IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lermontova-2007-1 Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lermontova I., Schubert V., Bornke F., Macas J., Schubert I. lermonto@ipk-gatersleben.de. Arabidopsis inflorescence cDNA library Plant Mol. Biol. (0167-4412) 2007 Only protein-protein interactions imex curation lermontova-2007-2 Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1. tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Lermontova I., Schubert V., Bornke F., Macas J., Schubert I. BiFC assays were performed in Agrobacterium-infiltrated tobacco (Nicotiana benthamiana) leaves. lermonto@ipk-gatersleben.de. Arabidopsis inflorescence cDNA library Plant Mol. Biol. (0167-4412) 2007 Only protein-protein interactions imex curation q84tg5_arath H/ACA ribonucleoprotein complex non-core subunit NAF1 At1g03530 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVGFPANQAKTVEEELPLQVVADQDPEVKTFKDSFDFPSIDSFPDFDSITWSESNRNVEDFSIEDTDFDFFEYHMEIPQEDVVTVSDVMCPENQTTVNSESIEGKLEVFNDDMSGEVGVSSVRSESMVEVKPVLCDVMSEEVGVSSVEVEPNVCVEMSANGGDEPAVKDSEPVVSENSRSMEGETEPVVVSDASVACPTMDLDESGLKKTDEGLACSIEVGLEKVSLAVDDDEKSDEAKGEMDSAESESETSSSSASSSDSSSSEEEESDEDESDKEENKKEEKFEHMVVGKEDDLAGELEEGEIENLDEENGDDDIEDEDDDDDDDDDDDDDVNEMVAWSNDEDDDLGLQTKEPIRSKNELKELPPVPPVDVTLEPHHATLPVGVVLSVMSAQVIVEGMEKHSPLTEGSILWITEKRTPLGLVDEIFGPVKCPYYIVRFNSESEVPEGVCQGTPVSFVADFAQHILNIKELQKKGYDASGDNDEEIPDELEFSDDEKEAEYRRMQKLEKRGMMSDQKTGNTRNKKKKNRDPGRPTSSYSGEWTKNQGSSSLSSNRSDPQMGGPRPQMDGFPPNNAAWRPQSNQQNPYQLPPIPNQMGMQIPFMAMQNQNQMMFQPQFNGGQMPMPGGPGGLNFFPGQASAPWPAMVGQNCFNQQFGMGRGIQQQPLPNELSFNMFSQGLQMHPPQSQMHRPQSQMNPQFQMPPQFQPHQQSPMNPQYQMMHRPQSPANPQFQMQAQSDVRPLQSQIPQSPSDLQSPMEPQSQGFSSGQSSERGRGFHGRGRGRGRGRFGRGRGRGRQQSG cbf5_arath H/ACA ribonucleoprotein complex subunit 4 CBF5 CBF5 homolog Dyskerin NAP57 Nopp-140-associated protein of 57 kDa homolog Nucleolar protein NAP57 homolog F24I3.230 At3g57150 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAEVDISHSKKKKQDKTENDAADTGDYMIKPQSFTPAIDTSQWPILLKNYDRLNVRTGHYTPISAGHSPLKRPLQEYIRYGVINLDKPANPSSHEVVAWIKRILRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQGAGKEYVCVARLHSAVPDVAKVARALESLTGAVFQRPPLISAVKRQLRIRTIYESKLLEYDADRHLVVFWVSCEAGTYIRTMCVHLGLLLGVGGHMQELRRVRSGILGENNNMVTMHDVMDAQFVYDNSRDESYLRRVIMPLEMILTSYKRLVVKDSAVNAICYGAKLMIPGLLRFENDIDVGTEVVLMTTKGEAIAVGIAEMTTSVMATCDHGVVAKIKRVVMDRDTYPRKWGLGPRASMKKKLIADGKLDKHGKPNEKTPVEWSRNVVLPTGGDAIIAGAAAAPEEIKADAENGEAGEARKRKHDDSSDSPAPVTTKKSKTKEVEGEEAEEKVKSSKKKKKKDKEEEKEEEAGSEKKEKKKKKDKKEEVIEEVASPKSEKKKKKKSKDTEAAVDAEDESAAEKSEKKKKKKDKKKKNKDSEDDEE cbf5-at1g03530 Yeast two hybrid assay reveals interaction between CBF5 protein and At1g03530 protein 662849 N/A 662851 unspecified role unspecified role prey prey N/A 662852 unspecified role unspecified role bait bait physical association physical association false false false cbf5-at1g03530-1 BiFc assay reveals interaction between CBF5 protein and At1g03530 protein 662850 N/A 662852 unspecified role unspecified role neutral component neutral component region n-terminal region of the yfp protein yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 662851 unspecified role unspecified role neutral component neutral component region c-terminal region of the yfp protein yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 5 The authors report that colocalisation was observed in nucleoplasm, nuclear bodies and nucleolus.