MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint walfridsson-2007-1 A genome-wide role for CHD remodelling factors and Nap1 in nucleosome disassembly. schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Walfridsson J., Khorosjutina O., Matikainen P., Gustafsson CM., Ekwall K. julian.walfridsson@sh.se EMBO J. (0261-4189) 2007 2009-01-08 Only protein-protein interactions imex curation yco6_schpo Putative nucleosome assembly protein C364.06 SPCC364.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVENVNINNKRAGKMSAAPTPHNTPSGTSAPNFGAKAPQVNTIDEGDENLEAIDGKLGSLLHLTSEGVSELPEAVQRRISGLRGLQKRYSDLESQFQKELFELEKAYAKKYAPIFKRRSEVVRGADEPTEEEIKKGEAADENEKKEPTSSESKKQEGGDDTKGIPEFWLTAMKNVLSLSEMITPEDEGALSHLVDIRISYMEKPGFKLEFEFAENPFFTNKILTKTYYYMEESGPSNVFLYDHAEGDKVDWKENADLTVRTVTKKQRNKNTKQTRVVKVSVPRDSFFNFFNPPTPPSEEDEESESPELDELLELDYQIGEDFKEKLIPRAVEWFTGEALALENYDGFSDLDVEEDEDDVESSSNEEVSDSDEEDSDSKHTAHGQQNAAECRQQ mint hrp3_schpo Chromodomain helicase hrp3 hrp3 SPAC3G6.01 ATP-dependent helicase hrp3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSTSAIALALSSSKAIEQLDHVQTETPNLKQEMSESPSNSGVASKRKLQSTEWLDPELYGLRRSGRTRSNPGRYVDTDDQEDVFPSKHRKGTRNGSSFSRHRTIRDLDDEAESVTSEESESDDSSYGGTPKKRSRQKKSNTYVQDEIRFSSRNSKGVNYNEDAYFESFEEEEEEEMYEYATEVSEEPEDTRAIDVVLDHRLIEGHDGSTPSEDYEFLIKWVNFSHLHCTWEPYNNISMIRGSKKVDNHIKQVILLDREIREDPTTTREDIEAMDIEKERKRENYEEYKQVDRIVAKHLNSDGSVEYLVKWKQLLYDFCTWEASSIIEPIAATEIQAFQEREESALSPSRGTNYGNSRPKYRKLEQQPSYITGGELRDFQLTGVNWMAYLWHKNENGILADEMGLGKTVQTVAFLSYLAHSLRQHGPFLVVVPLSTVPAWQETLALWASDMNCISYLGNTTSRQVIRDYEFYVDGTQKIKFNLLLTTYEYVLKDRSVLSNIKWQYMAIDEAHRLKNSESSLYEALSQFKNSNRLLITGTPLQNNIRELAALVDFLMPGKFEIREEINLEAPDEEQEAYIRSLQEHLQPYILRRLKKDVEKSLPSKSERILRVELSDLQMYWYKNILTRNYRVLTQSISSGSQISLLNIVVELKKASNHPYLFDGVEESWMQKINSQGRRDEVLKGLIMNSGKMVLLDKLLSRLRRDGHRVLIFSQMVRMLDILGDYLSLRGYPHQRLDGTVPAAVRRTSIDHFNAPNSPDFVFLLSTRAGGLGINLMTADTVIIFDSDWNPQADLQAMARAHRIGQKNHVMVYRLLSKDTIEEDVLERARRKMILEYAIISLGVTDKQKNSKNDKFSAEELSAILKFGASNMFKAENNQKKLEDMNLDEILEHAEDHDTSNDVGGASMGGEEFLKQFEVTDYKADVSWDDIIPLTEREKFEEEDRLREEEEALKQEIELSSRRGNRPYPSSAVESPSYSGTSERKSKKQMLKDEVLLEKEIRLLYRAMIRYGSLEHRYNDIVKYADLTTQDAHVIKKIAADLVTASRKAVSAAEKDLSNDQSNNKSSRKALLITFKGVKNINAETLVQRLNDLDILYDAMPTSGYSNFQIPMHVRSVHGWSCQWGPREDSMLLSGICKHGFGAWLEIRDDPELKMKDKIFLEDTKQTDNSVPKDKENKEKKVPSAVHLVRRGEYLLSALREHHQNFGIKSSPAISTNGKTQPKKQTANRRQSGKPNVKSAQKIESATRTPSPAISESRKKPSSKDTKIETPSREQSRSQTASPVKSEKDDGNVSLNAEQKARCKELMYPVRKHMKRLRKDSSGLGRAELVKLLTECLTTIGKHIEKTVNDTPSEEKATVRKNLWMFACYFWPKEEVKYTSLISMYEKMK mint Involved in heterochromatin silencing. Required for transcriptional repression at the silence loci of mat3, where it has a direct role as a chromatin remodeling factor eif3d_schpo Eukaryotic translation initiation factor 3 subunit D moe1 SPAC637.07 eif3d Microtubule-destabilizing protein moe1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MATGFKLPELAPVKSAWGPPETEQIGGDIPYAPFSKGDRLGKIADWSVDQPKDGREQRGRQGAFAGRFRDQYQTYGYGASSIFGYQHSEDESSFSVIDRGSVNRTRTSARNGGTLLKVRGRGQNVQRGGRGGRYGSSGGRGAGDTVVSRSSGAGGARGRRFGWKDYDKHQRLRNASVTVGDDWQLLDEVEFSHLSKLNLAAAAPVTVDSYGYIYPYDKSFDKIHVKSEKPLQALDRVHYNPTTTEDPVIQKLALNSDANIFITDSILSLLMCSTRSVYPWDIVITHQSGKLFFDKREGGPFDYLTVNENAYDSPMDADNREGVNSPGALSVEATYINQNFCVQALRETEEEKYKLPHPNPFYNSKEQSEPLAAHGYIYRDVDLSLETDEKPVKLMVRTEVDGYVKNPANDVQYISIKALNEYDPKFTNVTGSVDWRSKLESQRGAVFATEMKNNSCKLARWTVEALLAGVDSMKVGFVSRSNARDAQHHGILGVVAYKPADLASQMNLSLSNGWGIVRTIADVCLKMPDGKYVLVKDPNRPILRLYSVPPNTFEEAAGPSLEASSTA mint Binds to the 40S ribosome. Induces instabilty in microtubules. Affect spingle formation fes1_schpo Hsp70 nucleotide exchange factor fes1 fes1 SPBC3B9.01 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEKLLAFSTQLQAQGNSSNSPPDPKDLDPSVLDHIFGANPADEMRKAMDAIEDPSVPLDQKEIAFDNLEMLVEHIDNANNLVPLQLWPRLLKQLESPESTLRRLAAWTIATAVQNNPKSQQALIENDGLKILFGALKKEDSDETKNKVLYAITSELKLNEAGIALLDKIPNSWEMLIEILELKHSVMTKRVIFFFYALLIQEDKSKQIILQKAHEFQIPEKVYQFSLEHSVDEDCVTKSLHTLYLFQKNKVSVANTNELLKSLVQFKSEFPEIFTVDEWKAFHEALE mint Functions as a nucleotide exchange factor (NEF) for Hsp70 chaperones which accelerates the release of ADP. Required for fully efficient Hsp70-mediated folding of proteins eif3g_schpo Eukaryotic translation initiation factor 3 subunit G tif35 SPBC18H10.03 eif3g Translation initiation factor eIF3 p33 subunit homolog Eukaryotic translation initiation factor 3 RNA-binding subunit protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSSKSLDWADDEDYGTGLPSIQTFDNPDGTKTMIEFRIDDNGKKVKVTRVIRKTVITERVQHAVAERKKWKKFGKEAGKNSGVDARTTSVGENVQLRLQLGWTTTKEEEQDEAALAAAKVKAKGSSVVRCRACKGNHFTAQCPYKSIIGPVDEPPLDASPVSSRASGALGEKGRYIAPHLRAGSGRESGDSMFKRERDDSATLRVTNLSDDTREEELRDLFRRFGGIQRVYLAKDKETGRAKGFAFVSYYDRDCAIKARDRLDGYGWNNLILRCEFSKPRD Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. This subunit binds to the 18S rRNA mint spt16_schpo FACT complex subunit spt16 spt16 SPBP8B7.19 Facilitates chromatin transcription complex subunit spt16 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAEYEIDEITFHKRLGILLTSWKNEEDGKTLFQDCDSILVTVGAHDDTNPYQKSTALHTWLLGYEFPSTLILLEKHRITILTSVNKANMLTKLAETKGAAADVNILKRTKDAEENKKLFEKIIEYIRATNKKVGVFPKDKTQGKFINEWDSIFEPVKSEFNLVDASLGLAKCLAIKDEQELANIKGASRVSVAVMSKYFVDELSTYIDQGKKITHSKFSDQMESLIDNEAFFQTKSLKLGDIDLDQLEWCYTPIIQSGGSYDLKPSAITDDRNLHGDVVLCSLGFRYKSYCSNVGRTYLFDPDSEQQKNYSFLVALQKKLFEYCRDGAVIGDIYTKILGLIRAKRPDLEPNFVRNLGAGIGIEFRESSLLVNAKNPRVLQAGMTLNLSIGFGNLINPHPKNSQSKEYALLLIDTIQITRSDPIVFTDSPKAQGDISYFFGEDDSSLEDGVKPRKPPTRGTATISSHKGKTRSETRDLDDSAEKRRVEHQKQLASRKQAEGLQRFAQGSVPSSGIEKPTVKRFESYKRDSQLPQAIGELRILVDYRAQSIILPIFGRPVPFHISTLKNASKNDEGNFVYLRLNFVSPGQIGGKKDELPFEDPNAQFIRSFTFRSSNNSRMSQVFKDIQDMKKAATKRETERKEFADVIEQDKLIEIKNKRPAHINDVYVRPAIDGKRLPGFIEIHQNGIRYQSPLRSDSHIDLLFSNMKHLFFQPCEGELIVLIHVHLKAPIMVGKRKTQDVQFYREVSDIQFDETGNKKRKYMYGDEDELEQEQEERRRRAQLDREFKSFAEKIAEASEGRIELDIPFRELAFNGVPFRSNVLLQPTTDCLVQLTDTPFTVITLNEIEIAHLERVQFGLKNFDLVFIFQDFRRPPIHINTIPMEQLDNVKEWLDSCDICFYEGPLNLNWTTIMKTVNEDPIAFFEEGGWGFLGAPSDDEGDDSVEEVSEYEASDADPSDEEEEESEEYSEDASEEDGYSESEVEDEESGEDWDELERKARQEDAKHDAFEERPSKKRHR 50AD706DD6C03313 mint Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishement of the nucleosome following the passage of RNA polymerase II nol10_schpo Ribosome biogenesis protein enp2 homolog SPCC330.09 enp2 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLKVQNPNNVRVYTVSGEGVTQRLPNWISKRKLKKDYALSHRIELLQDFEYPEASNRIKCTRDGKYAMATGVYKPHIKVFDFAEMSLKFERHTDAENVQFEILSDDWTKSVHLQTDRTVDFHSQGGIHYSTRIPKYGRDLKYHYPNCDLYLAAAGDEVYRLNLEQGRFLNPLKIESAQIDSPTGGVNVIDINPMHQLLAFGTDAGSVEFWDPRDRSRVGILEIPSTVPSTPYSDNSRSVTALKYRNDGLNVAIGLSDGATLLYDLRSSSPYMSKDQGYSMPIKSLHWMDSALDGTARVLSADSKIIKIWEKDTGKPFSSIEPTVELNDVCPIEGTGLILTANEGSPMHAFYIPSLNPAPRWCSFLDNITEEMEENPAPTIYDDYKFVTKKELLNLGLDHLVGTGVIRAYMHGFFIDNNLYEKARLIANPFSYEEHRQKIVKERLEKQRASKIRSQNRPKVNAGLASRLSYQENKLRKKTGVTDGPSILEDERFKNVFTDKEFEVDEDTLEYKQLHPSRSEARGLTAAEESEEEKEHTKGIKFSSDEESLSDMEEENETFDALVDRRKTEQQVSNEKTPQETIRSTPSGMEMTFKVEKKKKSKPVNRDEDSTSGKKKQVTQGRRSASKNVFRNM May be involved in rRNA-processing and ribosome biosynthesis mint hrp1_schpo Chromodomain helicase hrp1 hrp1 SPAC1783.05 ATP-dependent helicase hrp1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEPEHSNYDLKNHPTSVQESTLNGSAVTDPQFGNTTNSLPSMNGLLNHENSFASQQSLSSSAFDDSEIVTSTANSTVVSSALSTPKIDDAQNSDDVRVDGTRRSSRAKRPVYRDFSYTEIDEHEIPIPKKRKSKPAPKQKKSVASDDEDAYDKRHRFSINSASGTEIRTSLRSSKGKSVNYNEQEFYDDFEDEEEEVEEQVEEEYEPIIDFVLNHRKRADAQDDDPKSSYQYLIKWQEVSHLHNTWEDYSTLSSVRGYKKVDNYIKQNIIYDREIREDPTTTFEDIEALDIERERKNMLFEEYKIVERIVASETNEEGKTEYFVKWRQLPYDNCTWEDADVIYSMAPNEVYQFLQRENSPYLPYKGVFYNTRPPYRKLEKQPSYIKGGEIRDFQLTGINWMAYLWHRNENGILADEMGLGKTVQTVCFLSYLVHSLKQHGPFLIVVPLSTVPAWQETLANWTPDLNSICYTGNTESRANIREYEFYLSTNSRKLKFNILLTTYEYILKDKQELNNIRWQYLAIDEAHRLKNSESSLYETLSQFRTANRLLITGTPLQNNLKELASLVNFLMPGKFYIRDELNFDQPNAEQERDIRDLQERLQPFILRRLKKDVEKSLPSKSERILRVELSDMQTEWYKNILTKNYRALTGHTDGRGQLSLLNIVVELKKVSNHPYLFPGAAEKWMMGRKMTREDTLRGIIMNSGKMVLLDKLLQRLKHDGHRVLIFSQMVRMLNILGEYMSLRGYNYQRLDGTIPASVRRVSIDHFNAPDSPDFVFLLSTRAGGLGINLNTADTVIIFDSDWNPQADLQAMARAHRIGQKNHVNVYRFLSKDTVEEDILERARRKMILEYAIISLGVTEKSKNSKNDKYDAQELSAILKFGASNMFKATENQKKLENMNLDDILSHAEDRDSSNDVGGASMGGEEFLKQFEVTDYKAEDLNWDDIIPEEEMERIEEEERMLAAQRAKEEERERREEEERENDEDHPSRTYKRTTKSITKRQQRREEMVREKEIRLLYRAMIKFGLVDERFDTIVKEAELQATDPKRIYSLSADMVKACDEAVERLGADDTKNKQPRKAILIEFKGVKNINAETVTLRVKDLTHLHRAYKGLDPLKQIIGYPIRSVHSWNCSWGIKEDSMLLAGINKHGFGCWQAIKNDPDLGLHDKIFLDEAKNDKESRYVPSAVHLVRRGEYLLSVVREHPDLFVVKTDQPTKRKYNRKAPTKSSTRQTTLDGSISNTKKSSRTKKKKEEETNRGDETSPEGTVGEDEVEEEPRQAEPPKRALRSNSGKAASNKRTTRNSMKTHSAMDTLTAVAALDAELDNMSNEKAKEEVDHVKSENGESVNEPNTEDLSLETEENTTVSDISPLVKNEA mint Seems to play a role in mitotic chromosome segregation and maintenance of chromatin structure. Has ATPase activity hrp1-enp2 664826 yco6_schpo Nap1 664827 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level hrp3_schpo hrp3 664828 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level eif3d_schpo moe1 664829 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level fes1_schpo Hsp70 664830 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level eif3g_schpo eIF3 664831 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level spt16_schpo cdc68 664832 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level nol10_schpo SPCC330.09 664833 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level hrp1_schpo hrp1 664834 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false F1A mint yco6-hrp1 664826 hrp1_schpo hrp1 664834 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tagged myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yco6_schpo Nap1 664827 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tap tagged tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false F1C mint yco6-hrp3 664826 hrp3_schpo hrp3 664828 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tagged myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yco6_schpo Nap1 664827 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tap tagged tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false F1C mint