UniProt UniProt http://www.uniprot.org fox-2005-2 P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip antibody detection identification by antibody antibody detection Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk Mol. Biol. Cell (1059-1524) 2005 fox-2005-1 P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip fingerprinting peptide massfingerprinting fingerprinting Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk Mol. Biol. Cell (1059-1524) 2005 fox-2005-3 P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk Mol. Biol. Cell (1059-1524) 2005 fox-2005-4 P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Fox AH., Bond CS., Lamond AI. His-tagged PSPC1 and untagged NONO fragments have been coexpressed in E.coli.. Lysates have been loaded to Ni-agarose and subsequently gel filtration to purify His-tagged PSPC1 was applied. a.i.lamond@dundee.ac.uk Mol. Biol. Cell (1059-1524) 2005 fox-2005-5 P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Fox AH., Bond CS., Lamond AI. Cells have been treated with actinomycin D. a.i.lamond@dundee.ac.uk Mol. Biol. Cell (1059-1524) 2005 pspc1_human Paraspeckle component 1 PSPC1 PSP1 Paraspeckle protein 1 protein protein human Homo sapiens Human MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGEPAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLRHEEEHRRREEEMIRHREQEELRRQQEGFKPNYMENREQEMRMGDMGPRGAINMGDAFSPAPAGNQGPPPMMGMNMNNRATIPGPPMGPGPAMGPEGAANMGTPMMPDNGAVHNDRFPQGPPSQMGSPMGSRTGSETPQAPMSGVGPVSGGPGGFGRGSQGGNFEGPNKRRRY nono_human Non-POU domain-containing octamer-binding protein NONO NRB54 54 kDa nuclear RNA- and DNA-binding protein p54(nrb) 55 kDa nuclear protein NMT55 DNA-binding p52/p100 complex, 52 kDa subunit protein protein human Homo sapiens Human MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY sfpq_human Splicing factor, proline- and glutamine-rich SFPQ PSF Polypyrimidine tract-binding protein-associated-splicing factor DNA-binding p52/p100 complex, 100 kDa subunit 100 kDa DNA-pairing protein protein protein human Homo sapiens Human MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF nono-pspc1-1 664645 N/A 664650 unspecified role unspecified role prey prey N/A 664651 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association false false false 5D nono-nono-1 664645 N/A 664651 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 664651 unspecified role unspecified role prey prey physical association physical association false false false 5D pspc1-nono-2 664645 N/A 664650 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 664651 unspecified role unspecified role prey prey physical association physical association false false false 6 pspc1-nono-1 664646 N/A 664650 unspecified role unspecified role bait bait N/A 664651 unspecified role unspecified role prey prey physical association physical association false false false 5B Increasing the stringency of washing the beads did not change the interaction therefore appears to be stable. pspc1-nono-3 664647 N/A 664650 unspecified role unspecified role bait bait N/A 664651 unspecified role unspecified role prey prey physical association physical association false false false 8A Inhibition of PolII transcription did not change the interaction. pspc1-nono-4 664648 N/A 664650 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 664651 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 8B pspc1-sfpq-1 664649 N/A 664650 unspecified role unspecified role neutral component neutral component N/A 664652 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 colocalization colocalization false false false 7D