IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kehn-2007-1 Functional consequences of cyclin D1/BRCA1 interaction in breast cancer cells. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells confocal microscopy confocal microscopy predetermined predetermined participant predetermined Kehn K., Berro R., Alhaj A., Bottazzi ME., Yeh WI., Klase Z., Van Duyne R., Fu S., Kashanchi F. Cancer - Interactions investigated in the context of cancer A unique UniProtKB entry could not be associated with the recombinant cyclin D1 and recombinant BRCA1 since the organism of origin of these interactors could not be ascertained. Thus the pull down experiments and in vitro kinase assays in Fig. 4 could not be curated. The in vitro kinase assays in Fig. 5 could not be curated as it is not clear which protein(s) is carrying out the phosphorylations. The ChIP assays were carried out on cell extracts and thus could not be curated, as it is not clear which proteins were involved in nucleic acid binding. bcmfxk@gwumc.edu Oncogene (0950-9232) 2007 Only protein-protein interactions imex curation kehn-2007-2 Functional consequences of cyclin D1/BRCA1 interaction in breast cancer cells. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kehn K., Berro R., Alhaj A., Bottazzi ME., Yeh WI., Klase Z., Van Duyne R., Fu S., Kashanchi F. Cancer - Interactions investigated in the context of cancer bcmfxk@gwumc.edu Oncogene (0950-9232) 2007 Only protein-protein interactions imex curation A unique UniProtKB entry could not be associated with the recombinant cyclin D1 and recombinant BRCA1 since the organism of origin of these interactors could not be ascertained. Thus the pull down experiments and in vitro kinase assays in Fig. 4 could not be curated. The in vitro kinase assays in Fig. 5 could not be curated as it is not clear which protein(s) is carrying out the phosphorylations. The ChIP assays were carried out on cell extracts and thus could not be curated, as it is not clear which proteins were involved in nucleic acid binding. kehn-2007-3 Functional consequences of cyclin D1/BRCA1 interaction in breast cancer cells. human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kehn K., Berro R., Alhaj A., Bottazzi ME., Yeh WI., Klase Z., Van Duyne R., Fu S., Kashanchi F. Cancer - Interactions investigated in the context of cancer bcmfxk@gwumc.edu Oncogene (0950-9232) 2007 Only protein-protein interactions imex curation A unique UniProtKB entry could not be associated with the recombinant cyclin D1 and recombinant BRCA1 since the organism of origin of these interactors could not be ascertained. Thus the pull down experiments and in vitro kinase assays in Fig. 4 could not be curated. The in vitro kinase assays in Fig. 5 could not be curated as it is not clear which protein(s) is carrying out the phosphorylations. The ChIP assays were carried out on cell extracts and thus could not be curated, as it is not clear which proteins were involved in nucleic acid binding. brca1_human Breast cancer type 1 susceptibility protein BRCA1 RNF53 RING finger protein 53 RING-type E3 ubiquitin transferase BRCA1 protein protein human Homo sapiens Human MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY ccnd1_human G1/S-specific cyclin-D1 CCND1 BCL1 PRAD1 PRAD1 oncogene BCL-1 oncogene B-cell lymphoma 1 protein protein protein human Homo sapiens Human MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI brca1-ccnd1 BRCA1 and cyclin D1 colocalize in MCF-7 cells 661538 N/A 661541 unspecified role unspecified role neutral component neutral component N/A 661542 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2 These proteins colocalized in both cytoplasm and nucleus in asynchronous cells and mostly in the nucleus after hydroxyurea treatment (when blocked in early S phase). brca1-ccnd1-1 BRCA1 and cyclin D1 coimmunoprecipitate in MCF-7 cell lysates 661539 N/A 661541 unspecified role unspecified role bait bait N/A 661542 unspecified role unspecified role prey prey physical association physical association false false false 1 B, 3 D The association between BRCA1 and cyclin D1 is prominent in early G1 and less so at early S phase. This interaction was specific since both BRCA1 and cyclin D1 peptides competed out the antibodies in the immunoprecipitation reaction. brca1-ccnd1-2 BRCA1 and cyclin D1 coimmunoprecipitate in T47-D lysates 661540 N/A 661541 unspecified role unspecified role bait bait N/A 661542 unspecified role unspecified role prey prey physical association physical association false false false 1 B This interaction was specific since competing peptides competed out the antibodies in the immunoprecipitation reaction.