IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ shen-2007-1 Essential role for nuclear PTEN in maintaining chromosomal integrity. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis yy151@columbia.edu Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation Cancer - Interactions investigated in the context of cancer shen-2007-5 Essential role for nuclear PTEN in maintaining chromosomal integrity. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis yy151@columbia.edu Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation Cancer - Interactions investigated in the context of cancer shen-2007-2 Essential role for nuclear PTEN in maintaining chromosomal integrity. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis yy151@columbia.edu Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation Cancer - Interactions investigated in the context of cancer shen-2007-3 Essential role for nuclear PTEN in maintaining chromosomal integrity. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis yy151@columbia.edu Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation Cancer - Interactions investigated in the context of cancer shen-2007-4 Essential role for nuclear PTEN in maintaining chromosomal integrity. human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis The two primers were used in BLAST searches to find the DNA sequence. yy151@columbia.edu Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation Cancer - Interactions investigated in the context of cancer pten_mouse Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Mutated in multiple advanced cancers 1 Pten Mmac1 Phosphatase and tensin homolog protein protein mouse Mus musculus Mouse MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHSQITKV cenpc_mouse Centromere protein C Cenpc Cenpc1 Centromere autoantigen C Centromere protein C 1 protein protein mouse Mus musculus Mouse MASFHLDHLKNYHRRYCRSSRAPNIHTKKGQNMLEILQDCFEDQSKASFLDDFTESLTSSTQKKKANYSQSSSKKCPESHSKPVPVSSRTGEASLQASAEPSEAAGGSVQANEVHHGASDELDLCVGSPVVLLDANVNTLQKAASPAGQKRVASVSRSPVDRQASNKNISFKTRKRLNFEDKVTLSTAETENSVLQVEDNLSKGQEGTSSEITQKRDDLSSDVQSRSKKNFSELFLETVKRKSKSSSVVRHTAAVPFSPPPPSDMKLLEDEFIIDRSDRSFSSRLWVMIPSKDRHLSAHKPSPENTALLQGKKSREKSHSLSAMTFARNTQSDKAHPIEEAQLSVEENPATTCTDELENDCRSPENKMQSETAKTPPAWERTTKQSQRRVSKPKAAEELRKGQSSWENSNVSNTGQDKLQINSKRNMKDCEEVRNEPNPKKQKPALENKKKTNSTQTNKEKSGKKFFSGGSKNKFVPKKVTLTSRRSCRISQRPSEWWRVKSDESSVDRNPSKENNSPVVYPNKKKQTKRNHVSKRAGKKPGSSKRQKTEMSPRVQKSLNVKDSGGTVSGHDDTSRSQRKPLKIIEADPTQKSLAISRPKRGCKYRNNVMTSPNVHLKSHTEEYTSKTQMESASNSEMSKRSVWEESGPSRFKNYEMPGSSNSEMGDEQDQKSLHFTTRSFNMVPDKKLHHKLVLPSNSPNVRRSNRIRLKPLEYWRGERVDYQESSSGQLVLEIISPSSVPTKIKAQRNLGKVNKKVTKKPTHLNSHEKAKMELPLDMRLGDPFQATLAKDPETAELVPMDLIRPRDTYRFFVEQHGLKVFKTLDTIYFSTGKLVLGPYEEKGKQHVGQDILVFYVNFGDLLCTLHETPYKLTTGDSFYVPSGNHYNIKNLLNVESSLLFTQIKR rad51_mouse_gene DNA repair protein RAD51 homolog 1 Rad51 Rad51a Reca RAD51 homolog A gene gene mouse Mus musculus Mouse e2f1_mouse Transcription factor E2F1 E2f1 protein protein mouse Mus musculus Mouse MAVAPAGGQHAPALEALLGAGALRLLDSSQIVIISTAPDVGAPQLPAAPPTGPRDSDVLLFATPQAPRPAPSAPRPALGRPPVKRRLDLETDHQYLAGSSGPFRGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSRSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTMVGIGKRLEGLTQDLQQLQESEQQLDHLMHICTTQLQLLSEDSDTQRLAYVTCQDLRSIADPAEQMVIVIKAPPETQLQAVDSSETFQISLKSKQGPIDVFLCPEESADGISPGKTSCQETSSGEDRTADSGPAGPPPSPPSTSPALDPSQSLLGLEQEAVLPRMGHLRVPMEEDQLSPLVAADSLLEHVKEDFSGLLPGEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF pten_human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog Mutated in multiple advanced cancers 1 PTEN MMAC1 TEP1 protein protein human Homo sapiens Human MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV rad51_human_gene RAD51_HUMAN_GENE gene gene human Homo sapiens Human pten-cenpc1 CENP-C coimmunoprecipiates with PTEN in MEF lysates 577024 N/A 577029 unspecified role unspecified role bait bait N/A 577030 unspecified role unspecified role prey prey physical association physical association false false false 2 C, 4 G The PTEN-CENP-C interaction is markedly disrupted in MEFs also expressing human PTEN (aa 1-188). cenpc1-pten PTEN coimmunoprecipiates with CENP-C in MEF lysates 577024 N/A 577030 unspecified role unspecified role bait bait N/A 577029 unspecified role unspecified role prey prey physical association physical association false false false 2 D This interaction occurs in p53+/+ and p53-/- MEFs. pten-rad51-1 PTEN binds to the RAD51 promoter in MEFs 577025 n/a 577031 unspecified role unspecified role prey prey N/A 577029 unspecified role unspecified role bait bait association association false false false 5 G The primers given did not match the mouse RAD51 gene in a BLAST search, although they did match the human RAD51 gene. e2f1-rad51-1 E2F1 binds to the RAD51 promoter in MEFs 577025 n/a 577031 unspecified role unspecified role prey prey N/A 577032 unspecified role unspecified role bait bait association association false false false 7 C The primers given did not match the mouse RAD51 gene in a BLAST search, although they did match the human RAD51 gene. cenpc1-pten-1 PTEN coimmunoprecipiates with CENP-C in MEF lysates 577026 N/A 577033 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 577030 unspecified role unspecified role bait bait physical association physical association false false false 3 B, C P60484 tranfection pten-pten Endogenous PTEN coimmunoprecipiates with human HisPTEN (aa 1-188) in MEF lysates 577027 N/A 577029 unspecified role unspecified role prey prey N/A 577033 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4 H P60484 transfection pten-rad51_human_dna PTEN binds to the RAD51 promoter in PC-3 cells 577028 n/a 577034 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 577033 unspecified role unspecified role bait bait association association false false false 6 F