UniProt UniProt http://www.uniprot.org samejima-2005-2 Fission yeast mto2p regulates microtubule nucleation by the centrosomin-related protein mto1p. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Samejima I., Lourenco PC., Snaith HA., Sawin KE. ken.sawin@ed.ac.uk Mol. Biol. Cell (1059-1524) 2005 samejima-2005-4 Fission yeast mto2p regulates microtubule nucleation by the centrosomin-related protein mto1p. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Samejima I., Lourenco PC., Snaith HA., Sawin KE. ken.sawin@ed.ac.uk Mol. Biol. Cell (1059-1524) 2005 samejima-2005-1 Fission yeast mto2p regulates microtubule nucleation by the centrosomin-related protein mto1p. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Samejima I., Lourenco PC., Snaith HA., Sawin KE. ken.sawin@ed.ac.uk Mol. Biol. Cell (1059-1524) 2005 samejima-2005-3 Fission yeast mto2p regulates microtubule nucleation by the centrosomin-related protein mto1p. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Samejima I., Lourenco PC., Snaith HA., Sawin KE. ken.sawin@ed.ac.uk Mol. Biol. Cell (1059-1524) 2005 yhp6_schpo Uncharacterized protein C902.06 SPBC902.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSEHNYQSDREVAEDPFLNYEASANQLSSNSRESTPRGSPWRAGMRSASLMTEPLEDSMYSDNNYLDNGVSFTKDENPLYSPSWPSLADANVNSMKSNNAIQEHKAAKFVSEKSLEKVSTADNNLVLQELENLRERLNQVELQLSERPSSYLGYHNNLSPYRSPNSYPSLLPSTHSPHSPAPLSTMQTALMRLRTYHPSPIILKPVEQAVNHAITLVNTSPSSVVDALCRSLAELCLGLVQEAIDASILSQQESSNSLDLVRHTPPLNYTSSVDSSPQRMASDSYGRPSLHLNDPFPSVDLQSNELSHHNVRTTLFSDDSRFHSKIHTHSTPPSQMYSAASHFRYRSDPSTRHVSNSTNKSSLHPSPTSLRVAHPIIPQRASPASQSFPSLQDTPSP mto1_schpo Protein MTO1 homolog, mitochondrial mto1 SPBC30B4.06c pi079 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFRTTKRYFCTSFNRRKNVVVIGGGHAGVEAAAAASRLGAKTTLLTKSFDNIGQMSCNPAFGGIGKGTLMREIDALGGVVSGVCDESAIQFHMLNRSNGPAVWSPRAQMDRSVFKKNMQKTISTYRKNLQVREGAAVSINVLTEDDGKQVCDSIVLEDGTAIPASCIVITTGTFLGGQINVGLTQLAAGRYGERPSLPLSKCLSNLGFKMGRLKTGTPPRLSSPINISKMTEQTGDEIPETFSFLNLERDFSPALPQRSCYRTYTTELTHEIVRKNLAFAPHMLAGDILSPRYCPSLEAKVTRFPHKARHLIWLEPEGLDPNSWWYPNGLSNSMPEEIQHNIIRSIPGLENCNIVRPAYGVMYDYVIPTQLKATLETKKIQGLYLAGQINGTTGYEEAAAQGILAGLNAGLSALGREPVDIPRNTALLGVMVDDLITKGVKEPYRVFTSRSEYRLTTRADNADLRLTPLAQSIGLLDDQTHWESFQRTKSLLDFSNKIAKEFILSPQQWSKLGMPIPNDGKYRSAWDLLSFTNLDLFCVISCIPKLKDIPKRVLQRLIIEGKYTYYIKRQGTQNKQLNCRDESTVIPSDFDFDTLHSVSAEELMLLKTIRPATIGQLKRIQGIKPGTIIRLLRHTYYNPAKEAWLRKVYMPMYDMHSSEENQSTNL tbg_schpo Tubulin gamma chain Gamma-tubulin tug1 gtb1 SPBC32F12.04 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGREIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYEACEDPNYLSL alp4_schpo Spindle pole body component alp4 Altered polarity protein 4 alp4 SPBC365.15 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVRSISSVLAKEESENGSPIPALEEGSVYHVSLKSEGVSPFSDRIQLNYLYDGKTFSDPSNLNIHQQEACLINELLNAFMGMEGVFVHLQDMKASSEFETIIMPPSFYILPGFDLGIKDIASEMLEMGSHYLSITAFIESRSHFEYGFVNHALCAALRKFVMDYVVLIMQCENQSRIDPNFSLQTLRLYTLPTSRSLRQVYLILRDLLLSMEKNASSSDDLGLSNIDDLLEQLNEGNDISHVVNATRSKKKVCKGGQVISFLTESLTKYAGDPVARKILTYLLREASRPYTKMLNEWIHLGLVNDPYDEFMIKIHKGITSMQLDEDYTDEYWEKRYVIREDQVPPQLLDLQNKVLFAGKYLNVVLECRKGVNNLASLNAKDDTQNQLLWPSTFDDDNFTLNIMNAYVYANESLLQLLQSSQSLYAHLYSLKHYFFLDQSDFFTTFLDNAQHELRKPAKYISITKLQSQLDLALRQPGTITATDPHKEYVTVEVNQTSLIDWLMHIVSISGLEEGTSSQGNEVWNESITKQADVGNETRNFESEHNRSTQGTSKVGSDKDINGFETMQLCYKVPFPLSLILSRKAIIRYQLLFRYFLLLRHVEMQLENSWVQHSKNSAWRLNSSNAKIEQWKRNSWLLRTRMLSFVQKIIYYTTSEVIETHWGKFMGELENARTVDNLMQEHIDFLDTCLKECMLTNSRLLKVQSKLLNTCAMFASYTSTFTRSLYLLENSEESFDEGRMDKMEEILRRYEDSFSRHLKSLVNACNYFASTETAALLSLVMKLTG tba2_schpo Tubulin alpha-2 chain tub1 alp2 atb2 SPBC800.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDSMEVDYMEEEY mto1-mto2-1 682261 N/A 682265 unspecified role unspecified role prey prey N/A 682266 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 5B, 5C mto1-mto2-2 682261 N/A 682267 unspecified role unspecified role prey prey N/A 682268 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 682266 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 682265 unspecified role unspecified role prey prey association association false false false Fig 8 mto2-mto1-3 682262 N/A 682265 unspecified role unspecified role neutral component neutral component c-terminus Undetermined feature position cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 682266 unspecified role unspecified role neutral component neutral component c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 4B atb2-mto1-1 682262 N/A 682269 unspecified role unspecified role neutral component neutral component n-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 682266 unspecified role unspecified role neutral component neutral component c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig 7E, 7F mto2-mto1-1 682263 N/A 682266 unspecified role unspecified role prey prey N/A 682265 unspecified role unspecified role bait bait physical association physical association false false false Fig 5A mto2-mto1-2 682264 N/A 682265 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) N/A 682266 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false Fig 5D, 5E