IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yun-2007-6 The novel cellular mechanism of human 5-HT6 receptor through an interaction with Fyn. crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yun HM., Kim S., Kim HJ., Kostenis E., Kim JI., Seong JY., Baik JH., Rhim H. hrhim@kist.re.kr Human brain cDNA J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation yun-2007-1 The novel cellular mechanism of human 5-HT6 receptor through an interaction with Fyn. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yun HM., Kim S., Kim HJ., Kostenis E., Kim JI., Seong JY., Baik JH., Rhim H. hrhim@kist.re.kr Human brain cDNA J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation yun-2007-2 The novel cellular mechanism of human 5-HT6 receptor through an interaction with Fyn. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yun HM., Kim S., Kim HJ., Kostenis E., Kim JI., Seong JY., Baik JH., Rhim H. hrhim@kist.re.kr Human brain cDNA J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation yun-2007-3 The novel cellular mechanism of human 5-HT6 receptor through an interaction with Fyn. in vitro In vitro pull down pull down nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yun HM., Kim S., Kim HJ., Kostenis E., Kim JI., Seong JY., Baik JH., Rhim H. hrhim@kist.re.kr Human brain cDNA J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation yun-2007-4 The novel cellular mechanism of human 5-HT6 receptor through an interaction with Fyn. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yun HM., Kim S., Kim HJ., Kostenis E., Kim JI., Seong JY., Baik JH., Rhim H. hrhim@kist.re.kr Human brain cDNA J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation yun-2007-5 The novel cellular mechanism of human 5-HT6 receptor through an interaction with Fyn. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yun HM., Kim S., Kim HJ., Kostenis E., Kim JI., Seong JY., Baik JH., Rhim H. hrhim@kist.re.kr Human brain cDNA J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation yun-2007-7 The novel cellular mechanism of human 5-HT6 receptor through an interaction with Fyn. crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yun HM., Kim S., Kim HJ., Kostenis E., Kim JI., Seong JY., Baik JH., Rhim H. hrhim@kist.re.kr Human brain cDNA J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation 5ht6r_human 5-hydroxytryptamine receptor 6 Serotonin receptor 6 HTR6 protein protein human Homo sapiens Human MVPEPGPTANSTPAWGAGPPSAPGGSGWVAAALCVVIALTAAANSLLIALICTQPALRNTSNFFLVSLFTSDLMVGLVVMPPAMLNALYGRWVLARGLCLLWTAFDVMCCSASILNLCLISLDRYLLILSPLRYKLRMTPLRALALVLGAWSLAALASFLPLLLGWHELGHARPPVPGQCRLLASLPFVLVASGLTFFLPSGAICFTYCRILLAARKQAVQVASLTTGMASQASETLQVPRTPRPGVESADSRRLATKHSRKALKASLTLGILLGMFFVTWLPFFVANIVQAVCDCISPGLFDVLTWLGYCNSTMNPIIYPLFMRDFKRALGRFLPCPRCPRERQASLASPSLRTSHSGPRPGLSLQQVLPLPLPPDSDSDSDAGSGGSSGLRLTAQLLLPGEATQDPPLPTRAAAAVNFFNIDPAEPELRPHPLGIPTN fyn_human Tyrosine-protein kinase Fyn p59-Fyn Proto-oncogene c-Fyn Src-like kinase FYN Proto-oncogene Syn protein protein human Homo sapiens Human MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL g3hmr9_crigr I79_012041 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQGFLEDYFTATEPQYQPGENL fyn-htr6-6 Interaction between HTR6 and FYN identified by coimmunoprecipitation 575371 N/A 575378 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 575379 unspecified role unspecified role prey prey physical association physical association false false false 2D fyn-htr6-8 Interaction between HTR6 and FYN identified by coimmunoprecipitation 575371 N/A 575380 unspecified role unspecified role prey prey N/A 575378 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2D fyn-htr6-1 Interaction between HTR6 and FYN identified by Y2H 575372 N/A 575378 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 575379 unspecified role unspecified role prey prey physical association physical association false false false fyn-htr6-2 Interaction between HTR6 and FYN identified by Y2H 575373 N/A 575379 unspecified role unspecified role prey prey N/A 575378 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 fyn-htr6-3 Interaction between HTR6 and FYN identified by pulldown 575374 N/A 575379 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sh3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 575378 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 575402 575400 direct interaction direct interaction false false false 2B fyn-htr6-4 Interaction between HTR6 and FYN identified by coimmunoprecipitation 575375 N/A 575379 unspecified role unspecified role prey prey N/A 575378 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2C fyn-htr6-5 Interaction between HTR6 and FYN identified by coimmunoprecipitation 575376 N/A 575379 unspecified role unspecified role bait bait N/A 575378 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2C fyn-htr6-7 Interaction between HTR6 and FYN identified by coimmunoprecipitation 575377 N/A 575378 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 575379 unspecified role unspecified role bait bait physical association physical association false false false 2C