IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ palmer-2005-2 PCTAIRE protein kinases interact directly with the COPII complex and modulate secretory cargo transport. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Palmer KJ., Konkel JE., Stephens DJ. david.stephens@bristol.ac.uk Adult human brain cDNA J. Cell. Sci. (0021-9533) 2005 palmer-2005-1 PCTAIRE protein kinases interact directly with the COPII complex and modulate secretory cargo transport. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Palmer KJ., Konkel JE., Stephens DJ. david.stephens@bristol.ac.uk Adult human brain cDNA J. Cell. Sci. (0021-9533) 2005 palmer-2005-3 PCTAIRE protein kinases interact directly with the COPII complex and modulate secretory cargo transport. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Palmer KJ., Konkel JE., Stephens DJ. david.stephens@bristol.ac.uk Adult human brain cDNA J. Cell. Sci. (0021-9533) 2005 palmer-2005-4 PCTAIRE protein kinases interact directly with the COPII complex and modulate secretory cargo transport. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Palmer KJ., Konkel JE., Stephens DJ. david.stephens@bristol.ac.uk Adult human brain cDNA J. Cell. Sci. (0021-9533) 2005 palmer-2005-5 PCTAIRE protein kinases interact directly with the COPII complex and modulate secretory cargo transport. canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Palmer KJ., Konkel JE., Stephens DJ. david.stephens@bristol.ac.uk Adult human brain cDNA J. Cell. Sci. (0021-9533) 2005 cdk18_human Cyclin-dependent kinase 18 CDK18 PCTAIRE-motif protein kinase 3 PCTK3 Serine/threonine-protein kinase PCTAIRE-3 Cell division protein kinase 18 PCTAIRE3 protein protein human Homo sapiens Human MIMNKMKNFKRRFSLSVPRTETIEESLAEFTEQFNQLHNRRNENLQLGPLGRDPPQECSTFSPTDSGEEPGQLSPGVQFQRRQNQRRFSMEDVSKRLSLPMDIRLPQEFLQKLQMESPDLPKPLSRMSRRASLSDIGFGKLETYVKLDKLGEGTYATVFKGRSKLTENLVALKEIRLEHEEGAPCTAIREVSLLKNLKHANIVTLHDLIHTDRSLTLVFEYLDSDLKQYLDHCGNLMSMHNVKIFMFQLLRGLAYCHHRKILHRDLKPQNLLINERGELKLADFGLARAKSVPTKTYSNEVVTLWYRPPDVLLGSTEYSTPIDMWGVGCIHYEMATGRPLFPGSTVKEELHLIFRLLGTPTEETWPGVTAFSEFRTYSFPCYLPQPLINHAPRLDTDGIHLLSSLLLYESKSRMSAEAALSHSYFRSLGERVHQLEDTASIFSLKEIQLQKDPGYRGLAFQQPGRGKNRRQSIF sc23a_human Protein transport protein Sec23A SEC23A SEC23-related protein A protein protein human Homo sapiens Human MTTYLEFIQQNEERDGVRFSWNVWPSSRLEATRMVVPVAALFTPLKERPDLPPIQYEPVLCSRTTCRAVLNPLCQVDYRAKLWACNFCYQRNQFPPSYAGISELNQPAELLPQFSSIEYVVLRGPQMPLIFLYVVDTCMEDEDLQALKESMQMSLSLLPPTALVGLITFGRMVQVHELGCEGISKSYVFRGTKDLSAKQLQEMLGLSKVPLTQATRGPQVQQPPPSNRFLQPVQKIDMNLTDLLGELQRDPWPVPQGKRPLRSSGVALSIAVGLLECTFPNTGARIMMFIGGPATQGPGMVVGDELKTPIRSWHDIDKDNAKYVKKGTKHFEALANRAATTGHVIDIYACALDQTGLLEMKCCPNLTGGYMVMGDSFNTSLFKQTFQRVFTKDMHGQFKMGFGGTLEIKTSREIKISGAIGPCVSLNSKGPCVSENEIGTGGTCQWKICGLSPTTTLAIYFEVVNQHNAPIPQGGRGAIQFVTQYQHSSGQRRIRVTTIARNWADAQTQIQNIAASFDQEAAAILMARLAIYRAETEEGPDVLRWLDRQLIRLCQKFGEYHKDDPSSFRFSETFSLYPQFMFHLRRSSFLQVFNNSPDESSYYRHHFMRQDLTQSLIMIQPILYAYSFSGPPEPVLLDSSSILADRILLMDTFFQILIYHGETIAQWRKSGYQDMPEYENFRHLLQAPVDDAQEILHSRFPMPRYIDTEHGGSQARFLLSKVNPSQTHNNMYAWGQESGAPILTDDVSLQVFMDHLKKLAVSSAA sc24d_human Protein transport protein Sec24D KIAA0755 SEC24D SEC24-related protein D protein protein human Homo sapiens Human MSQQGYVATPPYSQPQPGIGLSPPHYGHYGDPSHTASPTGMMKPAGPLGATATRGMLPPGPPPPGPHQFGQNGAHATGHPPQRFPGPPPVNNVASSHAPYQPSAQSSYPGPISTSSVTQLGSQLSAMQINSYGSGMAPPSQGPPGPLSATSLQTPPRPPQPSILQPGSQVLPPPPTTLNGPGASPLPLPMYRPDGLSGPPPPNAQYQPPPLPGQTLGAGYPPQQANSGPQMAGAQLSYPGGFPGGPAQMAGPPQPQKKLDPDSIPSPIQVIENDRASRGGQVYATNTRGQIPPLVTTDCMIQDQGNASPRFIRCTTYCFPCTSDMAKQAQIPLAAVIKPFATIPSNESPLYLVNHGESGPVRCNRCKAYMCPFMQFIEGGRRYQCGFCNCVNDVPPFYFQHLDHIGRRLDHYEKPELSLGSYEYVATLDYCRKSKPPNPPAFIFMIDVSYSNIKNGLVKLICEELKTMLEKIPKEEQEETSAIRVGFITYNKVLHFFNVKSNLAQPQMMVVTDVGEVFVPLLDGFLVNYQESQSVIHNLLDQIPDMFADSNENETVFAPVIQAGMEALKAADCPGKLFIFHSSLPTAEAPGKLKNRDDKKLVNTDKEKILFQPQTNVYDSLAKDCVAHGCSVTLFLFPSQYVDVASLGLVPQLTGGTLYKYNNFQMHLDRQQFLNDLRNDIEKKIGFDAIMRVRTSTGFRATDFFGGILMNNTTDVEMAAIDCDKAVTVEFKHDDKLSEDSGALIQCAVLYTTISGQRRLRIHNLGLNCSSQLADLYKSCETDALINFFAKSAFKAVLHQPLKVIREILVNQTAHMLACYRKNCASPSAASQLILPDSMKVLPVYMNCLLKNCVLLSRPEISTDERAYQRQLVMTMGVADSQLFFYPQLLPIHTLDVKSTMLPAAVRCSESRLSEEGIFLLANGLHMFLWLGVSSPPELIQGIFNVPSFAHINTDMTLLPEVGNPYSQQLRMIMGIIQQKRPYSMKLTIVKQREQPEMVFRQFLVEDKGLYGGSSYVDFLCCVHKEICQLLN cdk16_human Cyclin-dependent kinase 16 PCTAIRE-motif protein kinase 1 CDK16 PCTK1 Serine/threonine-protein kinase PCTAIRE-1 Cell division protein kinase 16 PCTAIRE1 protein protein human Homo sapiens Human MDRMKKIKRQLSMTLRGGRGIDKTNGAPEQIGLDESGGGGGSDPGEAPTRAAPGELRSARGPLSSAPEIVHEDLKMGSDGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPEGYLEKLTLNSPIFDKPLSRRLRRVSLSEIGFGKLETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFKTYNYPKYRAEALLSHAPRLDSDGADLLTKLLQFEGRNRISAEDAMKHPFFLSLGERIHKLPDTTSIFALKEIQLQKEASLRSSSMPDSGRPAFRVVDTEF f1pby2_canlf CDK18 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris LTMNKMKNFKRRLSLSVPRTETIEESLTEFTEQFNQLHNRRNEDLQLGPLSRDPQPETSTFSPTDSGEDPGQPSPGMQYRRQNQRRFSMEDISKRLSLPMDIRLPQEFLQKLQLESPDLPKPLSRMSRRASLSDIGFGKLETYVKLDKLGEGTYATVFKGRSKLTENLVALKEIRLEHEEGAPCTAIREVSLLKNLKHANIVTLHDLIHTERSLTLVFEYLDSDLKQYLDHCGNLMSMHNVKIFMFQLLRGLAYCHRRKILHRDLKPQNLLISERGELKLADFGLARAKSVPTKTYSNEVVTLWYRPPDVLLGSTEYSTPIDMWGVGCIHYEMATGRPLFPGTVKEELQLIILTLLDTDGLHLLSSLLLYESKSRMSAEAALSHPYFRSLGERVHQLEDTASIFSLKEIQLQKDPGYRGLAFQQPGRGKSRRQSIF sec23a-pctk3-2 Interaction between SEC23A and PCTK3 identified by Y2H 596746 N/A 596751 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 596752 unspecified role unspecified role prey prey physical association physical association false false false The central region of PCTK3 shown to interact with SEC23A is common to all isoforms of PCTK3 known at time of publication. The interaction was not observed with full length PTCK3. Fig 1A, B, C sec24d-pctk3-1 Interaction between SEC24D and PCTK3 identified by Y2H 596746 n/a 596753 unspecified role unspecified role prey prey N/A 596751 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The interaction was observed to weaker than that between PCTK3 and SEC24A. The interaction was not observed with full length PTCK3. Fig 1A, B, C sec23a-pctk1-1 Interaction between SEC23A and PCTK1 identified by Y2H 596746 N/A 596754 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 596752 unspecified role unspecified role prey prey physical association physical association false false false The interacting region of PCTK1 has been inferred by alignment to PCTK3. The interaction was not observed with full length PTCK3. Fig 1C sec24d-pctk1-1 Interaction between SEC24D and PCTK1 identified by Y2H 596746 n/a 596753 unspecified role unspecified role prey prey N/A 596754 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The interacting region of PCTK1 has been inferred by alignment to PCTK3. The interaction was not observed with full length PTCK1. Fig 1C sec23a-pctk3-1 Interaction between SEC23A and PCTK3 identified by Y2H 596747 N/A 596752 unspecified role unspecified role bait bait N/A 596751 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false sec23a-pctk1-2 Interaction between SEC23A and PCTK1 identified by GST pulldown 596748 N/A 596752 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 596754 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 2 sec23a-pctk1-3 Interaction between SEC23A and PCTK1 identified by coimmunoprecipitation 596749 N/A 596754 unspecified role unspecified role bait bait ecobb Escherichia coli (strain B / BL21) N/A 596752 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false Fig 3B sec23a-pctk3-3 Interaction between SEC23A and canine PCTK3 identified by Y2H 596750 N/A 596755 unspecified role unspecified role bait bait N/A 596752 unspecified role unspecified role prey prey physical association physical association false false false Fig 3C