IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hedman-2006-1 Proteomic identification of glucocorticoid receptor interacting proteins. rat-liver rattus norvegicus liver Rat liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se Proteomics (1615-9853) 2006 Only protein-protein interactions 5 imex curation hedman-2006-2 Proteomic identification of glucocorticoid receptor interacting proteins. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se Proteomics (1615-9853) 2006 Only protein-protein interactions 5 imex curation hedman-2006-3 Proteomic identification of glucocorticoid receptor interacting proteins. rat-htc Rat hepatoma HTC cells htc Rat hepatoma HTC cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se Proteomics (1615-9853) 2006 Only protein-protein interactions 5 imex curation hedman-2006-4 Proteomic identification of glucocorticoid receptor interacting proteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se Proteomics (1615-9853) 2006 Only protein-protein interactions 5 imex curation hedman-2006-5 Proteomic identification of glucocorticoid receptor interacting proteins. rat-liver rattus norvegicus liver Rat liver liver bn-page blue native page bn-page ms participant Identification by mass spectrometry ms participant Hedman E., Widen C., Asadi A., Dinnetz I., Schroder WP., Gustafsson JA., Wikstrom AC. Apoptosis - Interactions involving proteins with a function related to apoptosis A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 2), CAA49670 and Q91YP3 ( a mouse protein) Both ligand activated and non-ligand activated samples were used in this study. Liganded state of the glucocorticoid recepter (GR) was prepared by adding 20nM triamcinolone acetonide to the cytosol at 47C for 1 h. Activation was later accomplished by dilution and heat-activation at 25C for 30 min.Protein spots were collected from a total of ten gels. All proteins that matched fingerprint spectra with a MOWSE score above 90 were considered significant. Proteins were mapped by using sequence similarity search of protein sequences identified by given identifiers within the paper against the UniProt database using the MPsrch tool. A number of proteins could not be assigned UniProt accession numbers, these were (as appear in Table 1) 1NLD_L (identifies a mouse protein), ATRTC, CAA49670, XP_227551 and AAH86531. erik.hedman@mednut.ki.se Proteomics (1615-9853) 2006 Only protein-protein interactions 5 imex curation mvp_rat Major vault protein Mvp protein protein rat Rattus norvegicus (Rat) Rat MATEEAIIRIPPYHYIHVLDQNSNVSRVEVGPKTYIRQDNERVLFAPVRMVTVPPRHYCIVANPVSRDTQSSVLFDITGQVRLRHADQEIRLAQDPFPLYPGEVLEKDITPLQVVLPNTALHLKALLDFEDKNGDKVMAGDEWLFEGPGTYIPQKEVEVVEIIQATVIKQNQALRLRARKECFDREGKGRVTGEEWLVRSVGAYLPAVFEEVLDLVDAVILTEKTALHLRALQNFRDLRGVLHRTGEEWLVTVQDTEAHVPDVYEEVLGVVPITTLGPRHYCVILDPMGPDGKNQLGQKRVVKGEKSFFLQPGERLERGIQDVYVLSEQQGLLLKALQPLEEGESEEKVSHQAGDCWLIRGPLEYVPSAKVEVVEERQAIPLDQNEGIYVQDVKTGKVRAVIGSTYMLTQDEVLWEKELPSGVEELLNLGHDPLADRGQKGTAKPLQPSAPRNKTRVVSYRVPHNAAVQVYDYRAKRARVVFGPELVTLDPEEQFTVLSLSAGRPKRPHARRALCLLLGPDFFTDVITIETADHARLQLQLAYNWHFELKNRNDPAEAAKLFSVPDFVGDACKAIASRVRGAVASVTFDDFHKNSARIIRMAVFGFEMSEDTGPDGTLLPKARDQAVFPQNGLVVSSVDVQSVEPVDQRTRDALQRSVQLAIEITTNSQEAAAKHEAQRLEQEARGRLERQKILDQSEAEKARKELLELEAMSMAVESTGNAKAEAESRAEAARIEGEGSVLQAKLKAQALAIETEAELERVKKVREMELIYARAQLELEVSKAQQLANVEAKKFKEMTEALGPGTIRDLAVAGPEMQVKLLQSLGLKSTLITDGSSPINLFSTAFGLLGLGSDGQPPAQK q7tqn4_rat nos2 rCG_48632 Rela RelA protein protein rat Rattus norvegicus (Rat) Rat MDDLFPLIFPSEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLTPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFNGPTEPRPPPRRIAVPSRGPTSVPKPAPQPYAFSTSLSTINFDEFSPMVLPPGQISNQALALAPSSAPVLAQTMVPSSAMVPSLAQPPAPVPVLAPGPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNNTDPGVFTDLASVDNSEFQQLLNQGVAMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPATLGTSGLPNGLSGDEDFSSIADMDFSALLSQISS hs90b_rat Heat shock protein HSP 90-beta Heat shock 84 kDa Hsp90ab1 Hsp84 Hspcb protein protein rat Rattus norvegicus (Rat) Rat MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKEDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVTAEEPSAAVPDEIPPLEGDEDASRMEEVD ttpa_rat Alpha-tocopherol transfer protein Ttpa Tpp1 protein protein rat Rattus norvegicus (Rat) Rat MAEMRPGPVVGKQLNEQPDHSPLVQPGLAELRRRAQEEGVPETPQPLTDAFLLRFLRARDFDLDLAWRLMKNYYKWRAECPELSADLHPRSILGLLKAGYHGVLRSRDPTGSRVLIYRISYWDPKVFTAYDVFRVSLITSELIVQEVETQRNGVKAIFDLEGWQISHAFQITPSVAKKIAAVVTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKGRIHLHGNNYKSSLLQHFPDILPLEYGGNESSMEDICQEWTNFIMKSEDYLSSISETIQ gcr_rat Glucocorticoid receptor Nuclear receptor subfamily 3 group C member 1 Nr3c1 Grl protein protein rat Rattus norvegicus (Rat) Rat MDSKESLAPPGRDEVPGSLLGQGRGSVMDFYKSLRGGATVKVSASSPSVAAASQADSKQQRILLDFSKGSTSNVQQRQQQQQQQQQQQQQQQQQQQPDLSKAVSLSMGLYMGETETKVMGNDLGYPQQGQLGLSSGETDFRLLEESIANLNRSTSVPENPKSSTSATGCATPTEKEFPKTHSDASSEQQNRKSQTGTNGGSVKLYPTDQSTFDLLKDLEFSAGSPSKDTNESPWRSDLLIDENLLSPLAGEDDPFLLEGNTNEDCKPLILPDTKPKIKDTGDTILSSPSSVALPQVKTEKDDFIELCTPGVIKQEKLGPVYCQASFSGTNIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPVFNVIPPIPVGSENWNRCQGSGEDSLTSLGALNFPGRSVFSNGYSSPGMRPDVSSPPSSSSAATGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAILGLRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEYLCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK ppp6_rat Serine/threonine-protein phosphatase 6 catalytic subunit Protein phosphatase V Ppp6c Ppv protein protein rat Rattus norvegicus (Rat) Rat MAPLDLDKYVEIARQCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQVPDTNYIFMGDFVDRGYYSLETFTYLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWRYCTKVFDMLTVAALIDEQILCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPRGAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNTREPKLFRAVPDSERVIPPRTTTPYFL albu_rat Albumin Alb protein protein rat Rattus norvegicus (Rat) Rat MKWVTFLLLLFISGSAFSRGVFRREAHKSEIAHRFKDLGEQHFKGLVLIAFSQYLQKCPYEEHIKLVQEVTDFAKTCVADENAENCDKSIHTLFGDKLCAIPKLRDNYGELADCCAKQEPERNECFLQHKDDNPNLPPFQRPEAEAMCTSFQENPTSFLGHYLHEVARRHPYFYAPELLYYAEKYNEVLTQCCTESDKAACLTPKLDAVKEKALVAAVRQRMKCSSMQRFGERAFKAWAVARMSQRFPNAEFAEITKLATDVTKINKECCHGDLLECADDRAELAKYMCENQATISSKLQACCDKPVLQKSQCLAEIEHDNIPADLPSIAADFVEDKEVCKNYAEAKDVFLGTFLYEYSRRHPDYSVSLLLRLAKKYEATLEKCCAEGDPPACYGTVLAEFQPLVEEPKNLVKTNCELYEKLGEYGFQNAVLVRYTQKAPQVSTPTLVEAARNLGRVGTKCCTLPEAQRLPCVEDYLSAILNRLCVLHEKTPVSEKVTKCCSGSLVERRPCFSALTVDETYVPKEFKAETFTFHSDICTLPDKEKQIKKQTALAELVKHKPKATEDQLKTVMGDFAQFVDKCCKAADKDNCFATEGPNLVARSKEALA lasp1_rat LIM and SH3 domain protein 1 Lasp1 protein protein rat Rattus norvegicus (Rat) Rat MNPNCARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHYPKQSFTMVADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVADTPELQRIKKTQDQISNIKYHEEFEKSRMGPSGGEGIEPERREAQDSSSYRRPTEQQQPQPHHIPTSAPVYQQPQQQQVTPSYGGYKEPAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYGTVERTGDTGMLPANYVEAI fetua_rat Alpha-2-HS-glycoprotein Fetuin-A Glycoprotein PP63 59 kDa bone sialic acid-containing protein Ahsg Fetua protein protein rat Rattus norvegicus (Rat) Rat MKSLVLLLCFAQLWSCQSAPQGAGLGFRELACDDPETEHVALIAVDYLNKHLLQGFRQILNQIDKVKVWSRRPFGEVYELEIDTLETTCHALDPTPLANCSVRQQAEHAVEGDCDFHILKQDGQFRVLHAQCHSTPDSAEDVRKFCPRCPILIRFNDTNVVHTVKTALAAFNAQNNGTYFKLVEISRAQNVPFPVSTLVEFVIAATDCTGQEVTDPAKCNLLAEKQYGFCKATLIHRLGGEEVSVACKLFQTQPQPANANPAGPAPTVGQAAPVAPPAGPPESVVVGPVAVPLGLPDHRTHHDLRHAFSPVASVESASGEVLHSPKVGQPGDAGAAGPVAPLCPGRVRYFKI prdx1_rat Peroxiredoxin-1 Thioredoxin peroxidase 2 Thioredoxin-dependent peroxide reductase 2 Heme-binding 23 kDa protein HBP23 Prdx1 Tdpx2 Thioredoxin-dependent peroxiredoxin 1 protein protein rat Rattus norvegicus (Rat) Rat MSSGNAKIGHPAPSFKATAVMPDGQFKDISLSDYKGKYVVFFFYPLDFTFVCPTEIIAFSDRAEEFKKLNCQVIGASVDSHFCHLAWINTPKKQGGLGPMNIPLVSDPKRTIAQDYGVLKADEGISFRGLFIIDDKGILRQITINDLPVGRSVDEILRLVQAFQFTDKHGEVCPAGWKPGSDTIKPDVNKSKEYFSKQK dnja2_rat DnaJ homolog subfamily A member 2 RDJ2 Dnaja2 protein protein rat Rattus norvegicus (Rat) Rat MANVADTKLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGEVINEKDRCKKCEGKKVIKEVKILEVHVDKGMKHGQRITFTGEADQAPGVEPGDIVLFVQEKEHEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDARQIVVKYPPGKVIEPGCVRVVRGEGMPQYRNPFEKGDLYIKFDVQFPENNWINPDKLSELEDLLPSRPEVPNVIGETEEVELQEFDSTRGSGGGQRREAYNDSSDEESSSHHGPGVQCAHQ home2_rat Homer protein homolog 2 Cupidin VASP/Ena-related gene up-regulated during seizure and LTP 2 Homer2 Vesl2 protein protein rat Rattus norvegicus (Rat) Rat MGEQPIFTTRAHVFQIDPSTKKNWVPASKQAVTVSYFYDVTRNSYRIISVDGAKVIINSTITPNMTFTKTSQKFGQWADSRANTVFGLGFSSEQQLTKFAEKFQEVREAARLARDKSQEKIETSSNHSQESGCETPSSTQASSVNGTDDEKASHASPADTHLKSENDKLKIALTQSAANVKKWEIELQTLRESNARLTTALQESAASVEQWKRQFSICRDENDRLRSKIEELEEQCGEINREKEKNTQLKRRIEELESEVREKEMELKDLRKQSEIIPQLMSECEYVSEKLEAAERDNQNLEDKVRSLKTDIEESKYRQRHLKGELKSFLEVLDGKIDDLHDFRRGLSKLGTDN pawr_rat PRKC apoptosis WT1 regulator protein Prostate apoptosis response 4 protein Transcriptional repressor Par-4-like protein PAWR Pawr Par4 protein protein rat Rattus norvegicus (Rat) Rat MATGGYRSSGSTTDFLEEWKAKREKMRAKQNPVGPGSSGGDPAAKSPAGPLAQTTAAGTSELNHGPAGAAAPAAPGPGALNCAHGSSALPRGAPGSRRPEDECPIAAGAAGAPASRGDEEEPDSAPEKGRSSGPSARKGKGQIEKRKLREKRRSTGVVNIPAAECLDEYEDDEAGQKERKREDAITQQNTIQNEAASLPDPGTSYLPQDPSRTVPGRYKSTISAPEEEILNRYPRTDRSGFSRHNRDTSAPANFASSSTLEKRIEDLEKEVLRERQENLRLTRLMQDKEEMIGKLKEEIDLLNRDLDDMEDENEQLKQENKTLLKVVGQLTR a16a1_rat Aldehyde dehydrogenase family 16 member A1 Aldh16a1 protein protein rat Rattus norvegicus (Rat) Rat MAATRIQPSTREIFTTLEYGPVPESHACALAWLDTHNRLLGHYVNGMWLKPEHRNPAPCQDPITGENLASCLQAEAEDISAAVEAARITFKTWSQLPGTARGQHLTRLAKVIQKHQRLLWTLESLVTGRAVREVRDGDIPLAQQLLHYHALQAHAQDSALADWEPLGVIGLILPTSFSFLDMMWRVCPALAMGCTVVALVPPSFPTPLLLAQLAGELGSFPGILNVVCGPVSLGPVLASQPGVQKVAFCGAVEEGRALRQTLAGRGATLGLALGTESLLLLTDSADVDSAVEGVVDAVWSDRSLGGLRLLIQESVWDEAMKRLQARMAQIRSGKGLDGAVDMGARGAAARDLAQSFVDEAQSQGGQVFQAGDMPSNSPFFSPTLVSGLPPAAPCAQAEVPWPVVMASPFRTVKEALALANGTPRGGSASVWSERLGQALELGYGLQVGTVWINAHGLRDPAVPTGGCKESGSSWHGGPDGLYEYLQLLGTPYRESFLCESLNYDTFGLAVSSSLPSGPETGPSPAPPYGLFVRGRFQSPGTQSSRPIKDSSGKVSSYVAEGGAKDIRGAVEAAHQAAPGWGAQSPRARASLLWALAAALERRKQVLAAQLERHGAAPTVAETEVELSVRRLQTWATRVQDQGQTLQVTGLRGPVLRLREPLGVLAVVCPDEWPMLAFVSLLAPALAHGNAVVLVPSGSCPLLALEACQDIAALFPAGLVNVVTGDRDHLTRCLALHQDVQAMWYFGSAQGSQFVEWASAGNLKPVWVNRDFPRAWDVEVQGSEQELSLHAARTKALWLPMGD tbb2b_rat Tubulin beta-2B chain T beta-15 Tubb2b Tubb2a protein protein rat Rattus norvegicus (Rat) Rat MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEEGEDEA fibb_rat Fibrinogen beta chain Liver regeneration-related protein LRRG036/LRRG043/LRRG189 Fgb Ab1-181 Ab1-216 Ac1-581 protein protein rat Rattus norvegicus (Rat) Rat MRHLWLLLLSVSLVQTQAATTDSDKVDLSIARGHRPVDRRKEEPPSLRPAPPPISGGGYRARPAKVDAGQKKVERKPPDAGGCVHGDGDMGVLCPTGCELRQTLLNHERPIKNSIAELNSNINSVSETSSVTFQYLTLLKDMWKKKQAQVKDNENVINEYSSILEDQKLYIDETVNDNIPLNLRVLRSILEDLRSKIQKLESDISAQTEYCHTPCTVNCNIPVVSGKECEEIIRKGGETSEMYLIQPDTSSKPYRVYCDMKTENGGWTVIQNRQDGSVDFGRKWDPYKKGFGNIATNEDTKKYCGLPGEYWLGNDKISQLTRIGPTELLIEMEDWKGDKVKAHYGGFTVQTEANKYQVSVNKYKGTAGNALMEGASQLVGENRTMTIHNGMFFSTYDRDNDGWVTTDPRKQCSKEDGGGWWYNRCHAANPNGRYYWGGLYSWDMSKHGTDDGVVWMNWKGSWYSMRRMSMKIRPVFPQQ elob_rat Elongin-B Elob Elongin 18 kDa subunit RNA polymerase II transcription factor SIII subunit B SIII p18 Tceb2 Transcription elongation factor B polypeptide 2 protein protein rat Rattus norvegicus (Rat) Rat MDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPEEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALRIEPFSSPPELPDVMKPQDSGGSANEQAVQ eloc_rat Elongin-C Eloc Elongin 15 kDa subunit RNA polymerase II transcription factor SIII subunit C SIII p15 Stromal membrane-associated protein SMAP1B homolog Tceb1 Transcription elongation factor B polypeptide 1 protein protein rat Rattus norvegicus (Rat) Rat MDGEEKTYGGCEGPDAMYVKLISSDGHEFIVKREHALTSGTIKAMLSGPGQFAENETNEVNFREIPSHVLSKVCMYFTYKVRYTNSSTEIPEFPIAPEIALELLMAANFLDC mp2k2_rat Dual specificity mitogen-activated protein kinase kinase 2 ERK activator kinase 2 MAPK/ERK kinase 2 Map2k2 Mek2 Mkk2 Prkmk2 protein protein rat Rattus norvegicus (Rat) Rat MLARRKPVLPALTINPTIAEGPSPTSEGASEAHLVDLQKKLEELDLDEQQRKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKARHRPSGLIMARKLIHLEIKPAVRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEDILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVELAIGRYPIPPPDAKELEASFGRPVVDGADGEPHSVSPRPRPPGRPISGHGMDSRPAMAIFELLDYIVNEPPPKLPSGVFSSDFQEFVNKCLIKNPAERADLKLLTNHAFIKRSEGEDVDFAGWLCRTLRLKQPSTPTRTAV bhmt1_rat Betaine--homocysteine S-methyltransferase 1 Bhmt protein protein rat Rattus norvegicus (Rat) Rat MAPIAGKKAKRGILERLNAGEVVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYASEDKLENRGNYVAEKISGQKVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKIFHQQLEVFMKKNVDFLIAEYFEHVEEAVWAVEALKTSGKPIAATMCIGPEGDLHGVSPGECAVRLVKAGAAIVGVNCHFDPSTSLQTIKLMKEGLEAARLKAYLMSHALAYHTPDCGKQGFIDLPEFPFGLEPRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARKEYWQNLRIASGRPYNPSMSKPDAWGVTKGAAELMQQKEATTEQQLRALFEKQKFKSAQ fabpl_rat Fatty acid-binding protein, liver Fabp1 Liver-type fatty acid-binding protein Squalene- and sterol-carrier protein Z-protein p14 Fatty acid-binding protein 1 protein protein rat Rattus norvegicus (Rat) Rat MNFSGKYQVQSQENFEPFMKAMGLPEDLIQKGKDIKGVSEIVHEGKKVKLTITYGSKVIHNEFTLGEECELETMTGEKVKAVVKMEGDNKMVTTFKGIKSVTEFNGDTITNTMTLGDIVYKRVSKRI tba1a_rat Tubulin alpha-1A chain Tuba1a Tuba1 Tubulin alpha-1 chain Alpha-tubulin 1 protein protein rat Rattus norvegicus (Rat) Rat MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY hba_rat Hemoglobin subunit alpha-1/2 Hba1 Hba-a1 Hemoglobin alpha-1/2 chain Alpha-1/2-globin protein protein rat Rattus norvegicus (Rat) Rat MVLSADDKTNIKNCWGKIGGHGGEYGEEALQRMFAAFPTTKTYFSHIDVSPGSAQVKAHGKKVADALAKAADHVEDLPGALSTLSDLHAHKLRVDPVNFKFLSHCLLVTLACHHPGDFTPAMHASLDKFLASVSTVLTSKYR dnja1_rat DnaJ homolog subfamily A member 1 DnaJ-like protein 1 Dnaja1 Hsj2 Rdj1 Heat shock protein J2 protein protein rat Rattus norvegicus (Rat) Rat MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLADSKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS gcr_human Glucocorticoid receptor Nuclear receptor subfamily 3 group C member 1 NR3C1 GRL protein protein human Homo sapiens Human MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDVSSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDENCLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKEDFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPIFNVIPPIPVGSENWNRCQGSGDDNLTSLGTLNFPGRTVFSNGYSSPSMRPDVSSPPSSSSTATTGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATTGVSQETSENPGNKTIVPATLPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSTWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSANLLCFAPDLIINEQRMTLPCMYDQCKHMLYVSSELHRLQVSYEEYLCMKTLLLLSSVPKDGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLNYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK hprt_rat Hypoxanthine-guanine phosphoribosyltransferase Hprt1 Hprt protein protein rat Rattus norvegicus (Rat) Rat MSTLSPSVVISDDEPGYDLDLFCIPNHYAEDLEKVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVKQYSPKMVKVASLLVKRTSRSVGYRPDFVGFEIPDKFVVGYALDYNEHFRDLNHVCVISESGKAKYKA skp1_rat S-phase kinase-associated protein 1 Skp1 Skp1a S-phase kinase-associated protein 1A Cyclin-A/CDK2-associated protein p19 p19skp1 p19A protein protein rat Rattus norvegicus (Rat) Rat MPTIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK alb-dnaja2-gr-hgprt 577313 N/A 577318 unspecified role unspecified role prey prey N/A 577319 unspecified role unspecified role prey prey N/A 577320 unspecified role unspecified role prey prey N/A 577321 unspecified role unspecified role prey prey N/A 577322 unspecified role unspecified role bait bait N/A 577323 unspecified role unspecified role prey prey N/A 577324 unspecified role unspecified role prey prey N/A 577325 unspecified role unspecified role prey prey N/A 577326 unspecified role unspecified role prey prey N/A 577327 unspecified role unspecified role prey prey N/A 577328 unspecified role unspecified role prey prey N/A 577329 unspecified role unspecified role prey prey N/A 577330 unspecified role unspecified role prey prey N/A 577331 unspecified role unspecified role prey prey N/A 577332 unspecified role unspecified role prey prey N/A 577333 unspecified role unspecified role prey prey N/A 577334 unspecified role unspecified role prey prey N/A 577335 unspecified role unspecified role prey prey N/A 577336 unspecified role unspecified role prey prey N/A 577337 unspecified role unspecified role prey prey N/A 577338 unspecified role unspecified role prey prey N/A 577339 unspecified role unspecified role prey prey N/A 577340 unspecified role unspecified role prey prey N/A 577341 unspecified role unspecified role prey prey association association false false false Table 1. Table 2. The following proteins were reported as having avarage ratios (non-liganded(nn-activated) over liganded (activated))of standardised spot volumes. P24090 (ratio = 5.84), Q6AYZ1(ratio = 4.17), P06399 (four spots ratio = 4.83, 3.88, 6.37,7.09 ), P63037 (ratio = 11.29), P14480 (4.28), P13832 (two spots ratio = -21.44, -13.40), P62870 (ratio = 5.95) and P27605 (ratio = -13.38) gr-mvp 577314 N/A 577342 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 577318 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2a gr-mvp-1 577315 N/A 577322 unspecified role unspecified role bait bait N/A 577318 unspecified role unspecified role prey prey physical association physical association false false false 2b gr-mvp-2 577316 N/A 577342 unspecified role unspecified role prey prey N/A 577318 unspecified role unspecified role bait bait physical association physical association false false false 2c gp-hgprt-skip1a 577317 N/A 577320 unspecified role unspecified role neutral component neutral component N/A 577331 unspecified role unspecified role neutral component neutral component N/A 577324 unspecified role unspecified role neutral component neutral component N/A 577343 unspecified role unspecified role neutral component neutral component N/A 577344 unspecified role unspecified role neutral component neutral component N/A 577322 unspecified role unspecified role neutral component neutral component N/A 577337 unspecified role unspecified role neutral component neutral component physical association physical association false false false Table 2 The authors provide a list of proteins isolated from several separate protein complexes and do not differentiate which proteins were identified in a specific complex. This entry lists the proteins from several protein complexes as set out in Table 2 of the report. Therefore the user should be aware that this entry does not indicate that all of the proteins were found in a single protein complex. The participant EBI-1187287(O09171) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later.