UniProt UniProt http://www.uniprot.org kao-2006-1 Schizosaccharomyces pombe Git1 is a C2-domain protein required for glucose activation of adenylate cyclase. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kao RS., Morreale E., Wang L., Ivey FD., Hoffman CS. hoffmacs@bc.edu Genetics (0016-6731) 2006 Only protein-protein interactions imex curation kao-2006-2 Schizosaccharomyces pombe Git1 is a C2-domain protein required for glucose activation of adenylate cyclase. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast confocal microscopy confocal microscopy predetermined predetermined participant predetermined Kao RS., Morreale E., Wang L., Ivey FD., Hoffman CS. hoffmacs@bc.edu Genetics (0016-6731) 2006 Only protein-protein interactions imex curation git1_schpo Adenylate cyclase activation protein git1 git1 SPBC21C3.20c Glucose-insensitive transcription protein 1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGFTSVEPPDFFDWTARAIFLLSTLRSRRNAKQIERENALLDQIKELLSIAIIQCHERNIPSIDLPPSFFNYVCEQLPRHPFAKIASSYIDTHTLFFQISISSFLCRFSNCSEDEIRLFKQHQSLDSFLLSLFDLLCSHHFSVEFTSISLIIKSSQWLFYTLSDYAHESLTGVEADITTKFLSTLADTWPEFEEQCTLSKHLFLLGDFSLISYVKVLCGVFNIPNYNAFILSVEDLLPENISFAAVADFQLSKYRAINDNSVLRSDDFSSKAVFDKYVERECYYSENAISKIFQNYPDLAVLFEELQYAEGNNDLLSAILNTNKYTLRPDDCAPYFYRLMDLCLRYCYSQSNEIGTNVLWSMTTSFINIWRIHPGTCCCVVLSIYWPYLEDKLLKFSDLGDIFEYFFSLIHDCDYAIWSVHDEHQFSELLAFYHRRALNDLVEELGSCPRSSDSCRAILDILNNYIFSCPLFLVTPEELEEQYTDFFKKLKEKYGEFFQNLLKKELTGKPESDLEGLRLVGVQLQATYENLKSNFSARIFNQLSVHSFFAEATCHLFLEKLPTITKTYVYSNCKEIPDHILEMINALYQQTRALVACTGYLSSDYPKIYEAFSGTFVYRNLSLIIDKGLANVLQTVLRESFIPKRSRFYSDSTKMILAPLQDCYDFLQKIDWPSSKDANRFYLHLGKLSENYIHYYVDSMYALFIYSTDIPLKAEPLVPDLPVKQLKEKASHFKRHSGDPNFLKLYRESFTLINTVSLVSGWLKMLHQRIDYKILTEKVMHFKDMEVPSATVIISIKMAHLLVTPKAPYSFSVLLQDPESEFCVHTRDVYDELVPNWKETYQMEITNVKRFKLIIYQRNIHTGLETVYGHATLEVDPDKLKQGHGKDVWLDITNKGQILIGLRVLLKRETPNYYCFAQMEYLTVTEVKMLNVLMISLSKFIRNSLRNSGLMDFVRYLDGDENDNAEAETKEKMDELQARCEKEQQSLLAELDDFFYWIYVNLLPKPLFYLLSRLWFGLLETFRDILVPPVTDSTLRRIPLNKRELKMVYSWLQLFYDFFRNFQDIAPFDFLNTDEYKSVMAIKDYYFVKSKDVKRVSS cyaa_schpo Adenylate cyclase SPBC19C7.03 cyr1 ATP pyrophosphate-lyase Adenylyl cyclase protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDQSKRLLKSAVPNPPEHFKTGISWLDDLDEKDDDSATSVNYDIPEITEANLCNDSHEALSPCTQPVGNSGRPVEAFKTYPSTPAVPSKSVLFHFYEPDENFSLSDTGRTKSDTALAARESSEKSEVPRDTRSAGIKPYKENNSSNCAISKEAGLRRLIDKDRESFDKNLNQSFTNLTFPEPISDDSDSVEFQRDSLNNNWPASLEGSIHELPRNSDDDGIPASAAHILDLDYHRDSYDSPWKKFLPYPSILSDDSWKAPESWGTSLPTEAIPKQVFTTRFFARPSLGNRKKEFFLRVYRDDRTSVSFICPIGIQTHEVIKLLARLFFLPSSANFYLLLIQFNTERILLPHEQPCIIFERLLSLFGCKVTSDEEINEEDNYSVARLVFTTMDIGADVLRKFSEKKITANLDISRSNLEVIPVKIYPYAHELISLNVSHNLSLDLPLDFMERCVKLKRLDISNNLRSPRGKPITALRQLEVLNMSRNDIYELDPLIFSGLSRNSLKELNIANNKLFFLPHSTRYLVNLTYLDLSYNNFVTFPLIITELSQLETLNFSHNLLSQISSKIGSLVKLKHLYLQFNDLSNRLPQEIGLLKNLETIDLSYNAITNIASLSECPKLNSINVACNLLSFYEYSNPSATFIDFSFCPLTTIDPAFSYSNLVYFDISHAKLIGLKDSVIETLVNVETVKVNYNHFTSISDAISAMQNLKYLSCTNCEMSYVSPNLGKLKHLVHLDLHANNIKIFPEEVWQVSSLKVVNLSSNILEKIKLPVATSKKLTRTISQLKIMRTLSGNPVSSLSSQEFVMPTVEELYLVDNRLGNDCFTALEYFKCLKVLNLSYNYLTEIPSKFFQNFSDLKHLFVSGNELANLSISSTAQVLLETLYANGNRLSSFPKNEALSKSLRFLDISTNNLQNLAVEKAEKKSLTKLPQLEYLNLSGNTWFRFSEHEDTNFTKSYLKNLKFLSIMDLNTKFSNAPSDVLNHFIQRNSPQPNILRYGVCGYLSRSIPVISACELVVNNFLHPQSSLYCVLDSDISAGKNNRVLKFVYDNLASCLAHEINAADSSSEQICNALRRGFLRLNKKLGNVIHYDLRKSSEGDVDSNYVTTMNISEKGYSMDSSCLDIGVSIILVYVRDTRAFVANVGTSMAIMSTRNDSEPTTLSVMHDVYNRDEIRRIVDSCGFISGEIKSTTTRAIGRLSQFPGVQAVPYVNVQYLSELNEFIILANQEFWSVLSKRTVIDVVRANRHSPLLASTKLRDYAIAYGAEKNVLVVIVELNGLFEENSLNFNQLRGDEKTLAISEKNDNMSFVQDLPDDSSLARMNREVSPPKGCIAMVFTDIKNSTLLWERHPIAMRSAIKTHNTIMRRQLRATGGYEVKTEGDAFMVCFQTVPAALLWCFSVQLQLLSADWPNEIVESVQGRLVLGSKNEVLYRGLSVRIGVNYGVTVSELDPITRRMDYYGPVVNRTSRVVSVADGGQIAVSAEVVSVLNQLDSETMSSEKTNVNEMEVRALKQIGYIIHNLGEFKLKGLDTTEMISLVYPVQLQGRLERLIKSRSLGTPTALPETQTYTPVRSRSNSLRPMLARLSDSKSVHGEEGGSGKRSVSSLRNVSPSESTGGYEGCIFDDQQYQLLYELCERLEDHAAILHGFPEPPPCDTGLAAPVNQAEEYSLFYRLTLRIENTIYCVSQMLGHTG git1-git2 671451 N/A 671453 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671454 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 8A git1-git2-1 671451 N/A 671453 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671454 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 8B git1-git2-2 671452 N/A 671453 unspecified role unspecified role neutral component neutral component region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671454 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 9B