IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ allingham-2007-1 Vik1 modulates microtubule-Kar3 interactions through a motor domain that lacks an active site. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Allingham JS., Sproul LR., Rayment I., Gilbert SP. A unique UniProtKB entry could not be associated with conventional kinesin K401 since organism andexact gene product of interactor cannot be ascertained. ivan_rayment@biochem.wisc.edu,spg1@pitt.edu Cell (0092-8674) 2007 Only protein-protein interactions 3 imex curation allingham-2007-2 Vik1 modulates microtubule-Kar3 interactions through a motor domain that lacks an active site. in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation predetermined predetermined participant predetermined Allingham JS., Sproul LR., Rayment I., Gilbert SP. A unique UniProtKB entry could not be associated with conventional kinesin K401 since organism andexact gene product of interactor cannot be ascertained. ivan_rayment@biochem.wisc.edu,spg1@pitt.edu Cell (0092-8674) 2007 Only protein-protein interactions 3 imex curation vik1_yeast Spindle pole body-associated protein VIK1 YPL253C VIK1 Vegetative interaction with KAR3 protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MASQQNKHAFLSKNRIFHNPDNVSSSKSRNLMDITNTTNTMNGSRPSSMKSSLALPPVKDSFPSVSRSASLNINMSKIKDLKDRQDKIRFQRHTLRTQLIECEREIKTIKFRDLNKSRFELYKKKSKQAKYLKQVRDLTQNLNSKDGERADLIKKNKSALATLQAELDQNLILKRQESQELYNNKLIFWENELQIMENVEPDHEITEEISQLKKTLQELNINWANLQKQNLERQVNHESQLRKDFIAFKEAKLKSMENLTNKHRELLDQIATLQSESEKLHKEIMDIDRQAEYSEQNISEINENIKQLELANNPLISKSLQNSQDLEHLQNQMENLKEMASKQEKFYNDTYNTVEKELLRSRRLENSIIEQKGTMRCYAYVMEQNLPENLLFDYENGVITQGLSEHVYKFNRVIPHLKVSEDKFFTQEYSVYHDMCLNQKKNFNLISLSTTPHGSLRESLIKFLAEKDTIYQKQYVITLQFVFLSDDEFSQDMLLDYSHNDKDSIKLKFEKHSISLDSKLVIIENGLEDLPLNFSCDEHPNLPHSGMGIIKVQFFPRDSKSDGNNDPVPVDFYFIELNNLKSIEQFDKSIFKKESCETPIALVLKKLISDTKSFFLLNLNDSKNVNKLLTISEEVQTQLCKRKKKLT kar3_yeast Kinesin-like protein KAR3 KAR3 YPR141C P9659.16 Nuclear fusion protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MESLPRTPTKGRSTQHLSTPSPKNDILAMNGHKRRNTTTPPPKHTLLKPQRTDIHRHSLASQSRISMSPNRELLKNYKGTANLIYGNQKSNSGVTSFYKENVNELNRTQAILFEKKATLDLLKDELTETKEKINAVNLKFETLREEKIKIEQQLNLKNNELISIKEEFLSKKQFMNEGHEIHLKQLAASNKKELKQMENEYKTKIEKLKFMKIKQFENERASLLDKIEEVRNKITMNPSTLQEMLNDVEQKHMLEKEEWLTEYQSQWKKDIELNNKHMQEIESIKKEIENTLKPELAEKKKLLTEKRNAYEAIKVKVKEKEEETTRLRDEVALKQKTNLETLEKIKELEEYIKDTELGMKELNEILIKEETVRRTLHNELQELRGNIRVYCRIRPALKNLENSDTSLINVNEFDDNSGVQSMEVTKIQNTAQVHEFKFDKIFDQQDTNVDVFKEVGQLVQSSLDGYNVCIFAYGQTGSGKTFTMLNPGDGIIPSTISHIFNWINKLKTKGWDYKVNCEFIEIYNENIVDLLRSDNNNKEDTSIGLKHEIRHDQETKTTTITNVTSCKLESEEMVEIILKKANKLRSTASTASNEHSSRSHSIFIIHLSGSNAKTGAHSYGTLNLVDLAGSERINVSQVVGDRLRETQNINKSLSCLGDVIHALGQPDSTKRHIPFRNSKLTYLLQYSLTGDSKTLMFVNISPSSSHINETLNSLRFASKVNSTRLVSRK cik1_yeast Spindle pole body-associated protein CIK1 CIK1 YMR198W YM9646.11 Chromosome instability and karyogamy protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNNSKIPKLSFHSDPNNVTRDFPKTKRQKVQKREMDMILTPNNNKLNILHSSGSGIRRCYTDDTSATYTKKLTFGGDPKIIERVKNNERKVRKDIDSLLNAISEIEKESVRIHARELPAITLELDAKVKACRELQNEIDGLSTEMDLKDNQCDLQRKNVELSSKNIVSMHAVKVQEFENDLEEELSNAKREWTYKLMEVENLKPDERLTDEMRQLKTEFEEVNRKLFILQNENENECKNYKKELDKKFEIFKKVKNDARIELDGEQERLSKVLKDLQDTHGELKENIKTCRDEFNDFEKRIGEAEVNFHSMELAVVPLKKKLASTSQALTQVQEEKKQVEGEANNWKKKYVNELEKVQQELYTRQNLATSIEEIKGYTRCFAYANERQMPDEFHINYVDRCICENSGEKRVQVFDRVVLEEIHKDHKRLYNECIPFLEKYISKLINCSIIVVSQQPTAPMKKTLLKQLIEQYGENYKMTLNILHLDGSIKHSDVGLDNPTEIRDLSQDEECMNILTLDTKLGKDEESHSMNIYIGSMSTVQLNRELDDAPSVLSHILTKTKQCFVFKINAGENIEKALALAGKLKRTITLPQLD A2A09DEB8C2EC838 kar3-vik1 Kar3 and Vik1 form a heterodimer as confirmed by analytic gel filtration and Coomassie gel 599467 N/A 599469 unspecified role unspecified role neutral component neutral component c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 N/A 599470 unspecified role unspecified role neutral component neutral component c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 physical association physical association false false false S1 cik1-kar3 Kar3 and Cik1 form a heterodimer as confirmed by analytic gel filtration and Coomassie gel 599467 N/A 599471 unspecified role unspecified role neutral component neutral component c-terminal sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) N/A 599470 unspecified role unspecified role neutral component neutral component c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 physical association physical association false false false S1 kar3-vik1-1 Kar3 and Vik1 form a heterodimer as confirmed by sedimentation equilibrium 599468 N/A 599469 unspecified role unspecified role neutral component neutral component c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 N/A 599470 unspecified role unspecified role neutral component neutral component c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 physical association physical association false false false S5