neutral component neutral component N/A 684059 enzyme target enzyme target substrate substrate neutral component neutral component plaf7 Plasmodium falciparum (isolate 3D7) protein cleavage protein cleavage false false false Fig. 6B Cleavage of O96164 sub1-sera6-1 Cleavage of Sera6 by Sub1 684054 N/A 684058 enzyme target enzyme target substrate substrate neutral component neutral component plaf7 Plasmodium falciparum (isolate 3D7) N/A 684057 enzyme enzyme neutral component neutral component protein cleavage protein cleavage false false false Fig. 6B Cleavage of Q9TY96 sub1-sera-2 Cleavage of Sera by Sub1 684055 N/A 684057 enzyme enzyme neutral component neutral component N/A 684056 enzyme target enzyme target substrate substrate neutral component neutral component plaf7 Plasmodium falciparum (isolate 3D7) protein cleavage protein cleavage false false false Fig. 5C Cleavage of Sera to a P56 form via a P73 intermediate