IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ neiditch-2005-1 Regulation of LuxPQ receptor activity by the quorum-sensing signal autoinducer-2. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Neiditch MB., Federle MJ., Miller ST., Bassler BL., Hughson FM. hughson@princeton.edu Mol. Cell (1097-2765) 2005 neiditch-2005-2 Regulation of LuxPQ receptor activity by the quorum-sensing signal autoinducer-2. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Neiditch MB., Federle MJ., Miller ST., Bassler BL., Hughson FM. hughson@princeton.edu Mol. Cell (1097-2765) 2005 neiditch-2005-3 Regulation of LuxPQ receptor activity by the quorum-sensing signal autoinducer-2. ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Neiditch MB., Federle MJ., Miller ST., Bassler BL., Hughson FM. hughson@princeton.edu Mol. Cell (1097-2765) 2005 luxp_vibha Autoinducer 2-binding periplasmic protein LuxP luxP protein protein vibha Vibrio harveyi MKKALLFSLISMVGFSPASQATQVLNGYWGYQEFLDEFPEQRNLTNALSEAVRAQPVPLSKPTQRPIKISVVYPGQQVSDYWVRNIASFEKRLYKLNINYQLNQVFTRPNADIKQQSLSLMEALKSKSDYLIFTLDTTRHRKFVEHVLDSTNTKLILQNITTPVREWDKHQPFLYVGFDHAEGSRELATEFGKFFPKHTYYSVLYFSEGYISDVRGDTFIHQVNRDNNFELQSAYYTKATKQSGYDAAKASLAKHPDVDFIYACSTDVALGAVDALAELGREDIMINGWGGGSAELDAIQKGDLDITVMRMNDDTGIAMAEAIKWDLEDKPVPTVYSGDFEIVTKADSPERIEALKKRAFRYSDN luxq_vibha Autoinducer 2 sensor kinase/phosphatase LuxQ luxQ protein protein vibha Vibrio harveyi MTTTRSNIKKRRSLATLITKIIILVLAPIILGIFIQSYYFSKQIIWQEVDRTKQQTSALIHNIFDSHFAAIQIHHDSNSKSEVIRDFYTDRDTDVLNFFFLSIDQSDPSHTPEFRFLTDHKGIIWDDGNAHFYGVNDLILDSLANRVSFSNNWYYINVMTSIGSRHMLVRRVPILDPSTGEVLGFSFNAVVLDNNFALMEKLKSESNVDNVVLVANSVPLANSLIGDEPYNVADVLQRKSSDKRLDKLLVIETPIVVNAVTTELCLLTVQDNQSVVTLQIQHILAMLASIIGMIMIALMSREWIESKVSAQLESLMSYTRSAREEKGFERFGGSDIEEFDHIGSTLESTFEELEAQKKSFRDLFNFALSPIMVWSEESVLIQMNPAARKELVIEDDHEIMHPVFQGFKEKLTPHLKMAAQGATLTGVNVPIGNKIYRWNLSPIRVDGDISGIIVQGQDITTLIEAEKQSNIARREAEKSAQARADFLAKMSHEIRTPINGILGVAQLLKDSVDTQEQKNQIDVLCHSGEHLLAVLNDILDFSKIEQGKFNIQKHPFSFTDTMRTLENIYRPICTNKGVELVIENELDPNVEIFTDQVRLNQILFNLVSNAVKFTPIGSIRLHAELEQFYGAENSVLVVELTDTGIGIESDKLDQMFEPFVQEESTTTREYGGSGLGLTIVKNLVDMLEGDVQVRSSKGGGTTFVITLPVKDRERVLRPLEVSQRIKPEALFDESLKVLLVEDNHTNAFILQAFCKKYKMQVDWAKDGLDAMELLSDTTYDLILMDNQLPHLGGIETTHEIRQNLRLGTPIYACTADTAKETSDAFMAAGANYVMLKPIKENALHEAFVDFKQRFLVERT luxp-luxq-3 591974 N/A 591977 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 591978 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Table 1 working 20.6 percent, free 22.2 percent 1.94 A PDB entry has GlySer at the n terminus of the P54302. luxp-luxq-4 591975 N/A 591977 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 591978 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 direct interaction direct interaction false false false 1C, 1D luxp-luxp 591976 N/A 591977 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 direct interaction direct interaction false false false 1C, 1D