UniProt UniProt http://www.uniprot.org venkatram-2005-1 Mto2p, a novel fission yeast protein required for cytoplasmic microtubule organization and anchoring of the cytokinetic actin ring. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Venkatram S., Jennings JL., Link A., Gould KL. kathy.gould@vanderbilt.edu kathy.gould@vabderbilt.edu Mol. Biol. Cell (1059-1524) 2005 venkatram-2005-2 Mto2p, a novel fission yeast protein required for cytoplasmic microtubule organization and anchoring of the cytokinetic actin ring. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Venkatram S., Jennings JL., Link A., Gould KL. kathy.gould@vanderbilt.edu kathy.gould@vabderbilt.edu Mol. Biol. Cell (1059-1524) 2005 venkatram-2005-5 Mto2p, a novel fission yeast protein required for cytoplasmic microtubule organization and anchoring of the cytokinetic actin ring. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast fluorescence fluorescence technology fluorescence tag visualisation tag visualisation Venkatram S., Jennings JL., Link A., Gould KL. kathy.gould@vanderbilt.edu kathy.gould@vabderbilt.edu Mol. Biol. Cell (1059-1524) 2005 venkatram-2005-3 Mto2p, a novel fission yeast protein required for cytoplasmic microtubule organization and anchoring of the cytokinetic actin ring. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Venkatram S., Jennings JL., Link A., Gould KL. kathy.gould@vanderbilt.edu kathy.gould@vabderbilt.edu Mol. Biol. Cell (1059-1524) 2005 venkatram-2005-4 Mto2p, a novel fission yeast protein required for cytoplasmic microtubule organization and anchoring of the cytokinetic actin ring. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Venkatram S., Jennings JL., Link A., Gould KL. kathy.gould@vanderbilt.edu kathy.gould@vabderbilt.edu Mol. Biol. Cell (1059-1524) 2005 yhp6_schpo Uncharacterized protein C902.06 SPBC902.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSEHNYQSDREVAEDPFLNYEASANQLSSNSRESTPRGSPWRAGMRSASLMTEPLEDSMYSDNNYLDNGVSFTKDENPLYSPSWPSLADANVNSMKSNNAIQEHKAAKFVSEKSLEKVSTADNNLVLQELENLRERLNQVELQLSERPSSYLGYHNNLSPYRSPNSYPSLLPSTHSPHSPAPLSTMQTALMRLRTYHPSPIILKPVEQAVNHAITLVNTSPSSVVDALCRSLAELCLGLVQEAIDASILSQQESSNSLDLVRHTPPLNYTSSVDSSPQRMASDSYGRPSLHLNDPFPSVDLQSNELSHHNVRTTLFSDDSRFHSKIHTHSTPPSQMYSAASHFRYRSDPSTRHVSNSTNKSSLHPSPTSLRVAHPIIPQRASPASQSFPSLQDTPSP mto1_schpo Protein MTO1 homolog, mitochondrial mto1 SPBC30B4.06c pi079 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFRTTKRYFCTSFNRRKNVVVIGGGHAGVEAAAAASRLGAKTTLLTKSFDNIGQMSCNPAFGGIGKGTLMREIDALGGVVSGVCDESAIQFHMLNRSNGPAVWSPRAQMDRSVFKKNMQKTISTYRKNLQVREGAAVSINVLTEDDGKQVCDSIVLEDGTAIPASCIVITTGTFLGGQINVGLTQLAAGRYGERPSLPLSKCLSNLGFKMGRLKTGTPPRLSSPINISKMTEQTGDEIPETFSFLNLERDFSPALPQRSCYRTYTTELTHEIVRKNLAFAPHMLAGDILSPRYCPSLEAKVTRFPHKARHLIWLEPEGLDPNSWWYPNGLSNSMPEEIQHNIIRSIPGLENCNIVRPAYGVMYDYVIPTQLKATLETKKIQGLYLAGQINGTTGYEEAAAQGILAGLNAGLSALGREPVDIPRNTALLGVMVDDLITKGVKEPYRVFTSRSEYRLTTRADNADLRLTPLAQSIGLLDDQTHWESFQRTKSLLDFSNKIAKEFILSPQQWSKLGMPIPNDGKYRSAWDLLSFTNLDLFCVISCIPKLKDIPKRVLQRLIIEGKYTYYIKRQGTQNKQLNCRDESTVIPSDFDFDTLHSVSAEELMLLKTIRPATIGQLKRIQGIKPGTIIRLLRHTYYNPAKEAWLRKVYMPMYDMHSSEENQSTNL tba2_schpo Tubulin alpha-2 chain tub1 alp2 atb2 SPBC800.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDSMEVDYMEEEY cdc11_schpo Septation initiation network scaffold protein cdc11 Cell division control protein 11 cdc11 SPCC1739.11c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEQLWLEHDLSEEWIPQPQEQGSDNSSEPPTTSNVNNTQSTGRGSSGTSTEHGTFKKGRNDAPDVPQWKQVNAKNPVARDIFARLDLENMFEESSKQSPPSKSPTKNPSKKSSNNSSRRSSSSVGKLSNVSNMQSSPSKDPFVSQDYEKESISSSQFSKKYSEGSLKSQQSNTRSNSVHEKQNTDHASNASSSSSVVSSPSLKPNNTSPLKLFQGASDPFTREHLNQLTQDVKSNSFENGEKQFSLPEPRRPQKPMRTTERKASLNTKDLYQEVEEVMARLRGRMPNSGRESTIFLPRKLSGLREEEEQDEISVEVSQEDSSNAFPSLSDQLHLKSLQSMKRVTSIFNDNDDSFPSASSSPQRQAYMTDKMPLREIDVGSSQSSSKTARLNSSPKSTLKTSSVKTRRSHSAQSSRKVSDYPNMVVITPADLPEGIDTTQGSMEFDRIHNRWRRKGHDSDLGFDFETDEDASLSHPERTILFKAASTRHQANNDPNNLEKQQPHSFPLRKQNVAQSEFPKHSLRDNSENAPQILSSFHDLSLQNESFDEMFNGRYENGSPIPFISSGSGLKSKADKDAEYSFSVSRQSIIQILSDVEPYEPFWKRIIQLDISRRHLDSLIGLSELCPSIEELTLEGNEIAYLTGCPVTIRDLNAVENRLSSLTSFSNLLNLQYLDISYNQLEDLTGLSSLIHLRELKVDSNHLWSLDGIQHLDGLLKLSACNNRIKELSFTNSNLHRLEELLLGNNEIEEIEEISSLQNLMVLQLDNNKLTNLKASQPMIHLRILRISNNAIHQLEVDQFPHLRTLYMDLNRFNRPPDIRRLKRLVNFSFRTQDPEASNFVIQPSLDIRNLYLSNNTFVTLDCKHMFLGVRYLELANVQLKEVPKYIATSMPNLRVLDLSHNYISDIESLKPLQMIHRLYLVGNRIKKMRNLCDILANLKQLNVLDLRMNPLNFNIYPVIDDSIYELSAASKYQQSINQKGHHRKEDPQKQWQEKELAFSSTLSEAWRTRRKMYAEAILLACPHLEWLDGSDVSQSSRAAFTKSSN mto1-mto2-3 682300 N/A 682305 unspecified role unspecified role prey prey N/A 682306 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false mto2-mto1-4 682300 N/A 682305 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 682306 unspecified role unspecified role prey prey physical association physical association false false false mto1-mto2-4 682301 N/A 682305 unspecified role unspecified role prey prey c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 682306 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 1A mto2-mto1-5 682301 N/A 682306 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 682305 unspecified role unspecified role bait bait c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 1A atb2-mto2-1 682302 N/A 682307 unspecified role unspecified role neutral component neutral component n-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 682305 unspecified role unspecified role neutral component neutral component c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 2B cdc11-mto2-1 682302 N/A 682308 unspecified role unspecified role neutral component neutral component c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 682305 unspecified role unspecified role neutral component neutral component c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 2C mto2-mto1-6 682303 N/A 682306 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false schpm Schizosaccharomyces pombe (fission yeast) Fission yeast N/A 682305 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 1B mto1-mto2-5 682304 N/A 682306 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) N/A 682305 unspecified role unspecified role prey prey region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 1C