UniProt UniProt http://www.uniprot.org chasse-2006-1 Genome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Chasse SA., Flanary P., Parnell SC., Hao N., Cha JY., Siderovski DP., Dohlman HG. hdohlman@med.unc.edu Eukaryotic Cell 5 (330-346) 2006 sst2_yeast Protein SST2 SST2 L9324.9 YLR452C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVDKNRTLHELSSKNFSRTPNGLIFTNDLKTVYSIFLICLDLKEKKHSSDTKSFLLTAFTKHFHFTFTYQEAIKAMGQLELKVDMNTTCINVSYNIKPSLARHLLTLFMSSKLLHTPQDRTRGEPKEKVLFQPTPKGVAVLQKYVRDIGLKTMPDILLSSFNSMKLFTFERSSVTDSIIHSDYLIHILFIKMMGAKPNVWSPTNADDPLPCLSSLLEYTNNDDTFTFEKSKPEQGWQAQIGNIDINDLERVSPLAHRFFTNPDSESHTQYYVSNAGIRLFENKTFGTSKKIVIKYTFTTKAIWQWIMDCTDIMHVKEAVSLAALFLKTGLIVPVLLQPSRTDKKKFQISRSSFFTLSKRGWDLVSWTGCKSNNIRAPNGSTIDLDFTLRGHMTVRDEKKTLDDSEGFSQDMLISSSNLNKLDYVLTDPGMRYLFRRHLEKELCVENLDVFIEIKRFLKKMTILKKLIDSKHCDKKSNTSTSKNNIVKTIDSALMKQANECLEMAYHIYSSYIMIGSPYQLNIHHNLRQNISDIMLHPHSPLSEHFPTNLYDPSPASAESAASSISSTEADTLGEPPEVSLKPSKNLSNENCSFKKQGFKHQLKEYKPAPLTLAETHSPNASVENSHTIVRYGMDNTQNDTKSVESFPATLKVLRKLYPLFEIVSNEMYRLMNNDSFQKFTQSDVYKDASALIEIQEKC gpa1_yeast Guanine nucleotide-binding protein alpha-1 subunit YHR005C GPA1 CDC70 DAC1 SCG1 GP1-alpha protein protein yeast Saccharomyces cerevisiae Baker's yeast MGCTVSTQTIGDESDPFLQNKRANDVIEQSLQLEKQRDKNEIKLLLLGAGESGKSTVLKQLKLLHQGGFSHQERLQYAQVIWADAIQSMKILIIQARKLGIQLDCDDPINNKDLFACKRILLKAKALDYINASVAGGSDFLNDYVLKYSERYETRRRVQSTGRAKAAFDEDGNISNVKSDTDRDAETVTQNEDADRNNSSRINLQDICKDLNQEGDDQMFVRKTSREIQGQNRRNLIHEDIAKAIKQLWNNDKGIKQCFARSNEFQLEGSAAYYFDNIEKFASPNYVCTDEDILKGRIKTTGITETEFNIGSSKFKVLDAGGQRSERKKWIHCFEGITAVLFVLAMSEYDQMLFEDERVNRMHESIMLFDTLLNSKWFKDTPFILFLNKIDLFEEKVKSMPIRKYFPDYQGRVGDAEAGLKYFEKIFLSLNKTNKPIYVKRTCATDTQTMKFVLSAVTDLIIQQNLKKIGII 2E87C546E133D6E5 rgs2_yeast Regulator of G-protein signaling 2 RGS2 YOR3224w YOR107W protein protein yeast Saccharomyces cerevisiae Baker's yeast MASVPSLCDILIPLEKNSRSDGDAESSNTVLIQLRKGHHERMRSPYTIQKFYKFLKRAHCEENLEFFEKAHQFLQLKQNRSISEEKLLEVWNKSLYIKYIAVDSPKECNFSQDTREIFEKCFANNEVPADVDVLCAISHVMGLLMDGYHRFVSSVNEKKYSATYAHNDSATEQDLKNESTTSFSSLGVEDISEDRNSYLKKPDINGLSTIIQETSANTTNESQCSDRTSRPSESSSSLNTTSSTYKNTSTRNLQKPQNTGILNSGKGLLQKLNFVKKRKSFKQPSGVICSHYNSNVQNRLKGQNSHQQR mdm1_yeast Structural protein MDM1 MDM1 YML104C YM8339.15C Mitochondrial distribution and morphology protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPKFPQFRLILVLFYLISMIQWSVITFSLGFFLNVCIFAYFVFFKSLPDLPKPQPRFVDIVPESSNTVDVDKELKSVEGLIQDGNAQIGKELESIVNLIIKDFVQPWFTKIDKNSDAEFLKVIKWRLLQTLLVVKDKLMKNDSASLIVLKLLPIFNKHFSTFCDAREAVLSDLTLERHKSANIDLQIAVEFNKNYKIHKSLSLKPNALQKEIEKSIRKTVIGLLPHLFDNDELDSLLVFTLMTEVLTTCIISPLIFKFTDPDSWNLRIVSLSQNYFEEKHKVHKIRRMLSKELQDHRKVMNDVANKDVGEPSSEKLELNAEYTGKQFEHYLNQLDSLLDLSDIKYVAYSLALKIYQLKENEHLTKENLKYKKRLLLSLNLIESKLSFPGSEIDTASKKLAREANYPDLNMDNGIVLKEMASFLTSITLKDIVDDSEFLPFFESFLGSVPETQGSTFLEYSQTIESFKNPLEDATSEDIISGYSGISTMQLQEISSKFFHNNNLQNMKLLDEGLVKNIILFRNSFQINNDEDTFILARKSVLLLQTEAIKYLDDRFLPLFKKTPSFLKMLSTSHIISTDIYAHFLSRIGGVNNPEQNKIIKDNVKTDFMNPVRIFANPGITDALDNIVNGSGSKPHKSRISSNPRYSQLFGSENDNIFKDKLFDDENDNTSEISVVEDQLDHPRNMEKVSVSSGNSGLNPSQFYGSNNFRDNIASLTISIDQIEKELELLRHLILKADLTNNQMQLKILKKSQRTLLKELEMKELLKQQYMVQENGNSLFRKTKIYIRSYFSENSSNGLKEITYYIINIHHFNNGQVSSWDMARRYNEFFELNTYLKKNFRDLMRQLQDLFPSKVKMSLKYHVTKTLLYEERKQKLEKYLRELLSISEICEDNIFRRFLTDPTPFKLNKEYMHDDILEEPLHEPIGSSNSTSNSSSVVDLQSSEDGGELNFYEDERHFFTDSGYPFYSQNKSFVKQICDLFISLFALNKANAGWLRGRAIITVLQQLLGSTIEKYIKVSIQKLRSEDQVFEAIVTFKNMLWGDNGLFERKRNETAEATRSEGERLRTEQLALTSLQRLFADTCGRVVGLRDSHEAAGRVHAMLQNPYLNASLLLEALDAILLDIICND rax1_yeast Bud site selection protein RAX1 RAX1 Revert to axial protein 1 YOR301W protein protein yeast Saccharomyces cerevisiae Baker's yeast MKEELSKVSSMQNFEMIQRERLPTLYEVLIQRTSQPVDLWTFYTFLSQFPYAINYLDFWVDLMTHTRLCKNYIELVRKSLINFPQEQQQNGSTSTATFDLLNALIEEGHLDPEAPDKLLENSGPDVPFSPKLNELLGDWKHQSGIGQEALRNEDVALIVDEIMKRRSQQDGKPQITTKQLLHSAVGLCNTYLVSPEQSERYLSNIPMETRNRIIESVQIERKYDIEIFDDLKNLTYQFLEMDCFPKFLSRVALHNIHDEISDWRFHSVGVTNEKSNRSRGQTHISRSPFSNHTSISRIGFGLLWLGIGFWIGYVLIFLAYSRAIRVVTVVPFTLGCYCIVCGMYQVDIVYSWFGVTQRLLHRHKNAGNDEGDASSDTDHVPMILAVFGGRRRLTRIEHPFTRQLLRKRGLWCLLLVVGATAAFTVIFSCVPGRRV gpa1-sst2 682771 N/A 682772 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 682773 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2B Interaction is enhanced by the addition of GDP-AIF4-. gpa1-rgs2 682771 N/A 682774 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 682773 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2B gpa1-mdm1 682771 N/A 682775 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 682773 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2B gpa1-rax1 682771 N/A 682773 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 682776 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2B